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• 2015

  Direct observation of TALE protein dynamics reveals a two-state search mechanism

  Cuculis, L., Abil, Z., Zhao, H. & Schroeder, C. M., Jun 1 2015, In: Nature communications. 6, 7277.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effectors
  • effectors
  • proteins
  • Observation
  • deoxyribonucleic acid

• DNA-Based Storage: Trends and Methods

  Yazdi, S. M. H. T., Kiah, H. M., Garcia-Ruiz, E., Ma, J., Zhao, H. & Milenkovic, O., Sep 2015, In: IEEE Transactions on Molecular, Biological, and Multi-Scale Communications. 1, 3, p. 230-248 19 p., 7423733.

  Research output: Contribution to journal › Article › peer-review

  • DNA
  • Trends
  • Storage System
  • Archives
  • Random Access

• Editorial overview: Opportunities and challenges in synthetic biology

  Chang, M. C. Y. & Zhao, H., Oct 1 2015, In: Current Opinion in Chemical Biology. 28, p. 5-6 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Engineered biosynthesis of natural products in heterologous hosts

  Luo, Y., Li, B. Z., Liu, D., Zhang, L., Chen, Y., Jia, B., Zeng, B. X., Zhao, H. & Yuan, Y. J., Aug 7 2015, In: Chemical Society Reviews. 44, 15, p. 5265-5290 26 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthesis
  • Biological Products
  • Genes
  • Polyketides
  • Terpenes

• Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome

  Abil, Z. & Zhao, H., Jul 6 2015, In: Molecular BioSystems. 11, 10, p. 2658-2665 8 p.

  Research output: Contribution to journal › Article › peer-review

  • RNA-Binding Proteins
  • Transcriptome
  • Protein Engineering
  • RNA
  • RNA-Binding Motifs

• High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

  Cobb, R. E., Wang, Y. & Zhao, H., Jun 19 2015, In: ACS synthetic biology. 4, 6, p. 723-728 6 p.

  Research output: Contribution to journal › Article › peer-review

  • CRISPR-Cas Systems
  • Gene Editing
  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Genes

• High throughput screening and selection methods for directed enzyme evolution

  Xiao, H., Bao, Z. & Zhao, H., Apr 29 2015, In: Industrial and Engineering Chemistry Research. 54, 16, p. 4011-4020 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Enzymes
  • Screening
  • Throughput
  • Costs

• Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., May 15 2015, In: ACS synthetic biology. 4, 5, p. 585-594 10 p.

  Research output: Contribution to journal › Article › peer-review

  • CRISPR-Associated Proteins
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • Proteins

• Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB)

  Zhao, H., Jan 1 2015, Synthetic Biology: Engineering, Evolution, and Design Conference 2015, SEED 2015. AIChE, p. 784-785 2 p. (Synthetic Biology: Engineering, Evolution, and Design Conference 2015, SEED 2015; vol. 2).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Improving and repurposing biocatalysts via directed evolution

  Denard, C. A., Ren, H. & Zhao, H., Apr 1 2015, In: Current Opinion in Chemical Biology. 25, p. 55-64 10 p.

  Research output: Contribution to journal › Review article › peer-review

  • Enzymes
  • Stereoselectivity
  • Biocatalysis
  • Chemical reactivity
  • Protein Engineering

• Industrial biotechnology: Tools and applications

  Zhao, H. & Tan, T., Nov 15 2015, In: Biotechnology Advances. 33, 7, p. 1393-1394 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• In Situ Assembly of Antifouling/Bacterial Silver Nanoparticle-Hydrogel Composites with Controlled Particle Release and Matrix Softening

  Baek, K., Liang, J., Lim, W. T., Zhao, H., Kim, D. H. & Kong, H., Jul 22 2015, In: ACS Applied Materials and Interfaces. 7, 28, p. 15359-15367 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Silver nanoparticles
  • Hydrogels
  • Polyethylene Glycol Dimethacrylate Hydrogel
  • Gels
  • Composite materials

• Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production

  Feng, X., Lian, J. & Zhao, H., Jan 1 2015, In: Metabolic Engineering. 27, p. 10-19 10 p.

