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• Drug Discovery and Development by Combinatorial Biosynthesis

  Desieno, M. A., Denard, C. A. & Zhao, H., May 25 2011, Enzyme Technologies: Metagenomics, Evolution, Biocatalysis, and Biosynthesis. John Wiley and Sons, p. 251-283 33 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Drug Discovery and Development via Synthetic Biology

  Cobb, R. E., Luo, Y., Freestone, T. & Zhao, H., Aug 27 2013, Synthetic Biology. Elsevier Inc., p. 183-206 24 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Synthetic biology
  • Synthetic Biology
  • Drug Discovery
  • Multigene Family
  • Biological Products

• Editorial: Synthetic biology and bioinformatics

  Medema, M. H. & Zhao, H., Aug 2016, In: Natural Product Reports. 33, 8, p. 913-914 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Editorial overview: Opportunities and challenges in synthetic biology

  Chang, M. C. Y. & Zhao, H., Oct 1 2015, In: Current Opinion in Chemical Biology. 28, p. 5-6 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Engineered biosynthesis of natural products in heterologous hosts

  Luo, Y., Li, B. Z., Liu, D., Zhang, L., Chen, Y., Jia, B., Zeng, B. X., Zhao, H. & Yuan, Y. J., Aug 7 2015, In: Chemical Society Reviews. 44, 15, p. 5265-5290 26 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthesis
  • Biological Products
  • Genes
  • Polyketides
  • Terpenes

• Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains

  Lian, J., Bao, Z., Hu, S. & Zhao, H., Jun 2018, In: Biotechnology and bioengineering. 115, 6, p. 1630-1635 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Polyploidy
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Genes
  • Yeasts

• Engineered minicellulosomes for consolidated bioprocessing

  Liang, Y., Ang, E. L. & Zhao, H., Jul 11 2016, Handbook of Carbohydrate-Modifying Biocatalysts. Pan Stanford Publishing Pte. Ltd., Vol. 2. p. 965-992 28 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae

  Liang, Y., Si, T., Ang, E. L. & Zhao, H., Jan 1 2014, In: Applied and environmental microbiology. 80, 21, p. 6677-6684 8 p.

  Research output: Contribution to journal › Article › peer-review

  • ethanol fermentation
  • saccharification
  • Fermentation
  • yeast
  • ethanol

• Engineering biological systems using automated biofoundries

  Chao, R., Mishra, S., Si, T. & Zhao, H., Jul 2017, In: Metabolic Engineering. 42, p. 98-108 11 p.

  Research output: Contribution to journal › Review article › peer-review

  • Microbial Genetics
  • Bioengineering
  • Biotechnology
  • Biological systems
  • Foundries

• Engineering chimeric RNA-binding proteins: Modular design, novel function

  Abil, Z., Denard, C. A., Hoogenraad, C. & Zhao, H., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 2).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Engineering microbial factories for synthesis of value-added products

  Du, J., Shao, Z. & Zhao, H., Aug 2011, In: Journal of Industrial Microbiology and Biotechnology. 38, 8, p. 873-890 18 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biofuels
  • Industrial plants
  • Alkaloids
  • Flavonoids
  • lignocellulose

• Engineering microbial hosts for production of bacterial natural products

  Zhang, M. M., Wang, Y., Ang, E. L. & Zhao, H., Aug 2016, In: Natural Product Reports. 33, 8, p. 963-987 25 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biological Products
  • Actinobacteria
  • Genetic Engineering
  • Genetic engineering
  • Fermentation

• Engineering novel tandem catalytic reactions using organometallic catalysts and metalloenzymes

  Denard, C. A., Huang, H., Lu, L., Bartlett, M. J., Hartwig, J. F. & Zhao, H., Jan 1 2013, Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

  Abil, Z. & Zhao, H., Jul 6 2015, In: Molecular BioSystems. 11, 10, p. 2658-2665 8 p.

  Research output: Contribution to journal › Article › peer-review

  • RNA-Binding Proteins
  • Transcriptome
  • Protein Engineering
  • RNA
  • RNA-Binding Motifs

• Engineering saccharomyces cerevisiae for biofuel production from lignocellulosic biomass

  Du, J. & Zhao, H., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Pentoses
  • Biofuels
  • Yeast
  • Biomass
  • Xylose

• Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production

  Wang, Y., Ren, H. & Zhao, H., Mar 4 2018, In: Critical Reviews in Biochemistry and Molecular Biology. 53, 2, p. 115-129 15 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biocatalysis
  • Enzymes
  • Metabolic engineering
  • Synthetic biology
  • Synthetic Biology

• Exploiting Issatchenkia orientalis SD108 for succinic acid production

  Xiao, H., Shao, Z., Jiang, Y., Dole, S. & Zhao, H., Aug 27 2014, In: Microbial cell factories. 13, 1, 121.

