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2017

Conformational equilibria of light-activated rhodopsin in nanodiscs

Van Eps, N., Caro, L. N., Morizumi, T., Kusnetzow, A. K., Szczepek, M., Hofmann, K. P., Bayburt, T. H., Sligar, S., Ernst, O. P. & Hubbell, W. L., Apr 18 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 16, p. E3268-E3275

Research output: Contribution to journalArticle

Rhodopsin
G-Protein-Coupled Receptors
Light
Electrons
Transducin
2016

Evidence that cytochrome b5 acts as a redox donor in CYP17A1 mediated androgen synthesis

Duggal, R., Liu, Y., Gregory, M. C., Denisov, I. G., Kincaid, J. R. & Sligar, S. G., Aug 19 2016, In : Biochemical and Biophysical Research Communications. 477, 2, p. 202-208 7 p.

Research output: Contribution to journalArticle

Cytochromes b5
Cytochrome P-450 Enzyme System
Androgens
Lyases
Oxidation-Reduction
2018

Human Cytochrome CYP17A1: The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone

Mak, P. J., Duggal, R., Denisov, I. G., Gregory, M. C., Sligar, S. & Kincaid, J. R., Jun 13 2018, In : Journal of the American Chemical Society. 140, 23, p. 7324-7331 8 p.

Research output: Contribution to journalArticle

17-alpha-Hydroxyprogesterone
Pregnenolone
Lyases
androgen
Cytochromes
2019

Nanodiscs as a New Tool to Examine Lipid–Protein Interactions

Denisov, I. G., Schuler, M. A. & Sligar, S., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 645-671 27 p. (Methods in Molecular Biology; vol. 2003).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lipids
Lipoproteins
Proteins
Membranes
Protein Transport
2016

The use of isomeric testosterone dimers to explore allosteric effects in substrate binding to cytochrome P450 CYP3A4

Denisov, I. G., Mak, P. J., Grinkova, Y. V., Bastien, D., Bérubé, G., Sligar, S. G. & Kincaid, J. R., May 1 2016, In : Journal of Inorganic Biochemistry. 158, p. 77-85 9 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
Dimers
Cytochrome P-450 Enzyme System
Testosterone
Substrates
2017

Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria

Guo, Z., Sevrioukova, I. F., Denisov, I. G., Zhang, X., Chiu, T. L., Thomas, D. G., Hanse, E. A., Cuellar, R. A. D., Grinkova, Y. V., Langenfeld, V. W., Swedien, D. S., Stamschror, J. D., Alvarez, J., Luna, F., Galván, A., Bae, Y. K., Wulfkuhle, J. D., Gallagher, R. I., Petricoin, E. F., Norris, B. & 17 others, Flory, C. M., Schumacher, R. J., O'Sullivan, M. G., Cao, Q., Chu, H., Lipscomb, J. D., Atkins, W. M., Gupta, K., Kelekar, A., Blair, I. A., Capdevila, J. H., Falck, J. R., Sligar, S. G., Poulos, T. L., Georg, G. I., Ambrose, E. & Potter, D. A., Oct 19 2017, In : Cell chemical biology. 24, 10, p. 1259-1275.e6

Research output: Contribution to journalArticle

Biguanides
Cytochrome P-450 CYP3A
Mitochondria
Heme
Cytochrome P-450 Enzyme System
2018

SMPL synaptic membranes: Nanodisc-mediated synaptic membrane mimetics expand the toolkit for drug discovery and the molecular cell biology of synapses

Lo Bu, R., Clark, J., DiChiara, T., Sligar, S. G. & Klein, W. L., Jan 1 2018, Neuromethods. Humana Press Inc., p. 227-250 24 p. (Neuromethods; vol. 141).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cytology
Synaptic Membranes
Molecular biology
Drug Discovery
Synapses
2019

Biotransformation of the mycotoxin enniatin b1 by CYP P450 3A4 and potential for drug-drug interactions

Ivanova, L., Denisov, I. G., Grinkova, Y. V., Sligar, S. G. & Fæste, C. K., Aug 2019, In : Metabolites. 9, 8, 158.

Research output: Contribution to journalArticle

Open Access
Drug interactions
Cytochrome P-450 CYP3A
Mycotoxins
Biotransformation
Drug Interactions
2016

Conformational equilibrium of talin is regulated by anionic lipids

Ye, X., McLean, M. A. & Sligar, S., Aug 1 2016, In : Biochimica et Biophysica Acta - Biomembranes. 1858, 8, p. 1833-1840 8 p.

