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• 2013

  Introduction

  Zhao, H., Aug 27 2013, In: Synthetic Biology.

  Research output: Contribution to journal › Article › peer-review

• Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via ¹³C metabolic flux analysis

  Feng, X. & Zhao, H., Sep 1 2013, In: AIChE Journal. 59, 9, p. 3195-3202 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Flux Analysis
  • xylose-glucose
  • Xylose
  • Metabolism
  • Yeast

• Investigating host dependence of xylose utilization in recombinant Saccharomyces cerevisiae strains using RNA-seq analysis

  Feng, X. & Zhao, H., 2013, In: Biotechnology for Biofuels. 6, 1, 96.

  Research output: Contribution to journal › Article › peer-review

  • Xylose
  • RNA
  • Yeast
  • Saccharomyces cerevisiae
  • regulation

• Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via ¹³C metabolic flux analysis

  Feng, X. & Zhao, H., Nov 18 2013, In: Microbial cell factories. 12, 1, 114.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Flux Analysis
  • Xylose
  • Metabolism
  • Yeast
  • Saccharomyces cerevisiae

• Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

  Li, S., Ha, S. J., Kim, H. J., Galazka, J. M., Cate, J. H. D., Jin, Y. S. & Zhao, H., Oct 10 2013, In: Journal of Biotechnology. 168, 1, p. 1-6 6 p.

  Research output: Contribution to journal › Article › peer-review

  • aldose 1-epimerase
  • Cellobiose
  • Cell growth
  • Yeast
  • Glucose

• Modular assembly of designer Puf proteins for specific post-transcriptional regulation of endogenous RNA

  Abil, Z. & Zhao, H., Jan 1 2013, Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Multistep one-pot reactions combining biocatalysts and chemical catalysts for asymmetric synthesis

  Denard, C. A., Hartwig, J. F. & Zhao, H., Dec 6 2013, In: ACS Catalysis. 3, 12, p. 2856-2864 9 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biocatalysts
  • Catalysts
  • Stereoselectivity
  • Regioselectivity
  • Biosynthetic Pathways

• Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands

  Liang, J., Mclachlan, M. J. & Zhao, H., May 1 2013, In: Biotechnology and bioengineering. 110, 5, p. 1419-1429 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Switch Genes
  • Artificial Cells
  • Ligands
  • Gene expression
  • Cells

• Pathway engineering as an enabling synthetic biology tool

  Eriksen, D. T., Li, S. & Zhao, H., Aug 27 2013, Synthetic Biology. Elsevier Inc., p. 43-61 19 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Synthetic biology
  • Synthetic Biology
  • Metabolic Networks and Pathways
  • 1,4-butanediol
  • Glucaric Acid

• Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold

  Shao, Z., Rao, G., Li, C., Abil, Z., Luo, Y. & Zhao, H., Nov 15 2013, In: ACS synthetic biology. 2, 11, p. 662-669 8 p.

  Research output: Contribution to journal › Article › peer-review

  • spectinabilin
  • Biosynthetic Pathways
  • Synthetic biology
  • Metabolites
  • Bioactivity

• Synthetic Biology

  Zhao, H., Jan 1 2013, Elsevier Inc.

  Research output: Book/Report/Conference proceeding › Book

  • Synthetic biology
  • Synthetic Biology
  • Artificial photosynthesis
  • Biological systems
  • Photosynthesis

• Transcription activator-like effector nucleases (TALENs): a highly efficient and versatile tool for genome editing.

  Sun, N. & Zhao, H., Jul 2013, In: Biotechnology and bioengineering. 110, 7, p. 1811-1821 11 p.

  Research output: Contribution to journal › Review article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Gene Editing
  • Transcription
  • Genes
  • Transcription Activator-Like Effectors
• 2014

  Aryl-aldehyde formation in fungal polyketides: Discovery and characterization of a distinct biosynthetic mechanism

  Wang, M., Beissner, M. & Zhao, H., Feb 20 2014, In: Chemistry and Biology. 21, 2, p. 257-263 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Polyketide Synthases
  • Polyketides
  • Aldehydes
  • non-ribosomal peptide synthase
  • Aspergillus

• A single-chain TALEN architecture for genome engineering

  Sun, N. & Zhao, H., Mar 1 2014, In: Molecular BioSystems. 10, 3, p. 446-453 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Genome
  • DNA Cleavage
  • Peptides
  • Deoxyribonuclease I

• A synthetic device for intracellular mRNA transport and local protein translation in eukaryotes

  Abil, Z., Hoogenraad, C. & Zhao, H., 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, Vol. 1. p. 104-113 10 p.

