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2010

Modeling direct electron transfer to a multi-redox center protein: Cytochrome c Oxidase

Schach, D., Nowak, C., Gennis, R. B., Ferguson-Miller, S., Knoll, W., Walz, D. & Naumann, R. L. C., Nov 15 2010, In : Journal of Electroanalytical Chemistry. 649, 1-2, p. 268-276 9 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Proteins
Electrons
Lipid bilayers
Enzymes
2013

Characterization of the Type III sulfide:quinone oxidoreductase from Caldivirga maquilingensis and its membrane binding

Lencina, A. M., Ding, Z., Schurig-Briccio, L. A. & Gennis, R. B., Mar 1 2013, In : Biochimica et Biophysica Acta - Bioenergetics. 1827, 3, p. 266-275 10 p.

Research output: Contribution to journalArticle

sulfide quinone reductase
Membranes
Sulfides
Enzymes
Electrons
2010

Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba 3 cytochrome c oxidase from thermus thermophilus

Smirnova, I., Reimann, J., Von Ballmoos, C., Chang, H. Y., Gennis, R. B., Fee, J. A., Brzezinski, P. & Ädelroth, P., Aug 24 2010, In : Biochemistry. 49, 33, p. 7033-7039 7 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Proton transfer
Electron Transport Complex IV
Protons
Oxidoreductases
2017

X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization

Schieferstein, J. M., Pawate, A. S., Sun, C., Wan, F., Sheraden, P. N., Broecker, J., Ernst, O. P., Gennis, R. B. & Kenis, P. J. A., Mar 1 2017, In : Biomicrofluidics. 11, 2, 024118.

Research output: Contribution to journalArticle

Microfluidics
Crystallization
Screening
Membrane Proteins
screening
2014

A third subunit in ancestral cytochrome c-dependent nitric oxide reductases

Bricio, C., Alvarez, L., San Martin, M., Schurig-Briccio, L. A., Gennis, R. B. & Berenguer, J., Aug 2014, In : Applied and environmental microbiology. 80, 16, p. 4871-4878 8 p.

Research output: Contribution to journalArticle

cytochrome c
nitric oxide
Cytochromes c
cytochrome
Thermus thermophilus
2010

Partial steps of charge translocation in the nonpumping N139l mutant of rhodobacter sphaeroides cytochrome c oxidase with a blocked d-channel

Siletsky, S. A., Zhu, J., Gennis, R. B. & Konstantinov, A. A., Apr 13 2010, In : Biochemistry. 49, 14, p. 3060-3073 14 p.

Research output: Contribution to journalArticle

Rhodobacter sphaeroides
Electron Transport Complex IV
Protons
Enzymes
Oxidoreductases
2016
Thermus thermophilus
Lipid bilayers
Lipid Bilayers
Catalytic Domain
Tunnels

The CO Photodissociation and Recombination Dynamics of the W172Y/F282T Ligand Channel Mutant of Rhodobacter sphaeroides aa3 Cytochrome c Oxidase

Cassano, J. A., Choi, S. K., McDonald, W., Szundi, I., Villa Gawboy, T. R., Gennis, R. B. & Einarsdóttir, Ó., May 1 2016, In : Photochemistry and Photobiology. 92, 3, p. 410-419 10 p.

Research output: Contribution to journalArticle

Photodissociation
Rhodobacter sphaeroides
oxidase
cytochromes
Electron Transport Complex IV
2011

2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer

Nowak, C., Laredo, T., Gebert, J., Lipkowski, J., Gennis, R. B., Ferguson-Miller, S., Knoll, W. & Naumann, R. L. C., Jun 1 2011, In : Metallomics. 3, 6, p. 619-627 9 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Spectrum Analysis
Spectroscopy
Electrons
Biomimetics
2013

Direct electrochemistry of cytochrome bo3 oxidase at a series of gold nanoparticles-modified electrodes

Melin, F., Meyer, T., Lankiang, S., Choi, S. K., Gennis, R. B., Blanck, C., Schmutz, M. & Hellwig, P., Jan 1 2013, In : Electrochemistry Communications. 26, 1, p. 105-108 4 p.

