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2010

A diverse family of type III polyketide synthases in Eucalyptus species

Rubin-Pitel, S. B., Luo, Y., Lee, J. K. & Zhao, H., Aug 1 2010, In : Molecular BioSystems. 6, 8, p. 1444-1446 3 p.

Research output: Contribution to journalArticle

Eucalyptus
Polyketides
Enzymes
Biological Products
Genes
2019

Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides

Schultz, J. C., Cao, M. & Zhao, H., Aug 2019, In : Biotechnology and bioengineering. 116, 8, p. 2103-2109 7 p.

Research output: Contribution to journalArticle

Clustered Regularly Interspaced Short Palindromic Repeats
Gene Knockout Techniques
Gene Deletion
Guide RNA
Genes

Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca

Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In : Biochemical Journal. 476, 4, p. 733-746 14 p.

Research output: Contribution to journalArticle

Klebsiella oxytoca
Oxidoreductases
Taurine
NADP
Tail
2013

Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial saccharomyces cerevisiae strain

Yuan, Y. & Zhao, H., Nov 1 2013, In : Biotechnology and bioengineering. 110, 11, p. 2874-2881 8 p.

Research output: Contribution to journalArticle

Cellobiose
Yeast
Saccharomyces cerevisiae
Genes
Productivity
2014

SunnyTALEN: A second-generation TALEN system for human genome editing

Sun, N., Bao, Z., Xiong, X. & Zhao, H., Apr 2014, In : Biotechnology and bioengineering. 111, 4, p. 683-691 9 p.

Research output: Contribution to journalArticle

Human Genome
Transcription
Genes
Double-Stranded DNA Breaks
Homologous Recombination
2013

Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13C metabolic flux analysis

Feng, X. & Zhao, H., Sep 1 2013, In : AIChE Journal. 59, 9, p. 3195-3202 8 p.

Research output: Contribution to journalArticle

Metabolic Flux Analysis
Xylose
Metabolism
Yeast
Glucose
2011

Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo

Evans, B. S., Chen, Y., Metcalf, W. W., Zhao, H. & Kelleher, N. L., May 27 2011, In : Chemistry and Biology. 18, 5, p. 601-607 7 p.

Research output: Contribution to journalArticle

Peptide Synthases
Reengineering
Derivatives
Enzymes
Pantoea
2012

Recent advances in targeted genome engineering in mammalian systems

Sun, N., Abil, Z. & Zhao, H., Sep 1 2012, In : Biotechnology Journal. 7, 9, p. 1074-1087 14 p.

Research output: Contribution to journalReview article

Genome
Homologous Recombination
Gene Targeting
Double-Stranded DNA Breaks
Endonucleases

Mutagenic inverted repeats assisted genome engineering (MIRAGE) in saccharomyces cerevisiae: Deletion of gal7

Nair, N. U. & Zhao, H., Jan 20 2012, Microbial Metabolic Engineering: Methods and Protocols. Cheng, Q. (ed.). p. 63-73 11 p. (Methods in Molecular Biology; vol. 834).

Research output: Chapter in Book/Report/Conference proceedingChapter

Synthetic Biology
Metabolic Engineering
Systems Biology
Point Mutation
Saccharomyces cerevisiae
2013

Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analysis

Feng, X. & Zhao, H., Nov 18 2013, In : Microbial cell factories. 12, 1, 114.

Research output: Contribution to journalArticle

Metabolic Flux Analysis
Xylose
Metabolism
Yeast
Saccharomyces cerevisiae

Drug Discovery and Development via Synthetic Biology

Cobb, R. E., Luo, Y., Freestone, T. & Zhao, H., Aug 27 2013, Synthetic Biology. Elsevier Inc., p. 183-206 24 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Synthetic Biology
Drug Discovery
Multigene Family
Biological Products
Erythromycin
2015

Industrial biotechnology: Tools and applications

Zhao, H. & Tan, T., Nov 15 2015, In : Biotechnology Advances. 33, 7, p. 1393-1394 2 p.

