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2019
A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data
Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In: Metabolic Engineering Communications. 9, e00101.Research output: Contribution to journal › Article › peer-review
Open Access -
A continuing career in biocatalysis: Frances H. Arnold
Fasan, R., Jennifer Kan, S. B. & Zhao, H., Nov 1 2019, In: ACS Catalysis. 9, 11, p. 9775-9788 14 p.Research output: Contribution to journal › Article › peer-review
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Activation of silent biosynthetic gene clusters using transcription factor decoys
Wang, B., Guo, F., Dong, S. H. & Zhao, H., Feb 1 2019, In: Nature chemical biology. 15, 2, p. 111-114 4 p.Research output: Contribution to journal › Article › peer-review
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A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium
Xing, M., Wei, Y., Hua, G., Li, M., Nanjaraj Urs, A. N., Wang, F., Hu, Y., Zhai, W., Liu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Aug 15 2019, In: Biochemical Journal. 476, 15, p. 2271-2279 9 p.Research output: Contribution to journal › Article › peer-review
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An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms
Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In: Nature chemical biology. 16, 4, p. 387-390 4 p.Research output: Contribution to journal › Article › peer-review
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An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine
Yin, J., Wei, Y., Liu, D., Hu, Y., Lu, Q., Lui Ang, E., Zhao, H. & Zhang, Y., Oct 25 2019, In: Journal of Biological Chemistry. 294, 43, p. 15662-15671 10 p.Research output: Contribution to journal › Article › peer-review
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A pathway for isethionate dissimilation in Bacillus krulwichiae
Tong, Y., Wei, Y., Hu, Y., Ang, E. L., Zhao, H. & Zhang, Y., 2019, In: Applied and environmental microbiology. 85, 15, e00793-19.Research output: Contribution to journal › Article › peer-review
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Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca
Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Biochemical Journal. 476, 4, p. 733-746 14 p.Research output: Contribution to journal › Article › peer-review
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Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense
Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In: Biochemical Journal. 476, 11, p. 1605-1619 15 p.Research output: Contribution to journal › Article › peer-review
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Building a global alliance of biofoundries
Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, , Dec 1 2019, In: Nature communications. 10, 1, 2040.Research output: Contribution to journal › Comment/debate › peer-review
Open Access -
Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes
Yeo, W. L., Heng, E., Tan, L. L., Lim, Y. W., Lim, Y. H., Hoon, S., Zhao, H., Zhang, M. M. & Wong, F. T., Sep 2019, In: Biotechnology and bioengineering. 116, 9, p. 2330-2338 9 p.Research output: Contribution to journal › Article › peer-review
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Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098
Nguyen, K., DeSieno, M. A., Bae, B., Johannes, T. W., Cobb, R. E., Zhao, H. & Nair, S. K., 2019, In: Organic and Biomolecular Chemistry. 17, 6, p. 1506-1518 13 p.Research output: Contribution to journal › Article › peer-review
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Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification
Wen, F., Smith, M. R. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 213-234 22 p. (Methods in Molecular Biology; vol. 2024).Research output: Chapter in Book/Report/Conference proceeding › Chapter
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Delta integration CRISPR-Cas (Di-CRISPR) in saccharomyces cerevisiae
Shi, S., Liang, Y., Ang, E. L. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 73-91 19 p. (Methods in Molecular Biology; vol. 1927).Research output: Chapter in Book/Report/Conference proceeding › Chapter
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Development of a CRISPR/Cas9-based tool for gene deletion in Issatchenkia orientalis
Tran, V. G., Cao, M., Fatma, Z., Song, X. & Zhao, H., May 1 2019, In: mSphere. 4, 3, e00345-19.Research output: Contribution to journal › Article › peer-review
Open Access -
Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides
Schultz, J. C., Cao, M. & Zhao, H., Aug 2019, In: Biotechnology and bioengineering. 116, 8, p. 2103-2109 7 p.Research output: Contribution to journal › Article › peer-review
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Highly Efficient Single-Pot Scarless Golden Gate Assembly
Hamedirad, M., Weisberg, S., Chao, R., Lian, J. & Zhao, H., May 17 2019, In: ACS synthetic biology. 8, 5, p. 1047-1054 8 p.Research output: Contribution to journal › Article › peer-review
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Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense
