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2012

Proton transfer in ba 3 cytochrome c oxidase from Thermus thermophilus

Von Ballmoos, C., Ädelroth, P., Gennis, R. B. & Brzezinski, P., Apr 1 2012, In : Biochimica et Biophysica Acta - Bioenergetics. 1817, 4, p. 650-657 8 p.

Research output: Contribution to journalReview article

Thermus thermophilus
Proton transfer
Electron Transport Complex IV
Protons
Oxidoreductases
2013

Characterization of the nitric oxide reductase from Thermus thermophilus

Schurig-Briccio, L. A., Venkatakrishnan, P., Hemp, J., Bricio, C., Berenguer, J. & Gennis, R. B., Jul 30 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 31, p. 12613-12618 6 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Heme
Catalytic Domain
Oxidoreductases
Enzymes
2010
Bacteriorhodopsins
Deprotonation
Proton transfer
Protons
Water
2012

Characterization of the PIB-Type ATPases present in Thermus thermophilus

Schurig-Briccio, L. A. & Gennis, R. B., Aug 1 2012, In : Journal of bacteriology. 194, 15, p. 4107-4113 7 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Adenosine Triphosphatases
Copper
Genes
Cadmium
2014

Subunit CydX of Escherichia coli cytochrome bd ubiquinol oxidase is essential for assembly and stability of the di-heme active site

Hoeser, J., Hong, S., Gehmann, G., Gennis, R. B. & Friedrich, T., May 2 2014, In : FEBS Letters. 588, 9, p. 1537-1541 5 p.

Research output: Contribution to journalArticle

Cytochromes
Heme
Escherichia coli
Catalytic Domain
Technology
2016

A Purple Cupredoxin from Nitrosopumilus maritimus Containing a Mononuclear Type 1 Copper Center with an Open Binding Site

Hosseinzadeh, P., Tian, S., Marshall, N. M., Hemp, J., Mullen, T., Nilges, M. J., Gao, Y. G., Robinson, H., Stahl, D. A., Gennis, R. B. & Lu, Y., May 25 2016, In : Journal of the American Chemical Society. 138, 20, p. 6324-6327 4 p.

Research output: Contribution to journalArticle

Binding sites
Copper
Binding Sites
electron spin resonance
copper
2015

Antiinfectives targeting enzymes and the proton motive force

Feng, X., Zhu, W., Schurig-Briccio, L. A., Lindert, S., Shoen, C., Hitchings, R., Li, J., Wang, Y., Baig, N., Zhou, T., Kim, B. K., Crick, D. C., Cynamon, M., McCammon, J. A., Gennis, R. B. & Oldfield, E., Dec 22 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 51, p. E7073-E7082

Research output: Contribution to journalArticle

Proton-Motive Force
Mycobacterium tuberculosis
undecaprenyl pyrophosphate synthetase
Membranes
Staphylococcus aureus
2012

Functional importance of a pair of conserved glutamic acid residues and of Ca2+ binding in the cbb 3-type oxygen reductases from rhodobacter sphaeroides and vibrio cholerae

Ouyang, H., Han, H., Roh, J. H., Hemp, J., Hosler, J. P. & Gennis, R. B., Sep 18 2012, In : Biochemistry. 51, 37, p. 7290-7296 7 p.

Research output: Contribution to journalArticle

Rhodobacter sphaeroides
Vibrio cholerae
Heme
Glutamic Acid
Glutamates
2010

Nitroxide spin labels as EPR reporters of the relaxation and magnetic properties of the heme-copper site in cytochrome bo 3, E. coli

Oganesyan, V. S., White, G. F., Field, S., Marritt, S., Gennis, R. B., Yap, L. L. & Thomson, A. J., Nov 1 2010, In : Journal of Biological Inorganic Chemistry. 15, 8, p. 1255-1264 10 p.

Research output: Contribution to journalArticle

Spin Labels
Heme
Relaxation time
Paramagnetic resonance
Copper

Catalase activity of cytochrome c oxidase assayed with hydrogen peroxide-sensitive electrode microsensor

Bolshakov, I. A., Vygodina, T. V., Gennis, R. B., Karyakin, A. A. & Konstantinov, A. A., Nov 1 2010, In : Biochemistry (Moscow). 75, 11, p. 1352-1360 9 p.