Research output: Contribution to journalEditorial

Biotechnology
Industry
2013
Xylose
RNA
Yeast
Saccharomyces cerevisiae
metabolism
2011

Highlights of biocatalysis and biomimetic catalysis

Zhao, H., Sep 2 2011, In : ACS Catalysis. 1, 9, p. 1119-1120 2 p.

Research output: Contribution to journalEditorial

Biomimetics
Catalysis
Biocatalysis

Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering

Desieno, M., Du, J. & Zhao, H., Mar 9 2011, Protein Engineering Handbook, Volume 1 & Volume 2. Wiley-VCH, Vol. 2. p. 777-796 20 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Protein Engineering
Gene therapy
Biosynthesis
Coenzymes
Substrate Specificity
2013

Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

Li, S., Ha, S. J., Kim, H. J., Galazka, J. M., Cate, J. H. D., Jin, Y-S. & Zhao, H., Oct 10 2013, In : Journal of Biotechnology. 168, 1, p. 1-6 6 p.

Research output: Contribution to journalArticle

Cellobiose
Cell growth
Yeast
Fermentation
Glucose
2019

A pathway for isethionate dissimilation in Bacillus krulwichiae

Tong, Y., Wei, Y., Hu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Jan 1 2019, In : Applied and environmental microbiology. 85, 15, e00793-19.

Research output: Contribution to journalArticle

Bacillus (bacteria)
Bacillus
Bacteria
bacterium
bacteria
2018

Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli

Ren, H., Biswas, S., Ho, S., Van Der Donk, W. A. & Zhao, H., Oct 19 2018, In : ACS chemical biology. 13, 10, p. 2966-2972 7 p.

Research output: Contribution to journalArticle

Bacteriocins
Escherichia coli
Genes
Anti-Bacterial Agents
Glycosylation
2011

Drug Discovery and Development by Combinatorial Biosynthesis

Desieno, M. A., Denard, C. A. & Zhao, H., May 25 2011, Enzyme Technologies: Metagenomics, Evolution, Biocatalysis, and Biosynthesis. John Wiley and Sons, p. 251-283 33 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Biosynthesis
Drug Discovery

A new fluorescence complementation biosensor for detection of estrogenic compounds

Mclachlan, M. J., Katzenellenbogen, J. A. & Zhao, H., Dec 1 2011, In : Biotechnology and bioengineering. 108, 12, p. 2794-2803 10 p.

Research output: Contribution to journalArticle

Biosensing Techniques
Biosensors
Estrogens
Fluorescence
Ligands
2012

Investigation of the role of Arg301 identified in the X-ray structure of phosphite dehydrogenase

Hung, J. E., Fogle, E. J., Christman, H. D., Johannes, T. W., Zhao, H., Metcalf, W. W. & Van Der Donk, W. A., May 29 2012, In : Biochemistry. 51, 21, p. 4254-4262 9 p.

Research output: Contribution to journalArticle

Phosphites
Hydroxy Acids
X-Rays
X rays
Oxidoreductases
2011

Combinatorial optimization of a highly efficient xylose utilization pathway in saccharomyces cerevisiae

Du, J., Kim, B. & Zhao, H., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xylose
Combinatorial optimization
Yeast
Pentoses
Enzymes
2016

Engineered minicellulosomes for consolidated bioprocessing

Liang, Y., Ang, E. L. & Zhao, H., Jul 11 2016, Handbook of Carbohydrate-Modifying Biocatalysts. Pan Stanford Publishing Pte. Ltd., Vol. 2. p. 965-992 28 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2010

Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis

Du, J., Li, S. & Zhao, H., Nov 1 2010, In : Molecular BioSystems. 6, 11, p. 2150-2156 7 p.