Research output: Contribution to journalArticle

Electrochemistry
Gold
Nanoparticles
Proteins
Immobilized Enzymes
2014

The semiquinone at the Qisite of the bc 1complex explored using HYSCORE spectroscopy and specific isotopic labeling of ubiquinone in rhodobacter sphaeroides via 13c methionine and construction of a methionine auxotroph

Hong, S., De Almeida, W. B., Taguchi, A. T., Samoilova, R. I., Gennis, R. B., O'Malley, P. J., Dikanov, S. & Crofts, A. R., Sep 30 2014, In : Biochemistry. 53, 38, p. 6022-6031 10 p.

Research output: Contribution to journalArticle

Rhodobacter sphaeroides
Ubiquinone
Methionine
Labeling
Spectrum Analysis
2018
Oxidoreductases
Nitric Oxide
Substrates
Catalytic Domain
Tunnels
2011

Microfluidic platforms for in situ crystallographic analysis

Perry, S. L., Guha, S., Pawate, A., Gennis, R. B., Ren, Z. & Kenis, P. J. A., Jan 1 2011, Separations Division - Core Programming Topic at the 2011 AIChE Annual Meeting. AIChE, p. 602-603 2 p. (Separations Division - Core Programming Topic at the 2011 AIChE Annual Meeting; vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Microfluidics
2010

A positive feedback-based gene circuit to increase the production of a membrane protein

Bansal, K., Yang, K., Nistala, G. J., Gennis, R. B. & Bhalerao, K. D., May 25 2010, In : Journal of Biological Engineering. 4, 6.

Research output: Contribution to journalArticle

Gene Regulatory Networks
Membrane Proteins
Genes
Proteins
Membranes
2015

Mutation of a single residue in the ba3 oxidase specifically impairs protonation of the pump site

Von Ballmoos, C., Gonska, N., Lachmann, P., Gennis, R. B., Ädelroth, P. & Brzezinski, P., Mar 17 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 11, p. 3397-3402 6 p.

Research output: Contribution to journalArticle

Protons
Oxidoreductases
Mutation
Electron Transport Complex IV
Electrons
2013

Characterization of the nitric oxide reductase from Thermus thermophilus

Schurig-Briccio, L. A., Venkatakrishnan, P., Hemp, J., Bricio, C., Berenguer, J. & Gennis, R. B., Jul 30 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 31, p. 12613-12618 6 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Heme
Catalytic Domain
Oxidoreductases
Enzymes
2011

Entrance of the proton pathway in cbb 3-type heme-copper oxidases

Lee, H. J., Gennis, R. B. & Ädelroth, P., Oct 25 2011, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 43, p. 17661-17666 6 p.

Research output: Contribution to journalArticle

Heme
Protons
Rhodobacter sphaeroides
Proton-Motive Force
Membranes
2015

The two transmembrane helices of CcoP are sufficient for assembly of the cbb3-type heme-copper oxygen reductase from Vibrio cholerae

Ahn, Y. O., Lee, H. J., Kaluka, D., Yeh, S. R., Rousseau, D. L., Ädelroth, P. & Gennis, R. B., Jul 13 2015, In : Biochimica et Biophysica Acta - Bioenergetics. 1847, 10, p. 1231-1239 9 p.

Research output: Contribution to journalArticle

Vibrio cholerae
Heme
Copper
Oxidoreductases
Oxygen
2010

Characterization of the semiquinone radical stabilized by the cytochrome aa3-600 menaquinol oxidase of Bacillus subtilis

Yi, S. M., Narasimhulu, K. V., Samoilova, R. I., Gennis, R. B. & Dikanov, S. A., Jun 11 2010, In : Journal of Biological Chemistry. 285, 24, p. 18241-18251 11 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Bacilli
Cytochromes
Bacillus subtilis
Ubiquinone
2014

Characterization of the type 2 NADH:menaquinone oxidoreductases from Staphylococcus aureus and the bactericidal action of phenothiazines

Schurig-Briccio, L. A., Yano, T., Rubin, H. & Gennis, R. B., Jul 2014, In : Biochimica et Biophysica Acta - Bioenergetics. 1837, 7, p. 954-963 10 p.

Research output: Contribution to journalArticle

Phenothiazines
Vitamin K 2
NAD
Staphylococcus aureus
Oxidoreductases
2015

Division of labor in transhydrogenase by alternating proton translocation and hydride transfer

Leung, J. H., Schurig-Briccio, L. A., Yamaguchi, M., Moeller, A., Speir, J. A., Gennis, R. B. & Stout, C. D., Jan 9 2015, In : Science. 347, 6218, p. 178-181 4 p.

