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  • Huimin Zhao
2020

A genetic toolbox for metabolic engineering of Issatchenkia orientalis

Cao, M., Fatma, Z., Song, X., Hsieh, P. H., Tran, V. G., Lyon, W. L., Sayadi, M., Shao, Z., Yoshikuni, Y. & Zhao, H., May 2020, In : Metabolic Engineering. 59, p. 87-97 11 p.

Research output: Contribution to journalArticle

A mass spectrometry-based high-throughput screening method for engineering fatty acid synthases with improved production of medium-chain fatty acids

Xue, P., Si, T., Mishra, S., Zhang, L., Choe, K., Sweedler, J. V. & Zhao, H., Jul 1 2020, In : Biotechnology and bioengineering. 117, 7, p. 2131-2138 8 p.

Research output: Contribution to journalArticle

A New Biosensor for Stilbenes and a Cannabinoid Enabled by Genome Mining of a Transcriptional Regulator

Sun, H., Zhao, H. & Ang, E. L., Apr 17 2020, In : ACS synthetic biology. 9, 4, p. 698-705 8 p.

Research output: Contribution to journalArticle

A Pathway for Degradation of Uracil to Acetyl Coenzyme A in Bacillus megaterium

Zhu, D., Wei, Y., Yin, J., Liu, D., Ang, E. L., Zhao, H. & Zhang, Y., Mar 18 2020, In : Applied and environmental microbiology. 86, 7

Research output: Contribution to journalArticle

Biosynthetic engineering of the antifungal, anti-MRSA auroramycin

Yeo, W. L., Heng, E., Tan, L. L., Lim, Y. W., Ching, K. C., Tsai, D. J., Jhang, Y. W., Lauderdale, T. L., Shia, K. S., Zhao, H., Ang, E. L., Zhang, M. M., Lim, Y. H. & Wong, F. T., Jan 6 2020, In : Microbial cell factories. 19, 1, 3.

Research output: Contribution to journalArticle

Open Access

Biosystems design by directed evolution

Wang, Y., Yu, X. & Zhao, H., Mar 1 2020, In : AIChE Journal. 66, 3, e16716.

Research output: Contribution to journalReview article

Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways

Ren, H., Shi, C. & Zhao, H., Jan 24 2020, In : iScience. 23, 1, 100795.

Research output: Contribution to journalReview article

Open Access

Divalent cations promote TALE DNA-binding specificity

Cuculis, L., Zhao, C., Abil, Z., Zhao, H., Shukla, D. & Schroeder, C. M., Feb 20 2020, In : Nucleic acids research. 48, 3, p. 1406-1422 17 p.

Research output: Contribution to journalArticle

Open Access

DNA punch cards for storing data on native DNA sequences via enzymatic nicking

Tabatabaei, S. K., Wang, B., Athreya, N. B. M., Enghiad, B., Hernandez, A. G., Fields, C. J., Leburton, J. P., Soloveichik, D., Zhao, H. & Milenkovic, O., Dec 1 2020, In : Nature communications. 11, 1, 1742.

Research output: Contribution to journalArticle

Open Access

Emerging molecular biology tools and strategies for engineering natural product biosynthesis

Xu, W., Klumbys, E., Ang, E. L. & Zhao, H., Jun 2020, In : Metabolic Engineering Communications. 10, e00108.

Research output: Contribution to journalReview article

Open Access

Identification and Characterization of Citrus Peel Uronic Acid Oxidase

Wei, Y., Tan, Y. L., Ang, E. L. & Zhao, H., Mar 16 2020, In : ChemBioChem. 21, 6, p. 797-800 4 p.

Research output: Contribution to journalArticle

Integrating biocatalysis with chemocatalysis for selective transformations

Huang, X., Cao, M. & Zhao, H., Apr 2020, In : Current Opinion in Chemical Biology. 55, p. 161-170 10 p.

Research output: Contribution to journalReview article

Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution

Xue, P., Si, T. & Zhao, H., Jan 1 2020, (Accepted/In press) Methods in Enzymology. Academic Press Inc., (Methods in Enzymology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Photoenzymatic enantioselective intermolecular radical hydroalkylation

Huang, X., Wang, B., Wang, Y., Jiang, G., Feng, J. & Zhao, H., Jan 1 2020, (Accepted/In press) In : Nature.

Research output: Contribution to journalArticle

Recent advances in domesticating non-model microorganisms

Fatma, Z., Schultz, J. C. & Zhao, H., Jan 1 2020, (Accepted/In press) In : Biotechnology Progress.

