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• 2016

  Characterization of bacillus subtilis colony biofilms via mass spectrometry and fluorescence imaging

  Si, T., Li, B., Zhang, K., Xu, Y., Zhao, H. & Sweedler, J. V., Jun 3 2016, In: Journal of Proteome Research. 15, 6, p. 1955-1962 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Fluorescence imaging
  • Bacilli
  • Biofilms
  • Mass spectrometry
  • Optical Imaging

• Combinatorial pathway engineering for optimized production of the anti-malarial FR900098

  Freestone, T. S. & Zhao, H., Feb 1 2016, In: Biotechnology and bioengineering. 113, 2, p. 384-392 9 p.

  Research output: Contribution to journal › Article › peer-review

  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid
  • Antimalarials
  • Libraries
  • Screening
  • Organophosphonates

• Combined and iterative use of computational design and directed evolution for protein-ligand binding design

  Wang, M. & Zhao, H., May 1 2016, Methods in Molecular Biology. Humana Press Inc., p. 139-153 15 p. (Methods in Molecular Biology; vol. 1414).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Protein Binding
  • Switch Genes
  • Ligands
  • Two-Hybrid System Techniques
  • Mutagenesis

• Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

  Lian, J., Jin, R. & Zhao, H., Nov 1 2016, In: Biotechnology and bioengineering. 113, 11, p. 2462-2473 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Antibiotics
  • Yeast
  • Saccharomyces cerevisiae
  • Genes
  • Butenes

• CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

  Wendt, K. E., Ungerer, J., Cobb, R. E., Zhao, H. & Pakrasi, H. B., Jun 23 2016, In: Microbial cell factories. 15, 1, 115.

  Research output: Contribution to journal › Article › peer-review

  • Cyanobacteria
  • Mutagenesis
  • Synechococcus
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • CRISPR-Associated Protein 9

• Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae

  Wang, M., Li, S. & Zhao, H., Jan 1 2016, In: Biotechnology and bioengineering. 113, 1, p. 206-215 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Metabolites
  • Yeast
  • Gene Regulatory Networks
  • Gene expression

• Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate

  Sun, H., Yeo, W. L., Lim, Y. H., Chew, X., Smith, D. J., Xue, B., Chan, K. P., Robinson, R. C., Robins, E. G., Zhao, H. & Ang, E. L., Nov 7 2016, In: Angewandte Chemie - International Edition. 55, 46, p. 14277-14280 4 p.

  Research output: Contribution to journal › Article › peer-review

  • 5'-fluoro-5'-deoxyadenosine
  • fluorinase
  • 5'-chloro-5'-deoxyadenosine
  • Fluorination
  • Methionine

• Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization

  Wang, M., Yu, C. & Zhao, H., Mar 1 2016, In: Biotechnology and bioengineering. 113, 3, p. 484-491 8 p.

  Research output: Contribution to journal › Article › peer-review

  • xylose-glucose
  • Xylose
  • Glucose
  • Neurospora crassa
  • Biofuels

• Editorial: Synthetic biology and bioinformatics

  Medema, M. H. & Zhao, H., Aug 2016, In: Natural Product Reports. 33, 8, p. 913-914 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Engineered minicellulosomes for consolidated bioprocessing

  Liang, Y., Ang, E. L. & Zhao, H., Jul 11 2016, Handbook of Carbohydrate-Modifying Biocatalysts. Pan Stanford Publishing Pte. Ltd., Vol. 2. p. 965-992 28 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Engineering microbial hosts for production of bacterial natural products

  Zhang, M. M., Wang, Y., Ang, E. L. & Zhao, H., Aug 2016, In: Natural Product Reports. 33, 8, p. 963-987 25 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biological Products
  • Actinobacteria
  • Genetic Engineering
  • Genetic engineering
  • Fermentation

• Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

  Yuan, Y., Andersen, E. & Zhao, H., Jan 15 2016, In: ACS synthetic biology. 5, 1, p. 46-52 7 p.

