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• 2018

  Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system

  Fine, J. D., Shpigler, H. Y., Ray, A. M., Beach, N. J., Sankey, A. L., Cash-Ahmed, A., Huang, Z. Y., Astrauskaite, I., Chao, R., Zhao, H. & Robinson, G. E., Sep 2018, In: PloS one. 13, 9, e0203444.

  Research output: Contribution to journal › Article › peer-review

  • queen honey bees
  • Bees
  • Honey
  • Nutrition
  • Pollen

• Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli

  Ren, H., Biswas, S., Ho, S., Van Der Donk, W. A. & Zhao, H., Oct 19 2018, In: ACS chemical biology. 13, 10, p. 2966-2972 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Bacteriocins
  • Escherichia coli
  • enterocin
  • Genes
  • Glycosylation

• Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli

  Si, T., Tian, Q., Min, Y., Zhang, L., Sweedler, J. V., Van Der Donk, W. A. & Zhao, H., Sep 26 2018, In: Journal of the American Chemical Society. 140, 38, p. 11884-11888 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Protein Sorting Signals
  • Escherichia coli
  • peptide
  • Screening
  • screening

• Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications

  Lian, J., Mishra, S. & Zhao, H., Nov 2018, In: Metabolic Engineering. 50, p. 85-108 24 p.

  Research output: Contribution to journal › Review article › peer-review

  • Metabolic engineering
  • Metabolic Engineering
  • Yeast
  • Saccharomyces cerevisiae
  • Genes

• Recent developments in the application of P450 based biocatalysts

  Wei, Y., Ang, E. L. & Zhao, H., Apr 2018, In: Current Opinion in Chemical Biology. 43, p. 1-7 7 p.

  Research output: Contribution to journal › Review article › peer-review

  • Mixed Function Oxygenases
  • Enzymes
  • Biosynthesis
  • Biological Products
  • Cytochrome P-450 Enzyme System

• RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

  HamediRad, M., Lian, J., Li, H. & Zhao, H., Jun 2018, In: Biotechnology and bioengineering. 115, 6, p. 1552-1560 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Yeast
  • RNA Interference
  • Genes
  • Yeasts

• Synthetic biology advances and applications in the biotechnology industry: a perspective

  Katz, L., Chen, Y. Y., Gonzalez, R., Peterson, T. C., Zhao, H. & Baltz, R. H., Jul 1 2018, In: Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 449-461 13 p.

  Research output: Contribution to journal › Article › peer-review

  • Synthetic biology
  • Synthetic Biology
  • Biotechnology
  • Genetic engineering
  • Industry

• Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding

  Wang, P., Liang, J., Shi, L. Z., Wang, Y., Zhang, P., Ouyang, M., Preece, D., Peng, Q., Shao, L., Fan, J., Sun, J., Li, S. S., Berns, M. W., Zhao, H. & Wang, Y., Sep 19 2018, In: ACS Photonics. 5, 9, p. 3565-3574 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Forster resonance energy transfer
  • Biosensors
  • Energy Transfer
  • bioinstrumentation
  • cells
• 2019

  A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

  Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In: Metabolic Engineering Communications. 9, e00101.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Genomics
  • Genes
  • Yeasts
  • Basidiomycota
  • Genome

• A continuing career in biocatalysis: Frances H. Arnold

  Fasan, R., Jennifer Kan, S. B. & Zhao, H., Nov 1 2019, In: ACS Catalysis. 9, 11, p. 9775-9788 14 p.

  Research output: Contribution to journal › Article › peer-review

  • Biocatalysis
  • Enzymes
  • Biocatalysts
  • Catalysis

• Activation of silent biosynthetic gene clusters using transcription factor decoys

  Wang, B., Guo, F., Dong, S. H. & Zhao, H., Feb 1 2019, In: Nature chemical biology. 15, 2, p. 111-114 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Multigene Family
  • Oxazoles
  • non-ribosomal peptide synthase
  • Polyketide Synthases
  • Transcription Factors

• A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium

  Xing, M., Wei, Y., Hua, G., Li, M., Nanjaraj Urs, A. N., Wang, F., Hu, Y., Zhai, W., Liu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Aug 15 2019, In: Biochemical Journal. 476, 15, p. 2271-2279 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Taurine
  • Sulfur
  • Bacteria
  • Multigene Family
  • Genes

• An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

  Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In: Nature chemical biology. 16, 4, p. 387-390 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Knock-In Techniques
  • Ribonucleoproteins
  • DNA
  • HEK293 Cells
  • Neoplastic Stem Cells

• An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine

  Yin, J., Wei, Y., Liu, D., Hu, Y., Lu, Q., Lui Ang, E., Zhao, H. & Zhang, Y., Oct 25 2019, In: Journal of Biological Chemistry. 294, 43, p. 15662-15671 10 p.