Research output: Contribution to journalReview article

Towards oilcane: Engineering hyperaccumulation of triacylglycerol into sugarcane stems

Parajuli, S., Kannan, B., Karan, R., Sanahuja, G., Liu, H., Garcia-Ruiz, E., Kumar, D., Singh, V., Zhao, H., Long, S., Shanklin, J. & Altpeter, F., Jan 1 2020, (Accepted/In press) In : GCB Bioenergy.

Research output: Contribution to journalArticle

Open Access

Unleashing the power of energy storage: Engineering β-oxidation pathways for polyketide production

Wang, B. & Zhao, H., Mar 2020, In : Synthetic and Systems Biotechnology. 5, 1, p. 21-22 2 p.

Research output: Contribution to journalComment/debate

Open Access
2019

A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In : Metabolic Engineering Communications. 9, e00101.

Research output: Contribution to journalArticle

Open Access

A continuing career in biocatalysis: Frances H. Arnold

Fasan, R., Jennifer Kan, S. B. & Zhao, H., Nov 1 2019, In : ACS Catalysis. 9, 11, p. 9775-9788 14 p.

Research output: Contribution to journalArticle

Activation of silent biosynthetic gene clusters using transcription factor decoys

Wang, B., Guo, F., Dong, S. H. & Zhao, H., Feb 1 2019, In : Nature chemical biology. 15, 2, p. 111-114 4 p.

Research output: Contribution to journalArticle

A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium

Xing, M., Wei, Y., Hua, G., Li, M., Nanjaraj Urs, A. N., Wang, F., Hu, Y., Zhai, W., Liu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Aug 15 2019, In : Biochemical Journal. 476, 15, p. 2271-2279 9 p.

Research output: Contribution to journalArticle

An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

Yu, Y., Guo, Y., Tian, Q., Lan, Y., Yeh, H., Zhang, M., Tasan, I., Jain, S. & Zhao, H., Dec 23 2019, (Accepted/In press) In : Nature chemical biology. 16, 4, p. 387-390 4 p.

Research output: Contribution to journalArticle

An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine

Yin, J., Wei, Y., Liu, D., Hu, Y., Lu, Q., Lui Ang, E., Zhao, H. & Zhang, Y., Oct 25 2019, In : Journal of Biological Chemistry. 294, 43, p. 15662-15671 10 p.

Research output: Contribution to journalArticle

A pathway for isethionate dissimilation in Bacillus krulwichiae

Tong, Y., Wei, Y., Hu, Y., Ang, E. L., Zhao, H. & Zhang, Y., Jan 1 2019, In : Applied and environmental microbiology. 85, 15, e00793-19.

Research output: Contribution to journalArticle

Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca

Zhou, Y., Wei, Y., Lin, L., Xu, T., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In : Biochemical Journal. 476, 4, p. 733-746 14 p.

Research output: Contribution to journalArticle

Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense

Li, M., Wei, Y., Yin, J., Lin, L., Zhou, Y., Hua, G., Cao, P., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In : Biochemical Journal. 476, 11, p. 1605-1619 15 p.

Research output: Contribution to journalArticle

Building a global alliance of biofoundries

Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., Dec 1 2019, In : Nature communications. 10, 1, 2040.

Research output: Contribution to journalComment/debate

Open Access

Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes

Yeo, W. L., Heng, E., Tan, L. L., Lim, Y. W., Lim, Y. H., Hoon, S., Zhao, H., Zhang, M. M. & Wong, F. T., Jan 1 2019, In : Biotechnology and bioengineering. 116, 9, p. 2330-2338 9 p.

Research output: Contribution to journalArticle

Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098

Nguyen, K., DeSieno, M. A., Bae, B., Johannes, T. W., Cobb, R. E., Zhao, H. & Nair, S. K., Jan 1 2019, In : Organic and Biomolecular Chemistry. 17, 6, p. 1506-1518 13 p.

Research output: Contribution to journalArticle

Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification

Wen, F., Smith, M. R. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 213-234 22 p. (Methods in Molecular Biology; vol. 2024).

Research output: Chapter in Book/Report/Conference proceedingChapter

Delta integration CRISPR-Cas (Di-CRISPR) in saccharomyces cerevisiae

Shi, S., Liang, Y., Ang, E. L. & Zhao, H., Jan 1 2019, Methods in Molecular Biology. Humana Press Inc., p. 73-91 19 p. (Methods in Molecular Biology; vol. 1927).

Research output: Chapter in Book/Report/Conference proceedingChapter

Development of a CRISPR/Cas9-based tool for gene deletion in Issatchenkia orientalis

Tran, V. G., Cao, M., Fatma, Z., Song, X. & Zhao, H., May 1 2019, In : mSphere. 4, 3, e00345-19.

