A 3D imaging system integrating photoacoustic and fluorescence orthogonal projections for anatomical, functional and molecular assessment of rodent models

Hans P. Brecht, Vassili Ivanov, DIego S. Dumani, Stanislav Y. Emelianov, Mark A. Anastasio, Sergey A. Ermilov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a preclinical 3D imaging instrument integrating photoacoustic tomography and fluorescence (PAFT) addressing known deficiencies in sensitivity and spatial resolution of the individual imaging components. PAFT is designed for simultaneous acquisition of photoacoustic and fluorescence orthogonal projections at each rotational position of a biological object, enabling direct registration of the two imaging modalities. Orthogonal photoacoustic projections are utilized to reconstruct large (21 cm3) volumes showing vascularized anatomical structures and regions of induced optical contrast with spatial resolution exceeding 100 μm. The major advantage of orthogonal fluorescence projections is significant reduction of background noise associated with transmitted or backscattered photons. The fluorescence imaging component of PAFT is used to boost detection sensitivity by providing low-resolution spatial constraint for the fluorescent biomarkers. PAFT performance characteristics were assessed by imaging optical and fluorescent contrast agents in tissue mimicking phantoms and in vivo. The proposed PAFT technology will enable functional and molecular volumetric imaging using fluorescent biomarkers, nanoparticles, and other photosensitive constructs mapped with high fidelity over robust anatomical structures, such as skin, central and peripheral vasculature, and internal organs.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2018
EditorsLihong V. Wang, Alexander A. Oraevsky
PublisherSPIE
ISBN (Electronic)9781510614734
DOIs
StatePublished - Jan 1 2018
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2018 - San Francisco, United States
Duration: Jan 28 2018Feb 1 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10494
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2018
CountryUnited States
CitySan Francisco
Period1/28/182/1/18

Keywords

  • cancer imaging
  • molecular imaging
  • optical imaging
  • Photoacoustic tomography
  • preclinical imaging
  • quantitative imaging
  • small animal models

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Brecht, H. P., Ivanov, V., Dumani, DI. S., Emelianov, S. Y., Anastasio, M. A., & Ermilov, S. A. (2018). A 3D imaging system integrating photoacoustic and fluorescence orthogonal projections for anatomical, functional and molecular assessment of rodent models. In L. V. Wang, & A. A. Oraevsky (Eds.), Photons Plus Ultrasound: Imaging and Sensing 2018 [1049411] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10494). SPIE. https://doi.org/10.1117/12.2290880