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A brief overview of synthetic biology research programs and roadmap studies in the United States
Si, T. & Zhao, H., Dec 1 2016, In: Synthetic and Systems Biotechnology. 1, 4, p. 258-264 7 p.Research output: Contribution to journal › Review article › peer-review
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Accelerated genome engineering through multiplexing
Bao, Z., Cobb, R. E. & Zhao, H., Jan 1 2016, In: Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 8, 1, p. 5-21 17 p.Research output: Contribution to journal › Review article › peer-review
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A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data
Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In: Metabolic Engineering Communications. 9, e00101.Research output: Contribution to journal › Article › peer-review
Open Access -
A continuing career in biocatalysis: Frances H. Arnold
Fasan, R., Jennifer Kan, S. B. & Zhao, H., Nov 1 2019, In: ACS Catalysis. 9, 11, p. 9775-9788 14 p.Research output: Contribution to journal › Article › peer-review
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A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance
Sun, H., Zhao, H. & Ang, E. L., 2018, In: Chemical Communications. 54, 68, p. 9458-9461 4 p.Research output: Contribution to journal › Article › peer-review
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ACS Catalysis and the Scope of Papers Sought in Three Catalysis Subdisciplines: Biocatalysis and Enzymology, Molecular Catalysis for Organic Synthesis, and Heterogeneous Photocatalysis
Chang, S., Fornasiero, P., Gunnoe, T. B., Jones, C. W., Linic, S., Ooi, T., Williams, R. M. & Zhao, H., Jul 1 2016, In: ACS Catalysis. 6, 7, p. 4782-4785 4 p.Research output: Contribution to journal › Review article › peer-review
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Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster
Luo, Y., Huang, H., Liang, J., Wang, M., Lu, L., Shao, Z., Cobb, R. E. & Zhao, H., Dec 5 2013, In: Nature communications. 4, 2894.Research output: Contribution to journal › Article › peer-review
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Activation of silent biosynthetic gene clusters using transcription factor decoys
Wang, B., Guo, F., Dong, S. H. & Zhao, H., Feb 1 2019, In: Nature chemical biology. 15, 2, p. 111-114 4 p.Research output: Contribution to journal › Article › peer-review
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Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In: Biotechnology Journal. 13, 9, 1700601.Research output: Contribution to journal › Review article › peer-review
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A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens
Wang, F., Wei, Y., Lu, Q., Ang, E. L., Zhao, H. & Zhang, Y., Jul 2020, In: Bioscience Reports. 40, 7, BSR20201642.Research output: Contribution to journal › Article › peer-review
Open Access -
A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium
Xing, M., Wei, Y., Hua, G., Li, M., Nanjaraj Urs, A. N., Wang, F., Hu, Y., Zhai, W., Liu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Aug 15 2019, In: Biochemical Journal. 476, 15, p. 2271-2279 9 p.Research output: Contribution to journal › Article › peer-review
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A genetic toolbox for metabolic engineering of Issatchenkia orientalis
Cao, M., Fatma, Z., Song, X., Hsieh, P. H., Tran, V. G., Lyon, W. L., Sayadi, M., Shao, Z., Yoshikuni, Y. & Zhao, H., May 2020, In: Metabolic Engineering. 59, p. 87-97 11 p.Research output: Contribution to journal › Article › peer-review
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A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae
Shi, S., Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., Jan 1 2016, In: Metabolic Engineering. 33, p. 19-27 9 p.Research output: Contribution to journal › Article › peer-review
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Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic
Badur, A. H., Jagtap, S. S., Yalamanchili, G., Lee, J. K., Zhao, H. & Rao, C. V., Jan 1 2015, In: Applied and environmental microbiology. 81, 5, p. 1865-1873 9 p.Research output: Contribution to journal › Article › peer-review
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Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering
Desieno, M., Du, J. & Zhao, H., Mar 9 2011, Protein Engineering Handbook, Volume 1 & Volume 2. Wiley-VCH, Vol. 2. p. 777-796 20 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter
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A mass spectrometry-based high-throughput screening method for engineering fatty acid synthases with improved production of medium-chain fatty acids
Xue, P., Si, T., Mishra, S., Zhang, L., Choe, K., Sweedler, J. V. & Zhao, H., Jul 1 2020, In: Biotechnology and bioengineering. 117, 7, p. 2131-2138 8 p.Research output: Contribution to journal › Article › peer-review
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An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms
Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In: Nature chemical biology. 16, 4, p. 387-390 4 p.Research output: Contribution to journal › Article › peer-review
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A New Biosensor for Stilbenes and a Cannabinoid Enabled by Genome Mining of a Transcriptional Regulator
Sun, H., Zhao, H. & Ang, E. L., Apr 17 2020, In: ACS synthetic biology. 9, 4, p. 698-705 8 p.Research output: Contribution to journal › Article › peer-review
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A New Era of Genome Integration - Simply Cut and Paste!
Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 601-609 9 p.Research output: Contribution to journal › Review article › peer-review
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A new fluorescence complementation biosensor for detection of estrogenic compounds
Mclachlan, M. J., Katzenellenbogen, J. A. & Zhao, H., Dec 2011, In: Biotechnology and bioengineering. 108, 12, p. 2794-2803 10 p.Research output: Contribution to journal › Article › peer-review
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An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine
Yin, J., Wei, Y., Liu, D., Hu, Y., Lu, Q., Lui Ang, E., Zhao, H. & Zhang, Y., Oct 25 2019, In: Journal of Biological Chemistry. 294, 43, p. 15662-15671 10 p.Research output: Contribution to journal › Article › peer-review
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A pathway for degradation of uracil to acetyl coenzyme a in bacillus megaterium
Zhu, D., Wei, Y., Yin, J., Liu, D., Ang, E. L., Zhao, H. & Zhang, Y., Apr 1 2020, In: Applied and environmental microbiology. 86, 7, e02837.Research output: Contribution to journal › Article › peer-review
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A pathway for isethionate dissimilation in Bacillus krulwichiae
Tong, Y., Wei, Y., Hu, Y., Ang, E. L., Zhao, H. & Zhang, Y., 2019, In: Applied and environmental microbiology. 85, 15, e00793-19.Research output: Contribution to journal › Article › peer-review
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A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae
Ren, H., Hu, P. & Zhao, H., Aug 2017, In: Biotechnology and bioengineering. 114, 8, p. 1847-1854 8 p.Research output: Contribution to journal › Article › peer-review
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Applying advanced dna assembly methods to generate pathway libraries
Eriksen, D. T., Chao, R. & Zhao, H., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 331-347 17 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter
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A Rewritable, Random-Access DNA-Based Storage System
Tabatabaei Yazdi, S. M. H., Yuan, Y., Ma, J., Zhao, H. & Milenkovic, O., Sep 18 2015, In: Scientific reports. 5, 14138.Research output: Contribution to journal › Article › peer-review
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Aryl-aldehyde formation in fungal polyketides: Discovery and characterization of a distinct biosynthetic mechanism
Wang, M., Beissner, M. & Zhao, H., Feb 20 2014, In: Chemistry and Biology. 21, 2, p. 257-263 7 p.Research output: Contribution to journal › Article › peer-review
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A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis
Xiong, X., Zhang, Y., Yan, J., Jain, S., Chee, S., Ren, B. & Zhao, H., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 1034-1042 9 p.Research output: Contribution to journal › Article › peer-review
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A single-chain TALEN architecture for genome engineering
Sun, N. & Zhao, H., Mar 1 2014, In: Molecular BioSystems. 10, 3, p. 446-453 8 p.Research output: Contribution to journal › Article › peer-review
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A step through the looking glass
Liang, Y., Ang, E. L. & Zhao, H., Sep 1 2016, In: Science China Life Sciences. 59, 9, p. 979-980 2 p.Research output: Contribution to journal › Article › peer-review
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A synthetic device for intracellular mRNA transport and local protein translation in eukaryotes
Abil, Z., Hoogenraad, C. & Zhao, H., 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, Vol. 1. p. 104-113 10 p.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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A transaldolase-dependent sulfoglycolysis pathway in Bacillus megaterium DSM 1804
Liu, Y., Wei, Y., Zhou, Y., Ang, E. L., Zhao, H. & Zhang, Y., Dec 17 2020, In: Biochemical and Biophysical Research Communications. 533, 4, p. 1109-1114 6 p.Research output: Contribution to journal › Article › peer-review
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A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases
Sun, N. & Zhao, H., 2014, Homing Endonucleases: Methods and Protocols. Humana Press Inc., p. 87-96 10 p. (Methods in Molecular Biology; vol. 1123).Research output: Chapter in Book/Report/Conference proceeding › Chapter
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A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency
Jeya, M., Kim, T. S., Kumar Tiwari, M., Li, J., Zhao, H. & Lee, J. K., Dec 2012, In: Molecular BioSystems. 8, 12, p. 3103-3106 4 p.Research output: Contribution to journal › Article › peer-review
