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Author

  • Huimin Zhao
2012

DNA assembler: A synthetic biology tool for characterizing and engineering natural product gene clusters

Shao, Z. & Zhao, H., 2012, Natural Product Biosynthesis by Microorganisms and Plants, Part C. Academic Press Inc., p. 203-224 22 p. (Methods in Enzymology; vol. 517).

Research output: Chapter in Book/Report/Conference proceedingChapter

2016

Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae

Wang, M., Li, S. & Zhao, H., Jan 1 2016, In : Biotechnology and bioengineering. 113, 1, p. 206-215 10 p.

Research output: Contribution to journalArticle

2019

Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098

Nguyen, K., DeSieno, M. A., Bae, B., Johannes, T. W., Cobb, R. E., Zhao, H. & Nair, S. K., 2019, In : Organic and Biomolecular Chemistry. 17, 6, p. 1506-1518 13 p.

Research output: Contribution to journalArticle

2016

Use of genome-editing tools to treat sickle cell disease

Tasan, I., Jain, S. & Zhao, H., Sep 1 2016, In : Human Genetics. 135, 9, p. 1011-1028 18 p.

Research output: Contribution to journalReview article

2013

Challenges and opportunities in synthetic biology for chemical engineers

Luo, Y., Lee, J. K. & Zhao, H., Nov 5 2013, In : Chemical Engineering Science. 103, p. 115-119 5 p.

Research output: Contribution to journalArticle

2016
2012

Direct cloning of large genomic sequences

Cobb, R. E. & Zhao, H., May 1 2012, In : Nature Biotechnology. 30, 5, p. 405-406 2 p.

Research output: Contribution to journalShort survey

2020

Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution

Xue, P., Si, T. & Zhao, H., 2020, Methods in Enzymology. Tawfik, D. S. (ed.). Academic Press Inc., p. 255-273 19 p. (Methods in Enzymology; vol. 644).

Research output: Chapter in Book/Report/Conference proceedingChapter

2018

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains

Lian, J., Bao, Z., Hu, S. & Zhao, H., Jun 2018, In : Biotechnology and bioengineering. 115, 6, p. 1630-1635 6 p.

Research output: Contribution to journalArticle

2014

Aryl-aldehyde formation in fungal polyketides: Discovery and characterization of a distinct biosynthetic mechanism

Wang, M., Beissner, M. & Zhao, H., Feb 20 2014, In : Chemistry and Biology. 21, 2, p. 257-263 7 p.

Research output: Contribution to journalArticle

2013

Directed evolution as a powerful synthetic biology tool

Cobb, R. E., Sun, N. & Zhao, H., Mar 15 2013, In : Methods. 60, 1, p. 81-90 10 p.

Research output: Contribution to journalReview article

2011

A new fluorescence complementation biosensor for detection of estrogenic compounds

Mclachlan, M. J., Katzenellenbogen, J. A. & Zhao, H., Dec 2011, In : Biotechnology and bioengineering. 108, 12, p. 2794-2803 10 p.

Research output: Contribution to journalArticle

2014

A single-chain TALEN architecture for genome engineering

Sun, N. & Zhao, H., Mar 1 2014, In : Molecular BioSystems. 10, 3, p. 446-453 8 p.

Research output: Contribution to journalArticle

2020

Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces

Ye, S., Enghiad, B., Zhao, H. & Takano, E., May 1 2020, In : Journal of Industrial Microbiology and Biotechnology. 47, 4-5, p. 413-423 11 p.

Research output: Contribution to journalArticle

Open Access
2016

Combinatorial pathway engineering for optimized production of the anti-malarial FR900098

Freestone, T. S. & Zhao, H., Feb 1 2016, In : Biotechnology and bioengineering. 113, 2, p. 384-392 9 p.

Research output: Contribution to journalArticle

2014

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

Lian, J., Si, T., Nair, N. U. & Zhao, H., Jul 2014, In : Metabolic Engineering. 24, p. 139-149 11 p.

