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  • Huimin Zhao
2013

Construction and screening of an antigen-derived peptide library displayed on yeast cell surface for CD4+ T cell epitope identification

Wen, F. & Zhao, H., Sep 20 2013, Immunoproteomics: Methods and Protocols. Fulton, K. & Twine, S. (eds.). p. 245-264 20 p. (Methods in Molecular Biology; vol. 1061).

Research output: Chapter in Book/Report/Conference proceedingChapter

2015

Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening

Li, S., Si, T., Wang, M. & Zhao, H., Dec 18 2015, In : ACS synthetic biology. 4, 12, p. 1308-1315 8 p.

Research output: Contribution to journalArticle

2012

Investigation of the role of Arg301 identified in the X-ray structure of phosphite dehydrogenase

Hung, J. E., Fogle, E. J., Christman, H. D., Johannes, T. W., Zhao, H., Metcalf, W. W. & Van Der Donk, W. A., May 29 2012, In : Biochemistry. 51, 21, p. 4254-4262 9 p.

Research output: Contribution to journalArticle

A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency

Jeya, M., Kim, T. S., Kumar Tiwari, M., Li, J., Zhao, H. & Lee, J. K., Dec 2012, In : Molecular BioSystems. 8, 12, p. 3103-3106 4 p.

Research output: Contribution to journalArticle

2018

CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci

Tasan, I., Sustackova, G., Zhang, L., Kim, J., Sivaguru, M., HamediRad, M., Wang, Y., Genova, J., Ma, J., Belmont, A. S. & Zhao, H., 2018, In : Nucleic acids research. 46, 17, e100.

Research output: Contribution to journalArticle

2013

Construction and engineering of large biochemical pathways via DNA assembler

Shao, Z. & Zhao, H., 2013, Synthetic Biology. Polizzi, K. & Kontoravdi, C. (eds.). p. 85-106 22 p. (Methods in Molecular Biology; vol. 1073).

Research output: Chapter in Book/Report/Conference proceedingChapter

2016

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

Yuan, Y., Andersen, E. & Zhao, H., Jan 15 2016, In : ACS synthetic biology. 5, 1, p. 46-52 7 p.

Research output: Contribution to journalArticle

2015

Engineered biosynthesis of natural products in heterologous hosts

Luo, Y., Li, B. Z., Liu, D., Zhang, L., Chen, Y., Jia, B., Zeng, B. X., Zhao, H. & Yuan, Y. J., Aug 7 2015, In : Chemical Society Reviews. 44, 15, p. 5265-5290 26 p.

Research output: Contribution to journalArticle

2018

Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A. N., Liu, Y., Ang, E. L., Zhao, S., Zhao, H. & Zhang, Y., Dec 1 2018, In : Nature communications. 9, 1, 4224.

Research output: Contribution to journalArticle

2020

Evolutionary context-integrated deep sequence modeling for protein engineering

Luo, Y., Vo, L., Ding, H., Su, Y., Liu, Y., Qian, W. W., Zhao, H. & Peng, J., 2020, Research in Computational Molecular Biology - 24th Annual International Conference, RECOMB 2020, Proceedings. Schwartz, R. (ed.). Springer, p. 261-263 3 p. (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); vol. 12074 LNBI).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2018

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J. & Zhao, H., Jul 1 2018, In : Nature Biotechnology. 36, 6, p. 505-508 4 p.

Research output: Contribution to journalArticle

2019

Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense

Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In : Biochemical Journal. 476, 11, p. 1605-1619 15 p.

Research output: Contribution to journalArticle

2018

A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

Sun, H., Zhao, H. & Ang, E. L., 2018, In : Chemical Communications. 54, 68, p. 9458-9461 4 p.

Research output: Contribution to journalArticle

2016

Combinatorial pathway engineering for optimized production of the anti-malarial FR900098

Freestone, T. S. & Zhao, H., Feb 1 2016, In : Biotechnology and bioengineering. 113, 2, p. 384-392 9 p.

Research output: Contribution to journalArticle

2014

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

Lian, J., Si, T., Nair, N. U. & Zhao, H., Jul 2014, In : Metabolic Engineering. 24, p. 139-149 11 p.

