Find Research Outputs

Search results

• Article

  High throughput screening and selection methods for directed enzyme evolution

  Xiao, H., Bao, Z. & Zhao, H., Apr 29 2015, In: Industrial and Engineering Chemistry Research. 54, 16, p. 4011-4020 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Enzymes
  • Screening
  • Throughput
  • Costs

• Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., May 15 2015, In: ACS synthetic biology. 4, 5, p. 585-594 10 p.

  Research output: Contribution to journal › Article › peer-review

  • CRISPR-Associated Proteins
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • Proteins

• Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense

  Zhou, Y., Wei, Y., Nanjaraj Urs, A. N., Lin, L., Xu, T., Hu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Bioscience Reports. 39, 6, Y.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • sulfoacetaldehyde
  • Bifidobacterium
  • Taurine
  • Bacteria
  • Oxidoreductases

• Identification and Characterization of Citrus Peel Uronic Acid Oxidase

  Wei, Y., Tan, Y. L., Ang, E. L. & Zhao, H., Mar 16 2020, In: ChemBioChem. 21, 6, p. 797-800 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Uronic Acids
  • Citrus
  • Oxidoreductases
  • Pectins
  • citrus pectin

• Identification of an important motif that controls the activity and specificity of sugar transporters

  Wang, M., Yu, C. & Zhao, H., Jul 1 2016, In: Biotechnology and bioengineering. 113, 7, p. 1460-1467 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Sugars
  • Glucose
  • xylose-glucose
  • Facilitative Glucose Transport Proteins

• Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

  Wen, S., Tan, T. & Zhao, H., Dec 5 2012, In: Journal of Biotechnology. 164, 2, p. 248-253 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Yarrowia
  • Lipases
  • Lipase
  • Enzymes
  • Half-Life

• Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

  Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A. N., Liu, Y., Ang, E. L., Zhao, S., Zhao, H. & Zhang, Y., Dec 1 2018, In: Nature communications. 9, 1, 4224.

  Research output: Contribution to journal › Article › peer-review

  • Skatole
  • indoleacetic acid
  • fermentation
  • Carboxy-Lyases
  • tryptophan

• Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

  Abil, Z., Gumy, L. F., Zhao, H. & Hoogenraad, C. C., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 950-956 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Carrier Proteins
  • Messenger RNA
  • RNA
  • RNA-Binding Proteins
  • Ligands

• Insights into Cell-Free Conversion of CO₂ to Chemicals by a Multienzyme Cascade Reaction

  Singh, R. K., Singh, R., Sivakumar, D., Kondaveeti, S., Kim, T., Li, J., Sung, B. H., Cho, B. K., Kim, D. R., Kim, S. C., Kalia, V. C., Zhang, Y. H. P. J., Zhao, H., Kang, Y. C. & Lee, J. K., Dec 7 2018, In: ACS Catalysis. 8, 12, p. 11085-11093 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Oxidoreductases
  • NAD phosphite oxidoreductase
  • Methanol
  • glutathione-independent formaldehyde dehydrogenase
  • Formaldehyde

• In Situ Assembly of Antifouling/Bacterial Silver Nanoparticle-Hydrogel Composites with Controlled Particle Release and Matrix Softening

  Baek, K., Liang, J., Lim, W. T., Zhao, H., Kim, D. H. & Kong, H., Jul 22 2015, In: ACS Applied Materials and Interfaces. 7, 28, p. 15359-15367 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Silver nanoparticles
  • Hydrogels
  • Polyethylene Glycol Dimethacrylate Hydrogel
  • Gels
  • Composite materials

• Introduction

  Zhao, H., Aug 27 2013, In: Synthetic Biology.

  Research output: Contribution to journal › Article › peer-review

• Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via ¹³C metabolic flux analysis

  Feng, X. & Zhao, H., Sep 1 2013, In: AIChE Journal. 59, 9, p. 3195-3202 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Flux Analysis
  • xylose-glucose
  • Xylose
  • Metabolism
  • Yeast

• Investigating host dependence of xylose utilization in recombinant Saccharomyces cerevisiae strains using RNA-seq analysis

  Feng, X. & Zhao, H., 2013, In: Biotechnology for Biofuels. 6, 1, 96.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • RNA
  • Yeast
  • Saccharomyces cerevisiae
  • regulation

• Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via ¹³C metabolic flux analysis

  Feng, X. & Zhao, H., Nov 18 2013, In: Microbial cell factories. 12, 1, 114.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Flux Analysis
  • Xylose
  • Metabolism
  • Yeast
  • Saccharomyces cerevisiae

• Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

  Li, S., Ha, S. J., Kim, H. J., Galazka, J. M., Cate, J. H. D., Jin, Y. S. & Zhao, H., Oct 10 2013, In: Journal of Biotechnology. 168, 1, p. 1-6 6 p.