Research output: Contribution to journalArticle

NADP
Protons
NADP Transhydrogenases
Thermus thermophilus
Proton-Motive Force
2013

Solid-state NMR study of a 41 kDa membrane protein complex DsbA/DsbB

Sperling, L. J., Tang, M., Berthold, D. A., Nesbitt, A. E., Gennis, R. B. & Rienstra, C. M., May 23 2013, In : Journal of Physical Chemistry B. 117, 20, p. 6052-6060 9 p.

Research output: Contribution to journalArticle

Chemical shift
chemical equilibrium
Catalytic Domain
Membrane Proteins
Nuclear magnetic resonance

Proton uptake and pKa changes in the uncoupled Asn139Cys variant of cytochrome c oxidase

Johansson, A. L., Carlsson, J., Högbom, M., Hosler, J. P., Gennis, R. B. & Brzezinski, P., Feb 5 2013, In : Biochemistry. 52, 5, p. 827-836 10 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Protons
Membrane Proteins
Membranes
Proton Pumps
2010

Functional interactions between membrane-bound transporters and membranes

Öjemyr, L. N., Lee, H. J., Gennis, R. B. & Brzezinski, P., Sep 7 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 36, p. 15763-15767 5 p.

Research output: Contribution to journalArticle

Membrane Transport Proteins
Membranes
Protons
Membrane Proteins
Respiratory Transport
2018

X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds

Schieferstein, J. M., Pawate, A. S., Varel, M. J., Guha, S., Astrauskaite, I., Gennis, R. B. & Kenis, P. J. A., Jan 1 2018, In : Lab on a chip. 18, 6, p. 944-954 11 p.

Research output: Contribution to journalArticle

Microfluidics
Crystallization
Membrane Proteins
X-Rays
Proteins
2015

Plasticity in the High Affinity Menaquinone Binding Site of the Cytochrome aa3-600 Menaquinol Oxidase from Bacillus subtilis

Yi, S. M., Taguchi, A. T., Samoilova, R. I., O'Malley, P. J., Gennis, R. B. & Dikanov, S. A., Jul 21 2015, In : Biochemistry. 54, 32, p. 5030-5044 15 p.

Research output: Contribution to journalArticle

Vitamin K 2
Electron Transport Complex IV
Bacilli
Bacillus subtilis
Plasticity
2011

High-resolution membrane protein structure by joint calculations with solid-state NMR and X-ray experimental data

Tang, M., Sperling, L. J., Berthold, D. A., Schwieters, C. D., Nesbitt, A. E., Nieuwkoop, A. J., Gennis, R. B. & Rienstra, C. M., Nov 1 2011, In : Journal of Biomolecular NMR. 51, 3, p. 227-233 7 p.

Research output: Contribution to journalArticle

Nuclear magnetic resonance spectroscopy
Membrane Proteins
Magnetic Resonance Spectroscopy
Joints
X-Rays
2016

A Purple Cupredoxin from Nitrosopumilus maritimus Containing a Mononuclear Type 1 Copper Center with an Open Binding Site

Hosseinzadeh, P., Tian, S., Marshall, N. M., Hemp, J., Mullen, T., Nilges, M. J., Gao, Y. G., Robinson, H., Stahl, D. A., Gennis, R. B. & Lu, Y., May 25 2016, In : Journal of the American Chemical Society. 138, 20, p. 6324-6327 4 p.

Research output: Contribution to journalArticle

Binding sites
Copper
Binding Sites
electron spin resonance
copper

The unusual redox properties of C-type oxidases

Melin, F., Xie, H., Meyer, T., Ahn, Y. O., Gennis, R. B., Michel, H. & Hellwig, P., Dec 1 2016, In : Biochimica et Biophysica Acta - Bioenergetics. 1857, 12, p. 1892-1899 8 p.

Research output: Contribution to journalArticle

Heme
Oxidation-Reduction
Oxidoreductases
Protons
Pseudomonas stutzeri
2019

Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli

Galván, A. E., Chalón, M. C., Ríos Colombo, N. S., Schurig-Briccio, L. A., Sosa-Padilla, B., Gennis, R. B. & Bellomio, A., May 2019, In : Biochimie. 160, p. 141-147 7 p.