Zhou, Y., Wei, Y., Nanjaraj Urs, A. N., Lin, L., Xu, T., Hu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Bioscience Reports. 39, 6, Y.Research output: Contribution to journal › Article › peer-review
Open Access -
Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping
Lian, J., Schultz, C., Cao, M., HamediRad, M. & Zhao, H., Dec 1 2019, In: Nature communications. 10, 1, 5794.Research output: Contribution to journal › Article › peer-review
Open Access -
Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria
Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., N. Nanjaraj Urs, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Dec 1 2019, In: Nature communications. 10, 1, 1609.Research output: Contribution to journal › Article › peer-review
Open Access -
Towards a fully automated algorithm driven platform for biosystems design
HamediRad, M., Chao, R., Weisberg, S., Lian, J., Sinha, S. & Zhao, H., Nov 13 2019, In: Nature communications. 10, 1, 5150.Research output: Contribution to journal › Article › peer-review
Open Access -
2018
A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance
Sun, H., Zhao, H. & Ang, E. L., 2018, In: Chemical Communications. 54, 68, p. 9458-9461 4 p.Research output: Contribution to journal › Article › peer-review
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Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In: Biotechnology Journal. 13, 9, 1700601.Research output: Contribution to journal › Review article › peer-review
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Applying advanced dna assembly methods to generate pathway libraries
Eriksen, D. T., Chao, R. & Zhao, H., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 331-347 17 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter
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Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation
Lim, Y. H., Wong, F. T., Yeo, W. L., Ching, K. C., Lim, Y. W., Heng, E., Chen, S., Tsai, D. J., Lauderdale, T. L., Shia, K. S., Ho, Y. S., Hoon, S., Ang, E. L., Zhang, M. M. & Zhao, H., Aug 16 2018, In: ChemBioChem. 19, 16, p. 1716-1719 4 p.Research output: Contribution to journal › Article › peer-review
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Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates
Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In: Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.Research output: Contribution to journal › Review article › peer-review
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Cooperative asymmetric reactions combining photocatalysis and enzymatic catalysis
Litman, Z. C., Wang, Y., Zhao, H. & Hartwig, J. F., Aug 16 2018, In: Nature. 560, 7718, p. 355-359 5 p.Research output: Contribution to journal › Article › peer-review
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CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci
Tasan, I., Sustackova, G., Zhang, L., Kim, J., Sivaguru, M., HamediRad, M., Wang, Y., Genova, J., Ma, J., Belmont, A. S. & Zhao, H., Sep 28 2018, In: Nucleic acids research. 46, 17, e100.Research output: Contribution to journal › Article › peer-review
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Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface
Limsakul, P., Peng, Q., Wu, Y., Allen, M. E., Liang, J., Remacle, A. G., Lopez, T., Ge, X., Kay, B. K., Zhao, H., Strongin, A. Y., Yang, X. L., Lu, S. & Wang, Y., Apr 19 2018, In: Cell chemical biology. 25, 4, p. 370-379.e4Research output: Contribution to journal › Article › peer-review
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Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains
Lian, J., Bao, Z., Hu, S. & Zhao, H., Jun 2018, In: Biotechnology and bioengineering. 115, 6, p. 1630-1635 6 p.Research output: Contribution to journal › Article › peer-review
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Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production
Wang, Y., Ren, H. & Zhao, H., Mar 4 2018, In: Critical Reviews in Biochemistry and Molecular Biology. 53, 2, p. 115-129 15 p.Research output: Contribution to journal › Review article › peer-review
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Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision
Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J. & Zhao, H., Jul 1 2018, In: Nature Biotechnology. 36, 6, p. 505-508 4 p.Research output: Contribution to journal › Article › peer-review
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Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes
Tapsin, S., Sun, M., Shen, Y., Zhang, H., Lim, X. N., Susanto, T. T., Yang, S. L., Zeng, G. S., Lee, J., Lezhava, A., Ang, E. L., Zhang, L. H., Wang, Y., Zhao, H., Nagarajan, N. & Wan, Y., Dec 1 2018, In: Nature communications. 9, 1, 1289.Research output: Contribution to journal › Article › peer-review
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Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole
Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A. N., Liu, Y., Ang, E. L., Zhao, S., Zhao, H. & Zhang, Y., Dec 1 2018, In: Nature communications. 9, 1, 4224.Research output: Contribution to journal › Article › peer-review
