Abstract
A common waterhemp biotype that was not controlled by triazine or acetolactate synthase (ALS)-inhibiting herbicides was isolated from a field in Bond County, IL, in the fall of 1996. Greenhouse and laboratory experiments determined resistance to atrazine and three ALS-inhibiting herbicides in this biotype. Based on whole-plant response, the Bond County common waterhemp biotype required over 1,000 times more imazethapyr relative to a susceptible biotype to reduce growth 50%. Cross-resistance to thifensulfuron, a sulfonylurea, and flumetsulam, a triazolopyrimidine sulfonanilide, was also detected. Based on in vivo enzyme assays, ALS in the Bond County common waterhemp biotype was 20-, > 8-, and 68-fold less sensitive than ALS in the susceptible biotype to imazethapyr, thifensulfuron, and flumetsulam, respectively. Whole-plant efficacy trials also indicated that the Bond County common waterhemp biotype required more than 20 kg ha-1 of atrazine to inhibit growth 50%. Chlorophyll fluorescence assays revealed that 100 nM atrazine inhibited photosynthesis in the susceptible biotype, whereas 10 M did not affect photosynthesis in the resistant biotype. Regions of the genes encoding ALS and D1 proteins were sequenced to determine the molecular basis for the resistances. Triazine resistance was conferred by a glycine for serine substitution at residue 264 of the D1 protein, while ALS resistance was conferred by a leucine for tryptophan substitution at residue 569 of ALS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 514-520 |
| Number of pages | 7 |
| Journal | Weed Science |
| Volume | 46 |
| Issue number | 5 |
| State | Published - Sep 1 1998 |
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Keywords
- AMATA
- Acetolactate synthase
- Cross-resistance
- Herbicide resistance
- Multiple resistance
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science
Cite this
A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. / Foes, Matthew J.; Liu, Lixin; Tranel, Patrick J; Wax, Loyd M.; Stoller, Edward W.
In: Weed Science, Vol. 46, No. 5, 01.09.1998, p. 514-520.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides
AU - Foes, Matthew J.
AU - Liu, Lixin
AU - Tranel, Patrick J
AU - Wax, Loyd M.
AU - Stoller, Edward W.
PY - 1998/9/1
Y1 - 1998/9/1
N2 - A common waterhemp biotype that was not controlled by triazine or acetolactate synthase (ALS)-inhibiting herbicides was isolated from a field in Bond County, IL, in the fall of 1996. Greenhouse and laboratory experiments determined resistance to atrazine and three ALS-inhibiting herbicides in this biotype. Based on whole-plant response, the Bond County common waterhemp biotype required over 1,000 times more imazethapyr relative to a susceptible biotype to reduce growth 50%. Cross-resistance to thifensulfuron, a sulfonylurea, and flumetsulam, a triazolopyrimidine sulfonanilide, was also detected. Based on in vivo enzyme assays, ALS in the Bond County common waterhemp biotype was 20-, > 8-, and 68-fold less sensitive than ALS in the susceptible biotype to imazethapyr, thifensulfuron, and flumetsulam, respectively. Whole-plant efficacy trials also indicated that the Bond County common waterhemp biotype required more than 20 kg ha-1 of atrazine to inhibit growth 50%. Chlorophyll fluorescence assays revealed that 100 nM atrazine inhibited photosynthesis in the susceptible biotype, whereas 10 M did not affect photosynthesis in the resistant biotype. Regions of the genes encoding ALS and D1 proteins were sequenced to determine the molecular basis for the resistances. Triazine resistance was conferred by a glycine for serine substitution at residue 264 of the D1 protein, while ALS resistance was conferred by a leucine for tryptophan substitution at residue 569 of ALS.
AB - A common waterhemp biotype that was not controlled by triazine or acetolactate synthase (ALS)-inhibiting herbicides was isolated from a field in Bond County, IL, in the fall of 1996. Greenhouse and laboratory experiments determined resistance to atrazine and three ALS-inhibiting herbicides in this biotype. Based on whole-plant response, the Bond County common waterhemp biotype required over 1,000 times more imazethapyr relative to a susceptible biotype to reduce growth 50%. Cross-resistance to thifensulfuron, a sulfonylurea, and flumetsulam, a triazolopyrimidine sulfonanilide, was also detected. Based on in vivo enzyme assays, ALS in the Bond County common waterhemp biotype was 20-, > 8-, and 68-fold less sensitive than ALS in the susceptible biotype to imazethapyr, thifensulfuron, and flumetsulam, respectively. Whole-plant efficacy trials also indicated that the Bond County common waterhemp biotype required more than 20 kg ha-1 of atrazine to inhibit growth 50%. Chlorophyll fluorescence assays revealed that 100 nM atrazine inhibited photosynthesis in the susceptible biotype, whereas 10 M did not affect photosynthesis in the resistant biotype. Regions of the genes encoding ALS and D1 proteins were sequenced to determine the molecular basis for the resistances. Triazine resistance was conferred by a glycine for serine substitution at residue 264 of the D1 protein, while ALS resistance was conferred by a leucine for tryptophan substitution at residue 569 of ALS.
KW - AMATA
KW - Acetolactate synthase
KW - Cross-resistance
KW - Herbicide resistance
KW - Multiple resistance
UR - http://www.scopus.com/inward/record.url?scp=0031785144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031785144&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0031785144
VL - 46
SP - 514
EP - 520
JO - Weed Science
JF - Weed Science
SN - 0043-1745
IS - 5
ER -