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• A brief overview of synthetic biology research programs and roadmap studies in the United States

  Si, T. & Zhao, H., Dec 1 2016, In: Synthetic and Systems Biotechnology. 1, 4, p. 258-264 7 p.

  Research output: Contribution to journal › Review article › peer-review

  • Synthetic biology
  • Synthetic Biology
  • Government Agencies
  • Biological systems

• Accelerated genome engineering through multiplexing

  Bao, Z., Cobb, R. E. & Zhao, H., Jan 1 2016, In: Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 8, 1, p. 5-21 17 p.

  Research output: Contribution to journal › Review article › peer-review

  • Multiplexing
  • Genes
  • Genome
  • Bioengineering
  • Gene Editing

• A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

  Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In: Metabolic Engineering Communications. 9, e00101.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Genomics
  • Genes
  • Yeasts
  • Basidiomycota
  • Genome

• A continuing career in biocatalysis: Frances H. Arnold

  Fasan, R., Jennifer Kan, S. B. & Zhao, H., Nov 1 2019, In: ACS Catalysis. 9, 11, p. 9775-9788 14 p.

  Research output: Contribution to journal › Article › peer-review

  • Biocatalysis
  • Enzymes
  • Biocatalysts
  • Catalysis

• A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

  Sun, H., Zhao, H. & Ang, E. L., 2018, In: Chemical Communications. 54, 68, p. 9458-9461 4 p.

  Research output: Contribution to journal › Article › peer-review

  • fluorinase
  • Halogenation
  • Substrates
  • Enzymes

• ACS Catalysis and the Scope of Papers Sought in Three Catalysis Subdisciplines: Biocatalysis and Enzymology, Molecular Catalysis for Organic Synthesis, and Heterogeneous Photocatalysis

  Chang, S., Fornasiero, P., Gunnoe, T. B., Jones, C. W., Linic, S., Ooi, T., Williams, R. M. & Zhao, H., Jul 1 2016, In: ACS Catalysis. 6, 7, p. 4782-4785 4 p.

  Research output: Contribution to journal › Review article › peer-review

• Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster

  Luo, Y., Huang, H., Liang, J., Wang, M., Lu, L., Shao, Z., Cobb, R. E. & Zhao, H., Dec 5 2013, In: Nature communications. 4, 2894.

  Research output: Contribution to journal › Article › peer-review

  • genes
  • Multigene Family
  • Genes
  • Chemical activation
  • activation

• Activation of silent biosynthetic gene clusters using transcription factor decoys

  Wang, B., Guo, F., Dong, S. H. & Zhao, H., Feb 1 2019, In: Nature chemical biology. 15, 2, p. 111-114 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Multigene Family
  • Oxazoles
  • non-ribosomal peptide synthase
  • Polyketide Synthases
  • Transcription Factors

• Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

  Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In: Biotechnology Journal. 13, 9, 1700601.

  Research output: Contribution to journal › Review article › peer-review

  • Metabolic Engineering
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Saccharomyces cerevisiae
  • Gene Knockout Techniques
  • Biotechnology

• A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium

  Xing, M., Wei, Y., Hua, G., Li, M., Nanjaraj Urs, A. N., Wang, F., Hu, Y., Zhai, W., Liu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Aug 15 2019, In: Biochemical Journal. 476, 15, p. 2271-2279 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Taurine
  • Sulfur
  • Bacteria
  • Multigene Family
  • Genes

• A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

  Shi, S., Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., Jan 1 2016, In: Metabolic Engineering. 33, p. 19-27 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • 2,3-butylene glycol
  • Xylose

• Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering

  Desieno, M., Du, J. & Zhao, H., Mar 9 2011, Protein Engineering Handbook, Volume 1 & Volume 2. Wiley-VCH, Vol. 2. p. 777-796 20 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

  Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In: Nature chemical biology. 16, 4, p. 387-390 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Knock-In Techniques
  • Ribonucleoproteins
  • DNA
  • HEK293 Cells
  • Neoplastic Stem Cells

• A New Era of Genome Integration - Simply Cut and Paste!

  Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 601-609 9 p.