Research output: Contribution to journalArticle

Neurospora crassa
Pichia
Xylose
Pentoses
Saccharomyces cerevisiae
2016

High-Efficiency Genome Editing of Streptomyces Species by an Engineered CRISPR/Cas System

Wang, Y., Cobb, R. E. & Zhao, H., 2016, Methods in Enzymology. O'Connor, S. E. (ed.). Academic Press Inc., p. 271-284 14 p. (Methods in Enzymology; vol. 575).

Research output: Chapter in Book/Report/Conference proceedingChapter

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
Streptomyces
Genes
Microbial Genome
2013

Directed evolution: Past, present, and future

Cobb, R. E., Chao, R. & Zhao, H., May 1 2013, In : AIChE Journal. 59, 5, p. 1432-1440 9 p.

Research output: Contribution to journalArticle

Screening
Proteins
Biological Phenomena
Mutagenesis
Polymerase chain reaction
2015
Type I Fatty Acid Synthase
Aldehyde Reductase
Oxygenases
Alkanes
Alcohols
2018

Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding

Wang, P., Liang, J., Shi, L. Z., Wang, Y., Zhang, P., Ouyang, M., Preece, D., Peng, Q., Shao, L., Fan, J., Sun, J., Li, S. S., Berns, M. W., Zhao, H. & Wang, Y., Sep 19 2018, In : ACS Photonics. 5, 9, p. 3565-3574 10 p.

Research output: Contribution to journalArticle

Biosensors
Energy transfer
cells
bioinstrumentation
Energy Transfer
2011

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler

Shao, Z., Luo, Y. & Zhao, H., Apr 1 2011, In : Molecular BioSystems. 7, 4, p. 1056-1059 4 p.

Research output: Contribution to journalArticle

Biosynthetic Pathways
Biological Products
Synthetic Biology
Homologous Recombination
DNA
2016

Identification and engineering of n-butanol biosensors by transcriptome analysis in yeast

Shi, S., Ang, E. L., Tan, M. H. & Zhao, H., Jan 1 2016, Metabolic Engineering 11. AIChE, p. 458-459 2 p. (Metabolic Engineering 11).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1-Butanol
Biosensors
Yeast
2017

Programmable DNA-Guided Artificial Restriction Enzymes

Enghiad, B. & Zhao, H., May 19 2017, In : ACS synthetic biology. 6, 5, p. 752-757 6 p.

Research output: Contribution to journalArticle

DNA
Enzymes
Pyrococcus furiosus
Genetic engineering
Cloning
2016

Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization

Wang, M., Yu, C. & Zhao, H., Mar 1 2016, In : Biotechnology and bioengineering. 113, 3, p. 484-491 8 p.

Research output: Contribution to journalArticle

Xylose
Glucose
Neurospora crassa
Facilitative Glucose Transport Proteins
Biofuels
2012

Direct cloning of large genomic sequences

Cobb, R. E. & Zhao, H., May 1 2012, In : Nature Biotechnology. 30, 5, p. 405-406 2 p.

Research output: Contribution to journalShort survey

Exodeoxyribonucleases
Cloning
Escherichia coli Proteins
2017

A New Era of Genome Integration - Simply Cut and Paste!

Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 601-609 9 p.

Research output: Contribution to journalReview article

Ointments
Genes
Homologous Recombination
Genome
Synthetic Biology
2015

Synthetic biology for therapeutic applications

Abil, Z., Xiong, X. & Zhao, H., Feb 2 2015, In : Molecular Pharmaceutics. 12, 2, p. 322-331 10 p.

Research output: Contribution to journalReview article

Synthetic Biology
Bacterial Drug Resistance
Drug Discovery
Communicable Diseases
Neoplasms
2014

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

Lian, J., Si, T., Nair, N. U. & Zhao, H., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, p. 750-760 11 p. (Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Acetyl Coenzyme A
1-Butanol
butanol
Butenes
Yeast
2010

Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis

Choi, Y. S., Johannes, T. W., Simurdiak, M., Shao, Z., Lu, H. & Zhao, H., Feb 8 2010, In : Molecular BioSystems. 6, 2, p. 336-338 3 p.