Research output: Contribution to journalArticle

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

Lian, J., Si, T., Nair, N. U. & Zhao, H., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, p. 750-760 11 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2011

Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD)

Tiwari, M. K., Lee, J. K., Moon, H. J. & Zhao, H., May 15 2011, In : Bioorganic and Medicinal Chemistry Letters. 21, 10, p. 2873-2876 4 p.

Research output: Contribution to journalArticle

2019

An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In : Nature chemical biology. 16, 4, p. 387-390 4 p.

Research output: Contribution to journalArticle

2013

Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial saccharomyces cerevisiae strain

Yuan, Y. & Zhao, H., Nov 2013, In : Biotechnology and bioengineering. 110, 11, p. 2874-2881 8 p.

Research output: Contribution to journalArticle

2011

Drug Discovery and Development by Combinatorial Biosynthesis

Desieno, M. A., Denard, C. A. & Zhao, H., May 25 2011, Enzyme Technologies: Metagenomics, Evolution, Biocatalysis, and Biosynthesis. John Wiley and Sons, p. 251-283 33 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

New N-acetyltransferase fold in the structure and mechanism of the phosphonate biosynthetic enzyme FrbF

Bae, B., Cobb, R. E., DeSieno, M. A., Zhao, H. & Nair, S. K., Oct 14 2011, In : Journal of Biological Chemistry. 286, 41, p. 36132-36141 10 p.

Research output: Contribution to journalArticle

2017

Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

Abil, Z., Gumy, L. F., Zhao, H. & Hoogenraad, C. C., Jun 16 2017, In : ACS synthetic biology. 6, 6, p. 950-956 7 p.

Research output: Contribution to journalArticle

2013
2014

Homology-integrated CRISPR-cas (HI-CRISPR) system for one-step multigene disruption in saccharomyces cerevisiae

Bao, Z., Xiao, H., Liang, J., Zhang, L., Xiong, X., Sun, N., Si, T. & Zhao, H., Jan 1 2014, Systems Biology 2014 - Topical Conference at the 2014 AIChE Annual Meeting. AIChE, p. 3-12 10 p. (Systems Biology 2014 - Topical Conference at the 2014 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2011

Cell surface display of functional human MHC class II proteins: Yeast display versus insect cell display

Wen, F., Sethi, D. K., Wucherpfennig, K. W. & Zhao, H., Sep 1 2011, In : Protein Engineering, Design and Selection. 24, 9, p. 701-709 9 p.

Research output: Contribution to journalArticle

2013

Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands

Liang, J., Mclachlan, M. J. & Zhao, H., May 1 2013, In : Biotechnology and bioengineering. 110, 5, p. 1419-1429 11 p.

Research output: Contribution to journalArticle

Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analysis

Feng, X. & Zhao, H., Nov 18 2013, In : Microbial cell factories. 12, 1, 114.

Research output: Contribution to journalArticle

2016

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

Yuan, Y., Andersen, E. & Zhao, H., Jan 15 2016, In : ACS synthetic biology. 5, 1, p. 46-52 7 p.

Research output: Contribution to journalArticle

2018

Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli

Si, T., Tian, Q., Min, Y., Zhang, L., Sweedler, J. V., Van Der Donk, W. A. & Zhao, H., Sep 26 2018, In : Journal of the American Chemical Society. 140, 38, p. 11884-11888 5 p.

Research output: Contribution to journalArticle

CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci

Tasan, I., Sustackova, G., Zhang, L., Kim, J., Sivaguru, M., HamediRad, M., Wang, Y., Genova, J., Ma, J., Belmont, A. S. & Zhao, H., 2018, In : Nucleic acids research. 46, 17, e100.

Research output: Contribution to journalArticle

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In : Biotechnology Journal. 13, 9, 1700601.

