A 1.7-Mb chromosomal inversion downstream of a PpOFP1 gene is responsible for flat fruit shape in peach

Hui Zhou, Ruijuan Ma, Lei Gao, Jinyun Zhang, Aidi Zhang, Xiujun Zhang, Fei Ren, Weihan Zhang, Liao Liao, Qiurui Yang, Shengli Xu, Collins Otieno Ogutu, Jianbo Zhao, Mingliang Yu, Quan Jiang, Schuyler S. Korban, Yuepeng Han

Research output: Contribution to journalArticlepeer-review

Abstract

Flat peaches have become popular worldwide due to their novelty and convenience. The peach flat fruit trait is genetically controlled by a single gene at the S locus, but its genetic basis remains unclear. Here, we report a 1.7-Mb chromosomal inversion downstream of a candidate gene encoding OVATE Family Protein, designated PpOFP1, as the causal mutation for the peach flat fruit trait. Genotyping of 727 peach cultivars revealed an occurrence of this large inversion in flat peaches, but absent in round peaches. Ectopic overexpression of PpOFP1 resulted in oval-shaped leaves and shortened siliques in Arabidopsis, suggesting its role in repressing cell elongation. Transcriptional activation of PpOFP1 by the chromosomal inversion may repress vertical elongation in flat-shaped fruits at early stages of development, resulting in the flat fruit shape. Moreover, PpOFP1 can interact with fruit elongation activator PpTRM17, suggesting a regulatory network controlling fruit shape in peach. Additionally, screening of peach wild relatives revealed an exclusive presence of the chromosomal inversion in P. ferganensis, supporting that this species is the ancestor of the domesticated peach. This study provides new insights into mechanisms underlying fruit shape evolution and molecular tools for genetic improvement of fruit shape trait in peach breeding programmes.

Original languageEnglish (US)
Pages (from-to)192-205
Number of pages14
JournalPlant Biotechnology Journal
Volume19
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • Prunus
  • TONNEAU1-recruiting motif protein
  • chromosomal inversion
  • flat peach
  • ovate family protein

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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