Research output: Contribution to journalArticle

Cytochromes
Escherichia coli
Peptides
Cyanides
Electron Transport
2018

Cytochromes bd-I and bo3 are essential for the bactericidal effect of microcin J25 on Escherichia coli cells

Galván, A. E., Chalón, M. C., Schurig-Briccio, L. A., Salomón, R. A., Minahk, C. J., Gennis, R. B. & Bellomio, A., Feb 2018, In : Biochimica et Biophysica Acta - Bioenergetics. 1859, 2, p. 110-118 9 p.

Research output: Contribution to journalArticle

Open Access
Cytochromes
Escherichia coli
Cell Respiration
Oxygen
Membranes
2013

Single mutations that redirect internal proton transfer in the ba 3 oxidase from thermus thermophilus

Smirnova, I., Chang, H. Y., Von Ballmoos, C., Ädelroth, P., Gennis, R. B. & Brzezinski, P., Oct 8 2013, In : Biochemistry. 52, 40, p. 7022-7030 9 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Proton transfer
Protons
Oxidoreductases
Mutation
2014

Kinetics and intermediates of the reaction of fully reduced escherichia coli bo 3 ubiquinol oxidase with O2

Szundi, I., Kittredge, C., Choi, S. K., McDonald, W., Ray, J., Gennis, R. B. & Einarsdóttir, Ó., Jan 1 2014, In : Biochemistry. 53, 33, p. 5393-5404 12 p.

Research output: Contribution to journalArticle

Escherichia coli
Heme
Kinetics
Cytochromes
Oxidation

Proton pumping by an inactive structural variant of cytochrome c oxidase

Svahn, E., Faxén, K., Gennis, R. B. & Brzezinski, P., Nov 2014, In : Journal of Inorganic Biochemistry. 140, p. 6-11 6 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Protons
Catalytic Domain
Paracoccus denitrificans
Proton transfer
2019
Vitamin K 2
Cytochromes
Escherichia coli
Binding Sites
coenzyme Q10
2010

A pathogenic mutation in cytochrome c oxidase results in impaired proton pumping while retaining O2-reduction activity

Namslauer, I., Lee, H. J., Gennis, R. B. & Brzezinski, P., May 1 2010, In : Biochimica et Biophysica Acta - Bioenergetics. 1797, 5, p. 550-556 7 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Proton transfer
Protons
Mutation
Substitution reactions
2014

The KC channel in the cbb3-type respiratory oxygen reductase from Rhodobacter capsulatus is required for both chemical and pumped protons

Yildiz, G. G., Gennis, R. B., Daldal, F. & Öztürk, M., 2014, In : Journal of bacteriology. 196, 10, p. 1825-1832 8 p.

Research output: Contribution to journalArticle

Rhodobacter capsulatus
Protons
Oxidoreductases
Oxygen
Heme
2013

Alternate pathways for NADH oxidation in Thermus thermophilus using type 2 NADH dehydrogenases

Venkatakrishnan, P., Lencina, A. M., Schurig-Briccio, L. A. & Gennis, R. B., May 1 2013, In : Biological Chemistry. 394, 5, p. 667-676 10 p.

Research output: Contribution to journalArticle

Thermus thermophilus
NADH Dehydrogenase
NAD
Genes
Oxidation
2015

Review and Hypothesis. New insights into the reaction mechanism of transhydrogenase: Swivelling the dIII component may gate the proton channel

Jackson, J. B., Leung, J. H., Stout, C. D., Schurig-Briccio, L. A. & Gennis, R. B., Jul 20 2015, In : FEBS Letters. 589, 16, p. 2027-2033 7 p.

Research output: Contribution to journalReview article

NADP
Protons
Thermus thermophilus
Mitochondria
Enzymes
2018

Mechanism of proton transfer through the KC proton pathway in the Vibrio cholerae cbb3 terminal oxidase

Ahn, Y. O., Albertsson, I., Gennis, R. B. & Ädelroth, P., Nov 2018, In : Biochimica et Biophysica Acta - Bioenergetics. 1859, 11, p. 1191-1198 8 p.