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Carrier Proteins
  • RNA transport
  • Messenger RNA
  • Protein Biosynthesis
  • transport proteins

• A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases

  Sun, N. & Zhao, H., 2014, Homing Endonucleases: Methods and Protocols. Humana Press Inc., p. 87-96 10 p. (Methods in Molecular Biology; vol. 1123).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Endonucleases
  • Plasmids
  • DNA Cleavage
  • Double-Stranded DNA Breaks
  • Cell Survival

• Characterization and engineering of the adenylation domain of a NRPS-like protein: A potential biocatalyst for aldehyde generation

  Wang, M. & Zhao, H., Apr 4 2014, In: ACS Catalysis. 4, 4, p. 1219-1225 7 p.

  Research output: Contribution to journal › Article › peer-review

  • non-ribosomal peptide synthase
  • Biocatalysts
  • Aldehydes
  • Peptides
  • Staphylococcal Protein A

• Characterization of the N-oxygenase AurF from Streptomyces thioletus

  Chanco, E., Choi, Y. S., Sun, N., Vu, M. & Zhao, H., Oct 15 2014, In: Bioorganic and Medicinal Chemistry. 22, 20, p. 5569-5577 9 p.

  Research output: Contribution to journal › Article › peer-review

  • 4-nitrobenzoic acid
  • Methylphenazonium Methosulfate
  • Oxygenases
  • Streptomyces
  • aureothin

• Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in d-tagatose production

  Jagtap, S. S., Singh, R., Kang, Y. C., Zhao, H. & Lee, J. K., May 10 2014, In: Enzyme and Microbial Technology. 58-59, p. 44-51 8 p.

  Research output: Contribution to journal › Article › peer-review

  • galactitol 2-dehydrogenase
  • tagatose
  • Rhizobium
  • Oxidoreductases
  • Cloning

• Comparative biochemical characterization of three exolytic oligoalginate lyases from Vibrio splendidus reveals complementary substrate scope, temperature, and pH adaptations

  Jagtap, S. S., Hehemann, J. H., Polz, M. F., Lee, J. K. & Zhao, H., 2014, In: Applied and environmental microbiology. 80, 14, p. 4207-4214 8 p.

  Research output: Contribution to journal › Article › peer-review

  • poly(beta-D-mannuronate) lyase
  • Vibrio splendidus
  • alginate lyase
  • Vibrio
  • alginate

• Cooperative tandem catalysis by an organometallic complex and a metalloenzyme

  Denard, C. A., Huang, H., Bartlett, M. J., Lu, L., Tan, Y., Zhao, H. & Hartwig, J. F., Jan 7 2014, In: Angewandte Chemie - International Edition. 53, 2, p. 465-469 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Organometallics
  • Catalysis
  • Catalysts
  • Alkenes
  • Olefins

• Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

  Lian, J., Si, T., Nair, N. U. & Zhao, H., 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. American Institute of Chemical Engineers, p. 750-760 11 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 2).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Acetyl Coenzyme A
  • Butenes
  • Yeast
  • 1-Butanol
  • butanol

• Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

  Lian, J., Si, T., Nair, N. U. & Zhao, H., Jul 2014, In: Metabolic Engineering. 24, p. 139-149 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Acetyl Coenzyme A
  • Butenes
  • Yeast
  • 1-Butanol
  • Saccharomyces cerevisiae

• Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions in Saccharomyces cerevisiae

  Lian, J., Li, Y., HamediRad, M. & Zhao, H., Aug 2014, In: Biotechnology and bioengineering. 111, 8, p. 1521-1531 11 p.

  Research output: Contribution to journal › Article › peer-review

  • cellodextrin
  • Cellobiose
  • Biofuels
  • Yeast
  • Fermentation

• Directed evolution of enzymes for industrial biocatalysis

  Liang, Y., Ang, E. L. & Zhao, H., Jan 1 2014, Industrial Biocatalysis. Pan Stanford Publishing Pte. Ltd., p. 73-114 42 p.

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• DNA assembly techniques for next-generation combinatorial biosynthesis of natural products

  Cobb, R. E., Ning, J. C. & Zhao, H., Feb 2014, In: Journal of Industrial Microbiology and Biotechnology. 41, 2, p. 469-477 9 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biosynthesis
  • DNA
  • Biological Products
  • Biosynthetic Pathways
  • Synthetic biology

• Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae

  Liang, Y., Si, T., Ang, E. L. & Zhao, H., Jan 1 2014, In: Applied and environmental microbiology. 80, 21, p. 6677-6684 8 p.

