@article{40a930f5009243b6995b9ad6f7dcb8a7,
title = "A chimeric influenza hemagglutinin delivered by parainfluenza virus 5 vector induces broadly protective immunity against genetically divergent influenza a H1 viruses in swine",
abstract = "Pigs are an important reservoir for human influenza viruses, and influenza causes significant economic loss to the swine industry. As demonstrated during the 2009 H1N1 pandemic, control of swine influenza virus infection is a critical step toward blocking emergence of human influenza virus. An effective vaccine that can induce broadly protective immunity against heterologous influenza virus strains is critically needed. In our previous studies [McCormick et al., 2015; PLoS One, 10(6):e0127649], we used molecular breeding (DNA shuffling) strategies to increase the breadth of the variable and conserved epitopes expressed within a single influenza A virus chimeric hemagglutinin (HA) protein. Chimeric HAs were constructed using parental HAs from the 2009 pandemic virus and swine influenza viruses that had a history of zoonotic transmission to humans. In the current study, we used parainfluenza virus 5 (PIV-5) as a vector to express one of these chimeric HA antigens, HA-113. Recombinant PIV-5 expressing HA-113 (PIV5-113) were rescued, and immunogenicity and protective efficacy were tested in both mouse and pig models. The results showed that PIV5-113 can protect mice and pigs against challenge with viruses expressing parental HAs. The protective immunity was extended against other genetically diversified influenza H1-expressing viruses. Our work demonstrates that PIV5-based influenza vaccines are efficacious as vaccines for pigs. The PIV5 vaccine vector and chimeric HA-113 antigen are discussed in the context of the development of universal influenza vaccines and the potential contribution of PIV5-113 as a candidate universal vaccine.",
keywords = "Influenza, Interspecies transmission, Mice, Pigs, Universal vaccine",
author = "Zhuo Li and Zaiser, {Sarah A.} and Pengcheng Shang and Heiden, {Dustin L.} and Heather Hajovsky and Pratik Katwal and Baylor DeVries and Jack Baker and Richt, {Juergen A.} and Yanhua Li and Biao He and Ying Fang and Huber, {Victor C.}",
note = "Funding Information: This work was supported by the National Pork Board (NPB Project #15-020; VCH, YF, and BH) and The National Institute of Allergy and Infectious Disease (NIAID) of the National Institutes of Health (NIH) under grant number R15AI090582-01 (YF and VCH) and contract numbers HHSN266200700005C (VCH; NIAID Centers of Excellence for Influenza Research and Surveillance) and HHSN272201400006C (JAR; NIAID Centers of Excellence for Influenza Research and Surveillance). Further support was provided by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences (NIGMS) of the NIH (P20GM103443, VCH, SZ, and DH), the NIAID (R44AI117976-01A1, VCH), and the BioSNTR which is supported by the National Science Foundation Established Program to Stimulate Competitive Research (NSF-EPSCoR) under grant number IIA-1355423 and the Governor's Office of Economic Development of the state of South Dakota (CBRC, JB and VCH). The work was also partially supported by endowment from Fred C. Davison Distinguished University Chair in Veterinary Medicine (BH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. Funding Information: This work was supported by the National Pork Board (NPB Project # 15-020 ; VCH, YF, and BH) and The National Institute of Allergy and Infectious Disease (NIAID) of the National Institutes of Health (NIH) under grant number R15AI090582-01 (YF and VCH) and contract numbers HHSN266200700005C (VCH; NIAID Centers of Excellence for Influenza Research and Surveillance ) and HHSN272201400006C (JAR; NIAID Centers of Excellence for Influenza Research and Surveillance). Further support was provided by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences (NIGMS) of the NIH (P20GM103443, VCH, SZ, and DH), the NIAID (R44AI117976-01A1, VCH), and the BioSNTR which is supported by the National Science Foundation Established Program to Stimulate Competitive Research (NSF-EPSCoR) under grant number IIA-1355423 and the Governor{\textquoteright}s Office of Economic Development of the state of South Dakota (CBRC, JB and VCH). The work was also partially supported by endowment from Fred C. Davison Distinguished University Chair in Veterinary Medicine (BH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2020",
month = nov,
doi = "10.1016/j.vetmic.2020.108859",
language = "English (US)",
volume = "250",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
}