Research output: Contribution to journalArticle

Microsensors
Electron Transport Complex IV
Catalase
Hydrogen Peroxide
Electrodes
2013

Proton uptake and pKa changes in the uncoupled Asn139Cys variant of cytochrome c oxidase

Johansson, A. L., Carlsson, J., Högbom, M., Hosler, J. P., Gennis, R. B. & Brzezinski, P., Feb 5 2013, In : Biochemistry. 52, 5, p. 827-836 10 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Protons
Membrane Proteins
Membranes
Proton Pumps
2019

Single-particle cryo-EM studies of transmembrane proteins in SMA copolymer nanodiscs

Sun, C. & Gennis, R. B., Jul 2019, In : Chemistry and Physics of Lipids. 221, p. 114-119 6 p.

Research output: Contribution to journalReview article

Styrene
Membrane Proteins
Copolymers
Lipids
Proteins
2010

The diheme cytochrome c 4 from Vibrio cholerae is a natural electron donor to the respiratory cbb 3 oxygen reductase

Chang, H. Y., Ahn, Y., Pace, L. A., Lin, M. T., Lin, Y. H. & Gennis, R. B., Sep 7 2010, In : Biochemistry. 49, 35, p. 7494-7503 10 p.

Research output: Contribution to journalArticle

Vibrio cholerae
Oxidoreductases
Electrons
Oxygen
Proteobacteria

Modeling direct electron transfer to a multi-redox center protein: Cytochrome c Oxidase

Schach, D., Nowak, C., Gennis, R. B., Ferguson-Miller, S., Knoll, W., Walz, D. & Naumann, R. L. C., Nov 15 2010, In : Journal of Electroanalytical Chemistry. 649, 1-2, p. 268-276 9 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Proteins
Electrons
Lipid bilayers
Enzymes
2012

Spectral identification of intermediates generated during the reaction of dioxygen with the wild-type and EQ(I-286) mutant of rhodobacter sphaeroides cytochrome c oxidase

Szundi, I., Funatogawa, C., Cassano, J., McDonald, W., Ray, J., Hiser, C., Ferguson-Miller, S., Gennis, R. B. & Einarsdóttir, Ó., Nov 20 2012, In : Biochemistry. 51, 46, p. 9302-9311 10 p.

Research output: Contribution to journalArticle

Rhodobacter sphaeroides
Electron Transport Complex IV
Oxygen
Enzymes
Protons

Reconstitution of respiratory oxidases in membrane nanodiscs for investigation of proton-coupled electron transfer

Näsvik Öjemyr, L., Von Ballmoos, C., Gennis, R. B., Sligar, S. & Brzezinski, P., Mar 9 2012, In : FEBS Letters. 586, 5, p. 640-645 6 p.

Research output: Contribution to journalReview article

Liposomes
Protons
Oxidoreductases
Electrons
Membranes
2018

Cytochromes bd-I and bo3 are essential for the bactericidal effect of microcin J25 on Escherichia coli cells

Galván, A. E., Chalón, M. C., Schurig-Briccio, L. A., Salomón, R. A., Minahk, C. J., Gennis, R. B. & Bellomio, A., Feb 2018, In : Biochimica et Biophysica Acta - Bioenergetics. 1859, 2, p. 110-118 9 p.

Research output: Contribution to journalArticle

Open Access
Cytochromes
Escherichia coli
Cell Respiration
Oxygen
Membranes
2013

Characterization of the Type III sulfide:quinone oxidoreductase from Caldivirga maquilingensis and its membrane binding

Lencina, A. M., Ding, Z., Schurig-Briccio, L. A. & Gennis, R. B., Mar 1 2013, In : Biochimica et Biophysica Acta - Bioenergetics. 1827, 3, p. 266-275 10 p.

Research output: Contribution to journalArticle

sulfide quinone reductase
Membranes
Sulfides
Enzymes
Electrons
2017

X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization

Schieferstein, J. M., Pawate, A. S., Sun, C., Wan, F., Sheraden, P. N., Broecker, J., Ernst, O. P., Gennis, R. B. & Kenis, P. J. A., Mar 1 2017, In : Biomicrofluidics. 11, 2, 024118.

