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2010

Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars

Nair, N. U. & Zhao, H., Sep 1 2010, In : Metabolic Engineering. 12, 5, p. 462-468 7 p.

Research output: Contribution to journalArticle

Xylitol
Arabinose
Xylose
Sugars
Aldehyde Reductase
2014

Tandem catalytic conversion of glucose to 5-hydroxymethylfurfural with an immobilized enzyme and a solid acid

Huang, H., Denard, C. A., Alamillo, R., Crisci, A. J., Miao, Y., Dumesic, J. A., Scott, S. L. & Zhao, H., Jul 3 2014, In : ACS Catalysis. 4, 7, p. 2165-2168 4 p.

Research output: Contribution to journalArticle

Immobilized Enzymes
Glucose
xylose isomerase
Enzymes
Fructose
2012

Mutagenic inverted repeats assisted genome engineering (MIRAGE) in saccharomyces cerevisiae: Deletion of gal7

Nair, N. U. & Zhao, H., Jan 20 2012, Microbial Metabolic Engineering: Methods and Protocols. Cheng, Q. (ed.). p. 63-73 11 p. (Methods in Molecular Biology; vol. 834).

Research output: Chapter in Book/Report/Conference proceedingChapter

Synthetic Biology
Metabolic Engineering
Systems Biology
Point Mutation
Saccharomyces cerevisiae
2011

Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD)

Tiwari, M. K., Lee, J. K., Moon, H. J. & Zhao, H., May 15 2011, In : Bioorganic and Medicinal Chemistry Letters. 21, 10, p. 2873-2876 4 p.

Research output: Contribution to journalArticle

Oxygenases
Mutagenesis
Dimerization
Operon
Site-Directed Mutagenesis
2010

Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis

Du, J., Li, S. & Zhao, H., Nov 1 2010, In : Molecular BioSystems. 6, 11, p. 2150-2156 7 p.

Research output: Contribution to journalArticle

Neurospora crassa
Pichia
Xylose
Pentoses
Saccharomyces cerevisiae
2013

Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

Li, S., Ha, S. J., Kim, H. J., Galazka, J. M., Cate, J. H. D., Jin, Y-S. & Zhao, H., Oct 10 2013, In : Journal of Biotechnology. 168, 1, p. 1-6 6 p.

Research output: Contribution to journalArticle

Cellobiose
Cell growth
Yeast
Fermentation
Glucose
2014

A single-chain TALEN architecture for genome engineering

Sun, N. & Zhao, H., Mar 1 2014, In : Molecular BioSystems. 10, 3, p. 446-453 8 p.

Research output: Contribution to journalArticle

Genome
DNA Cleavage
Peptides
Deoxyribonuclease I
Dimerization
2015

Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic

Badur, A. H., Jagtap, S. S., Yalamanchili, G., Lee, J. K., Zhao, H. & Rao, C. V., Jan 1 2015, In : Applied and environmental microbiology. 81, 5, p. 1865-1873 9 p.

Research output: Contribution to journalArticle

Vibrio splendidus
Vibrio
alginate
alginates
lyases
2012

Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease

Sun, N., Liang, J., Abil, Z. & Zhao, H., Mar 1 2012, In : Molecular BioSystems. 8, 4, p. 1255-1263 9 p.

Research output: Contribution to journalArticle

Sickle Cell Anemia
Therapeutics
Targeted Gene Repair
Globins
Transcription Activator-Like Effectors
2019

An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In : Nature chemical biology.

Research output: Contribution to journalArticle

Gene Knock-In Techniques
Ribonucleoproteins
Neoplastic Stem Cells
HEK293 Cells
DNA
2017

Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

Abil, Z., Gumy, L. F., Zhao, H. & Hoogenraad, C. C., Jun 16 2017, In : ACS synthetic biology. 6, 6, p. 950-956 7 p.

Research output: Contribution to journalArticle

RNA-Binding Proteins
RNA
Messenger RNA
Eukaryotic Cells
Gene expression
2015

Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae

Eriksen, D. T., HamediRad, M., Yuan, Y. & Zhao, H., Jul 17 2015, In : ACS synthetic biology. 4, 7, p. 808-814 7 p.