  Research output: Contribution to journal › Article › peer-review

  • pyrimidine
  • Alanine
  • Nitrogen
  • Degradation
  • Bacteria

• A pathway for isethionate dissimilation in Bacillus krulwichiae

  Tong, Y., Wei, Y., Hu, Y., Ang, E. L., Zhao, H. & Zhang, Y., 2019, In: Applied and environmental microbiology. 85, 15, e00793-19.

  Research output: Contribution to journal › Article › peer-review

  • sulfoacetaldehyde
  • Bacillus (bacteria)
  • Bacillus
  • Bacteria
  • bacterium

• Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca

  Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Biochemical Journal. 476, 4, p. 733-746 14 p.

  Research output: Contribution to journal › Article › peer-review

  • sulfoacetaldehyde
  • Short Chain Dehydrogenase-Reductases
  • Klebsiella oxytoca
  • Taurine
  • NADP

• Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense

  Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In: Biochemical Journal. 476, 11, p. 1605-1619 15 p.

  Research output: Contribution to journal › Article › peer-review

  • Ketoglutaric Acids
  • Bifidobacterium
  • Pyridoxal
  • Taurine
  • Pyridoxal Phosphate

• Building a global alliance of biofoundries

  Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., Dec 1 2019, In: Nature communications. 10, 1, 2040.

  Research output: Contribution to journal › Comment/debate › peer-review

  Open Access

  • biotechnology
  • Biotechnology
  • organisms
  • Testing

• Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes

  Yeo, W. L., Heng, E., Tan, L. L., Lim, Y. W., Lim, Y. H., Hoon, S., Zhao, H., Zhang, M. M. & Wong, F. T., 2019, In: Biotechnology and bioengineering. 116, 9, p. 2330-2338 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Editing
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Proteins
  • Genes
  • CRISPR-Cas Systems

• Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098

  Nguyen, K., DeSieno, M. A., Bae, B., Johannes, T. W., Cobb, R. E., Zhao, H. & Nair, S. K., 2019, In: Organic and Biomolecular Chemistry. 17, 6, p. 1506-1518 13 p.

  Research output: Contribution to journal › Article › peer-review

  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid
  • Biosynthesis
  • dimethylaniline monooxygenase (N-oxide forming)
  • biosynthesis
  • 4,6-dinitro-o-cresol

• Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

  Wen, F., Smith, M. R. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 213-234 22 p. (Methods in Molecular Biology; vol. 2024).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Peptide Library
  • T-Lymphocyte Epitopes
  • Major Histocompatibility Complex
  • Yeasts
  • Peptides

• Delta integration CRISPR-Cas (Di-CRISPR) in saccharomyces cerevisiae

  Shi, S., Liang, Y., Ang, E. L. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 73-91 19 p. (Methods in Molecular Biology; vol. 1927).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Saccharomyces cerevisiae
  • Genetic Engineering
  • Workflow
  • Plasmids

• Development of a CRISPR/Cas9-based tool for gene deletion in Issatchenkia orientalis

  Tran, V. G., Cao, M., Fatma, Z., Song, X. & Zhao, H., May 1 2019, In: mSphere. 4, 3, e00345-19.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Gene Deletion
  • Metabolic Engineering
  • Gene Editing
  • Plasmids

• Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides

  Schultz, J. C., Cao, M. & Zhao, H., Aug 2019, In: Biotechnology and bioengineering. 116, 8, p. 2103-2109 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Knockout Techniques
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Gene Deletion
  • Genes
  • Yeasts

• Highly Efficient Single-Pot Scarless Golden Gate Assembly

  Hamedirad, M., Weisberg, S., Chao, R., Lian, J. & Zhao, H., May 17 2019, In: ACS synthetic biology. 8, 5, p. 1047-1054 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Software Design
  • Point Mutation
  • Cicatrix
  • DNA
  • Software design

• Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense

  Zhou, Y., Wei, Y., Nanjaraj Urs, A. N., Lin, L., Xu, T., Hu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In: Bioscience Reports. 39, 6, Y.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • sulfoacetaldehyde
  • Bifidobacterium
  • Taurine
  • Bacteria
  • Oxidoreductases

• Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

  Lian, J., Schultz, C., Cao, M., HamediRad, M. & Zhao, H., Dec 1 2019, In: Nature communications. 10, 1, 5794.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • genome
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • functionals
  • Genes
  • Throughput

• Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria

  Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., N. Nanjaraj Urs, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Dec 1 2019, In: Nature communications. 10, 1, 1609.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • sulfonates
  • Anaerobic Bacteria
  • Bacteria
  • bacteria
  • cleavage

• Towards a fully automated algorithm driven platform for biosystems design

  HamediRad, M., Chao, R., Weisberg, S., Lian, J., Sinha, S. & Zhao, H., Nov 13 2019, In: Nature communications. 10, 1, 5150.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Robotics
  • platforms
  • Lycopene
  • Biosynthetic Pathways
  • Machine Learning