  Research output: Contribution to journal › Article › peer-review

  • aldose 1-epimerase
  • Cellobiose
  • Cell growth
  • Yeast
  • Glucose

• Investigation of the role of Arg301 identified in the X-ray structure of phosphite dehydrogenase

  Hung, J. E., Fogle, E. J., Christman, H. D., Johannes, T. W., Zhao, H., Metcalf, W. W. & Van Der Donk, W. A., May 29 2012, In: Biochemistry. 51, 21, p. 4254-4262 9 p.

  Research output: Contribution to journal › Article › peer-review

  • NAD phosphite oxidoreductase
  • Phosphites
  • X rays
  • Hydroxy Acids
  • X-Rays

• In vivo biosensors: mechanisms, development, and applications

  Shi, S., Ang, E. L. & Zhao, H., Jul 1 2018, In: Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 491-516 26 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosensing Techniques
  • Biosensors
  • Metabolic engineering
  • Synthetic biology
  • Synthetic Biology

• Manipulating natural product biosynthetic pathways via DNA assembler

  Shao, Z. & Zhao, H., 2014, In: Current protocols in chemical biology. 6, 2, p. 65-100 36 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthetic Pathways
  • Biological Products
  • spectinabilin
  • DNA
  • Synthetic Biology

• Mechanistic studies on the flavin:NADH reductase (PrnF) from Pseudomonas fluorescens involved in arylamine oxygenation

  Tiwari, M. K., Singh, R. K., Lee, J. K. & Zhao, H., Feb 1 2012, In: Bioorganic and Medicinal Chemistry Letters. 22, 3, p. 1344-1347 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Pseudomonas fluorescens
  • Oxygenation
  • 4,6-dinitro-o-cresol
  • Flavin-Adenine Dinucleotide
  • NAD

• Members of the rusc protein family interact with Sufu and inhibit vertebrate hedgehog signaling

  Jin, Z., Schwend, T., Fu, J., Bao, Z., Liang, J., Zhao, H., Mei, W. & Yang, J., Nov 1 2016, In: Development (Cambridge). 143, 21, p. 3944-3955 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Hedgehogs
  • vertebrate
  • Vertebrates
  • family
  • protein

• Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol

  Lian, J., Chao, R. & Zhao, H., May 2014, In: Metabolic Engineering. 23, p. 92-99 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Butylene Glycols
  • 2,3-butylene glycol
  • Metabolic engineering
  • Metabolic Engineering
  • Yeast

• Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae

  Shi, S., Si, T., Liu, Z., Zhang, H., Ang, E. L. & Zhao, H., May 10 2016, In: Scientific reports. 6, 25675.

  Research output: Contribution to journal › Article › peer-review

  • 1-Butanol
  • Metabolic Engineering
  • Saccharomyces cerevisiae
  • citramalate
  • Threonine

• Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production

  Feng, X., Lian, J. & Zhao, H., Jan 1 2015, In: Metabolic Engineering. 27, p. 10-19 10 p.

  Research output: Contribution to journal › Article › peer-review

  • cetyl alcohol
  • Metabolic engineering
  • Metabolic Engineering
  • Yeast
  • Saccharomyces cerevisiae

• Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose

  Guo, W., Sheng, J., Zhao, H. & Feng, X., Feb 1 2016, In: Microbial cell factories. 15, 1, 24.

  Research output: Contribution to journal › Article › peer-review

  • cetyl alcohol
  • Metabolic engineering
  • Xylose
  • Metabolic Engineering
  • Fatty Alcohols

• Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA

  Abil, Z., Denard, C. A. & Zhao, H., Mar 1 2014, In: Journal of Biological Engineering. 8, 1, 7.