  Research output: Contribution to journal › Article › peer-review

  • DNA
  • Plasmids
  • Yeast
  • Ligases
  • Networks (circuits)

• Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering

  Lian, J. & Zhao, H., Jul 15 2016, In: ACS synthetic biology. 5, 7, p. 689-697 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Oxidoreductases
  • Lipoylation
  • Yeast
  • Pyruvic Acid
  • Cytosol

• High-Efficiency Genome Editing of Streptomyces Species by an Engineered CRISPR/Cas System

  Wang, Y., Cobb, R. E. & Zhao, H., 2016, Methods in Enzymology. O'Connor, S. E. (ed.). Academic Press Inc., p. 271-284 14 p. (Methods in Enzymology; vol. 575).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • CRISPR-Cas Systems
  • Gene Editing
  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Genes

• High-throughput screening or selection methods for evolutionary enzyme engineering

  Shi, S., Zhang, H., Ang, E. L. & Zhao, H., May 17 2016, Understanding Enzymes: Function, Design, Engineering and Analysis. Pan Stanford Publishing Pte. Ltd., p. 707-744 38 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Identification and engineering of n-butanol biosensors by transcriptome analysis in yeast

  Shi, S., Ang, E. L., Tan, M. H. & Zhao, H., Jan 1 2016, Metabolic Engineering 11. AIChE, p. 458-459 2 p. (Metabolic Engineering 11).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Identification of an important motif that controls the activity and specificity of sugar transporters

  Wang, M., Yu, C. & Zhao, H., Jul 1 2016, In: Biotechnology and bioengineering. 113, 7, p. 1460-1467 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Sugars
  • Glucose
  • xylose-glucose
  • Facilitative Glucose Transport Proteins

• Members of the rusc protein family interact with Sufu and inhibit vertebrate hedgehog signaling

  Jin, Z., Schwend, T., Fu, J., Bao, Z., Liang, J., Zhao, H., Mei, W. & Yang, J., Nov 1 2016, In: Development (Cambridge). 143, 21, p. 3944-3955 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Hedgehogs
  • vertebrate
  • Vertebrates
  • family
  • protein

• Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae

  Shi, S., Si, T., Liu, Z., Zhang, H., Ang, E. L. & Zhao, H., May 10 2016, In: Scientific reports. 6, 25675.

  Research output: Contribution to journal › Article › peer-review

  • 1-Butanol
  • Metabolic Engineering
  • Saccharomyces cerevisiae
  • citramalate
  • Threonine

• Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose

  Guo, W., Sheng, J., Zhao, H. & Feng, X., Feb 1 2016, In: Microbial cell factories. 15, 1, 24.

  Research output: Contribution to journal › Article › peer-review

  • cetyl alcohol
  • Metabolic engineering
  • Xylose
  • Metabolic Engineering
  • Fatty Alcohols

• New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

  Luo, Y., Enghiad, B. & Zhao, H., Feb 2016, In: Natural Product Reports. 33, 2, p. 174-182 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Multigene Family
  • Biological Products
  • Genes
  • Metabolic engineering
  • Synthetic biology

• Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis

  Zhang, H., Li, X., Su, X., Ang, E. L., Zhang, Y. & Zhao, H., Apr 20 2016, In: ChemCatChem. 8, 8, p. 1500-1506 7 p.

  Research output: Contribution to journal › Article › peer-review

  • sugar beets
  • Sugar beets
  • adipic acid
  • Catalysis
  • catalysis

• Rapid and efficient galactose fermentation by engineered Saccharomyces cerevisiae

  Quarterman, J., Skerker, J. M., Feng, X., Liu, I. Y., Zhao, H., Arkin, A. P. & Jin, Y. S., Jul 10 2016, In: Journal of Biotechnology. 229, p. 13-21 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Yeast
  • Fermentation
  • Galactose
  • Saccharomyces cerevisiae
  • Ethanol

• RNAi-assisted genome evolution (RAGE) in Saccharomyces cerevisiae

  Si, T. & Zhao, H., Jan 1 2016, In: Methods in Molecular Biology. 1470, p. 183-198 16 p.

  Research output: Contribution to journal › Article › peer-review

  • RNA Interference
  • Saccharomyces cerevisiae
  • Genome
  • Acetic Acid
  • Libraries

• Standardization for natural product synthetic biology

  Zhao, H. & Medema, M. H., Aug 2016, In: Natural Product Reports. 33, 8, p. 920-924 5 p.

