TY - JOUR
T1 - A 1.7-Mb chromosomal inversion downstream of a PpOFP1 gene is responsible for flat fruit shape in peach
AU - Zhou, Hui
AU - Ma, Ruijuan
AU - Gao, Lei
AU - Zhang, Jinyun
AU - Zhang, Aidi
AU - Zhang, Xiujun
AU - Ren, Fei
AU - Zhang, Weihan
AU - Liao, Liao
AU - Yang, Qiurui
AU - Xu, Shengli
AU - Otieno Ogutu, Collins
AU - Zhao, Jianbo
AU - Yu, Mingliang
AU - Jiang, Quan
AU - Korban, Schuyler S.
AU - Han, Yuepeng
N1 - Funding Information:
We would like to thank Professor Liang Chen for his help in plant transformation. This project was supported by funds received from the National Key Research and Development Program (2019YFD1000200), the Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences (XDA24030404‐4), the National Natural Science Foundation of China (31672134 and 31601740), and the China Agriculture Research System (grant no. CARS‐30).
Publisher Copyright:
© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
PY - 2021/1
Y1 - 2021/1
N2 - Flat peaches have become popular worldwide due to their novelty and convenience. The peach flat fruit trait is genetically controlled by a single gene at the S locus, but its genetic basis remains unclear. Here, we report a 1.7-Mb chromosomal inversion downstream of a candidate gene encoding OVATE Family Protein, designated PpOFP1, as the causal mutation for the peach flat fruit trait. Genotyping of 727 peach cultivars revealed an occurrence of this large inversion in flat peaches, but absent in round peaches. Ectopic overexpression of PpOFP1 resulted in oval-shaped leaves and shortened siliques in Arabidopsis, suggesting its role in repressing cell elongation. Transcriptional activation of PpOFP1 by the chromosomal inversion may repress vertical elongation in flat-shaped fruits at early stages of development, resulting in the flat fruit shape. Moreover, PpOFP1 can interact with fruit elongation activator PpTRM17, suggesting a regulatory network controlling fruit shape in peach. Additionally, screening of peach wild relatives revealed an exclusive presence of the chromosomal inversion in P. ferganensis, supporting that this species is the ancestor of the domesticated peach. This study provides new insights into mechanisms underlying fruit shape evolution and molecular tools for genetic improvement of fruit shape trait in peach breeding programmes.
AB - Flat peaches have become popular worldwide due to their novelty and convenience. The peach flat fruit trait is genetically controlled by a single gene at the S locus, but its genetic basis remains unclear. Here, we report a 1.7-Mb chromosomal inversion downstream of a candidate gene encoding OVATE Family Protein, designated PpOFP1, as the causal mutation for the peach flat fruit trait. Genotyping of 727 peach cultivars revealed an occurrence of this large inversion in flat peaches, but absent in round peaches. Ectopic overexpression of PpOFP1 resulted in oval-shaped leaves and shortened siliques in Arabidopsis, suggesting its role in repressing cell elongation. Transcriptional activation of PpOFP1 by the chromosomal inversion may repress vertical elongation in flat-shaped fruits at early stages of development, resulting in the flat fruit shape. Moreover, PpOFP1 can interact with fruit elongation activator PpTRM17, suggesting a regulatory network controlling fruit shape in peach. Additionally, screening of peach wild relatives revealed an exclusive presence of the chromosomal inversion in P. ferganensis, supporting that this species is the ancestor of the domesticated peach. This study provides new insights into mechanisms underlying fruit shape evolution and molecular tools for genetic improvement of fruit shape trait in peach breeding programmes.
KW - Prunus
KW - TONNEAU1-recruiting motif protein
KW - chromosomal inversion
KW - flat peach
KW - ovate family protein
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U2 - 10.1111/pbi.13455
DO - 10.1111/pbi.13455
M3 - Article
C2 - 32722872
AN - SCOPUS:85089456660
VL - 19
SP - 192
EP - 205
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
SN - 1467-7644
IS - 1
ER -