A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy

Adam S. Backer, Mikael P. Backlund, Alexander R. Von Diezmann, Steffen J. Sahl, W. E. Moerner

Research output: Contribution to journalArticlepeer-review

Abstract

A phase mask design that we term a "bisected pupil" (BSP) provides several advantages for single-molecule optical imaging. When using the BSP with a dual-polarization optical Fourier processing system, both the position and dipole orientation of individual fluorescent molecules may be measured from a single camera image. In the context of single-molecule super-resolution microscopy, this technique permits one to diagnose, and subsequently to remove imaging artifacts resulting from orientation-induced localization errors. If the molecules labeling a structure are rotationally mobile, thus mitigating dipole orientation errors, this technique enables super-resolution imaging in three dimensions. We present simulations and experimental verification.

Original languageEnglish (US)
Article number193701
JournalApplied Physics Letters
Volume104
Issue number19
DOIs
StatePublished - May 12 2014
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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