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Author

  • Huimin Zhao
2020

A genetic toolbox for metabolic engineering of Issatchenkia orientalis

Cao, M., Fatma, Z., Song, X., Hsieh, P. H., Tran, V. G., Lyon, W. L., Sayadi, M., Shao, Z., Yoshikuni, Y. & Zhao, H., May 2020, In : Metabolic Engineering. 59, p. 87-97 11 p.

Research output: Contribution to journalArticle

2012

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

Wen, S., Tan, T. & Zhao, H., Dec 5 2012, In : Journal of Biotechnology. 164, 2, p. 248-253 6 p.

Research output: Contribution to journalArticle

2018

Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding

Wang, P., Liang, J., Shi, L. Z., Wang, Y., Zhang, P., Ouyang, M., Preece, D., Peng, Q., Shao, L., Fan, J., Sun, J., Li, S. S., Berns, M. W., Zhao, H. & Wang, Y., Sep 19 2018, In : ACS Photonics. 5, 9, p. 3565-3574 10 p.

Research output: Contribution to journalArticle

2020

A transaldolase-dependent sulfoglycolysis pathway in Bacillus megaterium DSM 1804

Liu, Y., Wei, Y., Zhou, Y., Ang, E. L., Zhao, H. & Zhang, Y., Oct 1 2020, In : Biochemical and Biophysical Research Communications.

Research output: Contribution to journalArticle

2017

Combining Rh-Catalyzed Diazocoupling and Enzymatic Reduction to Efficiently Synthesize Enantioenriched 2-Substituted Succinate Derivatives

Wang, Y., Bartlett, M. J., Denard, C. A., Hartwig, J. F. & Zhao, H., Apr 7 2017, In : ACS Catalysis. 7, 4, p. 2548-2552 5 p.

Research output: Contribution to journalArticle

2016

Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose

Guo, W., Sheng, J., Zhao, H. & Feng, X., Feb 1 2016, In : Microbial cell factories. 15, 1, 24.

Research output: Contribution to journalArticle

2011

Synthetic biology: Putting synthesis into biology

Liang, J., Luo, Y. & Zhao, H., Jan 2011, In : Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 3, 1, p. 7-20 14 p.

Research output: Contribution to journalReview article

2018

Recent developments in the application of P450 based biocatalysts

Wei, Y., Ang, E. L. & Zhao, H., Apr 2018, In : Current Opinion in Chemical Biology. 43, p. 1-7 7 p.

Research output: Contribution to journalReview article

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J. & Zhao, H., Jul 1 2018, In : Nature Biotechnology. 36, 6, p. 505-508 4 p.

Research output: Contribution to journalArticle

2016

Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate

Sun, H., Yeo, W. L., Lim, Y. H., Chew, X., Smith, D. J., Xue, B., Chan, K. P., Robinson, R. C., Robins, E. G., Zhao, H. & Ang, E. L., Jan 1 2016, In : Angewandte Chemie - International Edition. 55, 46, p. 14277-14280 4 p.

Research output: Contribution to journalArticle

2018

Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system

Fine, J. D., Shpigler, H. Y., Ray, A. M., Beach, N. J., Sankey, A. L., Cash-Ahmed, A., Huang, Z. Y., Astrauskaite, I., Chao, R., Zhao, H. & Robinson, G. E., Sep 2018, In : PloS one. 13, 9, e0203444.

Research output: Contribution to journalArticle

2019

An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine

Yin, J., Wei, Y., Liu, D., Hu, Y., Lu, Q., Lui Ang, E., Zhao, H. & Zhang, Y., Oct 25 2019, In : Journal of Biological Chemistry. 294, 43, p. 15662-15671 10 p.

Research output: Contribution to journalArticle

2013

Synthetic Biology

Zhao, H., Jan 1 2013, Elsevier Inc.

Research output: Book/Report/Conference proceedingBook

2017

Twin-primer non-enzymatic DNA assembly: An efficient and accurate multi-part DNA assembly method

Liang, J., Liu, Z., Ang, E. L. & Zhao, H., 2017, Synthetic Biology Conference, SEED 2017: Engineering, Evolution, and Design. AIChE, p. 45-46 2 p. (Synthetic Biology Conference, SEED 2017: Engineering, Evolution, and Design).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2013

Construction and screening of an antigen-derived peptide library displayed on yeast cell surface for CD4+ T cell epitope identification

Wen, F. & Zhao, H., Sep 20 2013, Immunoproteomics: Methods and Protocols. Fulton, K. & Twine, S. (eds.). p. 245-264 20 p. (Methods in Molecular Biology; vol. 1061).

