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  • Huimin Zhao
2013

Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold

Shao, Z., Rao, G., Li, C., Abil, Z., Luo, Y. & Zhao, H., Nov 15 2013, In : ACS synthetic biology. 2, 11, p. 662-669 8 p.

Research output: Contribution to journalArticle

2019

An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In : Nature chemical biology. 16, 4, p. 387-390 4 p.

Research output: Contribution to journalArticle

2012

CmlI is an N-oxygenase in the biosynthesis of chloramphenicol

Lu, H., Chanco, E. & Zhao, H., Sep 16 2012, In : Tetrahedron. 68, 37, p. 7651-7654 4 p.

Research output: Contribution to journalArticle

2020

DNA punch cards for storing data on native DNA sequences via enzymatic nicking

Tabatabaei, S. K., Wang, B., Athreya, N. B. M., Enghiad, B., Hernandez, A. G., Fields, C. J., Leburton, J. P., Soloveichik, D., Zhao, H. & Milenkovic, O., Dec 1 2020, In : Nature communications. 11, 1, 1742.

Research output: Contribution to journalArticle

Open Access
2015

Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic

Badur, A. H., Jagtap, S. S., Yalamanchili, G., Lee, J. K., Zhao, H. & Rao, C. V., Jan 1 2015, In : Applied and environmental microbiology. 81, 5, p. 1865-1873 9 p.

Research output: Contribution to journalArticle

2018

Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction

Singh, R. K., Singh, R., Sivakumar, D., Kondaveeti, S., Kim, T., Li, J., Sung, B. H., Cho, B. K., Kim, D. R., Kim, S. C., Kalia, V. C., Zhang, Y. H. P. J., Zhao, H., Kang, Y. C. & Lee, J. K., Dec 7 2018, In : ACS Catalysis. 8, 12, p. 11085-11093 9 p.

Research output: Contribution to journalArticle

2020

Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways

Ren, H., Shi, C. & Zhao, H., Jan 24 2020, In : iScience. 23, 1, 100795.

Research output: Contribution to journalReview article

Open Access
2016

Identification of an important motif that controls the activity and specificity of sugar transporters

Wang, M., Yu, C. & Zhao, H., Jul 1 2016, In : Biotechnology and bioengineering. 113, 7, p. 1460-1467 8 p.

Research output: Contribution to journalArticle

2015

Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production

Feng, X., Lian, J. & Zhao, H., Jan 1 2015, In : Metabolic Engineering. 27, p. 10-19 10 p.

Research output: Contribution to journalArticle

2017

Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring

Freestone, T. S., Ju, K. S., Wang, B. & Zhao, H., Feb 17 2017, In : ACS synthetic biology. 6, 2, p. 217-223 7 p.

Research output: Contribution to journalArticle

2013

Multistep one-pot reactions combining biocatalysts and chemical catalysts for asymmetric synthesis

Denard, C. A., Hartwig, J. F. & Zhao, H., Dec 6 2013, In : ACS Catalysis. 3, 12, p. 2856-2864 9 p.

Research output: Contribution to journalReview article

2019

Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria

Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., N. Nanjaraj Urs, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Dec 1 2019, In : Nature communications. 10, 1, 1609.

Research output: Contribution to journalArticle

Open Access
2016

Combined and iterative use of computational design and directed evolution for protein-ligand binding design

Wang, M. & Zhao, H., May 1 2016, Methods in Molecular Biology. Humana Press Inc., p. 139-153 15 p. (Methods in Molecular Biology; vol. 1414).

Research output: Chapter in Book/Report/Conference proceedingChapter

2019

Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense

Zhou, Y., Wei, Y., Nanjaraj Urs, A. N., Lin, L., Xu, T., Hu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., 2019, In : Bioscience Reports. 39, 6, Y.

Research output: Contribution to journalArticle

Open Access
2013

Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

Li, S., Ha, S. J., Kim, H. J., Galazka, J. M., Cate, J. H. D., Jin, Y. S. & Zhao, H., Oct 10 2013, In : Journal of Biotechnology. 168, 1, p. 1-6 6 p.