Research output: Contribution to journalArticle

Microfluidics
Crystallization
Screening
Membrane Proteins
screening
2015

Plasticity in the High Affinity Menaquinone Binding Site of the Cytochrome aa3-600 Menaquinol Oxidase from Bacillus subtilis

Yi, S. M., Taguchi, A. T., Samoilova, R. I., O'Malley, P. J., Gennis, R. B. & Dikanov, S. A., Jul 21 2015, In : Biochemistry. 54, 32, p. 5030-5044 15 p.

Research output: Contribution to journalArticle

Vitamin K 2
Electron Transport Complex IV
Bacilli
Bacillus subtilis
Plasticity
2016

The CO Photodissociation and Recombination Dynamics of the W172Y/F282T Ligand Channel Mutant of Rhodobacter sphaeroides aa3 Cytochrome c Oxidase

Cassano, J. A., Choi, S. K., McDonald, W., Szundi, I., Villa Gawboy, T. R., Gennis, R. B. & Einarsdóttir, Ó., May 1 2016, In : Photochemistry and Photobiology. 92, 3, p. 410-419 10 p.

Research output: Contribution to journalArticle

Photodissociation
Rhodobacter sphaeroides
oxidase
cytochromes
Electron Transport Complex IV
2010

Partial steps of charge translocation in the nonpumping N139l mutant of rhodobacter sphaeroides cytochrome c oxidase with a blocked d-channel

Siletsky, S. A., Zhu, J., Gennis, R. B. & Konstantinov, A. A., Apr 13 2010, In : Biochemistry. 49, 14, p. 3060-3073 14 p.

Research output: Contribution to journalArticle

Rhodobacter sphaeroides
Electron Transport Complex IV
Protons
Enzymes
Oxidoreductases
2018

Unpaired Electron Spin Density Distribution across Reduced [2Fe-2S] Cluster Ligands by 13Cβ-Cysteine Labeling

Taguchi, A. T., Miyajima-Nakano, Y., Fukazawa, R., Lin, M. T., Baldansuren, A., Gennis, R. B., Hasegawa, K., Kumasaka, T., Dikanov, S. & Iwasaki, T., Jan 16 2018, In : Inorganic Chemistry. 57, 2, p. 741-746 6 p.

Research output: Contribution to journalArticle

cysteine
electron spin
Labeling
marking
Cysteine
2012

Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal rieske-type ferredoxin

Iwasaki, T., Fukazawa, R., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M. T., Gennis, R. B., Hasegawa, K., Kumasaka, T. & Dikanov, S., Dec 5 2012, In : Journal of the American Chemical Society. 134, 48, p. 19731-19738 8 p.

Research output: Contribution to journalArticle

Isotope Labeling
Ferredoxins
Dissection
dissection
Sulfur
2014

Multitarget drug discovery for tuberculosis and other infectious diseases

Li, K., Schurig-Briccio, L. A., Feng, X., Upadhyay, A., Pujari, V., Lechartier, B., Fontes, F. L., Yang, H., Rao, G., Zhu, W., Gulati, A., No, J. H., Cintra, G., Bogue, S., Liu, Y. L., Molohon, K., Orlean, P., Mitchell, D. A., Freitas-Junior, L., Ren, F. & 8 others, Sun, H., Jiang, T., Li, Y., Guo, R. T., Cole, S. T., Gennis, R. B., Crick, D. C. & Oldfield, E., Apr 10 2014, In : Journal of Medicinal Chemistry. 57, 7, p. 3126-3129 4 p.

Research output: Contribution to journalReview article

Drug Discovery
Malaria
Communicable Diseases
Parasites
Tuberculosis
2019

Active site rearrangement and structural divergence in prokaryotic respiratory oxidases

Safarian, S., Hahn, A., Mills, D. J., Radloff, M., Eisinger, M. L., Nikolaev, A., Meier-Credo, J., Melin, F., Miyoshi, H., Gennis, R. B., Sakamoto, J., Langer, J. D., Hellwig, P., Kühlbrandt, W. & Michel, H., Oct 4 2019, In : Science. 366, 6461, p. 100-104 5 p.

Research output: Contribution to journalArticle

Catalytic Domain
Oxidoreductases
Oxygen
Electron Transport Complex IV
Cytochromes
2014

A third subunit in ancestral cytochrome c-dependent nitric oxide reductases

Bricio, C., Alvarez, L., San Martin, M., Schurig-Briccio, L. A., Gennis, R. B. & Berenguer, J., Aug 2014, In : Applied and environmental microbiology. 80, 16, p. 4871-4878 8 p.