  Research output: Contribution to journal › Article › peer-review

  • cetyl alcohol
  • Metabolic engineering
  • Metabolic Engineering
  • Yeast
  • Saccharomyces cerevisiae

• Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae

  Eriksen, D. T., HamediRad, M., Yuan, Y. & Zhao, H., Jul 17 2015, In: ACS synthetic biology. 4, 7, p. 808-814 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthetic Pathways
  • Fatty acids
  • Yeast
  • Esters
  • Saccharomyces cerevisiae

• Production of long chain alcohols and alkanes upon coexpression of an acyl-ACP reductase and aldehyde-deformylating oxygenase with a bacterial type-I fatty acid synthase in E. coli

  Coursolle, D., Lian, J., Shanklin, J. & Zhao, H., Jun 17 2015, In: Molecular BioSystems. 11, 9, p. 2464-2472 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Type I Fatty Acid Synthase
  • Alkanes
  • Oxygenases
  • Aldehyde Reductase
  • Alcohols

• Rapid prototyping of microbial cell factories via genome-scale engineering

  Si, T., Xiao, H. & Zhao, H., Nov 15 2015, In: Biotechnology Advances. 33, 7, p. 1420-1432 13 p.

  Research output: Contribution to journal › Review article › peer-review

  • Rapid prototyping
  • Industrial plants
  • Genes
  • Genome
  • Nucleotides

• Recent advances in combinatorial biosynthesis for drug discovery

  Sun, H., Liu, Z., Zhao, H. & Ang, E. L., Feb 12 2015, In: Drug Design, Development and Therapy. 9, p. 823-833 11 p.

  Research output: Contribution to journal › Review article › peer-review

  • Drug Discovery
  • Biological Products
  • Biosynthetic Pathways
  • Biodiversity
  • Pharmaceutical Preparations

• Recent advances in DNA assembly technologies

  Chao, R., Yuan, Y. & Zhao, H., 2015, In: FEMS yeast research. 15, 1, 12171.

  Research output: Contribution to journal › Short survey › peer-review

  • Technology
  • DNA
  • Synthetic Biology
  • Metabolic Engineering
  • Digestion

• Regulatory RNA-assisted genome engineering in microorganisms

  Si, T., HamediRad, M. & Zhao, H., Dec 1 2015, In: Current Opinion in Biotechnology. 36, p. 85-90 6 p.

  Research output: Contribution to journal › Review article › peer-review

  • RNA
  • Microorganisms
  • Genes
  • Genome
  • Metabolic engineering

• Reversal of the β-oxidation cycle in saccharomyces cerevisiae for production of fuels and chemicals

  Lian, J. & Zhao, H., Mar 20 2015, In: ACS synthetic biology. 4, 3, p. 332-341 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Yeast
  • Saccharomyces cerevisiae
  • Oxidation
  • Fatty Acids
  • 1-Butanol

• RNAi-assisted genome evolution in saccharomyces cerevisiae for complex phenotype engineering

  Si, T., Luo, Y., Bao, Z. & Zhao, H., Mar 20 2015, In: ACS synthetic biology. 4, 3, p. 283-291 9 p.

  Research output: Contribution to journal › Article › peer-review

  • RNA
  • Yeast
  • RNA Interference
  • Saccharomyces cerevisiae
  • Genes

• Selective elimination of mitochondrial mutations in the germline by genome editing

  Reddy, P., Ocampo, A., Suzuki, K., Luo, J., Bacman, S. R., Williams, S. L., Sugawara, A., Okamura, D., Tsunekawa, Y., Wu, J., Lam, D., Xiong, X., Montserrat, N., Esteban, C. R., Liu, G. H., Sancho-Martinez, I., Manau, D., Civico, S., Cardellach, F., Del Mar O'Callaghan, M. & 5 others, Campistol, J., Zhao, H., Campistol, J. M., Moraes, C. T. & Izpisua Belmonte, J. C., Apr 23 2015, In: Cell. 161, 3, p. 459-469 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Editing
  • Germ-Line Mutation
  • Mitochondrial DNA
  • Genes
  • Mitochondrial Diseases

• Structure-guided design and biosynthesis of a novel FR-900098 analogue as a potent Plasmodium falciparum 1-deoxy-d-xylulose-5-phosphate reductoisomerase (Dxr) inhibitor

  Cobb, R. E., Bae, B., Li, Z., Desieno, M. A., Nair, S. K. & Zhao, H., Feb 14 2015, In: Chemical Communications. 51, 13, p. 2526-2528 3 p.

  Research output: Contribution to journal › Article › peer-review

  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid
  • xylulose-5-phosphate
  • Biosynthesis
  • Phosphates
  • Antimalarials

• Synthetic biology for therapeutic applications

  Abil, Z., Xiong, X. & Zhao, H., Feb 2 2015, In: Molecular pharmaceutics. 12, 2, p. 322-331 10 p.