  Research output: Contribution to journal › Article › peer-review

  • ethanol fermentation
  • saccharification
  • Fermentation
  • yeast
  • ethanol

• Engineering chimeric RNA-binding proteins: Modular design, novel function

  Abil, Z., Denard, C. A., Hoogenraad, C. & Zhao, H., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 2).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Exploiting Issatchenkia orientalis SD108 for succinic acid production

  Xiao, H., Shao, Z., Jiang, Y., Dole, S. & Zhao, H., Aug 27 2014, In: Microbial cell factories. 13, 1, 121.

  Research output: Contribution to journal › Review article › peer-review

  • Organic acids
  • Succinic Acid
  • Acids
  • Yeast
  • Toxicity

• FairyTALE: A high-throughput TAL effector synthesis platform

  Liang, J., Chao, R., Abil, Z., Bao, Z. & Zhao, H., Feb 21 2014, In: ACS synthetic biology. 3, 2, p. 67-73 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effectors
  • Transcription
  • Throughput
  • DNA sequences
  • Transcription Activator-Like Effector Nucleases

• Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

  Xiao, H. & Zhao, H., May 23 2014, In: Biotechnology for Biofuels. 7, 1, 78.

  Research output: Contribution to journal › Article › peer-review

  • Furaldehyde
  • SUMO-1 Protein
  • Furfural
  • RNA
  • Yeast

• Homology-integrated CRISPR-cas (HI-CRISPR) system for one-step multigene disruption in saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., Jan 1 2014, Systems Biology 2014 - Topical Conference at the 2014 AIChE Annual Meeting. AIChE, p. 3-12 10 p. (Systems Biology 2014 - Topical Conference at the 2014 AIChE Annual Meeting).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • Saccharomyces cerevisiae
  • Genes
  • Biosynthetic Pathways

• Homology-integrated CRISPR-cas (HI-CRISPR) system for one-step multigene disruption in saccharomyces cerevisiae

  Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, p. 27-36 10 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 1).

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Yeast
  • gene targeting
  • Saccharomyces cerevisiae
  • Genes

• Manipulating natural product biosynthetic pathways via DNA assembler

  Shao, Z. & Zhao, H., 2014, In: Current protocols in chemical biology. 6, 2, p. 65-100 36 p.

  Research output: Contribution to journal › Article › peer-review

  • Biosynthetic Pathways
  • Biological Products
  • spectinabilin
  • DNA
  • Synthetic Biology

• Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol

  Lian, J., Chao, R. & Zhao, H., May 2014, In: Metabolic Engineering. 23, p. 92-99 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Butylene Glycols
  • 2,3-butylene glycol
  • Metabolic engineering
  • Metabolic Engineering
  • Yeast

• Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA

  Abil, Z., Denard, C. A. & Zhao, H., Mar 1 2014, In: Journal of Biological Engineering. 8, 1, 7.

  Research output: Contribution to journal › Article › peer-review

  • Protein Engineering
  • RNA
  • Proteins
  • RNA-Binding Proteins
  • Carrier Proteins

• Preparing your manuscript for submission to ACS catalysis

  Jones, C. W., Smith, D. J., Gunnoe, T. B., Zhao, H., Sautet, P., Scott, S. L. & Xu, B. Q., Jan 1 2014, In: ACS Catalysis. 4, 8, p. 2827-2828 2 p.

  Research output: Contribution to journal › Review article › peer-review

  • Catalysis

• Protein design for pathway engineering

  Eriksen, D. T., Lian, J. & Zhao, H., Feb 2014, In: Journal of Structural Biology. 185, 2, p. 234-242 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Metabolic Engineering
  • Biocatalysis
  • Enzymes
  • Proteins
  • Protein Engineering

• Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites

  Lian, J. & Zhao, H., Jan 1 2014, In: Journal of Industrial Microbiology and Biotechnology. 42, 3, p. 437-451 15 p.

  Research output: Contribution to journal › Review article › peer-review

  • Biosynthesis
  • Metabolites
  • Fatty acids
  • Yeast
  • Saccharomyces cerevisiae

• Recent advances in natural product discovery

  Luo, Y., Cobb, R. E. & Zhao, H., Dec 1 2014, In: Current Opinion in Biotechnology. 30, p. 230-237 8 p.