Research output: Contribution to journalArticle

2019

Activation of silent biosynthetic gene clusters using transcription factor decoys

Wang, B., Guo, F., Dong, S. H. & Zhao, H., Feb 1 2019, In : Nature chemical biology. 15, 2, p. 111-114 4 p.

Research output: Contribution to journalArticle

2011

Cell surface display of functional human MHC class II proteins: Yeast display versus insect cell display

Wen, F., Sethi, D. K., Wucherpfennig, K. W. & Zhao, H., Sep 1 2011, In : Protein Engineering, Design and Selection. 24, 9, p. 701-709 9 p.

Research output: Contribution to journalArticle

2015

Recent advances in combinatorial biosynthesis for drug discovery

Sun, H., Liu, Z., Zhao, H. & Ang, E. L., Feb 12 2015, In : Drug Design, Development and Therapy. 9, p. 823-833 11 p.

Research output: Contribution to journalReview article

2017

Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae

Shi, S., Choi, Y. W., Zhao, H., Tan, M. H. & Ang, E. L., Dec 2017, In : Bioresource Technology. 245, p. 1343-1351 9 p.

Research output: Contribution to journalArticle

2012

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

Wen, S., Tan, T. & Zhao, H., Dec 5 2012, In : Journal of Biotechnology. 164, 2, p. 248-253 6 p.

Research output: Contribution to journalArticle

2013

Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands

Liang, J., Mclachlan, M. J. & Zhao, H., May 1 2013, In : Biotechnology and bioengineering. 110, 5, p. 1419-1429 11 p.

Research output: Contribution to journalArticle

Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial saccharomyces cerevisiae strain

Yuan, Y. & Zhao, H., Nov 2013, In : Biotechnology and bioengineering. 110, 11, p. 2874-2881 8 p.

Research output: Contribution to journalArticle

2011

Engineering saccharomyces cerevisiae for biofuel production from lignocellulosic biomass

Du, J. & Zhao, H., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2016

Engineering microbial hosts for production of bacterial natural products

Zhang, M. M., Wang, Y., Ang, E. L. & Zhao, H., Aug 2016, In : Natural Product Reports. 33, 8, p. 963-987 25 p.

Research output: Contribution to journalReview article

2011

Highlights of biocatalysis and biomimetic catalysis

Zhao, H., Sep 2 2011, In : ACS Catalysis. 1, 9, p. 1119-1120 2 p.

Research output: Contribution to journalEditorial

2016

Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate

Sun, H., Yeo, W. L., Lim, Y. H., Chew, X., Smith, D. J., Xue, B., Chan, K. P., Robinson, R. C., Robins, E. G., Zhao, H. & Ang, E. L., Jan 1 2016, In : Angewandte Chemie - International Edition. 55, 46, p. 14277-14280 4 p.

Research output: Contribution to journalArticle

2020

A mass spectrometry-based high-throughput screening method for engineering fatty acid synthases with improved production of medium-chain fatty acids

Xue, P., Si, T., Mishra, S., Zhang, L., Choe, K., Sweedler, J. V. & Zhao, H., Jul 1 2020, In : Biotechnology and bioengineering. 117, 7, p. 2131-2138 8 p.

Research output: Contribution to journalArticle

2017

A New Era of Genome Integration - Simply Cut and Paste!

Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 601-609 9 p.

Research output: Contribution to journalReview article

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Lian, J., Hamedirad, M., Hu, S. & Zhao, H., Dec 1 2017, In : Nature communications. 8, 1, 1688.

Research output: Contribution to journalArticle

2014

Characterization of the N-oxygenase AurF from Streptomyces thioletus

Chanco, E., Choi, Y. S., Sun, N., Vu, M. & Zhao, H., Oct 15 2014, In : Bioorganic and Medicinal Chemistry. 22, 20, p. 5569-5577 9 p.

Research output: Contribution to journalArticle

2012

Strain Improvement via Evolutionary Engineering

Kim, B., Du, J. & Zhao, H., Oct 24 2012, Engineering Complex Phenotypes in Industrial Strains. John Wiley and Sons, p. 111-131 21 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2016

Characterization of bacillus subtilis colony biofilms via mass spectrometry and fluorescence imaging

Si, T., Li, B., Zhang, K., Xu, Y., Zhao, H. & Sweedler, J. V., Jun 3 2016, In : Journal of Proteome Research. 15, 6, p. 1955-1962 8 p.