  Research output: Contribution to journal › Review article › peer-review

  • Organic acids
  • Succinic Acid
  • Acids
  • Yeast
  • Toxicity

• FairyTALE: A high-throughput TAL effector synthesis platform

  Liang, J., Chao, R., Abil, Z., Bao, Z. & Zhao, H., Feb 21 2014, In: ACS synthetic biology. 3, 2, p. 67-73 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effectors
  • Transcription
  • Throughput
  • DNA sequences
  • Transcription Activator-Like Effector Nucleases

• Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

  Yuan, Y., Andersen, E. & Zhao, H., Jan 15 2016, In: ACS synthetic biology. 5, 1, p. 46-52 7 p.

  Research output: Contribution to journal › Article › peer-review

  • DNA
  • Plasmids
  • Yeast
  • Ligases
  • Networks (circuits)

• Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry

  Chao, R., Liang, J., Tasan, I., Si, T., Ju, L. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 678-685 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Transcription
  • Foundries
  • Genes
  • DNA

• Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering

  Lian, J. & Zhao, H., Jul 15 2016, In: ACS synthetic biology. 5, 7, p. 689-697 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Oxidoreductases
  • Lipoylation
  • Yeast
  • Pyruvic Acid
  • Cytosol

• Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD)

  Tiwari, M. K., Lee, J. K., Moon, H. J. & Zhao, H., May 15 2011, In: Bioorganic and Medicinal Chemistry Letters. 21, 10, p. 2873-2876 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Dimerization
  • Mutagenesis
  • Oxygenases
  • Escherichia coli
  • Catalyst activity

• Genome-scale consequences of cofactor balancing in engineered pentose utilization pathways in Saccharomyces cerevisiae

  Ghosh, A., Zhao, H. & Price, N. D., Nov 4 2011, In: PloS one. 6, 11, e27316.

  Research output: Contribution to journal › Article › peer-review

  • Pentoses
  • Xylose
  • Yeast
  • Arabinose
  • pentoses

• Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

  Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J. & Zhao, H., Jul 1 2018, In: Nature Biotechnology. 36, 6, p. 505-508 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Nucleotides
  • Yeast
  • Saccharomyces cerevisiae
  • Genes
  • Genome

• Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes

  Tapsin, S., Sun, M., Shen, Y., Zhang, H., Lim, X. N., Susanto, T. T., Yang, S. L., Zeng, G. S., Lee, J., Lezhava, A., Ang, E. L., Zhang, L. H., Wang, Y., Zhao, H., Nagarajan, N. & Wan, Y., Dec 1 2018, In: Nature communications. 9, 1, 1289.

  Research output: Contribution to journal › Article › peer-review

  • prokaryotes
  • Flavin Mononucleotide
  • Nucleotide Aptamers
  • metabolites
  • genome

• Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

  Xiao, H. & Zhao, H., May 23 2014, In: Biotechnology for Biofuels. 7, 1, 78.

  Research output: Contribution to journal › Article › peer-review

  • Furaldehyde
  • SUMO-1 Protein
  • Furfural
  • RNA
  • Yeast

• High-Efficiency Genome Editing of Streptomyces Species by an Engineered CRISPR/Cas System

  Wang, Y., Cobb, R. E. & Zhao, H., 2016, Methods in Enzymology. O'Connor, S. E. (ed.). Academic Press Inc., p. 271-284 14 p. (Methods in Enzymology; vol. 575).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • CRISPR-Cas Systems
  • Gene Editing
  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Genes

• High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

  Cobb, R. E., Wang, Y. & Zhao, H., Jun 19 2015, In: ACS synthetic biology. 4, 6, p. 723-728 6 p.

  Research output: Contribution to journal › Article › peer-review

  • CRISPR-Cas Systems
  • Gene Editing
  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Genes

• Highlights of biocatalysis and biomimetic catalysis

  Zhao, H., Sep 2 2011, In: ACS Catalysis. 1, 9, p. 1119-1120 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Highly Efficient Single-Pot Scarless Golden Gate Assembly

  Hamedirad, M., Weisberg, S., Chao, R., Lian, J. & Zhao, H., May 17 2019, In: ACS synthetic biology. 8, 5, p. 1047-1054 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Software Design
  • Point Mutation
  • Cicatrix
  • DNA
  • Software design

• High throughput screening and selection methods for directed enzyme evolution

  Xiao, H., Bao, Z. & Zhao, H., Apr 29 2015, In: Industrial and Engineering Chemistry Research. 54, 16, p. 4011-4020 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Enzymes
  • Screening
  • Throughput
  • Costs

• High-throughput screening or selection methods for evolutionary enzyme engineering

  Shi, S., Zhang, H., Ang, E. L. & Zhao, H., May 17 2016, Understanding Enzymes: Function, Design, Engineering and Analysis. Pan Stanford Publishing Pte. Ltd., p. 707-744 38 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Homology-integrated CRISPR-cas (HI-CRISPR) system for one-step multigene disruption in saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, p. 27-36 10 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 1).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • gene targeting
  • Saccharomyces cerevisiae
  • Genes

• Homology-integrated CRISPR-cas (HI-CRISPR) system for one-step multigene disruption in saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., Jan 1 2014, Systems Biology 2014 - Topical Conference at the 2014 AIChE Annual Meeting. AIChE, p. 3-12 10 p. (Systems Biology 2014 - Topical Conference at the 2014 AIChE Annual Meeting).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • Genes
  • Biosynthetic Pathways

• Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., May 15 2015, In: ACS synthetic biology. 4, 5, p. 585-594 10 p.

