A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles

Juewen Liu, Yi Lu

Research output: Contribution to journalArticle

Abstract

A highly sensitive and selective colorimetric lead biosensor based on DNAzyme-directed assembly of gold nanoparticles is reported. It consists of a DNAzyme and its substrate that can hybridize to a 5-thio-modified DNA attached to gold nanoparticles. The hybridization brings gold nanoparticles together, resulting in a blue-colored nanoparticle assembly. In the presence of lead, the DNAzyme catalyzes specific hydrolytic cleavage, which prevents the formation of the nanoparticle assembly, resulting in red-colored individual nanoparticles. The detection level can be tuned to several orders of magnitude, from 100 nM to over 200 μM, through addition of an inactive variant of the DNAzyme. The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.

Original languageEnglish (US)
Pages (from-to)6642-6643
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number22
DOIs
StatePublished - Jun 4 2003

Fingerprint

Catalytic DNA
Biosensors
Gold
gold
Lead
Nanoparticles
Nucleic acids
nucleic acid
nanoparticle
Nanostructured materials
Nucleic Acids
cleavage
DNA
Enzymes
enzyme
sensor
substrate
Sensors
Substrates

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles. / Liu, Juewen; Lu, Yi.

In: Journal of the American Chemical Society, Vol. 125, No. 22, 04.06.2003, p. 6642-6643.

Research output: Contribution to journalArticle

@article{09fd9027f06547b1a2c9dde2dc03347c,
title = "A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles",
abstract = "A highly sensitive and selective colorimetric lead biosensor based on DNAzyme-directed assembly of gold nanoparticles is reported. It consists of a DNAzyme and its substrate that can hybridize to a 5-thio-modified DNA attached to gold nanoparticles. The hybridization brings gold nanoparticles together, resulting in a blue-colored nanoparticle assembly. In the presence of lead, the DNAzyme catalyzes specific hydrolytic cleavage, which prevents the formation of the nanoparticle assembly, resulting in red-colored individual nanoparticles. The detection level can be tuned to several orders of magnitude, from 100 nM to over 200 μM, through addition of an inactive variant of the DNAzyme. The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.",
author = "Juewen Liu and Yi Lu",
year = "2003",
month = "6",
day = "4",
doi = "10.1021/ja034775u",
language = "English (US)",
volume = "125",
pages = "6642--6643",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "22",

}

TY - JOUR

T1 - A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles

AU - Liu, Juewen

AU - Lu, Yi

PY - 2003/6/4

Y1 - 2003/6/4

N2 - A highly sensitive and selective colorimetric lead biosensor based on DNAzyme-directed assembly of gold nanoparticles is reported. It consists of a DNAzyme and its substrate that can hybridize to a 5-thio-modified DNA attached to gold nanoparticles. The hybridization brings gold nanoparticles together, resulting in a blue-colored nanoparticle assembly. In the presence of lead, the DNAzyme catalyzes specific hydrolytic cleavage, which prevents the formation of the nanoparticle assembly, resulting in red-colored individual nanoparticles. The detection level can be tuned to several orders of magnitude, from 100 nM to over 200 μM, through addition of an inactive variant of the DNAzyme. The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.

AB - A highly sensitive and selective colorimetric lead biosensor based on DNAzyme-directed assembly of gold nanoparticles is reported. It consists of a DNAzyme and its substrate that can hybridize to a 5-thio-modified DNA attached to gold nanoparticles. The hybridization brings gold nanoparticles together, resulting in a blue-colored nanoparticle assembly. In the presence of lead, the DNAzyme catalyzes specific hydrolytic cleavage, which prevents the formation of the nanoparticle assembly, resulting in red-colored individual nanoparticles. The detection level can be tuned to several orders of magnitude, from 100 nM to over 200 μM, through addition of an inactive variant of the DNAzyme. The concept developed here can be applied to the design of nucleic acid enzyme/nanoparticle sensors for analytes that are subject to in vitro selection, and thus can significantly expand the scope of nanomaterial applications and provide a novel approach to designing simple colorimetric biosensors.

UR - http://www.scopus.com/inward/record.url?scp=0038209225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038209225&partnerID=8YFLogxK

U2 - 10.1021/ja034775u

DO - 10.1021/ja034775u

M3 - Article

C2 - 12769568

AN - SCOPUS:0038209225

VL - 125

SP - 6642

EP - 6643

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 22

ER -