  Research output: Contribution to journal › Review article › peer-review

  • Synthetic Biology
  • Bacterial Drug Resistance
  • Drug Discovery
  • Communicable Diseases
  • Vaccines

• Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus

  Luo, Y., Zhang, L., Barton, K. W. & Zhao, H., Apr 29 2015, In: ACS synthetic biology. 4, 9, p. 1001-1010 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Actinobacteria
  • Streptomyces
  • Biological Products
  • Biosynthetic Pathways
  • Gene encoding
• 2016

  A brief overview of synthetic biology research programs and roadmap studies in the United States

  Si, T. & Zhao, H., Dec 1 2016, In: Synthetic and Systems Biotechnology. 1, 4, p. 258-264 7 p.

  Research output: Contribution to journal › Review article › peer-review

  • Synthetic biology
  • Synthetic Biology
  • Government Agencies
  • Biological systems

• Accelerated genome engineering through multiplexing

  Bao, Z., Cobb, R. E. & Zhao, H., Jan 1 2016, In: Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 8, 1, p. 5-21 17 p.

  Research output: Contribution to journal › Review article › peer-review

  • Multiplexing
  • Genes
  • Genome
  • Bioengineering
  • Gene Editing

• ACS Catalysis and the Scope of Papers Sought in Three Catalysis Subdisciplines: Biocatalysis and Enzymology, Molecular Catalysis for Organic Synthesis, and Heterogeneous Photocatalysis

  Chang, S., Fornasiero, P., Gunnoe, T. B., Jones, C. W., Linic, S., Ooi, T., Williams, R. M. & Zhao, H., Jul 1 2016, In: ACS Catalysis. 6, 7, p. 4782-4785 4 p.

  Research output: Contribution to journal › Review article › peer-review

• A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

  Shi, S., Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., Jan 1 2016, In: Metabolic Engineering. 33, p. 19-27 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • 2,3-butylene glycol
  • Xylose

• A step through the looking glass

  Liang, Y., Ang, E. L. & Zhao, H., Sep 1 2016, In: Science China Life Sciences. 59, 9, p. 979-980 2 p.

  Research output: Contribution to journal › Article › peer-review

• Biocatalysis for Drug Discovery and Development

  Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., May 13 2016, Green Biocatalysis. Wiley, p. 421-455 35 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Biocatalysis
  • Drug Discovery
  • Drug products
  • Enzymes
  • Biocatalysts

• Characterization of bacillus subtilis colony biofilms via mass spectrometry and fluorescence imaging

  Si, T., Li, B., Zhang, K., Xu, Y., Zhao, H. & Sweedler, J. V., Jun 3 2016, In: Journal of Proteome Research. 15, 6, p. 1955-1962 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Fluorescence imaging
  • Bacilli
  • Biofilms
  • Mass spectrometry
  • Optical Imaging

• Combinatorial pathway engineering for optimized production of the anti-malarial FR900098

  Freestone, T. S. & Zhao, H., Feb 1 2016, In: Biotechnology and bioengineering. 113, 2, p. 384-392 9 p.

  Research output: Contribution to journal › Article › peer-review

  • 3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid
  • Antimalarials
  • Libraries
  • Screening
  • Organophosphonates

• Combined and iterative use of computational design and directed evolution for protein-ligand binding design

  Wang, M. & Zhao, H., May 1 2016, Methods in Molecular Biology. Humana Press Inc., p. 139-153 15 p. (Methods in Molecular Biology; vol. 1414).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Protein Binding
  • Switch Genes
  • Ligands
  • Two-Hybrid System Techniques
  • Mutagenesis

• Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

  Lian, J., Jin, R. & Zhao, H., Nov 1 2016, In: Biotechnology and bioengineering. 113, 11, p. 2462-2473 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Antibiotics
  • Yeast
  • Saccharomyces cerevisiae
  • Genes
  • Butenes

• CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

  Wendt, K. E., Ungerer, J., Cobb, R. E., Zhao, H. & Pakrasi, H. B., Jun 23 2016, In: Microbial cell factories. 15, 1, 115.

  Research output: Contribution to journal › Article › peer-review

  • Cyanobacteria
  • Mutagenesis
  • Synechococcus
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • CRISPR-Associated Protein 9

• Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae

  Wang, M., Li, S. & Zhao, H., Jan 1 2016, In: Biotechnology and bioengineering. 113, 1, p. 206-215 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Metabolites
  • Yeast
  • Gene Regulatory Networks
  • Gene expression

• Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate

  Sun, H., Yeo, W. L., Lim, Y. H., Chew, X., Smith, D. J., Xue, B., Chan, K. P., Robinson, R. C., Robins, E. G., Zhao, H. & Ang, E. L., Jan 1 2016, In: Angewandte Chemie - International Edition. 55, 46, p. 14277-14280 4 p.