  Research output: Contribution to journal › Review article › peer-review

  • Synthetic biology
  • Synthetic Biology
  • Biological Products
  • Standardization
  • Multigene Family

• TALE proteins search DNA using a rotationally decoupled mechanism

  Cuculis, L., Abil, Z., Zhao, H. & Schroeder, C. M., Oct 1 2016, In: Nature chemical biology. 12, 10, p. 831-837 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effectors
  • DNA-Binding Proteins
  • DNA
  • Proteins
  • Nucleic Acid Conformation

• Tandem reactions combining biocatalysts and chemical catalysts for asymmetric synthesis

  Wang, Y. & Zhao, H., Dec 1 2016, In: Catalysts. 6, 12, 194.

  Research output: Contribution to journal › Review article › peer-review

  • Biocatalysts
  • catalysts
  • Catalysis
  • Catalysts
  • catalysis

• Use of genome-editing tools to treat sickle cell disease

  Tasan, I., Jain, S. & Zhao, H., Sep 1 2016, In: Human Genetics. 135, 9, p. 1011-1028 18 p.

  Research output: Contribution to journal › Review article › peer-review

  • Gene Editing
  • Sickle Cell Anemia
  • Globins
  • CRISPR-Associated Protein 9
  • Zinc Finger Nucleases
• 2017

  A New Era of Genome Integration - Simply Cut and Paste!

  Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 601-609 9 p.

  Research output: Contribution to journal › Review article › peer-review

  • Ointments
  • Genes
  • Homologous Recombination
  • Genome
  • Synthetic Biology

• A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae

  Ren, H., Hu, P. & Zhao, H., Aug 2017, In: Biotechnology and bioengineering. 114, 8, p. 1847-1854 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Escherichia coli
  • Yeast
  • Workflow
  • Biological Products
  • Saccharomyces cerevisiae

• A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis

  Xiong, X., Zhang, Y., Yan, J., Jain, S., Chee, S., Ren, B. & Zhao, H., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 1034-1042 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Epitopes
  • Chromatin Immunoprecipitation
  • Genes
  • Chromatin
  • Throughput

• Automated multiplex genome-scale engineering in yeast

  Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., 2017, In: Nature communications. 8, 15187.

  Research output: Contribution to journal › Article › peer-review

  • genome
  • yeast
  • Yeast
  • Genes
  • Yeasts

• Breaking the silence: new strategies for discovering novel natural products

  Ren, H., Wang, B. & Zhao, H., Dec 1 2017, In: Current Opinion in Biotechnology. 48, p. 21-27 7 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biological Products
  • Genes
  • Multigene Family
  • Throughput
  • Pharmaceutical Preparations

• Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

  Lian, J., Hamedirad, M., Hu, S. & Zhao, H., Dec 1 2017, In: Nature communications. 8, 1, 1688.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic engineering
  • Metabolic Engineering
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • yeast
  • Yeast

• Combining Rh-Catalyzed Diazocoupling and Enzymatic Reduction to Efficiently Synthesize Enantioenriched 2-Substituted Succinate Derivatives

  Wang, Y., Bartlett, M. J., Denard, C. A., Hartwig, J. F. & Zhao, H., Apr 7 2017, In: ACS Catalysis. 7, 4, p. 2548-2552 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Succinic Acid
  • Alkenes
  • Oxidoreductases
  • Derivatives
  • Olefins

• CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

  Zhang, M. M., Wong, F. T., Wang, Y., Luo, S., Lim, Y. H., Heng, E., Yeo, W. L., Cobb, R. E., Enghiad, B., Ang, E. L. & Zhao, H., Jun 1 2017, In: Nature chemical biology. 13, 6, p. 607-609 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Multigene Family
  • Polyketides
  • Complement Activation

• Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae

  Shi, S., Choi, Y. W., Zhao, H., Tan, M. H. & Ang, E. L., Dec 2017, In: Bioresource Technology. 245, p. 1343-1351 9 p.

