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2016
Thioctic Acid
Biosynthetic Pathways
Keto Acids
Enzymes
Bacillus subtilis

PBR322 vectors having tetracycline-dependent replication

Cronan, J. E., Mar 1 2016, In : Plasmid. 84-85, p. 20-26 7 p.

Research output: Contribution to journalArticle

Tetracycline
Plasmids
Lac Repressors
Tetracycline Resistance
Genetic Vectors
2019
Lysogeny
Bacteriophages
Escherichia coli
Temperature
Microbiological Attachment Sites
2015

A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184

Chakravartty, V. & Cronan, J. E., Sep 1 2015, In : Plasmid. 81, p. 21-26 6 p.

Research output: Contribution to journalArticle

Arabinose
Plasmids
Escherichia coli
Spectinomycin
Ampicillin Resistance
2017

The pimeloyl-CoA synthetase BioW defines a new fold for adenylate-forming enzymes

Estrada, P., Manandhar, M., Dong, S. H., Deveryshetty, J., Agarwal, V., Cronan, J. E. & Nair, S. K., Jun 1 2017, In : Nature chemical biology. 13, 6, p. 668-674 7 p.

Research output: Contribution to journalArticle

Amino Acyl-tRNA Synthetases
Catalytic Domain
Coenzyme A Ligases
Enzymes
Coenzyme A
Pimelic Acids
Biotin
Bacillus subtilis
Fatty Acids
Acids
2015

Experimental strategies for functional annotation and metabolism discovery: Targeted screening of solute binding proteins and unbiased panning of metabolomes

Vetting, M. W., Al-Obaidi, N., Zhao, S., San Francisco, B., Kim, J., Wichelecki, D. J., Bouvier, J. T., Solbiati, J. O., Vu, H., Zhang, X., Rodionov, D. A., Love, J. D., Hillerich, B. S., Seidel, R. D., Quinn, R. J., Osterman, A. L., Cronan, J. E., Jacobson, M. P., Gerlt, J. A. & Almo, S. C., Jan 27 2015, In : Biochemistry. 54, 3, p. 909-931 23 p.

Research output: Contribution to journalArticle

Metabolome
Metabolism
Carrier Proteins
Screening
Libraries
2018

Lipoate-binding proteins and specific lipoate-protein ligases in microbial sulfur oxidation reveal an atpyical role for an old cofactor

Cao, X., Koch, T., Steffens, L., Finkensieper, J., Zigann, R., Cronan, J. E. & Dahl, C., Jul 13 2018, In : eLife. 7, e37439.

Research output: Contribution to journalArticle

Sulfur
Carrier Proteins
Oxidation
Genes
Bacillus subtilis
Thioctic Acid
Glycine Decarboxylase Complex H-Protein
Oxidoreductases
Enzymes
Keto Acids
2017

Expression and activity of the BioH esterase of biotin synthesis is independent of genome context

Cao, X., Zhu, L., Hu, Z. & Cronan, J. E., Dec 1 2017, In : Scientific reports. 7, 1, 2141.

Research output: Contribution to journalArticle

Esterases
Operon
Biotin
Genome
Hydrolases
2018

Advances in synthesis of biotin and assembly of lipoic acid

Cronan, J. E., Dec 2018, In : Current Opinion in Chemical Biology. 47, p. 60-66 7 p.

Research output: Contribution to journalReview article

Thioctic Acid
Biotin
Methylation
Metabolism
Escherichia coli
2016

Unsaturated Fatty Acid Synthesis in the Gastric Pathogen Helicobacter pylori Proceeds via a Backtracking Mechanism

Bi, H., Zhu, L., Jia, J., Zeng, L. & Cronan, J. E., Dec 22 2016, In : Cell chemical biology. 23, 12, p. 1480-1489 10 p.

