A common ragweed population resistant to cloransulam-methyl

William L. Patzoldt, Patrick J Tranel, Anita L. Alexander, Paul R. Schmitzer

Research output: Contribution to journalArticle

Abstract

A population of common ragweed not controlled by an acetolactate synthase (ALS)inhibiting herbicide, cloransulam-methyl, was sampled near Dunkirk, IN, the first year of the herbicides commercialization in 1998. Resistance in the Dunkirk population was confirmed by treating greenhouse-grown seedlings with cloransulammethyl. ALS activity assays and DNA sequencing were used to identify the resistance mechanism. ALS isolated from plants of the Dunkirk population exhibited an R/S ratio for cloransulam-methyl of > 5,000 when compared to ALS activity of populations from Claire City, SD, and V & J Seed Farms. R/S ratios of 4,100 and 110 were observed for two other ALS-inhibiting herbicides, chlorimuron and imazaquin, respectively. DNA sequencing revealed that an inferred leucine for tryptophan substitution at amino acid position 574 in ALS was responsible for the observed herbicide resistance. Additionally, DNA sequencing revealed significant variability among common ragweed ALS alleles. Two fragments of ALS were sequenced from three plants each of the Claire City and Dunkirk populations, totaling 688 nucleotide base pairs, of which 72 were polymorphic.

Original languageEnglish (US)
Pages (from-to)485-490
Number of pages6
JournalWeed Science
Volume49
Issue number4
DOIs
StatePublished - Jan 1 2001

Fingerprint

cloransulam
acetolactate synthase
sequence analysis
herbicides
imazaquin
chlorimuron
herbicide resistance
commercialization
resistance mechanisms
tryptophan
leucine
nucleotides
alleles
greenhouses
farms

Keywords

  • Acetolactate synthase
  • DNA polymorphism
  • Herbicide resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

A common ragweed population resistant to cloransulam-methyl. / Patzoldt, William L.; Tranel, Patrick J; Alexander, Anita L.; Schmitzer, Paul R.

In: Weed Science, Vol. 49, No. 4, 01.01.2001, p. 485-490.

Research output: Contribution to journalArticle

Patzoldt, William L. ; Tranel, Patrick J ; Alexander, Anita L. ; Schmitzer, Paul R. / A common ragweed population resistant to cloransulam-methyl. In: Weed Science. 2001 ; Vol. 49, No. 4. pp. 485-490.
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