@inproceedings{de801cff08bc482da118dbf8aa2fed1d,
title = "A combined enhanced-fluorescence and label-free imaging instrument",
abstract = "Photonic crystals have previously been shown to enhance fluorescence output of surface-bound dyes by two orders of magnitude and to enable sensitive label-free biomolecular detection. In this work we describe the design and characterization of a microscope capable of generating both label-free and enhanced-fluorescence images on engineered photonic crystal surfaces. We demonstrate 328x fluorescence enhancement, 41x signalto-noise improvements, and a reduction in the detection limit of a protein-conjugated fluorophore by over two orders of magnitude. We also characterize the resolution of enhanced fluorescence and label-free imaging to be submicron and 3 microns, respectively.",
keywords = "Enhanced fluorescence, Fluorescence microscopy, Guided-mode resonance, Label-free imaging, Photonic crystals",
author = "Block, {I. D.} and Mathias, {P. C.} and N. Ganesh and Cunningham, {B. T.}",
year = "2009",
month = dec,
day = "11",
doi = "10.1109/SENSOR.2009.5285426",
language = "English (US)",
isbn = "9781424441938",
series = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
pages = "726--729",
booktitle = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
note = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 21-06-2009 Through 25-06-2009",
}