Research output: Contribution to journalReview article

2012

DNA assembler method for construction of zeaxanthin-producing strains of saccharomyces cerevisiae

Shao, Z., Luo, Y. & Zhao, H., 2012, Microbial Carotenoids From Fungi: Methods and Protocols. Jose-Luis, B. (ed.). p. 251-262 12 p. (Methods in Molecular Biology; vol. 898).

Research output: Chapter in Book/Report/Conference proceedingChapter

2013

Customized optimization of metabolic pathways by combinatorial transcriptional engineering.

Yuan, Y., Du, J. & Zhao, H., Aug 2 2013, In : Methods in molecular biology (Clifton, N.J.). 985, p. 177-209 33 p.

Research output: Contribution to journalArticle

2016

Tandem reactions combining biocatalysts and chemical catalysts for asymmetric synthesis

Wang, Y. & Zhao, H., Dec 1 2016, In : Catalysts. 6, 12, 194.

Research output: Contribution to journalReview article

2017

Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators

Bao, Z., Jain, S., Jaroenpuntaruk, V. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 686-693 8 p.

Research output: Contribution to journalArticle

2013

Combinatorial design of a highly efficient xylose-utilizing pathway in Saccharomyces cerevisiae for the production of cellulosic biofuels

Kim, B., Du, J., Eriksen, D. T. & Zhao, H., Feb 2013, In : Applied and environmental microbiology. 79, 3, p. 931-941 11 p.

Research output: Contribution to journalArticle

Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production

Rao, G., Lee, J. K. & Zhao, H., Jul 1 2013, In : Applied Microbiology and Biotechnology. 97, 13, p. 5861-5867 7 p.

Research output: Contribution to journalArticle

2017

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Lian, J., Hamedirad, M., Hu, S. & Zhao, H., Dec 1 2017, In : Nature communications. 8, 1, 1688.

Research output: Contribution to journalArticle

2018
2014

Recent advances in natural product discovery

Luo, Y., Cobb, R. E. & Zhao, H., Dec 1 2014, In : Current Opinion in Biotechnology. 30, p. 230-237 8 p.

Research output: Contribution to journalReview article

2018

In vivo biosensors: mechanisms, development, and applications

Shi, S., Ang, E. L. & Zhao, H., Jul 1 2018, In : Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 491-516 26 p.

Research output: Contribution to journalArticle

2020

Integrating biocatalysis with chemocatalysis for selective transformations

Huang, X., Cao, M. & Zhao, H., Apr 2020, In : Current Opinion in Chemical Biology. 55, p. 161-170 10 p.

Research output: Contribution to journalReview article

2019

Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

Lian, J., Schultz, C., Cao, M., HamediRad, M. & Zhao, H., Dec 1 2019, In : Nature communications. 10, 1, 5794.

Research output: Contribution to journalArticle

Open Access
2014

Cooperative tandem catalysis by an organometallic complex and a metalloenzyme

Denard, C. A., Huang, H., Bartlett, M. J., Lu, L., Tan, Y., Zhao, H. & Hartwig, J. F., Jan 7 2014, In : Angewandte Chemie - International Edition. 53, 2, p. 465-469 5 p.

Research output: Contribution to journalArticle

2018

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In : Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.

Research output: Contribution to journalReview article

2017

Using natural products for drug discovery: the impact of the genomics era

Zhang, M. M., Qiao, Y., Ang, E. L. & Zhao, H., May 4 2017, In : Expert Opinion on Drug Discovery. 12, 5, p. 475-487 13 p.

Research output: Contribution to journalReview article

2011

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

Shao, Z., Luo, Y. & Zhao, H., Apr 1 2011, In : Molecular BioSystems. 7, 4, p. 1056-1059 4 p.

Research output: Contribution to journalArticle

2017

Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry

Chao, R., Liang, J., Tasan, I., Si, T., Ju, L. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 678-685 8 p.

Research output: Contribution to journalArticle