  Research output: Contribution to journal › Review article › peer-review

  • Ointments
  • Genes
  • Homologous Recombination
  • Genome
  • Synthetic Biology

• A new fluorescence complementation biosensor for detection of estrogenic compounds

  Mclachlan, M. J., Katzenellenbogen, J. A. & Zhao, H., Dec 2011, In: Biotechnology and bioengineering. 108, 12, p. 2794-2803 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Estrogens
  • Biosensing Techniques
  • Biosensors
  • Fluorescence
  • Ligands

• An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine

  Yin, J., Wei, Y., Liu, D., Hu, Y., Lu, Q., Lui Ang, E., Zhao, H. & Zhang, Y., Oct 25 2019, In: Journal of Biological Chemistry. 294, 43, p. 15662-15671 10 p.

  Research output: Contribution to journal › Article › peer-review

  • pyrimidine
  • Alanine
  • Nitrogen
  • Degradation
  • Bacteria

• A pathway for isethionate dissimilation in Bacillus krulwichiae

  Tong, Y., Wei, Y., Hu, Y., Ang, E. L., Zhao, H. & Zhang, Y., 2019, In: Applied and environmental microbiology. 85, 15, e00793-19.

  Research output: Contribution to journal › Article › peer-review

  • sulfoacetaldehyde
  • Bacillus (bacteria)
  • Bacillus
  • Bacteria
  • bacterium

• A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae

  Ren, H., Hu, P. & Zhao, H., Aug 2017, In: Biotechnology and bioengineering. 114, 8, p. 1847-1854 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Escherichia coli
  • Yeast
  • Workflow
  • Biological Products
  • Saccharomyces cerevisiae

• Applying advanced dna assembly methods to generate pathway libraries

  Eriksen, D. T., Chao, R. & Zhao, H., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 331-347 17 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Aryl-aldehyde formation in fungal polyketides: Discovery and characterization of a distinct biosynthetic mechanism

  Wang, M., Beissner, M. & Zhao, H., Feb 20 2014, In: Chemistry and Biology. 21, 2, p. 257-263 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Polyketide Synthases
  • Polyketides
  • Aldehydes
  • non-ribosomal peptide synthase
  • Aspergillus

• A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis

  Xiong, X., Zhang, Y., Yan, J., Jain, S., Chee, S., Ren, B. & Zhao, H., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 1034-1042 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Epitopes
  • Chromatin Immunoprecipitation
  • Genes
  • Chromatin
  • Throughput

• A single-chain TALEN architecture for genome engineering

  Sun, N. & Zhao, H., Mar 1 2014, In: Molecular BioSystems. 10, 3, p. 446-453 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Genome
  • DNA Cleavage
  • Peptides
  • Deoxyribonuclease I

• A step through the looking glass

  Liang, Y., Ang, E. L. & Zhao, H., Sep 1 2016, In: Science China Life Sciences. 59, 9, p. 979-980 2 p.

  Research output: Contribution to journal › Article › peer-review

• A synthetic device for intracellular mRNA transport and local protein translation in eukaryotes

  Abil, Z., Hoogenraad, C. & Zhao, H., 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, Vol. 1. p. 104-113 10 p.

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Carrier Proteins
  • RNA transport
  • Messenger RNA
  • Protein Biosynthesis
  • transport proteins

• A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases

  Sun, N. & Zhao, H., 2014, Homing Endonucleases: Methods and Protocols. Humana Press Inc., p. 87-96 10 p. (Methods in Molecular Biology; vol. 1123).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Endonucleases
  • Plasmids
  • DNA Cleavage
  • Double-Stranded DNA Breaks
  • Cell Survival

• A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency

  Jeya, M., Kim, T. S., Kumar Tiwari, M., Li, J., Zhao, H. & Lee, J. K., Dec 2012, In: Molecular BioSystems. 8, 12, p. 3103-3106 4 p.