Research output: Contribution to journalArticle

Biosynthetic Pathways
Fatty acids
Yeast
Saccharomyces cerevisiae
Esters
2011

Combinatorial optimization of a highly efficient xylose utilization pathway in saccharomyces cerevisiae

Du, J., Kim, B. & Zhao, H., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xylose
Combinatorial optimization
Yeast
Pentoses
Enzymes
2014

Characterization and engineering of the adenylation domain of a NRPS-like protein: A potential biocatalyst for aldehyde generation

Wang, M. & Zhao, H., Apr 4 2014, In : ACS Catalysis. 4, 4, p. 1219-1225 7 p.

Research output: Contribution to journalArticle

Peptide Synthases
Biocatalysts
Staphylococcal Protein A
Aldehydes
Peptides
2013

Transcription activator-like effector nucleases (TALENs): a highly efficient and versatile tool for genome editing.

Sun, N. & Zhao, H., Jul 2013, In : Biotechnology and bioengineering. 110, 7, p. 1811-1821 11 p.

Research output: Contribution to journalReview article

Transcription
Genes
Technology
DNA
DNA sequences
2010

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae

Li, S., Du, J., Sun, J., Galazka, J. M., Glass, N. L., Cate, J. H. D., Yang, X. & Zhao, H., Nov 1 2010, In : Molecular BioSystems. 6, 11, p. 2129-2132 4 p.

Research output: Contribution to journalArticle

Glucosidases
Cellobiose
Xylose
Fermentation
Saccharomyces cerevisiae
saccharification
Cellulose
Fermentation
fermentation
yeast
2013

Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production

Rao, G., Lee, J. K. & Zhao, H., Jul 1 2013, In : Applied Microbiology and Biotechnology. 97, 13, p. 5861-5867 7 p.

Research output: Contribution to journalArticle

Phloroglucinol
Malonyl Coenzyme A
Escherichia coli
Protein Engineering
Poisons
2015

Improving and repurposing biocatalysts via directed evolution

Denard, C. A., Ren, H. & Zhao, H., Apr 1 2015, In : Current Opinion in Chemical Biology. 25, p. 55-64 10 p.

Research output: Contribution to journalReview article

Enzymes
Biocatalysis
Protein Engineering
Stereoselectivity
Chemical reactivity
2012

CmlI is an N-oxygenase in the biosynthesis of chloramphenicol

Lu, H., Chanco, E. & Zhao, H., Sep 16 2012, In : Tetrahedron. 68, 37, p. 7651-7654 4 p.

Research output: Contribution to journalArticle

Oxygenases
Biosynthesis
Chloramphenicol
Enzymes
Ferrozine
2017
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
2019

Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides

Schultz, J. C., Cao, M. & Zhao, H., Aug 2019, In : Biotechnology and bioengineering. 116, 8, p. 2103-2109 7 p.

Research output: Contribution to journalArticle

Clustered Regularly Interspaced Short Palindromic Repeats
Gene Knockout Techniques
Gene Deletion
Guide RNA
Genes
2016
Antibiotics
Yeast
Saccharomyces cerevisiae
Plasmids
Genes
2015

Development of a One-Pot tandem reaction combining ruthenium-catalyzed alkene metathesis and enantioselective enzymatic oxidation to produce Aryl epoxides

Denard, C. A., Bartlett, M. J., Wang, Y., Lu, L., Hartwig, J. F. & Zhao, H., Jun 5 2015, In : ACS Catalysis. 5, 6, p. 3817-3822 6 p.

Research output: Contribution to journalArticle

Ruthenium
Epoxy Compounds
Alkenes
Olefins
Oxidation
2014

DNA assembly techniques for next-generation combinatorial biosynthesis of natural products

Cobb, R. E., Ning, J. C. & Zhao, H., Feb 1 2014, In : Journal of Industrial Microbiology and Biotechnology. 41, 2, p. 469-477 9 p.

Research output: Contribution to journalReview article

Biosynthesis
Biological Products
DNA
Synthetic Biology
Cloning
2018

Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes

Tapsin, S., Sun, M., Shen, Y., Zhang, H., Lim, X. N., Susanto, T. T., Yang, S. L., Zeng, G. S., Lee, J., Lezhava, A., Ang, E. L., Zhang, L. H., Wang, Y., Zhao, H., Nagarajan, N. & Wan, Y., Dec 1 2018, In : Nature communications. 9, 1, 1289.

