A "clickable" Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

Joomyung V. Jun; Conor M. Haney; Richard J. Karpowicz; Sam Giannakoulias; Virginia M.Y. Lee; E. James Petersson; David M. Chenoweth

doi:10.1021/jacs.8b13094

A "clickable" Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

Joomyung V. Jun, Conor M. Haney, Richard J. Karpowicz, Sam Giannakoulias, Virginia M.Y. Lee, E. James Petersson, David M. Chenoweth

Research output: Contribution to journal › Article › peer-review

Abstract

Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide-alkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules.

Original language	English (US)
Pages (from-to)	1893-1897
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	141
Issue number	5
DOIs	https://doi.org/10.1021/jacs.8b13094
State	Published - Feb 6 2019
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/jacs.8b13094

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AU - Jun, Joomyung V.

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AU - Karpowicz, Richard J.

AU - Giannakoulias, Sam

AU - Lee, Virginia M.Y.

AU - Petersson, E. James

AU - Chenoweth, David M.

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A "clickable" Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

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