Research output: Contribution to journalArticle

2014

Cooperative tandem catalysis by an organometallic complex and a metalloenzyme

Denard, C. A., Huang, H., Bartlett, M. J., Lu, L., Tan, Y., Zhao, H. & Hartwig, J. F., Jan 7 2014, In : Angewandte Chemie - International Edition. 53, 2, p. 465-469 5 p.

Research output: Contribution to journalArticle

2016

A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

Shi, S., Liang, Y., Zhang, M. M., Ang, E. L. & Zhao, H., Jan 1 2016, In : Metabolic Engineering. 33, p. 19-27 9 p.

Research output: Contribution to journalArticle

2014

Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in d-tagatose production

Jagtap, S. S., Singh, R., Kang, Y. C., Zhao, H. & Lee, J. K., May 10 2014, In : Enzyme and Microbial Technology. 58-59, p. 44-51 8 p.

Research output: Contribution to journalArticle

2013

Pathway engineering as an enabling synthetic biology tool

Eriksen, D. T., Li, S. & Zhao, H., Aug 27 2013, Synthetic Biology. Elsevier Inc., p. 43-61 19 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2016

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Luo, Y., Enghiad, B. & Zhao, H., Feb 2016, In : Natural Product Reports. 33, 2, p. 174-182 9 p.

Research output: Contribution to journalArticle

2015

Direct observation of TALE protein dynamics reveals a two-state search mechanism

Cuculis, L., Abil, Z., Zhao, H. & Schroeder, C. M., Jun 1 2015, In : Nature communications. 6, 7277.

Research output: Contribution to journalArticle

2017

Twin-primer non-enzymatic DNA assembly: An efficient and accurate multi-part DNA assembly method

Liang, J., Liu, Z., Low, X. Z., Ang, E. L. & Zhao, H., Jun 1 2017, In : Nucleic acids research. 45, 11, p. e94

Research output: Contribution to journalArticle

2012

Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome

Sun, J., Wen, F., Si, T., Xu, J. H. & Zhao, H., Jun 1 2012, In : Applied and environmental microbiology. 78, 11, p. 3837-3845 9 p.

Research output: Contribution to journalArticle

2019

Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098

Nguyen, K., DeSieno, M. A., Bae, B., Johannes, T. W., Cobb, R. E., Zhao, H. & Nair, S. K., 2019, In : Organic and Biomolecular Chemistry. 17, 6, p. 1506-1518 13 p.

Research output: Contribution to journalArticle

2020

Photoenzymatic enantioselective intermolecular radical hydroalkylation

Huang, X., Wang, B., Wang, Y., Jiang, G., Feng, J. & Zhao, H., Aug 6 2020, In : Nature. 584, 7819, p. 69-74 6 p.

Research output: Contribution to journalArticle

2013

Combinatorial design of a highly efficient xylose-utilizing pathway in Saccharomyces cerevisiae for the production of cellulosic biofuels

Kim, B., Du, J., Eriksen, D. T. & Zhao, H., Feb 2013, In : Applied and environmental microbiology. 79, 3, p. 931-941 11 p.

Research output: Contribution to journalArticle

2020

Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces

Ye, S., Enghiad, B., Zhao, H. & Takano, E., May 1 2020, In : Journal of Industrial Microbiology and Biotechnology. 47, 4-5, p. 413-423 11 p.

Research output: Contribution to journalArticle

Open Access
2018

Applying advanced dna assembly methods to generate pathway libraries

Eriksen, D. T., Chao, R. & Zhao, H., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 331-347 17 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2020

Biosystems design by directed evolution

Wang, Y., Yu, X. & Zhao, H., Mar 1 2020, In : AIChE Journal. 66, 3, e16716.

Research output: Contribution to journalReview article

2014
2019

Delta integration CRISPR-Cas (Di-CRISPR) in saccharomyces cerevisiae

Shi, S., Liang, Y., Ang, E. L. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 73-91 19 p. (Methods in Molecular Biology; vol. 1927).

Research output: Chapter in Book/Report/Conference proceedingChapter