Research output: Contribution to journalArticle

Talin
Integrins
Lipids
Phospholipids
Membranes
2017

Erratum: Heme Binding Biguanides Target Cytochrome P450-Dependent Cancer Cell Mitochondria (Cell Chemical Biology (2017) 24(10) (1259–1275.e6) (S2451945617302830)(10.1016/j.chembiol.2017.08.009))

Guo, Z., Sevrioukova, I. F., Denisov, I. G., Zhang, X., Chiu, T. L., Thomas, D. G., Hanse, E. A., Cuellar, R. A. D., Grinkova, Y. V., Langenfeld, V. W., Swedien, D. S., Stamschror, J. D., Alvarez, J., Luna, F., Galván, A., Bae, Y. K., Wulfkuhle, J. D., Gallagher, R. I., Petricoin, E. F., Norris, B. & 17 others, Flory, C. M., Schumacher, R. J., O'Sullivan, M. G., Cao, Q., Chu, H., Lipscomb, J. D., Atkins, W. M., Gupta, K., Kelekar, A., Blair, I. A., Capdevila, J. H., Falck, J. R., Sligar, S. G., Poulos, T. L., Georg, G. I., Ambrose, E. & Potter, D. A., Oct 19 2017, In : Cell chemical biology. 24, 10, 1 p.

Research output: Contribution to journalComment/debate

Biguanides
Mitochondria
Heme
Cytochrome P-450 Enzyme System
Cell Biology
2018

Human P450 CYP17A1: Control of Substrate Preference by Asparagine 202

Gregory, M. C., Mak, P. J., Khatri, Y., Kincaid, J. R. & Sligar, S. G., Feb 6 2018, In : Biochemistry. 57, 5, p. 764-771 8 p.

Research output: Contribution to journalArticle

17-alpha-Hydroxyprogesterone
Asparagine
Substrates
Enzymes
Pregnenes
2019

PIP2 Influences the Conformational Dynamics of Membrane-Bound KRAS4b

McLean, M. A., Stephen, A. G. & Sligar, S. G., Aug 20 2019, In : Biochemistry. 58, 33, p. 3537-3545 9 p.

Research output: Contribution to journalArticle

Phosphatidylserines
Membranes
Lipids
Phosphatidylinositols
Phospholipids
2016

The Charge Properties of Phospholipid Nanodiscs

Her, C., Filoti, D. I., McLean, M. A., Sligar, S. G., Alexander Ross, J. B., Steele, H. & Laue, T. M., Sep 6 2016, In : Biophysical journal. 111, 5, p. 989-998 10 p.

Research output: Contribution to journalArticle

Phospholipids
Lipids
Anions
Membranes
Membrane Proteins
2019

Correction to: Allosteric interactions in human cytochrome P450 CYP3A4: The role of phenylalanine 213 (Biochemistry (2019) 58: 10 (1411-1422) DOI: 10.1021/acs.biochem.8b01268)

Denisov, I. G., Grinkova, Y. V., Nandigrami, P., Shekhar, M., Tajkhorshid, E. & Sligar, S. G., Jun 18 2019, In : Biochemistry. 58, 24, 1 p.

Research output: Contribution to journalComment/debate

Cytochrome P-450 CYP3A
Biochemistry
Phenylalanine
Cytochrome P-450 Enzyme System
2017

Microfluidic platform for efficient Nanodisc assembly, membrane protein incorporation, and purification

Wade, J. H., Jones, J. D., Lenov, I. L., Riordan, C. M., Sligar, S. G. & Bailey, R. C., Jan 1 2017, In : Lab on a chip. 17, 17, p. 2951-2959 9 p.

Research output: Contribution to journalArticle

Microfluidics
Purification
Membrane Proteins
Proteins
Membranes

Interaction of KRas4b with anionic membranes: A special role for PIP2

Gregory, M. C., McLean, M. A. & Sligar, S. G., May 27 2017, In : Biochemical and Biophysical Research Communications. 487, 2, p. 351-355 5 p.

Research output: Contribution to journalArticle

Unithiol
Membranes
Lipids
Fluorescence Polarization
Monomeric GTP-Binding Proteins
2019

NMR analysis of free and lipid nanodisc anchored CEACAM1 membrane proximal peptides with Ca 2+ /CaM

Ghazarian, H., Hu, W., Mao, A., Nguyen, T., Vaidehi, N., Sligar, S. & Shively, J. E., Apr 1 2019, In : Biochimica et Biophysica Acta - Biomembranes. 1861, 4, p. 787-797 11 p.

Research output: Contribution to journalArticle

Nuclear magnetic resonance
Membranes
Lipids
Actins
Peptides
2018

Nanodiscs: A Controlled Bilayer Surface for the Study of Membrane Proteins

McLean, M. A., Gregory, M. C. & Sligar, S., May 20 2018, In : Annual Review of Biophysics. 47, p. 107-124 18 p.