  Research output: Contribution to journal › Article › peer-review

  • flavanone synthetase
  • Rhizobium etli
  • Pyrones
  • Malonyl Coenzyme A
  • Acetyl Coenzyme A

• Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation

  Lim, Y. H., Wong, F. T., Yeo, W. L., Ching, K. C., Lim, Y. W., Heng, E., Chen, S., Tsai, D. J., Lauderdale, T. L., Shia, K. S., Ho, Y. S., Hoon, S., Ang, E. L., Zhang, M. M. & Zhao, H., Aug 16 2018, In: ChemBioChem. 19, 16, p. 1716-1719 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Multigene Family
  • Genes
  • Chemical activation

• Automated multiplex genome-scale engineering in yeast

  Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., 2017, In: Nature communications. 8, 15187.

  Research output: Contribution to journal › Article › peer-review

  • genome
  • yeast
  • Yeast
  • Genes
  • Yeasts

• Biocatalysis for Drug Discovery and Development

  Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., May 13 2016, Green Biocatalysis. Wiley, p. 421-455 35 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Biocatalysis
  • Drug Discovery
  • Drug products
  • Enzymes
  • Biocatalysts

• Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

  Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In: Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biocatalysis
  • Enzymes
  • Pharmaceutical Preparations
  • Oxidoreductases
  • Isomerases

• Biocatalyst development by directed evolution

  Wang, M., Si, T. & Zhao, H., Jul 2012, In: Bioresource Technology. 115, p. 117-125 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Biocatalysts
  • Biocatalysis
  • organic solvent
  • Enzymes
  • mutant

• Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca

  Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Biochemical Journal. 476, 4, p. 733-746 14 p.

  Research output: Contribution to journal › Article › peer-review

  • sulfoacetaldehyde
  • Short Chain Dehydrogenase-Reductases
  • Klebsiella oxytoca
  • Taurine
  • NADP

• Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense

  Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In: Biochemical Journal. 476, 11, p. 1605-1619 15 p.

  Research output: Contribution to journal › Article › peer-review

  • Ketoglutaric Acids
  • Bifidobacterium
  • Pyridoxal
  • Taurine
  • Pyridoxal Phosphate

• Breaking the silence: new strategies for discovering novel natural products

  Ren, H., Wang, B. & Zhao, H., Dec 1 2017, In: Current Opinion in Biotechnology. 48, p. 21-27 7 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biological Products
  • Genes
  • Multigene Family
  • Throughput
  • Pharmaceutical Preparations

• Building a global alliance of biofoundries

  Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., Dec 1 2019, In: Nature communications. 10, 1, 2040.

  Research output: Contribution to journal › Comment/debate › peer-review

  Open Access

  • biotechnology
  • Biotechnology
  • organisms
  • Testing

• Cell surface display of functional human MHC class II proteins: Yeast display versus insect cell display

  Wen, F., Sethi, D. K., Wucherpfennig, K. W. & Zhao, H., Sep 2011, In: Protein Engineering, Design and Selection. 24, 9, p. 701-709 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Fungal Proteins
  • Peptides
  • Yeast
  • Histocompatibility Antigens Class II
  • Insecta

• Challenges and opportunities in synthetic biology for chemical engineers

  Luo, Y., Lee, J. K. & Zhao, H., Nov 5 2013, In: Chemical Engineering Science. 103, p. 115-119 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Synthetic Biology
  • Synthetic biology
  • Engineers
  • Modularization
  • Biofuels

• Characterization and application of the Fe(ii) and α-ketoglutarate dependent hydroxylase FrbJ

  Desieno, M. A., Van Der Donk, W. A. & Zhao, H., Sep 28 2011, In: Chemical Communications. 47, 36, p. 10025-10027 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Bioassay
  • FR 33289
  • Mixed Function Oxygenases
  • Antimalarials
  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid

• Characterization and engineering of the adenylation domain of a NRPS-like protein: A potential biocatalyst for aldehyde generation

  Wang, M. & Zhao, H., Apr 4 2014, In: ACS Catalysis. 4, 4, p. 1219-1225 7 p.