Research output: Contribution to journalArticle

Streptomyces
Multigene Family
Organism Cloning
Polyketides
spectinabilin
2016
Antibiotics
Yeast
Saccharomyces cerevisiae
Plasmids
Genes
2012

Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome

Sun, J., Wen, F., Si, T., Xu, J. H. & Zhao, H., Jun 1 2012, In : Applied and environmental microbiology. 78, 11, p. 3837-3845 9 p.

Research output: Contribution to journalArticle

Xylans
arabinoxylan
Xylose
xylan
xylose
2013

Transcription activator-like effector nucleases (TALENs): a highly efficient and versatile tool for genome editing.

Sun, N. & Zhao, H., Jul 2013, In : Biotechnology and bioengineering. 110, 7, p. 1811-1821 11 p.

Research output: Contribution to journalReview article

Transcription
Genes
Technology
DNA
DNA sequences
2018

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In : Biotechnology Journal. 13, 9, 1700601.

Research output: Contribution to journalReview article

Clustered Regularly Interspaced Short Palindromic Repeats
Metabolic Engineering
Saccharomyces cerevisiae
Gene Knockout Techniques
Biotechnology

Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes

Tapsin, S., Sun, M., Shen, Y., Zhang, H., Lim, X. N., Susanto, T. T., Yang, S. L., Zeng, G. S., Lee, J., Lezhava, A., Ang, E. L., Zhang, L. H., Wang, Y., Zhao, H., Nagarajan, N. & Wan, Y., Dec 1 2018, In : Nature communications. 9, 1, 1289.

Research output: Contribution to journalArticle

prokaryotes
Nucleotide Aptamers
eukaryotes
Flavin Mononucleotide
genome

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In : Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.

Research output: Contribution to journalReview article

Biocatalysis
Enzymes
Pharmaceutical Preparations
Oxidoreductases
Protein Engineering
2017

Automated multiplex genome-scale engineering in yeast

Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., Jan 1 2017, In : Nature communications. 8, 15187.

Research output: Contribution to journalArticle

genome
yeast
Yeast
Genes
Yeasts
2013

Synthetic Biology

Zhao, H., Jan 1 2013, Elsevier Inc.

Research output: Book/ReportBook

Synthetic Biology
Biological systems
Cytology
Artificial Cells
Molecular biology
2017

Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring

Freestone, T. S., Ju, K. S., Wang, B. & Zhao, H., Feb 17 2017, In : ACS synthetic biology. 6, 2, p. 217-223 7 p.

Research output: Contribution to journalArticle

Phosphonoacetic Acid
Multigene Family
Biological Products
Genes
Acids
2015

Direct observation of TALE protein dynamics reveals a two-state search mechanism

Cuculis, L., Abil, Z., Zhao, H. & Schroeder, C. M., Jun 1 2015, In : Nature communications. 6, 7277.

Research output: Contribution to journalArticle

effectors
Transcription
Observation
proteins
deoxyribonucleic acid
2012

Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration

Zou, Y., Zhang, H., Brunzelle, J. S., Johannes, T. W., Woodyer, R., Hung, J. E., Nair, N., van der Donk, W. A., Zhao, H. & Nair, S. K., May 29 2012, In : Biochemistry. 51, 21, p. 4263-4270 8 p.

Research output: Contribution to journalArticle

Niacinamide
Regeneration
Crystal structure
NAD
Phosphites
2014

FairyTALE: A high-throughput TAL effector synthesis platform

Liang, J., Chao, R., Abil, Z., Bao, Z. & Zhao, H., Feb 21 2014, In : ACS synthetic biology. 3, 2, p. 67-73 7 p.

Research output: Contribution to journalArticle

Transcription
Throughput
DNA sequences
Robotics
Transcription Activator-Like Effectors
2013

Modular assembly of designer Puf proteins for specific post-transcriptional regulation of endogenous RNA

Abil, Z. & Zhao, H., Jan 1 2013, Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

RNA
Proteins
proteins