Research output: Contribution to journalArticle

prokaryotes
Nucleotide Aptamers
eukaryotes
Flavin Mononucleotide
genome
2013

Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands

Liang, J., Mclachlan, M. J. & Zhao, H., May 1 2013, In : Biotechnology and bioengineering. 110, 5, p. 1419-1429 11 p.

Research output: Contribution to journalArticle

Switch Genes
Artificial Cells
Gene expression
Genes
Ligands
2011

Drug Discovery and Development by Combinatorial Biosynthesis

Desieno, M. A., Denard, C. A. & Zhao, H., May 25 2011, Enzyme Technologies: Metagenomics, Evolution, Biocatalysis, and Biosynthesis. John Wiley and Sons, p. 251-283 33 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Biosynthesis
Drug Discovery

Engineering saccharomyces cerevisiae for biofuel production from lignocellulosic biomass

Du, J. & Zhao, H., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pentoses
Biofuels
Yeast
Biomass
Xylose
2012

DNA assembler: A synthetic biology tool for characterizing and engineering natural product gene clusters

Shao, Z. & Zhao, H., 2012, Natural Product Biosynthesis by Microorganisms and Plants, Part C. Academic Press Inc., p. 203-224 22 p. (Methods in Enzymology; vol. 517).

Research output: Chapter in Book/Report/Conference proceedingChapter

Synthetic Biology
Biosynthetic Pathways
Multigene Family
Biological Products
Genes
2015

Recent advances in combinatorial biosynthesis for drug discovery

Sun, H., Liu, Z., Zhao, H. & Ang, E. L., Feb 12 2015, In : Drug Design, Development and Therapy. 9, p. 823-833 11 p.

Research output: Contribution to journalReview article

Drug Discovery
Biological Products
Biosynthetic Pathways
Pharmaceutical Preparations
Poisons
2012

A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency

Jeya, M., Kim, T. S., Kumar Tiwari, M., Li, J., Zhao, H. & Lee, J. K., Dec 2012, In : Molecular BioSystems. 8, 12, p. 3103-3106 4 p.

Research output: Contribution to journalArticle

Rhizobium etli
Pyrones
Malonyl Coenzyme A
Acetyl Coenzyme A
Molecular Dynamics Simulation

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

Wen, S., Tan, T. & Zhao, H., Dec 5 2012, In : Journal of Biotechnology. 164, 2, p. 248-253 6 p.

Research output: Contribution to journalArticle

Yarrowia
Lipases
Lipase
Enzymes
Half-Life
2013

Directed evolution: Past, present, and future

Cobb, R. E., Chao, R. & Zhao, H., May 1 2013, In : AIChE Journal. 59, 5, p. 1432-1440 9 p.

Research output: Contribution to journalArticle

Screening
Proteins
Biological Phenomena
Mutagenesis
Polymerase chain reaction

Construction and engineering of large biochemical pathways via DNA assembler

Shao, Z. & Zhao, H., Sep 20 2013, Synthetic Biology. Polizzi, K. & Kontoravdi, C. (eds.). p. 85-106 22 p. (Methods in Molecular Biology; vol. 1073).

Research output: Chapter in Book/Report/Conference proceedingChapter

Multigene Family
DNA
Synthetic Biology
Metabolic Engineering
Homologous Recombination
2011

Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo

Evans, B. S., Chen, Y., Metcalf, W. W., Zhao, H. & Kelleher, N. L., May 27 2011, In : Chemistry and Biology. 18, 5, p. 601-607 7 p.

Research output: Contribution to journalArticle

Peptide Synthases
Reengineering
Derivatives
Enzymes
Pantoea
2013

Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial saccharomyces cerevisiae strain

Yuan, Y. & Zhao, H., Nov 1 2013, In : Biotechnology and bioengineering. 110, 11, p. 2874-2881 8 p.

Research output: Contribution to journalArticle

Cellobiose
Yeast
Saccharomyces cerevisiae
Genes
Productivity
2014
Butylene Glycols
Metabolic engineering
Metabolic Engineering
Galactose
Yeast
2012

Recent advances in targeted genome engineering in mammalian systems

Sun, N., Abil, Z. & Zhao, H., Sep 1 2012, In : Biotechnology Journal. 7, 9, p. 1074-1087 14 p.