  Research output: Contribution to journal › Article › peer-review

  • CRISPR-Associated Proteins
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • Proteins

• Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense

  Zhou, Y., Wei, Y., Nanjaraj Urs, A. N., Lin, L., Xu, T., Hu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Bioscience Reports. 39, 6, Y.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • sulfoacetaldehyde
  • Bifidobacterium
  • Taurine
  • Bacteria
  • Oxidoreductases

• Identification and engineering of n-butanol biosensors by transcriptome analysis in yeast

  Shi, S., Ang, E. L., Tan, M. H. & Zhao, H., Jan 1 2016, Metabolic Engineering 11. AIChE, p. 458-459 2 p. (Metabolic Engineering 11).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Identification of an important motif that controls the activity and specificity of sugar transporters

  Wang, M., Yu, C. & Zhao, H., Jul 1 2016, In: Biotechnology and bioengineering. 113, 7, p. 1460-1467 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Sugars
  • Glucose
  • xylose-glucose
  • Facilitative Glucose Transport Proteins

• Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB)

  Zhao, H., Jan 1 2015, Synthetic Biology: Engineering, Evolution, and Design Conference 2015, SEED 2015. AIChE, p. 784-785 2 p. (Synthetic Biology: Engineering, Evolution, and Design Conference 2015, SEED 2015; vol. 2).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Improving and repurposing biocatalysts via directed evolution

  Denard, C. A., Ren, H. & Zhao, H., Apr 1 2015, In: Current Opinion in Chemical Biology. 25, p. 55-64 10 p.

  Research output: Contribution to journal › Review article › peer-review

  • Enzymes
  • Stereoselectivity
  • Biocatalysis
  • Chemical reactivity
  • Protein Engineering

• Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

  Wen, S., Tan, T. & Zhao, H., Dec 5 2012, In: Journal of Biotechnology. 164, 2, p. 248-253 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Yarrowia
  • Lipases
  • Lipase
  • Enzymes
  • Half-Life

• Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

  Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A. N., Liu, Y., Ang, E. L., Zhao, S., Zhao, H. & Zhang, Y., Dec 1 2018, In: Nature communications. 9, 1, 4224.

  Research output: Contribution to journal › Article › peer-review

  • Skatole
  • indoleacetic acid
  • fermentation
  • Carboxy-Lyases
  • tryptophan

• Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

  Abil, Z., Gumy, L. F., Zhao, H. & Hoogenraad, C. C., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 950-956 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Carrier Proteins
  • Messenger RNA
  • RNA
  • RNA-Binding Proteins
  • Ligands

• Industrial biotechnology: Tools and applications

  Zhao, H. & Tan, T., Nov 15 2015, In: Biotechnology Advances. 33, 7, p. 1393-1394 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Insights into Cell-Free Conversion of CO₂ to Chemicals by a Multienzyme Cascade Reaction

  Singh, R. K., Singh, R., Sivakumar, D., Kondaveeti, S., Kim, T., Li, J., Sung, B. H., Cho, B. K., Kim, D. R., Kim, S. C., Kalia, V. C., Zhang, Y. H. P. J., Zhao, H., Kang, Y. C. & Lee, J. K., Dec 7 2018, In: ACS Catalysis. 8, 12, p. 11085-11093 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Oxidoreductases
  • NAD phosphite oxidoreductase
  • Methanol
  • glutathione-independent formaldehyde dehydrogenase
  • Formaldehyde

• In Situ Assembly of Antifouling/Bacterial Silver Nanoparticle-Hydrogel Composites with Controlled Particle Release and Matrix Softening

  Baek, K., Liang, J., Lim, W. T., Zhao, H., Kim, D. H. & Kong, H., Jul 22 2015, In: ACS Applied Materials and Interfaces. 7, 28, p. 15359-15367 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Silver nanoparticles
  • Hydrogels
  • Polyethylene Glycol Dimethacrylate Hydrogel
  • Gels
  • Composite materials

• Introduction

  Zhao, H., Aug 27 2013, In: Synthetic Biology.

  Research output: Contribution to journal › Article › peer-review

• Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via ¹³C metabolic flux analysis

  Feng, X. & Zhao, H., Sep 1 2013, In: AIChE Journal. 59, 9, p. 3195-3202 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Flux Analysis
  • xylose-glucose
  • Xylose
  • Metabolism
  • Yeast

• Investigating host dependence of xylose utilization in recombinant Saccharomyces cerevisiae strains using RNA-seq analysis

  Feng, X. & Zhao, H., 2013, In: Biotechnology for Biofuels. 6, 1, 96.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • RNA
  • Yeast
  • Saccharomyces cerevisiae
  • regulation

• Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via ¹³C metabolic flux analysis

  Feng, X. & Zhao, H., Nov 18 2013, In: Microbial cell factories. 12, 1, 114.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Flux Analysis
  • Xylose
  • Metabolism
  • Yeast
  • Saccharomyces cerevisiae