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Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction
Singh, R. K., Singh, R., Sivakumar, D., Kondaveeti, S., Kim, T., Li, J., Sung, B. H., Cho, B. K., Kim, D. R., Kim, S. C., Kalia, V. C., Zhang, Y. H. P. J., Zhao, H., Kang, Y. C. & Lee, J. K., Dec 7 2018, In: ACS Catalysis. 8, 12, p. 11085-11093 9 p.Research output: Contribution to journal › Article › peer-review
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In vivo biosensors: mechanisms, development, and applications
Shi, S., Ang, E. L. & Zhao, H., Jul 1 2018, In: Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 491-516 26 p.Research output: Contribution to journal › Article › peer-review
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Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion
Schultz, C., Lian, J. & Zhao, H., Jan 1 2018, Methods in Enzymology. Scrutton, N. (ed.). Academic Press Inc., p. 265-276 12 p. (Methods in Enzymology; vol. 608).Research output: Chapter in Book/Report/Conference proceeding › Chapter
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Pathway design, engineering, and optimization
Garcia-Ruiz, E., HamediRad, M. & Zhao, H., Jan 1 2018, Advances in Biochemical Engineering/Biotechnology. Springer Science and Business Media Deutschland GmbH, (Advances in Biochemical Engineering/Biotechnology; vol. 162).Research output: Chapter in Book/Report/Conference proceeding › Chapter
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Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system
Fine, J. D., Shpigler, H. Y., Ray, A. M., Beach, N. J., Sankey, A. L., Cash-Ahmed, A., Huang, Z. Y., Astrauskaite, I., Chao, R., Zhao, H. & Robinson, G. E., Sep 2018, In: PloS one. 13, 9, e0203444.Research output: Contribution to journal › Article › peer-review
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Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli
Ren, H., Biswas, S., Ho, S., Van Der Donk, W. A. & Zhao, H., Oct 19 2018, In: ACS chemical biology. 13, 10, p. 2966-2972 7 p.Research output: Contribution to journal › Article › peer-review
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Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli
Si, T., Tian, Q., Min, Y., Zhang, L., Sweedler, J. V., Van Der Donk, W. A. & Zhao, H., Sep 26 2018, In: Journal of the American Chemical Society. 140, 38, p. 11884-11888 5 p.Research output: Contribution to journal › Article › peer-review
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Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications
Lian, J., Mishra, S. & Zhao, H., Nov 2018, In: Metabolic Engineering. 50, p. 85-108 24 p.Research output: Contribution to journal › Review article › peer-review
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Recent developments in the application of P450 based biocatalysts
Wei, Y., Ang, E. L. & Zhao, H., Apr 2018, In: Current Opinion in Chemical Biology. 43, p. 1-7 7 p.Research output: Contribution to journal › Review article › peer-review
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RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization
HamediRad, M., Lian, J., Li, H. & Zhao, H., Jun 2018, In: Biotechnology and bioengineering. 115, 6, p. 1552-1560 9 p.Research output: Contribution to journal › Article › peer-review
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Synthetic biology advances and applications in the biotechnology industry: a perspective
Katz, L., Chen, Y. Y., Gonzalez, R., Peterson, T. C., Zhao, H. & Baltz, R. H., Jul 1 2018, In: Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 449-461 13 p.Research output: Contribution to journal › Article › peer-review
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Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding
Wang, P., Liang, J., Shi, L. Z., Wang, Y., Zhang, P., Ouyang, M., Preece, D., Peng, Q., Shao, L., Fan, J., Sun, J., Li, S. S., Berns, M. W., Zhao, H. & Wang, Y., Sep 19 2018, In: ACS Photonics. 5, 9, p. 3565-3574 10 p.Research output: Contribution to journal › Article › peer-review
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2017
A New Era of Genome Integration - Simply Cut and Paste!
Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 601-609 9 p.Research output: Contribution to journal › Review article › peer-review
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A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae
Ren, H., Hu, P. & Zhao, H., Aug 2017, In: Biotechnology and bioengineering. 114, 8, p. 1847-1854 8 p.Research output: Contribution to journal › Article › peer-review
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A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis
Xiong, X., Zhang, Y., Yan, J., Jain, S., Chee, S., Ren, B. & Zhao, H., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 1034-1042 9 p.Research output: Contribution to journal › Article › peer-review
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Automated multiplex genome-scale engineering in yeast
Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., 2017, In: Nature communications. 8, 15187.Research output: Contribution to journal › Article › peer-review