Research output: Chapter in Book/Report/Conference proceedingChapter

2016

Standardization for natural product synthetic biology

Zhao, H. & Medema, M. H., Aug 2016, In : Natural Product Reports. 33, 8, p. 920-924 5 p.

Research output: Contribution to journalReview article

Biocatalysis for Drug Discovery and Development

Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., May 13 2016, Green Biocatalysis. Wiley, p. 421-455 35 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2020

A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens

Wang, F., Wei, Y., Lu, Q., Ang, E. L., Zhao, H. & Zhang, Y., Jul 2020, In : Bioscience Reports. 40, 7, BSR20201642.

Research output: Contribution to journalArticle

Open Access
2019

Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense

Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In : Biochemical Journal. 476, 11, p. 1605-1619 15 p.

Research output: Contribution to journalArticle

2012

A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency

Jeya, M., Kim, T. S., Kumar Tiwari, M., Li, J., Zhao, H. & Lee, J. K., Dec 2012, In : Molecular BioSystems. 8, 12, p. 3103-3106 4 p.

Research output: Contribution to journalArticle

2017

Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring

Freestone, T. S., Ju, K. S., Wang, B. & Zhao, H., Feb 17 2017, In : ACS synthetic biology. 6, 2, p. 217-223 7 p.

Research output: Contribution to journalArticle

2020

Unraveling the iterative type I polyketide synthases hidden in Streptomyces

Wang, B., Guo, F., Huang, C. & Zhao, H., Apr 14 2020, In : Proceedings of the National Academy of Sciences of the United States of America. 117, 15, p. 8449-8454 6 p.

Research output: Contribution to journalArticle

2016

Members of the rusc protein family interact with Sufu and inhibit vertebrate hedgehog signaling

Jin, Z., Schwend, T., Fu, J., Bao, Z., Liang, J., Zhao, H., Mei, W. & Yang, J., Nov 1 2016, In : Development (Cambridge). 143, 21, p. 3944-3955 12 p.

Research output: Contribution to journalArticle

2011

Cloning and characterization of a type III polyketide synthase from Aspergillus niger

Li, J., Luo, Y., Lee, J. K. & Zhao, H., Oct 15 2011, In : Bioorganic and Medicinal Chemistry Letters. 21, 20, p. 6085-6089 5 p.

Research output: Contribution to journalArticle

2012

Strain Improvement via Evolutionary Engineering

Kim, B., Du, J. & Zhao, H., Oct 24 2012, Engineering Complex Phenotypes in Industrial Strains. John Wiley and Sons, p. 111-131 21 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2013

Construction and engineering of large biochemical pathways via DNA assembler

Shao, Z. & Zhao, H., 2013, Synthetic Biology. Polizzi, K. & Kontoravdi, C. (eds.). p. 85-106 22 p. (Methods in Molecular Biology; vol. 1073).

Research output: Chapter in Book/Report/Conference proceedingChapter

2017

A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis

Xiong, X., Zhang, Y., Yan, J., Jain, S., Chee, S., Ren, B. & Zhao, H., Jun 16 2017, In : ACS synthetic biology. 6, 6, p. 1034-1042 9 p.

Research output: Contribution to journalArticle

2014

Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae

Liang, Y., Si, T., Ang, E. L. & Zhao, H., Jan 1 2014, In : Applied and environmental microbiology. 80, 21, p. 6677-6684 8 p.

Research output: Contribution to journalArticle

A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases

Sun, N. & Zhao, H., 2014, Homing Endonucleases: Methods and Protocols. Humana Press Inc., p. 87-96 10 p. (Methods in Molecular Biology; vol. 1123).

Research output: Chapter in Book/Report/Conference proceedingChapter

2011

Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering

Desieno, M., Du, J. & Zhao, H., Mar 9 2011, Protein Engineering Handbook, Volume 1 & Volume 2. Wiley-VCH, Vol. 2. p. 777-796 20 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2016

High-Efficiency Genome Editing of Streptomyces Species by an Engineered CRISPR/Cas System

Wang, Y., Cobb, R. E. & Zhao, H., 2016, Methods in Enzymology. O'Connor, S. E. (ed.). Academic Press Inc., p. 271-284 14 p. (Methods in Enzymology; vol. 575).

Research output: Chapter in Book/Report/Conference proceedingChapter

2014

SunnyTALEN: A second-generation TALEN system for human genome editing

Sun, N., Bao, Z., Xiong, X. & Zhao, H., Apr 2014, In : Biotechnology and bioengineering. 111, 4, p. 683-691 9 p.