  Research output: Contribution to journal › Article › peer-review

  • 5'-fluoro-5'-deoxyadenosine
  • fluorinase
  • 5'-chloro-5'-deoxyadenosine
  • Fluorination
  • Methionine

• Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization

  Wang, M., Yu, C. & Zhao, H., Mar 1 2016, In: Biotechnology and bioengineering. 113, 3, p. 484-491 8 p.

  Research output: Contribution to journal › Article › peer-review

  • xylose-glucose
  • Xylose
  • Glucose
  • Neurospora crassa
  • Biofuels

• Editorial: Synthetic biology and bioinformatics

  Medema, M. H. & Zhao, H., Aug 2016, In: Natural Product Reports. 33, 8, p. 913-914 2 p.

  Research output: Contribution to journal › Editorial › peer-review

• Engineered minicellulosomes for consolidated bioprocessing

  Liang, Y., Ang, E. L. & Zhao, H., Jul 11 2016, Handbook of Carbohydrate-Modifying Biocatalysts. Pan Stanford Publishing Pte. Ltd., Vol. 2. p. 965-992 28 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Engineering microbial hosts for production of bacterial natural products

  Zhang, M. M., Wang, Y., Ang, E. L. & Zhao, H., Aug 2016, In: Natural Product Reports. 33, 8, p. 963-987 25 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biological Products
  • Actinobacteria
  • Genetic Engineering
  • Genetic engineering
  • Fermentation

• Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

  Yuan, Y., Andersen, E. & Zhao, H., Jan 15 2016, In: ACS synthetic biology. 5, 1, p. 46-52 7 p.

  Research output: Contribution to journal › Article › peer-review

  • DNA
  • Plasmids
  • Yeast
  • Ligases
  • Networks (circuits)

• Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering

  Lian, J. & Zhao, H., Jul 15 2016, In: ACS synthetic biology. 5, 7, p. 689-697 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Oxidoreductases
  • Lipoylation
  • Yeast
  • Pyruvic Acid
  • Cytosol

• High-Efficiency Genome Editing of Streptomyces Species by an Engineered CRISPR/Cas System

  Wang, Y., Cobb, R. E. & Zhao, H., 2016, Methods in Enzymology. O'Connor, S. E. (ed.). Academic Press Inc., p. 271-284 14 p. (Methods in Enzymology; vol. 575).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • CRISPR-Cas Systems
  • Gene Editing
  • Streptomyces
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Genes

• High-throughput screening or selection methods for evolutionary enzyme engineering

  Shi, S., Zhang, H., Ang, E. L. & Zhao, H., May 17 2016, Understanding Enzymes: Function, Design, Engineering and Analysis. Pan Stanford Publishing Pte. Ltd., p. 707-744 38 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Identification and engineering of n-butanol biosensors by transcriptome analysis in yeast

  Shi, S., Ang, E. L., Tan, M. H. & Zhao, H., Jan 1 2016, Metabolic Engineering 11. AIChE, p. 458-459 2 p. (Metabolic Engineering 11).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Identification of an important motif that controls the activity and specificity of sugar transporters

  Wang, M., Yu, C. & Zhao, H., Jul 1 2016, In: Biotechnology and bioengineering. 113, 7, p. 1460-1467 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • Sugars
  • Glucose
  • xylose-glucose
  • Facilitative Glucose Transport Proteins

• Members of the rusc protein family interact with Sufu and inhibit vertebrate hedgehog signaling

  Jin, Z., Schwend, T., Fu, J., Bao, Z., Liang, J., Zhao, H., Mei, W. & Yang, J., Nov 1 2016, In: Development (Cambridge). 143, 21, p. 3944-3955 12 p.

  Research output: Contribution to journal › Article › peer-review

  • Hedgehogs
  • vertebrate
  • Vertebrates
  • family
  • protein

• Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae

  Shi, S., Si, T., Liu, Z., Zhang, H., Ang, E. L. & Zhao, H., May 10 2016, In: Scientific reports. 6, 25675.

  Research output: Contribution to journal › Article › peer-review

  • 1-Butanol
  • Metabolic Engineering
  • Saccharomyces cerevisiae
  • citramalate
  • Threonine