Research output: Contribution to journalArticle

Pathogens
Unsaturated Fatty Acids
Helicobacter pylori
Acyl Carrier Protein
Stomach
2017
Trifluoperazine
Calmodulin
Fatty Acids
Escherichia coli
Lipids
2015

The atypical occurrence of two biotin protein ligases in Francisella novicida is due to distinct roles in virulence and biotin metabolism

Feng, Y., Chin, C. Y., Chakravartty, V., Gao, R., Crispell, E. K., Weiss, D. S. & Cronan, J. E., Jun 9 2015, In : mBio. 6, 3

Research output: Contribution to journalArticle

Francisella
Ligases
Biotin
Virulence
Proteins
Streptomyces coelicolor
Escherichia coli
Ligases
Enzymes
Adenosine Monophosphate

The conserved modular elements of the acyl carrier proteins of lipid synthesis are only partially interchangeable

Zhu, L. & Cronan, J. E., May 29 2015, In : Journal of Biological Chemistry. 290, 22, p. 13791-13799 9 p.

Research output: Contribution to journalArticle

Acyl Carrier Protein
Lactococcus lactis
Escherichia coli
Lipids
Proteins
2016

A Biotin Biosynthesis Gene Restricted to Helicobacter

Bi, H., Zhu, L., Jia, J. & Cronan, J. E., Feb 12 2016, In : Scientific reports. 6, 21162.

Research output: Contribution to journalArticle

Acyl Carrier Protein
Helicobacter
Biotin
Esters
Helicobacter pylori
2018

Novel Xanthomonas campestris long-chain-specific 3-oxoacyl- acyl carrier protein reductase involved in diffusible signal factor synthesis

Hu, Z., Dong, H., Ma, J. C., Yu, Y., Li, K. H., Guo, Q. Q., Zhang, C., Zhang, W. B., Cao, X., Cronan, J. E. & Wang, H., May 1 2018, In : mBio. 9, 3, e00596-18.

Research output: Contribution to journalArticle

3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
Xanthomonas campestris
Fatty Acids
Growth
Oxidoreductases
2019

Enterococcus faecalis encodes an atypical auxiliary acyl carrier protein required for efficient regulation of fatty acid synthesis by exogenous fatty acids

Zhu, L., Zou, Q., Cao, X. & Cronan, J. E., May 1 2019, In : mBio. 10, 3, e00577-19.

Research output: Contribution to journalArticle

Open Access
Acyl Carrier Protein
Enterococcus faecalis
Fatty Acids
Operon
Oleic Acid
Isomerases
Pseudomonas putida
isomerization
isomerases
soil bacteria
2018
Glycine
Keto Acids
Proteins
Oxidoreductases
Bacillus subtilis
2016
Operon
Ligases
Biotin
Staphylococcus aureus
DNA
2015

Biosynthesis of squalene from farnesyl diphosphate in Bacteria: Three steps catalyzed by three enzymes

Pan, J. J., Solbiati, J. O., Ramamoorthy, G., Hillerich, B. S., Seidel, R. D., Cronan, J. E., Almo, S. C. & Poulter, C. D., May 27 2015, In : ACS Central Science. 1, 2, p. 77-82 6 p.

Research output: Contribution to journalArticle

Squalene
Biosynthesis
Bacteria
Enzymes
Genes
2018
8-amino-7-oxononanoate synthase
coenzyme A
biotin
Biotin
Bacillus subtilis
2019

Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing

Srinivas, S., Hu, Z. & Cronan, J. E., May 2019, In : Plasmid. 103, p. 53-62 10 p.

Research output: Contribution to journalArticle

Replication Origin
Plasmids
Escherichia coli
Isopropyl Thiogalactoside
Clustered Regularly Interspaced Short Palindromic Repeats
2016

An Atypical α/β-Hydrolase Fold Revealed in the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl Esterase BioG from Haemophilus influenzae

Shi, J., Cao, X., Chen, Y., Cronan, J. E. & Guo, Z., Dec 6 2016, In : Biochemistry. 55, 48, p. 6705-6717 13 p.

Research output: Contribution to journalArticle

Acyl Carrier Protein
Haemophilus influenzae
Hydrolases
Esterases
Crystal structure
2019

A novel synthesis of trans-unsaturated fatty acids by the Gram-positive commensal bacterium Enterococcus faecalis FA2-2

Kondakova, T., Kumar, S. & Cronan, J. E., Aug 2019, In : Chemistry and Physics of Lipids. 222, p. 23-35 13 p.

Research output: Contribution to journalArticle

Trans Fatty Acids
Enterococcus faecalis
Gram-Positive Bacteria
Unsaturated Fatty Acids
Bacteria