Research output: Contribution to journalArticle

2018

RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

HamediRad, M., Lian, J., Li, H. & Zhao, H., Jun 2018, In : Biotechnology and bioengineering. 115, 6, p. 1552-1560 9 p.

Research output: Contribution to journalArticle

2016

High-throughput screening or selection methods for evolutionary enzyme engineering

Shi, S., Zhang, H., Ang, E. L. & Zhao, H., May 17 2016, Understanding Enzymes: Function, Design, Engineering and Analysis. Pan Stanford Publishing Pte. Ltd., p. 707-744 38 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2019

Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

Wen, F., Smith, M. R. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 213-234 22 p. (Methods in Molecular Biology; vol. 2024).

Research output: Chapter in Book/Report/Conference proceedingChapter

2018

Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface

Limsakul, P., Peng, Q., Wu, Y., Allen, M. E., Liang, J., Remacle, A. G., Lopez, T., Ge, X., Kay, B. K., Zhao, H., Strongin, A. Y., Yang, X. L., Lu, S. & Wang, Y., Apr 19 2018, In : Cell chemical biology. 25, 4, p. 370-379.e4

Research output: Contribution to journalArticle

Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction

Singh, R. K., Singh, R., Sivakumar, D., Kondaveeti, S., Kim, T., Li, J., Sung, B. H., Cho, B. K., Kim, D. R., Kim, S. C., Kalia, V. C., Zhang, Y. H. P. J., Zhao, H., Kang, Y. C. & Lee, J. K., Dec 7 2018, In : ACS Catalysis. 8, 12, p. 11085-11093 9 p.

Research output: Contribution to journalArticle

2012

Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration

Zou, Y., Zhang, H., Brunzelle, J. S., Johannes, T. W., Woodyer, R., Hung, J. E., Nair, N., Van Der Donk, W. A., Zhao, H. & Nair, S. K., May 29 2012, In : Biochemistry. 51, 21, p. 4263-4270 8 p.

Research output: Contribution to journalArticle

2020

Recent advances in domesticating non-model microorganisms

Fatma, Z., Schultz, J. C. & Zhao, H., 2020, (Accepted/In press) In : Biotechnology Progress.

Research output: Contribution to journalReview article

Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways

Ren, H., Shi, C. & Zhao, H., Jan 24 2020, In : iScience. 23, 1, 100795.

Research output: Contribution to journalReview article

Open Access
2017
2013

Multistep one-pot reactions combining biocatalysts and chemical catalysts for asymmetric synthesis

Denard, C. A., Hartwig, J. F. & Zhao, H., Dec 6 2013, In : ACS Catalysis. 3, 12, p. 2856-2864 9 p.

Research output: Contribution to journalReview article

2017

A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae

Ren, H., Hu, P. & Zhao, H., Aug 2017, In : Biotechnology and bioengineering. 114, 8, p. 1847-1854 8 p.

Research output: Contribution to journalArticle

2012

Directed evolution: An evolving and enabling synthetic biology tool

Cobb, R. E., Si, T. & Zhao, H., Aug 1 2012, In : Current Opinion in Chemical Biology. 16, 3-4, p. 285-291 7 p.

Research output: Contribution to journalReview article

2020

Discovery and Characterization of a Class IV Lanthipeptide with a Nonoverlapping Ring Pattern

Ren, H., Shi, C., Bothwell, I. R., Van Der Donk, W. A., Van Der Donk, W. A., Van Der Donk, W. A., Zhao, H., Zhao, H., Zhao, H., Zhao, H. & Zhao, H., Jun 19 2020, In : ACS chemical biology. 15, 6, p. 1642-1649 8 p.

Research output: Contribution to journalArticle

2013

Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster

Luo, Y., Huang, H., Liang, J., Wang, M., Lu, L., Shao, Z., Cobb, R. E. & Zhao, H., Dec 5 2013, In : Nature communications. 4, 2894.

Research output: Contribution to journalArticle

2017

Automated multiplex genome-scale engineering in yeast

Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., 2017, In : Nature communications. 8, 15187.

Research output: Contribution to journalArticle

2014

DNA assembly techniques for next-generation combinatorial biosynthesis of natural products

Cobb, R. E., Ning, J. C. & Zhao, H., Feb 2014, In : Journal of Industrial Microbiology and Biotechnology. 41, 2, p. 469-477 9 p.

Research output: Contribution to journalReview article

2013

Coordinated induction of multi-gene pathways in Saccharomyces cerevisiae

Liang, J., Ning, J. C. & Zhao, H., Feb 2013, In : Nucleic acids research. 41, 4, p. e54

Research output: Contribution to journalArticle