  Research output: Contribution to journal › Article › peer-review

  • non-ribosomal peptide synthase
  • Biocatalysts
  • Aldehydes
  • Peptides
  • Staphylococcal Protein A

• Characterization of bacillus subtilis colony biofilms via mass spectrometry and fluorescence imaging

  Si, T., Li, B., Zhang, K., Xu, Y., Zhao, H. & Sweedler, J. V., Jun 3 2016, In: Journal of Proteome Research. 15, 6, p. 1955-1962 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Fluorescence imaging
  • Bacilli
  • Biofilms
  • Mass spectrometry
  • Optical Imaging

• Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes

  Yeo, W. L., Heng, E., Tan, L. L., Lim, Y. W., Lim, Y. H., Hoon, S., Zhao, H., Zhang, M. M. & Wong, F. T., Sep 2019, In: Biotechnology and bioengineering. 116, 9, p. 2330-2338 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Editing
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Proteins
  • Genes
  • CRISPR-Cas Systems

• Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098

  Nguyen, K., DeSieno, M. A., Bae, B., Johannes, T. W., Cobb, R. E., Zhao, H. & Nair, S. K., 2019, In: Organic and Biomolecular Chemistry. 17, 6, p. 1506-1518 13 p.

  Research output: Contribution to journal › Article › peer-review

  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid
  • Biosynthesis
  • dimethylaniline monooxygenase (N-oxide forming)
  • biosynthesis
  • 4,6-dinitro-o-cresol

• Characterization of the N-oxygenase AurF from Streptomyces thioletus

  Chanco, E., Choi, Y. S., Sun, N., Vu, M. & Zhao, H., Oct 15 2014, In: Bioorganic and Medicinal Chemistry. 22, 20, p. 5569-5577 9 p.

  Research output: Contribution to journal › Article › peer-review

  • 4-nitrobenzoic acid
  • Methylphenazonium Methosulfate
  • Oxygenases
  • Streptomyces
  • aureothin

• Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in d-tagatose production

  Jagtap, S. S., Singh, R., Kang, Y. C., Zhao, H. & Lee, J. K., May 10 2014, In: Enzyme and Microbial Technology. 58-59, p. 44-51 8 p.

  Research output: Contribution to journal › Article › peer-review

  • galactitol 2-dehydrogenase
  • tagatose
  • Rhizobium
  • Oxidoreductases
  • Cloning

• Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae

  Sun, J., Shao, Z., Zhao, H., Nair, N., Wen, F., Xu, J. H. & Zhao, H., Aug 2012, In: Biotechnology and bioengineering. 109, 8, p. 2082-2092 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Cloning
  • Yeast
  • Saccharomyces cerevisiae
  • Organism Cloning
  • Xylans

• Cloning and characterization of a type III polyketide synthase from Aspergillus niger

  Li, J., Luo, Y., Lee, J. K. & Zhao, H., Oct 15 2011, In: Bioorganic and Medicinal Chemistry Letters. 21, 20, p. 6085-6089 5 p.

  Research output: Contribution to journal › Article › peer-review

  • flavanone synthetase
  • Aspergillus
  • Aspergillus niger
  • Cloning
  • benzoyl-coenzyme A

• CmlI is an N-oxygenase in the biosynthesis of chloramphenicol

  Lu, H., Chanco, E. & Zhao, H., Sep 16 2012, In: Tetrahedron. 68, 37, p. 7651-7654 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthesis
  • Oxygenases
  • Chloramphenicol
  • Enzymes
  • Ferrozine

• Combinatorial design of a highly efficient xylose-utilizing pathway in Saccharomyces cerevisiae for the production of cellulosic biofuels

  Kim, B., Du, J., Eriksen, D. T. & Zhao, H., Feb 2013, In: Applied and environmental microbiology. 79, 3, p. 931-941 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Biofuels
  • Xylose
  • biofuel
  • xylose
  • biofuels

• Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

  Lian, J., Hamedirad, M., Hu, S. & Zhao, H., Dec 1 2017, In: Nature communications. 8, 1, 1688.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic engineering
  • Metabolic Engineering
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • yeast
  • Yeast

• Combinatorial optimization of a highly efficient xylose utilization pathway in saccharomyces cerevisiae

  Du, J., Kim, B. & Zhao, H., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Xylose
  • Yeast
  • Combinatorial optimization
  • Pentoses
  • Enzymes