  Research output: Contribution to journal › Article › peer-review

  • Protein Engineering
  • RNA
  • Proteins
  • RNA-Binding Proteins
  • Carrier Proteins

• Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

  Lian, J., Schultz, C., Cao, M., HamediRad, M. & Zhao, H., Dec 1 2019, In: Nature communications. 10, 1, 5794.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • genome
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • functionals
  • Genes
  • Throughput

• New N-acetyltransferase fold in the structure and mechanism of the phosphonate biosynthetic enzyme FrbF

  Bae, B., Cobb, R. E., DeSieno, M. A., Zhao, H. & Nair, S. K., Oct 14 2011, In: Journal of Biological Chemistry. 286, 41, p. 36132-36141 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Organophosphonates
  • Acetyltransferases
  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid
  • Enzymes
  • Cytidine Monophosphate

• New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

  Luo, Y., Enghiad, B. & Zhao, H., Feb 2016, In: Natural Product Reports. 33, 2, p. 174-182 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Multigene Family
  • Biological Products
  • Genes
  • Metabolic engineering
  • Synthetic biology

• Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease

  Sun, N., Liang, J., Abil, Z. & Zhao, H., Mar 1 2012, In: Molecular BioSystems. 8, 4, p. 1255-1263 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effectors
  • Sickle Cell Anemia
  • Gene Editing
  • Targeted Gene Repair
  • Therapeutics

• Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands

  Liang, J., Mclachlan, M. J. & Zhao, H., May 1 2013, In: Biotechnology and bioengineering. 110, 5, p. 1419-1429 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Switch Genes
  • Artificial Cells
  • Ligands
  • Gene expression
  • Cells

• Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae

  Eriksen, D. T., HamediRad, M., Yuan, Y. & Zhao, H., Jul 17 2015, In: ACS synthetic biology. 4, 7, p. 808-814 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthetic Pathways
  • Fatty acids
  • Yeast
  • Esters
  • Saccharomyces cerevisiae

• Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators

  Bao, Z., Jain, S., Jaroenpuntaruk, V. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 686-693 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Cells
  • Genes
  • Gene Regulatory Networks
  • Activator Appliances

• Photoenzymatic enantioselective intermolecular radical hydroalkylation

  Huang, X., Wang, B., Wang, Y., Jiang, G., Feng, J. & Zhao, H., Aug 6 2020, In: Nature. 584, 7819, p. 69-74 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Carbonyl compounds
  • Alkenes
  • Enzymes
  • Photoreactivity
  • Enantioselectivity

• Probing the molecular determinants of fluorinase specificity

  Yeo, W. L., Chew, X., Smith, D. J., Chan, K. P., Sun, H., Zhao, H., Lim, Y. H. & Ang, E. L., 2017, In: Chemical Communications. 53, 17, p. 2559-2562 4 p.

  Research output: Contribution to journal › Article › peer-review

  • fluorinase
  • 5'-chloro-5'-deoxyadenosine
  • Substrates

• Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis

  Zhang, H., Li, X., Su, X., Ang, E. L., Zhang, Y. & Zhao, H., Apr 20 2016, In: ChemCatChem. 8, 8, p. 1500-1506 7 p.

  Research output: Contribution to journal › Article › peer-review

  • sugar beets
  • Sugar beets
  • adipic acid
  • Catalysis
  • catalysis

• Production of long chain alcohols and alkanes upon coexpression of an acyl-ACP reductase and aldehyde-deformylating oxygenase with a bacterial type-I fatty acid synthase in E. coli

  Coursolle, D., Lian, J., Shanklin, J. & Zhao, H., Jun 17 2015, In: Molecular BioSystems. 11, 9, p. 2464-2472 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Type I Fatty Acid Synthase
  • Alkanes
  • Oxygenases
  • Aldehyde Reductase
  • Alcohols

• Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

  Si, T., Li, B., Comi, T. J., Wu, Y., Hu, P., Wu, Y., Min, Y., Mitchell, D. A., Zhao, H. & Sweedler, J. V., 2017, In: Journal of the American Chemical Society. 139, 36, p. 12466-12473 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Ionization
  • Mass spectrometry
  • Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
  • Desorption
  • ionization

• Programmable DNA-Guided Artificial Restriction Enzymes

  Enghiad, B. & Zhao, H., May 19 2017, In: ACS synthetic biology. 6, 5, p. 752-757 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Enzymes
  • DNA
  • Pyrococcus furiosus
  • Genetic engineering
  • Cloning

• Protein design for pathway engineering

  Eriksen, D. T., Lian, J. & Zhao, H., Feb 2014, In: Journal of Structural Biology. 185, 2, p. 234-242 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Engineering
  • Biocatalysis
  • Enzymes
  • Proteins
  • Protein Engineering

• Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system

  Fine, J. D., Shpigler, H. Y., Ray, A. M., Beach, N. J., Sankey, A. L., Cash-Ahmed, A., Huang, Z. Y., Astrauskaite, I., Chao, R., Zhao, H. & Robinson, G. E., Sep 2018, In: PloS one. 13, 9, e0203444.

