A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase

William L. Patzoldt, Aaron G. Hager, Joel S. McCormick, Patrick J. Tranel

Research output: Contribution to journalArticle

Abstract

Herbicides that act by inhibiting protoporphyrinogen oxidase (PPO) are widely used to control weeds in a variety of crops. The first weed to evolve resistance to PPO-inhibiting herbicides was Amaranthus tuberculatus, a problematic weed in the midwestern United States that previously had evolved multiple resistances to herbicides inhibiting two other target sites. Evaluation of a PPO-inhibitor-resistant A. tuberculatus biotype revealed that resistance was a (incompletely) dominant trait conferred by a single, nuclear gene. Three genes predicted to encode PPO were identified in A. tuberculatus. One gene from the resistant biotype, designated PPX2L, contained a codon deletion that was shown to confer resistance by complementation of a hemG mutant strain of Escherichia coli grown in the presence and absence of the PPO inhibitor lactofen. PPX2L is predicted to encode both plastid- and mitochondria-targeted PPO isoforms, allowing a mutation in a single gene to confer resistance to two herbicide target sites. Unique aspects of the resistance mechanism include an amino acid deletion, rather than a substitution, and the dual-targeting nature of the gene, which may explain why resistance to PPO inhibitors has been rare.

Original languageEnglish (US)
Pages (from-to)12329-12334
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number33
DOIs
StatePublished - Aug 15 2006

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Protoporphyrinogen Oxidase
Herbicide Resistance
Codon
Herbicides
Genes
Midwestern United States
Amaranthus
Plastids
Gene Targeting
Mitochondria
Protein Isoforms
Escherichia coli
Amino Acids
Mutation

Keywords

  • Amaranthus
  • Evolution
  • Herbicide resistance
  • Waterhemp
  • Weed resistance

ASJC Scopus subject areas

  • General

Cite this

A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase. / Patzoldt, William L.; Hager, Aaron G.; McCormick, Joel S.; Tranel, Patrick J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 33, 15.08.2006, p. 12329-12334.

Research output: Contribution to journalArticle

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