Abstract

Photonic crystals have previously been shown to enhance fluorescence output of surface-bound dyes by two orders of magnitude and to enable sensitive label-free biomolecular detection. In this work we describe the design and characterization of a microscope capable of generating both label-free and enhanced-fluorescence images on engineered photonic crystal surfaces. We demonstrate 328x fluorescence enhancement, 41x signalto-noise improvements, and a reduction in the detection limit of a protein-conjugated fluorophore by over two orders of magnitude. We also characterize the resolution of enhanced fluorescence and label-free imaging to be submicron and 3 microns, respectively.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages726-729
Number of pages4
DOIs
StatePublished - Dec 11 2009
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: Jun 21 2009Jun 25 2009

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

OtherTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
CountryUnited States
CityDenver, CO
Period6/21/096/25/09

Keywords

  • Enhanced fluorescence
  • Fluorescence microscopy
  • Guided-mode resonance
  • Label-free imaging
  • Photonic crystals

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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