Research output: Contribution to journalArticle

2011

Drug Discovery and Development by Combinatorial Biosynthesis

Desieno, M. A., Denard, C. A. & Zhao, H., May 25 2011, Enzyme Technologies: Metagenomics, Evolution, Biocatalysis, and Biosynthesis. John Wiley and Sons, p. 251-283 33 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo

Evans, B. S., Chen, Y., Metcalf, W. W., Zhao, H. & Kelleher, N. L., May 27 2011, In : Chemistry and Biology. 18, 5, p. 601-607 7 p.

Research output: Contribution to journalArticle

2012

Mutagenic inverted repeats assisted genome engineering (MIRAGE) in saccharomyces cerevisiae: Deletion of gal7

Nair, N. U. & Zhao, H., Jan 20 2012, Microbial Metabolic Engineering: Methods and Protocols. Cheng, Q. (ed.). p. 63-73 11 p. (Methods in Molecular Biology; vol. 834).

Research output: Chapter in Book/Report/Conference proceedingChapter

2015

Reversal of the β-oxidation cycle in saccharomyces cerevisiae for production of fuels and chemicals

Lian, J. & Zhao, H., Mar 20 2015, In : ACS synthetic biology. 4, 3, p. 332-341 10 p.

Research output: Contribution to journalArticle

Building biological foundries for next-generation synthetic biology

Chao, R., Yuan, Y. B. & Zhao, H. M., Jul 24 2015, In : Science China Life Sciences. 58, 7, p. 658-665 8 p.

Research output: Contribution to journalReview article

2016

Biocatalysis for Drug Discovery and Development

Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., May 13 2016, Green Biocatalysis. Wiley, p. 421-455 35 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2018

RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

HamediRad, M., Lian, J., Li, H. & Zhao, H., Jun 2018, In : Biotechnology and bioengineering. 115, 6, p. 1552-1560 9 p.

Research output: Contribution to journalArticle

2014

DNA assembly techniques for next-generation combinatorial biosynthesis of natural products

Cobb, R. E., Ning, J. C. & Zhao, H., Feb 2014, In : Journal of Industrial Microbiology and Biotechnology. 41, 2, p. 469-477 9 p.

Research output: Contribution to journalReview article

2012

Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease

Sun, N., Liang, J., Abil, Z. & Zhao, H., Mar 1 2012, In : Molecular BioSystems. 8, 4, p. 1255-1263 9 p.

Research output: Contribution to journalArticle

2016

Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae

Wang, M., Li, S. & Zhao, H., Jan 1 2016, In : Biotechnology and bioengineering. 113, 1, p. 206-215 10 p.

Research output: Contribution to journalArticle

2017

Strain development by whole-cell directed evolution

Si, T., Lian, J. & Zhao, H., Jan 1 2017, Directed Enzyme Evolution: Advances and Applications. Springer International Publishing, p. 173-200 28 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators

Bao, Z., Jain, S., Jaroenpuntaruk, V. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 686-693 8 p.

Research output: Contribution to journalArticle

2012

DNA assembler method for construction of zeaxanthin-producing strains of saccharomyces cerevisiae

Shao, Z., Luo, Y. & Zhao, H., 2012, Microbial Carotenoids From Fungi: Methods and Protocols. Jose-Luis, B. (ed.). p. 251-262 12 p. (Methods in Molecular Biology; vol. 898).

Research output: Chapter in Book/Report/Conference proceedingChapter

2017

Programmable DNA-Guided Artificial Restriction Enzymes

Enghiad, B. & Zhao, H., May 19 2017, In : ACS synthetic biology. 6, 5, p. 752-757 6 p.

Research output: Contribution to journalArticle

2018

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In : Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.

Research output: Contribution to journalReview article

2016

TALE proteins search DNA using a rotationally decoupled mechanism

Cuculis, L., Abil, Z., Zhao, H. & Schroeder, C. M., Oct 1 2016, In : Nature chemical biology. 12, 10, p. 831-837 7 p.

Research output: Contribution to journalArticle

2014

Exploiting Issatchenkia orientalis SD108 for succinic acid production

Xiao, H., Shao, Z., Jiang, Y., Dole, S. & Zhao, H., Aug 27 2014, In : Microbial cell factories. 13, 1, 121.

Research output: Contribution to journalReview article

2011

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

Shao, Z., Luo, Y. & Zhao, H., Apr 1 2011, In : Molecular BioSystems. 7, 4, p. 1056-1059 4 p.