Research output: Contribution to journalReview article

Membrane Proteins
Proteins
Membranes
Lipids
Multiprotein Complexes

Cytochrome b5 enhances androgen synthesis by rapidly reducing the CYP17A1 oxy-complex in the lyase step

Duggal, R., Denisov, I. G. & Sligar, S., Jul 2018, In : FEBS Letters. 592, 13, p. 2282-2288 7 p.

Research output: Contribution to journalLetter

Cytochromes b5
Lyases
Cytochrome P-450 Enzyme System
Androgens
NADPH-Ferrihemoprotein Reductase
2017

Alzheimer’s toxic amyloid beta oligomers: Unwelcome visitors to the Na/K ATPase alpha3 docking station

di Chiara, T., DiNunno, N., Clark, J., Bu, R. L., Cline, E. N., Rollins, M. G., Gong, Y., Brody, D. L., Sligar, S. G., Velasco, P. T., Viola, K. L. & Klein, W. L., Mar 2017, In : Yale Journal of Biology and Medicine. 90, 1, p. 45-61 17 p.

Research output: Contribution to journalArticle

Poisons
Oligomers
Amyloid
Adenosine Triphosphatases
Docks
2019

Influence of Transmembrane Helix Mutations on Cytochrome P450-Membrane Interactions and Function

Mustafa, G., Nandekar, P. P., Camp, T. J., Bruce, N. J., Gregory, M. C., Sligar, S. G. & Wade, R. C., Feb 5 2019, In : Biophysical journal. 116, 3, p. 419-432 14 p.

Research output: Contribution to journalArticle

Cytochrome P-450 Enzyme System
Mutation
Membranes
Catalytic Domain
Cytochromes b5
2017

Nanodiscs in Membrane Biochemistry and Biophysics

Denisov, I. G. & Sligar, S. G., Mar 22 2017, In : Chemical reviews. 117, 6, p. 4669-4713 45 p.

Research output: Contribution to journalReview article

Biophysics
Biochemistry
Membrane Proteins
Membranes
Cell signaling
2016

Nanodiscs for structural and functional studies of membrane proteins

Denisov, I. G. & Sligar, S. G., Jun 7 2016, In : Nature Structural and Molecular Biology. 23, 6, p. 481-486 6 p.

Research output: Contribution to journalReview article

Membrane Proteins
Multiprotein Complexes
Technology
Membranes

Phosphatidylinositol 4,5-Bisphosphate Modulates the Affinity of Talin-1 for Phospholipid Bilayers and Activates Its Autoinhibited Form

Ye, X., McLean, M. A. & Sligar, S., Sep 13 2016, In : Biochemistry. 55, 36, p. 5038-5048 11 p.

Research output: Contribution to journalArticle

Talin
Phosphatidylinositols
Phospholipids
Integrins
Lipid bilayers

Trimerization of the HIV Transmembrane Domain in Lipid Bilayers Modulates Broadly Neutralizing Antibody Binding

Reichart, T. M., Baksh, M. M., Rhee, J. K., Fiedler, J. D., Sligar, S. G., Finn, M. G., Zwick, M. B. & Dawson, P. E., Feb 18 2016, In : Angewandte Chemie - International Edition. 55, 8, p. 2688-2692 5 p.

Research output: Contribution to journalArticle

Lipid bilayers
Neutralizing Antibodies
Antibodies
Peptides
Membranes
2018

Drug-Drug Interactions between Atorvastatin and Dronedarone Mediated by Monomeric CYP3A4

Denisov, I. G., Baylon, J. L., Grinkova, Y. V., Tajkhorshid, E. & Sligar, S. G., Feb 6 2018, In : Biochemistry. 57, 5, p. 805-816 12 p.

Research output: Contribution to journalArticle

Drug interactions
Cytochrome P-450 CYP3A
Drug Interactions
Lactones
Pharmaceutical Preparations
2019

Allosteric Interactions in Human Cytochrome P450 CYP3A4: The Role of Phenylalanine 213

Denisov, I. G., Grinkova, Y. V., Nandigrami, P., Shekhar, M., Tajkhorshid, E. & Sligar, S. G., Mar 12 2019, In : Biochemistry. 58, 10, p. 1411-1422 12 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
Phenylalanine
Cytochrome P-450 Enzyme System
Progesterone
Carbamazepine

The hydrodynamic motion of Nanodiscs

Camp, T., McLean, M., Kato, M., Cheruzel, L. & Sligar, S., May 2019, In : Chemistry and Physics of Lipids. 220, p. 28-35 8 p.

Research output: Contribution to journalArticle

Hydrodynamics
Membrane Proteins
Lipid bilayers
Lipid Bilayers
Membranes