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Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation
Lim, Y. H., Wong, F. T., Yeo, W. L., Ching, K. C., Lim, Y. W., Heng, E., Chen, S., Tsai, D. J., Lauderdale, T. L., Shia, K. S., Ho, Y. S., Hoon, S., Ang, E. L., Zhang, M. M. & Zhao, H., Aug 16 2018, In: ChemBioChem. 19, 16, p. 1716-1719 4 p.Research output: Contribution to journal › Article › peer-review
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Automated multiplex genome-scale engineering in yeast
Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., 2017, In: Nature communications. 8, 15187.Research output: Contribution to journal › Article › peer-review
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Biocatalysis for Drug Discovery and Development
Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., May 13 2016, Green Biocatalysis. Wiley, p. 421-455 35 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter
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Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates
Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In: Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.Research output: Contribution to journal › Review article › peer-review
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Biocatalyst development by directed evolution
Wang, M., Si, T. & Zhao, H., Jul 2012, In: Bioresource Technology. 115, p. 117-125 9 p.Research output: Contribution to journal › Article › peer-review
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Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca
Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Biochemical Journal. 476, 4, p. 733-746 14 p.Research output: Contribution to journal › Article › peer-review
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Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense
Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In: Biochemical Journal. 476, 11, p. 1605-1619 15 p.Research output: Contribution to journal › Article › peer-review
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Biosynthetic engineering of the antifungal, anti-MRSA auroramycin
Yeo, W. L., Heng, E., Tan, L. L., Lim, Y. W., Ching, K. C., Tsai, D. J., Jhang, Y. W., Lauderdale, T. L., Shia, K. S., Zhao, H., Ang, E. L., Zhang, M. M., Lim, Y. H. & Wong, F. T., Jan 6 2020, In: Microbial cell factories. 19, 1, 3.Research output: Contribution to journal › Article › peer-review
Open Access -
Biosystems design by directed evolution
Wang, Y., Yu, X. & Zhao, H., Mar 1 2020, In: AIChE Journal. 66, 3, e16716.Research output: Contribution to journal › Review article › peer-review
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Biosystems Design by Machine Learning
Volk, M. J., Lourentzou, I., Mishra, S., Vo, L. T., Zhai, C. & Zhao, H., Jul 17 2020, In: ACS synthetic biology. 9, 7, p. 1514-1533 20 p.Research output: Contribution to journal › Article › peer-review
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Breaking the silence: new strategies for discovering novel natural products
Ren, H., Wang, B. & Zhao, H., Dec 1 2017, In: Current Opinion in Biotechnology. 48, p. 21-27 7 p.Research output: Contribution to journal › Review article › peer-review
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Building a global alliance of biofoundries
Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, , Dec 1 2019, In: Nature communications. 10, 1, 2040.Research output: Contribution to journal › Comment/debate › peer-review
Open Access -
Building biological foundries for next-generation synthetic biology
Chao, R., Yuan, Y. B. & Zhao, H. M., Jul 24 2015, In: Science China Life Sciences. 58, 7, p. 658-665 8 p.Research output: Contribution to journal › Review article › peer-review
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Cell surface display of functional human MHC class II proteins: Yeast display versus insect cell display
Wen, F., Sethi, D. K., Wucherpfennig, K. W. & Zhao, H., Sep 2011, In: Protein Engineering, Design and Selection. 24, 9, p. 701-709 9 p.Research output: Contribution to journal › Article › peer-review
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Challenges and opportunities in synthetic biology for chemical engineers
Luo, Y., Lee, J. K. & Zhao, H., Nov 5 2013, In: Chemical Engineering Science. 103, p. 115-119 5 p.Research output: Contribution to journal › Article › peer-review
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Characterization and application of the Fe(ii) and α-ketoglutarate dependent hydroxylase FrbJ
Desieno, M. A., Van Der Donk, W. A. & Zhao, H., Sep 28 2011, In: Chemical Communications. 47, 36, p. 10025-10027 3 p.Research output: Contribution to journal › Article › peer-review