Research output: Contribution to journalArticle

2020

Divalent cations promote TALE DNA-binding specificity

Cuculis, L., Zhao, C., Abil, Z., Zhao, H., Shukla, D. & Schroeder, C. M., Feb 20 2020, In : Nucleic acids research. 48, 3, p. 1406-1422 17 p.

Research output: Contribution to journalArticle

Open Access
2011

Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering

Desieno, M., Du, J. & Zhao, H., Mar 9 2011, Protein Engineering Handbook, Volume 1 & Volume 2. Wiley-VCH, Vol. 2. p. 777-796 20 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2017

Metabolic engineering of oleaginous yeasts for production of fuels and chemicals

Shi, S. & Zhao, H., Nov 8 2017, In : Frontiers in Microbiology. 8, NOV, 2185.

Research output: Contribution to journalReview article

2018

Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications

Lian, J., Mishra, S. & Zhao, H., Nov 2018, In : Metabolic Engineering. 50, p. 85-108 24 p.

Research output: Contribution to journalReview article

2015

DNA-Based Storage: Trends and Methods

Yazdi, S. M. H. T., Kiah, H. M., Garcia-Ruiz, E., Ma, J., Zhao, H. & Milenkovic, O., Sep 2015, In : IEEE Transactions on Molecular, Biological, and Multi-Scale Communications. 1, 3, p. 230-248 19 p., 7423733.

Research output: Contribution to journalArticle

2017

Using natural products for drug discovery: the impact of the genomics era

Zhang, M. M., Qiao, Y., Ang, E. L. & Zhao, H., May 4 2017, In : Expert Opinion on Drug Discovery. 12, 5, p. 475-487 13 p.

Research output: Contribution to journalReview article

2020

A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens

Wang, F., Wei, Y., Lu, Q., Ang, E. L., Zhao, H. & Zhang, Y., Jul 2020, In : Bioscience Reports. 40, 7, BSR20201642.

Research output: Contribution to journalArticle

Open Access
2012

Recent advances in targeted genome engineering in mammalian systems

Sun, N., Abil, Z. & Zhao, H., Sep 2012, In : Biotechnology Journal. 7, 9, p. 1074-1087 14 p.

Research output: Contribution to journalReview article

2017

Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry

Chao, R., Liang, J., Tasan, I., Si, T., Ju, L. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 678-685 8 p.

Research output: Contribution to journalArticle

2015

Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus

Luo, Y., Zhang, L., Barton, K. W. & Zhao, H., Apr 29 2015, In : ACS synthetic biology. 4, 9, p. 1001-1010 10 p.

Research output: Contribution to journalArticle

2019

A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In : Metabolic Engineering Communications. 9, e00101.

Research output: Contribution to journalArticle

Open Access
2015

Editorial overview: Opportunities and challenges in synthetic biology

Chang, M. C. Y. & Zhao, H., Oct 1 2015, In : Current Opinion in Chemical Biology. 28, p. 5-6 2 p.

Research output: Contribution to journalEditorial

Selective elimination of mitochondrial mutations in the germline by genome editing

Reddy, P., Ocampo, A., Suzuki, K., Luo, J., Bacman, S. R., Williams, S. L., Sugawara, A., Okamura, D., Tsunekawa, Y., Wu, J., Lam, D., Xiong, X., Montserrat, N., Esteban, C. R., Liu, G. H., Sancho-Martinez, I., Manau, D., Civico, S., Cardellach, F., Del Mar O'Callaghan, M. & 5 others, Campistol, J., Zhao, H., Campistol, J. M., Moraes, C. T. & Izpisua Belmonte, J. C., Apr 23 2015, In : Cell. 161, 3, p. 459-469 11 p.

Research output: Contribution to journalArticle

2018

Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding

Wang, P., Liang, J., Shi, L. Z., Wang, Y., Zhang, P., Ouyang, M., Preece, D., Peng, Q., Shao, L., Fan, J., Sun, J., Li, S. S., Berns, M. W., Zhao, H. & Wang, Y., Sep 19 2018, In : ACS Photonics. 5, 9, p. 3565-3574 10 p.

Research output: Contribution to journalArticle

2020

A genetic toolbox for metabolic engineering of Issatchenkia orientalis

Cao, M., Fatma, Z., Song, X., Hsieh, P. H., Tran, V. G., Lyon, W. L., Sayadi, M., Shao, Z., Yoshikuni, Y. & Zhao, H., May 2020, In : Metabolic Engineering. 59, p. 87-97 11 p.

Research output: Contribution to journalArticle