  Research output: Contribution to journal › Article › peer-review

  • queen honey bees
  • Bees
  • Honey
  • Nutrition
  • Pollen

• Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria

  Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., N. Nanjaraj Urs, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Dec 1 2019, In: Nature communications. 10, 1, 1609.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • sulfonates
  • Anaerobic Bacteria
  • Bacteria
  • bacteria
  • cleavage

• Rapid and efficient galactose fermentation by engineered Saccharomyces cerevisiae

  Quarterman, J., Skerker, J. M., Feng, X., Liu, I. Y., Zhao, H., Arkin, A. P. & Jin, Y. S., Jul 10 2016, In: Journal of Biotechnology. 229, p. 13-21 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Yeast
  • Fermentation
  • Galactose
  • Saccharomyces cerevisiae
  • Ethanol

• Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

  Shao, Z., Luo, Y. & Zhao, H., Apr 1 2011, In: Molecular BioSystems. 7, 4, p. 1056-1059 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthetic Pathways
  • Biological Products
  • Synthetic Biology
  • Homologous Recombination
  • DNA

• Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli

  Ren, H., Biswas, S., Ho, S., Van Der Donk, W. A. & Zhao, H., Oct 19 2018, In: ACS chemical biology. 13, 10, p. 2966-2972 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Bacteriocins
  • Escherichia coli
  • enterocin
  • Genes
  • Glycosylation

• Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli

  Si, T., Tian, Q., Min, Y., Zhang, L., Sweedler, J. V., Van Der Donk, W. A. & Zhao, H., Sep 26 2018, In: Journal of the American Chemical Society. 140, 38, p. 11884-11888 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Protein Sorting Signals
  • Escherichia coli
  • peptide
  • Screening
  • screening

• Reconstruction of Lead Acid Battery Negative Electrodes after Hard Sulfation Using Controlled Chelation Chemistry

  Gossage, Z. T., Guo, F., Hatfield, K. O., Martin, T. A., Tian, Q., Gao, E. J., Kumar, A., Rodríguez-López, J. & Zhao, H., Jan 9 2020, In: Journal of the Electrochemical Society. 167, 12, 120537.

  Research output: Contribution to journal › Article › peer-review

  • Chelation
  • Lead acid batteries
  • Ethylenediaminetetraacetic acid
  • Edetic Acid
  • Electrodeposition

• Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold

  Shao, Z., Rao, G., Li, C., Abil, Z., Luo, Y. & Zhao, H., Nov 15 2013, In: ACS synthetic biology. 2, 11, p. 662-669 8 p.

  Research output: Contribution to journal › Article › peer-review

  • spectinabilin
  • Biosynthetic Pathways
  • Synthetic biology
  • Metabolites
  • Bioactivity

• Reversal of the β-oxidation cycle in saccharomyces cerevisiae for production of fuels and chemicals

  Lian, J. & Zhao, H., Mar 20 2015, In: ACS synthetic biology. 4, 3, p. 332-341 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Yeast
  • Saccharomyces cerevisiae
  • Oxidation
  • Fatty Acids
  • 1-Butanol

• RNAi-assisted genome evolution (RAGE) in Saccharomyces cerevisiae

  Si, T. & Zhao, H., Jan 1 2016, In: Methods in Molecular Biology. 1470, p. 183-198 16 p.

  Research output: Contribution to journal › Article › peer-review

  • RNA Interference
  • Saccharomyces cerevisiae
  • Genome
  • Acetic Acid
  • Libraries

• RNAi-assisted genome evolution in saccharomyces cerevisiae for complex phenotype engineering

  Si, T., Luo, Y., Bao, Z. & Zhao, H., Mar 20 2015, In: ACS synthetic biology. 4, 3, p. 283-291 9 p.

  Research output: Contribution to journal › Article › peer-review

  • RNA
  • Yeast
  • RNA Interference
  • Saccharomyces cerevisiae
  • Genes