A combined enhanced-fluorescence and label-free imaging instrument

I. D. Block, P. C. Mathias, N. Ganesh, B. T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photonic crystals have previously been shown to enhance fluorescence output of surface-bound dyes by two orders of magnitude and to enable sensitive label-free biomolecular detection. In this work we describe the design and characterization of a microscope capable of generating both label-free and enhanced-fluorescence images on engineered photonic crystal surfaces. We demonstrate 328x fluorescence enhancement, 41x signalto-noise improvements, and a reduction in the detection limit of a protein-conjugated fluorophore by over two orders of magnitude. We also characterize the resolution of enhanced fluorescence and label-free imaging to be submicron and 3 microns, respectively.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages726-729
Number of pages4
DOIs
StatePublished - Dec 11 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Fingerprint

Labels
Fluorescence
Imaging techniques
Photonic crystals
Fluorophores
Microscopes
Dyes
Proteins

Keywords

  • Enhanced fluorescence
  • Fluorescence microscopy
  • Guided-mode resonance
  • Label-free imaging
  • Photonic crystals

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Block, I. D., Mathias, P. C., Ganesh, N., & Cunningham, B. T. (2009). A combined enhanced-fluorescence and label-free imaging instrument. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 726-729). [5285426] (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285426

A combined enhanced-fluorescence and label-free imaging instrument. / Block, I. D.; Mathias, P. C.; Ganesh, N.; Cunningham, B. T.

TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 726-729 5285426 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Block, ID, Mathias, PC, Ganesh, N & Cunningham, BT 2009, A combined enhanced-fluorescence and label-free imaging instrument. in TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems., 5285426, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 726-729, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, CO, United States, 6/21/09. https://doi.org/10.1109/SENSOR.2009.5285426
Block ID, Mathias PC, Ganesh N, Cunningham BT. A combined enhanced-fluorescence and label-free imaging instrument. In TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. p. 726-729. 5285426. (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2009.5285426
Block, I. D. ; Mathias, P. C. ; Ganesh, N. ; Cunningham, B. T. / A combined enhanced-fluorescence and label-free imaging instrument. TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems. 2009. pp. 726-729 (TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems).
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