A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1

Amanda C. Nottbohm; Robin S. Dothager; Karson S. Putt; Mirth T. Hoyt; Paul J. Hergenrother

doi:10.1002/anie.200603988

A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1

Amanda C. Nottbohm, Robin S. Dothager, Karson S. Putt, Mirth T. Hoyt, Paul J. Hergenrother

Research output: Contribution to journal › Article › peer-review

Abstract

(Chemical Equation Presented) Color me yellow: Poly(ADP-ribose) polymerases (PARPs) play a major role in cellular survival and maintenance of energy stores after genotoxic insult. The colorimetric PARP substrate ADP-ribose-pNP can be used to monitor PARP activity. By monitoring the production of p-nitrophenolate, the kinetic parameters of PARP-1, tankyrase, and PARP-4 could be evaluated. ADP = adenosine diphosphate, pNP = p-nitrophenoxy.

Original language	English (US)
Pages (from-to)	2066-2069
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	12
DOIs	https://doi.org/10.1002/anie.200603988
State	Published - 2007

Keywords

Enzyme assays
Enzyme kinetics
Enzymes
Polymerases

ASJC Scopus subject areas

Catalysis
General Chemistry

Online availability

10.1002/anie.200603988

Library availability

Discover UIUC Full Text

Cite this

@article{bea625811ea54db499b56f04c5c7439e,

title = "A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1",

abstract = "(Chemical Equation Presented) Color me yellow: Poly(ADP-ribose) polymerases (PARPs) play a major role in cellular survival and maintenance of energy stores after genotoxic insult. The colorimetric PARP substrate ADP-ribose-pNP can be used to monitor PARP activity. By monitoring the production of p-nitrophenolate, the kinetic parameters of PARP-1, tankyrase, and PARP-4 could be evaluated. ADP = adenosine diphosphate, pNP = p-nitrophenoxy.",

keywords = "Enzyme assays, Enzyme kinetics, Enzymes, Polymerases",

author = "Nottbohm, {Amanda C.} and Dothager, {Robin S.} and Putt, {Karson S.} and Hoyt, {Mirth T.} and Hergenrother, {Paul J.}",

year = "2007",

doi = "10.1002/anie.200603988",

language = "English (US)",

volume = "46",

pages = "2066--2069",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley & Sons, Ltd.",

number = "12",

}

TY - JOUR

T1 - A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1

AU - Nottbohm, Amanda C.

AU - Dothager, Robin S.

AU - Putt, Karson S.

AU - Hoyt, Mirth T.

AU - Hergenrother, Paul J.

PY - 2007

Y1 - 2007

N2 - (Chemical Equation Presented) Color me yellow: Poly(ADP-ribose) polymerases (PARPs) play a major role in cellular survival and maintenance of energy stores after genotoxic insult. The colorimetric PARP substrate ADP-ribose-pNP can be used to monitor PARP activity. By monitoring the production of p-nitrophenolate, the kinetic parameters of PARP-1, tankyrase, and PARP-4 could be evaluated. ADP = adenosine diphosphate, pNP = p-nitrophenoxy.

AB - (Chemical Equation Presented) Color me yellow: Poly(ADP-ribose) polymerases (PARPs) play a major role in cellular survival and maintenance of energy stores after genotoxic insult. The colorimetric PARP substrate ADP-ribose-pNP can be used to monitor PARP activity. By monitoring the production of p-nitrophenolate, the kinetic parameters of PARP-1, tankyrase, and PARP-4 could be evaluated. ADP = adenosine diphosphate, pNP = p-nitrophenoxy.

KW - Enzyme assays

KW - Enzyme kinetics

KW - Enzymes

KW - Polymerases

UR - http://www.scopus.com/inward/record.url?scp=34247558613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247558613&partnerID=8YFLogxK

U2 - 10.1002/anie.200603988

DO - 10.1002/anie.200603988

M3 - Article

C2 - 17299820

AN - SCOPUS:34247558613

SN - 1433-7851

VL - 46

SP - 2066

EP - 2069

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 12

ER -

A colorimetric substrate for poly(ADP-ribose) polymerase-1, VPARP, and tankyrase-1

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this