  Research output: Contribution to journal › Review article › peer-review

  • Genomics
  • Biological Products
  • Bioinformatics
  • Drug products
  • Screening

• Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs

  Sun, N. & Zhao, H., May 2014, In: Biotechnology and bioengineering. 111, 5, p. 1048-1053 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Stem cells
  • Transcription
  • Induced Pluripotent Stem Cells
  • Sickle Cell Anemia

• SunnyTALEN: A second-generation TALEN system for human genome editing

  Sun, N., Bao, Z., Xiong, X. & Zhao, H., Apr 2014, In: Biotechnology and bioengineering. 111, 4, p. 683-691 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription Activator-Like Effector Nucleases
  • Gene Editing
  • Transcription
  • Human Genome
  • Genes

• Tandem catalytic conversion of glucose to 5-hydroxymethylfurfural with an immobilized enzyme and a solid acid

  Huang, H., Denard, C. A., Alamillo, R., Crisci, A. J., Miao, Y., Dumesic, J. A., Scott, S. L. & Zhao, H., Jul 3 2014, In: ACS Catalysis. 4, 7, p. 2165-2168 4 p.

  Research output: Contribution to journal › Article › peer-review

  • 5-hydroxymethylfurfural
  • Immobilized Enzymes
  • Enzymes
  • Glucose
  • Fructose

• Utilizing an endogenous pathway for 1-butanol production in Saccharomyces cerevisiae

  Si, T., Luo, Y., Xiao, H. & Zhao, H., Mar 2014, In: Metabolic Engineering. 22, p. 60-68 9 p.

  Research output: Contribution to journal › Article › peer-review

  • 1-Butanol
  • Butenes
  • Yeast
  • Saccharomyces cerevisiae
  • Alcohols
• 2015

  Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic

  Badur, A. H., Jagtap, S. S., Yalamanchili, G., Lee, J. K., Zhao, H. & Rao, C. V., Jan 1 2015, In: Applied and environmental microbiology. 81, 5, p. 1865-1873 9 p.

  Research output: Contribution to journal › Article › peer-review

  • poly(beta-D-mannuronate) lyase
  • Vibrio splendidus
  • alginate lyase
  • Vibrio
  • alginate

• A Rewritable, Random-Access DNA-Based Storage System

  Tabatabaei Yazdi, S. M. H., Yuan, Y., Ma, J., Zhao, H. & Milenkovic, O., Sep 18 2015, In: Scientific reports. 5, 14138.

  Research output: Contribution to journal › Article › peer-review

  • DNA
  • Information Storage and Retrieval
  • Sensitivity and Specificity

• Building biological foundries for next-generation synthetic biology

  Chao, R., Yuan, Y. B. & Zhao, H. M., Jul 24 2015, In: Science China Life Sciences. 58, 7, p. 658-665 8 p.

  Research output: Contribution to journal › Review article › peer-review

  • Synthetic biology
  • Synthetic Biology
  • synthetic biology
  • Foundries
  • Metabolites

• Chromatin architecture reorganization during stem cell differentiation

  Dixon, J. R., Jung, I., Selvaraj, S., Shen, Y., Antosiewicz-Bourget, J. E., Lee, A. Y., Ye, Z., Kim, A., Rajagopal, N., Xie, W., Diao, Y., Liang, J., Zhao, H., Lobanenkov, V. V., Ecker, J. R., Thomson, J. A. & Ren, B., Feb 19 2015, In: Nature. 518, 7539, p. 331-336 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Chromatin
  • Cell Differentiation
  • Stem Cells
  • Human Embryonic Stem Cells
  • Cell Lineage

• Development of a One-Pot tandem reaction combining ruthenium-catalyzed alkene metathesis and enantioselective enzymatic oxidation to produce Aryl epoxides

  Denard, C. A., Bartlett, M. J., Wang, Y., Lu, L., Hartwig, J. F. & Zhao, H., Jun 5 2015, In: ACS Catalysis. 5, 6, p. 3817-3822 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Ruthenium
  • Alkenes
  • Epoxy Compounds
  • Olefins
  • Enantioselectivity

• Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening

  Li, S., Si, T., Wang, M. & Zhao, H., Dec 18 2015, In: ACS synthetic biology. 4, 12, p. 1308-1315 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Malonyl Coenzyme A
  • Metabolites
  • Yeast
  • Genetic Testing
  • Screening