Research output: Contribution to journalArticle

cytochrome c
nitric oxide
Cytochromes c
cytochrome
Thermus thermophilus
2010

Functional interactions between membrane-bound transporters and membranes

Öjemyr, L. N., Lee, H. J., Gennis, R. B. & Brzezinski, P., Sep 7 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 36, p. 15763-15767 5 p.

Research output: Contribution to journalArticle

Membrane Transport Proteins
Membranes
Protons
Membrane Proteins
Respiratory Transport

Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba 3 cytochrome c oxidase from thermus thermophilus

Smirnova, I., Reimann, J., Von Ballmoos, C., Chang, H. Y., Gennis, R. B., Fee, J. A., Brzezinski, P. & Ädelroth, P., Aug 24 2010, In : Biochemistry. 49, 33, p. 7033-7039 7 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Proton transfer
Electron Transport Complex IV
Protons
Oxidoreductases
2018

Type 2 NADH dehydrogenase is the only point of entry for electrons into the Streptococcus agalactiae respiratory chain and is a potential drug target

Lencina, A. M., Franza, T., Sullivan, M. J., Ulett, G. C., Ipe, D. S., Gaudu, P., Gennis, R. B. & Schurig-Briccio, L. A., Jul 1 2018, In : mBio. 9, 4, e01034-18.

Research output: Contribution to journalArticle

Open Access
NADH Dehydrogenase
Streptococcus agalactiae
Electron Transport
Electrons
Oxidoreductases
Oxidoreductases
Nitric Oxide
Substrates
Catalytic Domain
Tunnels
2016
Thermus thermophilus
Lipid bilayers
Lipid Bilayers
Catalytic Domain
Tunnels
2013

Solid-state NMR study of a 41 kDa membrane protein complex DsbA/DsbB

Sperling, L. J., Tang, M., Berthold, D. A., Nesbitt, A. E., Gennis, R. B. & Rienstra, C. M., May 23 2013, In : Journal of Physical Chemistry B. 117, 20, p. 6052-6060 9 p.

Research output: Contribution to journalArticle

Chemical shift
chemical equilibrium
Catalytic Domain
Membrane Proteins
Nuclear magnetic resonance

Direct electrochemistry of cytochrome bo3 oxidase at a series of gold nanoparticles-modified electrodes

Melin, F., Meyer, T., Lankiang, S., Choi, S. K., Gennis, R. B., Blanck, C., Schmutz, M. & Hellwig, P., Jan 1 2013, In : Electrochemistry Communications. 26, 1, p. 105-108 4 p.

Research output: Contribution to journalArticle

Electrochemistry
Gold
Nanoparticles
Proteins
Immobilized Enzymes
2012
Rhodobacter sphaeroides
Electron Transport Complex IV
Cytochromes c
Horses
His-His-His-His-His-His
2017

Location of the Substrate Binding Site of the Cytochrome bo3 Ubiquinol Oxidase from Escherichia coli

Choi, S. K., Schurig-Briccio, L., Ding, Z., Hong, S., Sun, C. & Gennis, R. B., Jun 21 2017, In : Journal of the American Chemical Society. 139, 24, p. 8346-8354 9 p.

Research output: Contribution to journalArticle

Binding sites
Cytochromes
Escherichia coli
cytochrome
coenzyme Q10

Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli

Choi, S. K., Lin, M. T., Ouyang, H. & Gennis, R. B., May 1 2017, In : Biochimica et Biophysica Acta - Bioenergetics. 1858, 5, p. 366-370 5 p.

Research output: Contribution to journalArticle

Ubiquinone
Cytochromes
Escherichia coli
Binding Sites
Heme
2010

A positive feedback-based gene circuit to increase the production of a membrane protein

Bansal, K., Yang, K., Nistala, G. J., Gennis, R. B. & Bhalerao, K. D., May 25 2010, In : Journal of Biological Engineering. 4, 6.

Research output: Contribution to journalArticle

Gene Regulatory Networks
Membrane Proteins
Genes
Proteins
Membranes
2015

Division of labor in transhydrogenase by alternating proton translocation and hydride transfer

Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B. & Stout, C. D., Jan 9 2015, In : Science. 347, 6218, p. 178-181 4 p.