Research output: Contribution to journalReview article

Genome
Homologous Recombination
Gene Targeting
Double-Stranded DNA Breaks
Endonucleases
2013

Modular assembly of designer Puf proteins for specific post-transcriptional regulation of endogenous RNA

Abil, Z. & Zhao, H., Jan 1 2013, Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

RNA
Proteins
proteins
2012

Strain Improvement via Evolutionary Engineering

Kim, B., Du, J. & Zhao, H., Oct 24 2012, Engineering Complex Phenotypes in Industrial Strains. John Wiley and Sons, p. 111-131 21 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2015

Reversal of the β-oxidation cycle in saccharomyces cerevisiae for production of fuels and chemicals

Lian, J. & Zhao, H., Mar 20 2015, In : ACS synthetic biology. 4, 3, p. 332-341 10 p.

Research output: Contribution to journalArticle

Yeast
Saccharomyces cerevisiae
Fatty Acids
Oxidation
1-Butanol
2013

Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13C metabolic flux analysis

Feng, X. & Zhao, H., Sep 1 2013, In : AIChE Journal. 59, 9, p. 3195-3202 8 p.

Research output: Contribution to journalArticle

Metabolic Flux Analysis
Xylose
Metabolism
Yeast
Glucose
2012

DNA assembler method for construction of zeaxanthin-producing strains of saccharomyces cerevisiae

Shao, Z., Luo, Y. & Zhao, H., Jul 27 2012, Microbial Carotenoids From Fungi: Methods and Protocols. Jose-Luis, B. (ed.). p. 251-262 12 p. (Methods in Molecular Biology; vol. 898).

Research output: Chapter in Book/Report/Conference proceedingChapter

Saccharomyces cerevisiae
Synthetic Biology
Metabolic Engineering
Homologous Recombination
DNA
2014

Preparing your manuscript for submission to ACS catalysis

Jones, C. W., Smith, D. J., Gunnoe, T. B., Zhao, H., Sautet, P., Scott, S. L. & Xu, B. Q., Jan 1 2014, In : ACS Catalysis. 4, 8, p. 2827-2828 2 p.

Research output: Contribution to journalReview article

Catalysis
2010

Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis

Choi, Y. S., Johannes, T. W., Simurdiak, M., Shao, Z., Lu, H. & Zhao, H., Feb 8 2010, In : Molecular BioSystems. 6, 2, p. 336-338 3 p.

Research output: Contribution to journalArticle

Streptomyces
Multigene Family
Organism Cloning
Polyketides
spectinabilin
2013

Customized optimization of metabolic pathways by combinatorial transcriptional engineering.

Yuan, Y., Du, J. & Zhao, H., Aug 2 2013, In : Methods in molecular biology (Clifton, N.J.). 985, p. 177-209 33 p.

Research output: Contribution to journalArticle

Metabolic Networks and Pathways
Synthetic Biology
Metabolic Engineering
Cellobiose
Xylose
2016

ACS Catalysis and the Scope of Papers Sought in Three Catalysis Subdisciplines: Biocatalysis and Enzymology, Molecular Catalysis for Organic Synthesis, and Heterogeneous Photocatalysis

Chang, S., Fornasiero, P., Gunnoe, T. B., Jones, C. W., Linic, S., Ooi, T., Williams, R. M. & Zhao, H., Jul 1 2016, In : ACS Catalysis. 6, 7, p. 4782-4785 4 p.

Research output: Contribution to journalReview article

Photocatalysis
Catalysis
Biocatalysis

Combined and iterative use of computational design and directed evolution for protein-ligand binding design

Wang, M. & Zhao, H., May 1 2016, Methods in Molecular Biology. Humana Press Inc., p. 139-153 15 p. (Methods in Molecular Biology; vol. 1414).

Research output: Chapter in Book/Report/Conference proceedingChapter

Protein Binding
Switch Genes
Ligands
Two-Hybrid System Techniques
Mutagenesis
Lipoylation
Pyruvic Acid
Cytosol
Yeast
Machinery