Research output: Contribution to journalArticle

Proton transfer
Vibrio cholerae
Protons
Oxidoreductases
Oxygen
2010
Rhodobacter sphaeroides
Electron Transport Complex IV
Heme
Electrons
Protons
2019

Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus

Lencina, A. M., Koepke, J., Preu, J., Muenke, C., Gennis, R. B., Michel, H. & Schurig-Briccio, L. A., Nov 1 2019, In : Biochimica et Biophysica Acta - Bioenergetics. 1860, 11, 148080.

Research output: Contribution to journalArticle

Thermus thermophilus
Vitamin K 3
NAD
Oxidoreductases
X-Rays
2017

Dynamics of the KB Proton Pathway in Cytochrome ba3 from Thermus thermophilus

von Ballmoos, C., Smirnova, I., Poiana, F., Gonska, N., Chang, H. Y., Gennis, R. B., Brzezinski, P. & Ädelroth, P., May 1 2017, In : Israel Journal of Chemistry. 57, 5, p. 424-436 13 p.

Research output: Contribution to journalArticle

Protons
Oxidoreductases
Proton transfer
Pumps
Proton Pumps
2016

CtaM is required for menaquinol oxidase aa 3 function in Staphylococcus aureus

Hammer, N. D., Schurig-Briccio, L. A., Gerdes, S. Y., Gennis, R. B. & Skaar, E. P., Jul 1 2016, In : mBio. 7, 4, e00823-16.

Research output: Contribution to journalArticle

Staphylococcus aureus
Oxidoreductases
Electron Transport
Heme
Oxygen
2010

Conformational transitions and molecular hysteresis of cytochrome c oxidase: Varying the redox state by electronic wiring

Nowak, C., Santonicola, M. G., Schach, D., Zhu, J., Gennis, R. B., Ferguson-Miller, S., Baurecht, D., Walz, D., Knoll, W. & Naumann, R. L. C., Nov 7 2010, In : Soft Matter. 6, 21, p. 5523-5532 10 p.

Research output: Contribution to journalArticle

wiring
oxidase
cytochromes
Electron Transport Complex IV
Electric wiring
2014

Conformational coupling between the active site and residues within the KC-channel of the Vibrio cholerae cbb3-type (C-family) oxygen reductase

Ahn, Y. O., Mahinthichaichan, P., Lee, H. J., Ouyang, H., Kaluka, D., Yeh, S. R., Arjona, D., Rousseau, D. L., Tajkhorshid, E., Ädelroth, P. & Gennis, R. B., Oct 21 2014, In : Proceedings of the National Academy of Sciences of the United States of America. 111, 42, p. E4419-E4428

Research output: Contribution to journalArticle

Vibrio cholerae
Catalytic Domain
Oxidoreductases
Oxygen
Protons
2010

Intricate role of water in proton transport through cytochrome c oxidase

Lee, H. J., Svahn, E., Swanson, J. M. J., Lepp, H., Voth, G. A., Brzezinski, P. & Gennis, R. B., Nov 17 2010, In : Journal of the American Chemical Society. 132, 45, p. 16225-16239 15 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
cytochrome
Protons
mutation
Water
2011

Kinetic design of the respiratory oxidases

Von Ballmoos, C., Gennis, R. B., Ädelroth, P. & Brzezinski, P., Jul 5 2011, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 27, p. 11057-11062 6 p.

Research output: Contribution to journalArticle

Protons
Oxidoreductases
Electrons
Catalytic Domain
Thermus thermophilus
2013

Role of aspartate 132 at the orifice of a proton pathway in cytochrome c oxidase

Johansson, A. L., Högbom, M., Carlsson, J., Gennis, R. B. & Brzezinski, P., May 28 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 22, p. 8912-8917 6 p.

Research output: Contribution to journalArticle

Electron Transport Complex IV
Aspartic Acid
Protons
Biological Phenomena
Membranes

Structure of the disulfide bond generating membrane protein DsbB in the lipid bilayer

Tang, M., Nesbitt, A. E., Sperling, L. J., Berthold, D. A., Schwieters, C. D., Gennis, R. B. & Rienstra, C. M., May 27 2013, In : Journal of Molecular Biology. 425, 10, p. 1670-1682 13 p.

Research output: Contribution to journalArticle

Lipid Bilayers
Molecular Dynamics Simulation
Disulfides
Membrane Proteins
X-Rays