  Research output: Contribution to journal › Article › peer-review

  • 1-Butanol
  • Butenes
  • Biosensors
  • Yeast
  • metabolite

• Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring

  Freestone, T. S., Ju, K. S., Wang, B. & Zhao, H., Feb 17 2017, In: ACS synthetic biology. 6, 2, p. 217-223 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Phosphonoacetic Acid
  • Multigene Family
  • Biological Products
  • Acids
  • Genes

• Engineering biological systems using automated biofoundries

  Chao, R., Mishra, S., Si, T. & Zhao, H., Jul 2017, In: Metabolic Engineering. 42, p. 98-108 11 p.

  Research output: Contribution to journal › Review article › peer-review

  • Microbial Genetics
  • Bioengineering
  • Biotechnology
  • Biological systems
  • Foundries

• Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry

  Chao, R., Liang, J., Tasan, I., Si, T., Ju, L. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 678-685 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Transcription
  • Foundries
  • Genes
  • DNA

• Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

  Abil, Z., Gumy, L. F., Zhao, H. & Hoogenraad, C. C., Jun 16 2017, In: ACS synthetic biology. 6, 6, p. 950-956 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Carrier Proteins
  • Messenger RNA
  • RNA
  • RNA-Binding Proteins
  • Ligands

• Metabolic engineering of oleaginous yeasts for production of fuels and chemicals

  Shi, S. & Zhao, H., Nov 8 2017, In: Frontiers in Microbiology. 8, NOV, 2185.

  Research output: Contribution to journal › Review article › peer-review

  • Metabolic Engineering
  • Yeasts
  • Genetic Engineering
  • Lipomyces
  • Yarrowia

• Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators

  Bao, Z., Jain, S., Jaroenpuntaruk, V. & Zhao, H., Apr 21 2017, In: ACS synthetic biology. 6, 4, p. 686-693 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Cells
  • Genes
  • Gene Regulatory Networks
  • Activator Appliances

• Probing the molecular determinants of fluorinase specificity

  Yeo, W. L., Chew, X., Smith, D. J., Chan, K. P., Sun, H., Zhao, H., Lim, Y. H. & Ang, E. L., 2017, In: Chemical Communications. 53, 17, p. 2559-2562 4 p.

  Research output: Contribution to journal › Article › peer-review

  • fluorinase
  • 5'-chloro-5'-deoxyadenosine
  • Substrates

• Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

  Si, T., Li, B., Comi, T. J., Wu, Y., Hu, P., Wu, Y., Min, Y., Mitchell, D. A., Zhao, H. & Sweedler, J. V., Sep 13 2017, In: Journal of the American Chemical Society. 139, 36, p. 12466-12473 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Ionization
  • Mass spectrometry
  • Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
  • Desorption
  • ionization

• Programmable DNA-Guided Artificial Restriction Enzymes

  Enghiad, B. & Zhao, H., May 19 2017, In: ACS synthetic biology. 6, 5, p. 752-757 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Enzymes
  • DNA
  • Pyrococcus furiosus
  • Genetic engineering
  • Cloning

• Strain development by whole-cell directed evolution

  Si, T., Lian, J. & Zhao, H., Jan 1 2017, Directed Enzyme Evolution: Advances and Applications. Springer International Publishing, p. 173-200 28 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Multifactorial Inheritance
  • Phenotype
  • Workflow
  • Screening
  • Mutation

• SynV and SynX: A story more than DNA synthesis

  Ren, H. & Zhao, H., May 1 2017, In: Science China Life Sciences. 60, 5, p. 558-560 3 p.

  Research output: Contribution to journal › Article › peer-review

• Targeting Specificity of the CRISPR/Cas9 System

  Tasan, I. & Zhao, H., Sep 15 2017, In: ACS synthetic biology. 6, 9, p. 1609-1613 5 p.

  Research output: Contribution to journal › Review article › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Genes
  • Mutagenesis
  • DNA
  • Target tracking

• Twin-primer non-enzymatic DNA assembly: An efficient and accurate multi-part DNA assembly method

  Liang, J., Liu, Z., Low, X. Z., Ang, E. L. & Zhao, H., Jun 1 2017, In: Nucleic acids research. 45, 11, p. e94

  Research output: Contribution to journal › Article › peer-review

  • Plasmids
  • Automation
  • DNA
  • Synthetic Biology
  • Workflow