Research output: Contribution to journalArticle

NADP
Protons
NADP Transhydrogenases
Thermus thermophilus
Proton-Motive Force
2018

X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds

Schieferstein, J. M., Pawate, A. S., Varel, M. J., Guha, S., Astrauskaite, I., Gennis, R. B. & Kenis, P. J. A., Jan 1 2018, In : Lab on a chip. 18, 6, p. 944-954 11 p.

Research output: Contribution to journalArticle

Microfluidics
Crystallization
Membrane Proteins
X-Rays
Proteins
2012

Exploring the proton pump and exit pathway for pumped protons in cytochrome ba 3 from Thermus thermophilus

Chang, H. Y., Choi, S. K., Vakkasoglu, A. S., Chen, Y., Hemp, J., Fee, J. A. & Gennis, R. B., Apr 3 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 14, p. 5259-5264 6 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Proton Pumps
Propionates
Protons
Hydrogen
2014

Conformational coupling between the active site and residues within the KC-channel of the Vibrio cholerae cbb3-type (C-family) oxygen reductase

Ahn, Y. O., Mahinthichaichan, P., Lee, H. J., Ouyang, H., Kaluka, D., Yeh, S. R., Arjona, D., Rousseau, D. L., Tajkhorshid, E., Ädelroth, P. & Gennis, R. B., Oct 21 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 42, p. E4419-E4428

Research output: Contribution to journalArticle

Vibrio cholerae
Catalytic Domain
Oxidoreductases
Oxygen
Protons

The KC channel in the cbb3-type respiratory oxygen reductase from Rhodobacter capsulatus is required for both chemical and pumped protons

Yildiz, G. G., Gennis, R. B., Daldal, F. & Öztürk, M., 2014, In : Journal of bacteriology. 196, 10, p. 1825-1832 8 p.

Research output: Contribution to journalArticle

Rhodobacter capsulatus
Protons
Oxidoreductases
Oxygen
Heme
2010

A pathogenic mutation in cytochrome c oxidase results in impaired proton pumping while retaining O2-reduction activity

Namslauer, I., Lee, H. J., Gennis, R. B. & Brzezinski, P., May 1 2010, In : Biochimica et Biophysica Acta - Bioenergetics. 1797, 5, p. 550-556 7 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Proton transfer
Protons
Mutation
Substitution reactions
2015

Mutation of a single residue in the ba3 oxidase specifically impairs protonation of the pump site

Von Ballmoos, C., Gonska, N., Lachmann, P., Gennis, R. B., Ädelroth, P. & Brzezinski, P., Mar 17 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 11, p. 3397-3402 6 p.

Research output: Contribution to journalArticle

Protons
Oxidoreductases
Mutation
Electron Transport Complex IV
Electrons
2018

The electron distribution in the "activated" state of cytochrome c oxidase

Vilhjálmsdóttir, J., Gennis, R. B. & Brzezinski, P., Dec 1 2018, In : Scientific reports. 8, 1, 7502.

Research output: Contribution to journalArticle

Open Access
Electron Transport Complex IV
Electrons
Protons
Free energy
Membranes
2013

Single mutations that redirect internal proton transfer in the ba 3 oxidase from thermus thermophilus

Smirnova, I., Chang, H. Y., Von Ballmoos, C., Ädelroth, P., Gennis, R. B. & Brzezinski, P., Oct 8 2013, In : Biochemistry. 52, 40, p. 7022-7030 9 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Proton transfer
Protons
Oxidoreductases
Mutation
2014

Kinetics and intermediates of the reaction of fully reduced escherichia coli bo 3 ubiquinol oxidase with O2

Szundi, I., Kittredge, C., Choi, S. K., McDonald, W., Ray, J., Gennis, R. B. & Einarsdóttir, Ó., Jan 1 2014, In : Biochemistry. 53, 33, p. 5393-5404 12 p.

Research output: Contribution to journalArticle

Escherichia coli
Heme
Kinetics
Cytochromes
Oxidation
2015

Review and Hypothesis. New insights into the reaction mechanism of transhydrogenase: Swivelling the dIII component may gate the proton channel

Jackson, J. B., Leung, J. H., Stout, C. D., Schurig-Briccio, L. A. & Gennis, R. B., Jul 20 2015, In : FEBS Letters. 589, 16, p. 2027-2033 7 p.

Research output: Contribution to journalReview article

NADP
Protons
Thermus thermophilus
Mitochondria
Enzymes