A BEN-domain-containing protein associates with heterochromatin and represses transcription

Kizhakke M. Sathyan, Zhen Shen, Vidisha Tripathi, Kannanganattu V. Prasanth, Supriya G. Prasanth

Research output: Contribution to journalArticle

Abstract

In eukaryotes, higher order chromatin structure governs crucial cellular processes including DNA replication, transcription and posttranscriptional gene regulation. Specific chromatin-interacting proteins play vital roles in the maintenance of chromatin structure. We have identified BEND3, a quadruple BEN domain-containing protein that is highly conserved amongst vertebrates. BEND3 colocalizes with HP1 and H3 trimethylated at K9 at heterochromatic regions in mammalian cells. Using an in vivo gene locus, we have been able to demonstrate that BEND3 associates with the locus only when it is heterochromatic and dissociates upon activation of transcription. Furthermore, tethering BEND3 inhibits transcription from the locus, indicating that BEND3 is involved in transcriptional repression through its interaction with histone deacetylases and Sall4, a transcription repressor. We further demonstrate that BEND3 is SUMOylated and that such modifications are essential for its role in transcriptional repression. Finally, overexpression of BEND3 causes premature chromatin condensation and extensive heterochromatinization, resulting in cell cycle arrest. Taken together, our data demonstrate the role of a novel heterochromatin-associated protein in transcriptional repression.

Original languageEnglish (US)
Pages (from-to)3149-3163
Number of pages15
JournalJournal of cell science
Volume124
Issue number18
DOIs
StatePublished - Sep 15 2011

Fingerprint

Heterochromatin
Chromatin
Histone Deacetylases
Cell Cycle Checkpoints
Eukaryota
DNA Replication
Transcriptional Activation
Genes
Vertebrates
Proteins
Maintenance
Protein Domains

Keywords

  • BEND3
  • Heterochromatin
  • SUMO
  • Transcriptional repression

ASJC Scopus subject areas

  • Cell Biology

Cite this

A BEN-domain-containing protein associates with heterochromatin and represses transcription. / Sathyan, Kizhakke M.; Shen, Zhen; Tripathi, Vidisha; Prasanth, Kannanganattu V.; Prasanth, Supriya G.

In: Journal of cell science, Vol. 124, No. 18, 15.09.2011, p. 3149-3163.

Research output: Contribution to journalArticle

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