A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1

Amanda C. Nottbohm, Robin S. Dothager, Karson S. Putt, Mirth T. Hoyt, Paul J. Hergenrother

Research output: Contribution to journalArticlepeer-review


(Chemical Equation Presented) Color me yellow: Poly(ADP-ribose) polymerases (PARPs) play a major role in cellular survival and maintenance of energy stores after genotoxic insult. The colorimetric PARP substrate ADP-ribose-pNP can be used to monitor PARP activity. By monitoring the production of p-nitrophenolate, the kinetic parameters of PARP-1, tankyrase, and PARP-4 could be evaluated. ADP = adenosine diphosphate, pNP = p-nitrophenoxy.

Original languageEnglish (US)
Pages (from-to)2066-2069
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number12
StatePublished - Jun 29 2007


  • Enzyme assays
  • Enzyme kinetics
  • Enzymes
  • Polymerases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)


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