Research output: Contribution to journalArticle

Open Access

Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides

Schultz, J. C., Cao, M. & Zhao, H., Aug 2019, In : Biotechnology and bioengineering. 116, 8, p. 2103-2109 7 p.

Research output: Contribution to journalArticle

Highly Efficient Single-Pot Scarless Golden Gate Assembly

Hamedirad, M., Weisberg, S., Chao, R., Lian, J. & Zhao, H., May 17 2019, In : ACS synthetic biology. 8, 5, p. 1047-1054 8 p.

Research output: Contribution to journalArticle

Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense

Zhou, Y., Wei, Y., Nanjaraj Urs, A. N., Lin, L., Xu, T., Hu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Jan 1 2019, In : Bioscience Reports. 39, 6, Y.

Research output: Contribution to journalArticle

Open Access

Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

Lian, J., Schultz, C., Cao, M., HamediRad, M. & Zhao, H., Dec 1 2019, In : Nature communications. 10, 1, 5794.

Research output: Contribution to journalArticle

Open Access

Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria

Xing, M., Wei, Y., Zhou, Y., Zhang, J., Lin, L., Hu, Y., Hua, G., N. Nanjaraj Urs, A., Liu, D., Wang, F., Guo, C., Tong, Y., Li, M., Liu, Y., Ang, E. L., Zhao, H., Yuchi, Z. & Zhang, Y., Dec 1 2019, In : Nature communications. 10, 1, 1609.

Research output: Contribution to journalArticle

Open Access

Towards a fully automated algorithm driven platform for biosystems design

HamediRad, M., Chao, R., Weisberg, S., Lian, J., Sinha, S. & Zhao, H., Nov 13 2019, In : Nature communications. 10, 1, 5150.

Research output: Contribution to journalArticle

Open Access
2018

A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

Sun, H., Zhao, H. & Ang, E. L., Jan 1 2018, In : Chemical Communications. 54, 68, p. 9458-9461 4 p.

Research output: Contribution to journalArticle

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In : Biotechnology Journal. 13, 9, 1700601.

Research output: Contribution to journalReview article

Applying advanced dna assembly methods to generate pathway libraries

Eriksen, D. T., Chao, R. & Zhao, H., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 331-347 17 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation

Lim, Y. H., Wong, F. T., Yeo, W. L., Ching, K. C., Lim, Y. W., Heng, E., Chen, S., Tsai, D. J., Lauderdale, T. L., Shia, K. S., Ho, Y. S., Hoon, S., Ang, E. L., Zhang, M. M. & Zhao, H., Aug 16 2018, In : ChemBioChem. 19, 16, p. 1716-1719 4 p.

Research output: Contribution to journalArticle

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In : Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.

Research output: Contribution to journalReview article

Cooperative asymmetric reactions combining photocatalysis and enzymatic catalysis

Litman, Z. C., Wang, Y., Zhao, H. & Hartwig, J. F., Aug 16 2018, In : Nature. 560, 7718, p. 355-359 5 p.

Research output: Contribution to journalArticle

CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci

Tasan, I., Sustackova, G., Zhang, L., Kim, J., Sivaguru, M., HamediRad, M., Wang, Y., Genova, J., Ma, J., Belmont, A. S. & Zhao, H., Jan 1 2018, In : Nucleic acids research. 46, 17, e100.

Research output: Contribution to journalArticle

Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface

Limsakul, P., Peng, Q., Wu, Y., Allen, M. E., Liang, J., Remacle, A. G., Lopez, T., Ge, X., Kay, B. K., Zhao, H., Strongin, A. Y., Yang, X. L., Lu, S. & Wang, Y., Apr 19 2018, In : Cell chemical biology. 25, 4, p. 370-379.e4

Research output: Contribution to journalArticle

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains

Lian, J., Bao, Z., Hu, S. & Zhao, H., Jun 2018, In : Biotechnology and bioengineering. 115, 6, p. 1630-1635 6 p.

Research output: Contribution to journalArticle

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J. & Zhao, H., Jul 1 2018, In : Nature Biotechnology. 36, 6, p. 505-508 4 p.

Research output: Contribution to journalArticle

Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes

Tapsin, S., Sun, M., Shen, Y., Zhang, H., Lim, X. N., Susanto, T. T., Yang, S. L., Zeng, G. S., Lee, J., Lezhava, A., Ang, E. L., Zhang, L. H., Wang, Y., Zhao, H., Nagarajan, N. & Wan, Y., Dec 1 2018, In : Nature communications. 9, 1, 1289.

Research output: Contribution to journalArticle