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  • Huimin Zhao
2017

A New Era of Genome Integration - Simply Cut and Paste!

Liu, Z., Liang, Y., Ang, E. L. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 601-609 9 p.

Research output: Contribution to journalReview article

A plug-and-play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae

Ren, H., Hu, P. & Zhao, H., Aug 2017, In : Biotechnology and bioengineering. 114, 8, p. 1847-1854 8 p.

Research output: Contribution to journalArticle

A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis

Xiong, X., Zhang, Y., Yan, J., Jain, S., Chee, S., Ren, B. & Zhao, H., Jun 16 2017, In : ACS synthetic biology. 6, 6, p. 1034-1042 9 p.

Research output: Contribution to journalArticle

Automated multiplex genome-scale engineering in yeast

Si, T., Chao, R., Min, Y., Wu, Y., Ren, W. & Zhao, H., Jan 1 2017, In : Nature communications. 8, 15187.

Research output: Contribution to journalArticle

Breaking the silence: new strategies for discovering novel natural products

Ren, H., Wang, B. & Zhao, H., Dec 1 2017, In : Current Opinion in Biotechnology. 48, p. 21-27 7 p.

Research output: Contribution to journalReview article

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Lian, J., Hamedirad, M., Hu, S. & Zhao, H., Dec 1 2017, In : Nature communications. 8, 1, 1688.

Research output: Contribution to journalArticle

Combining Rh-Catalyzed Diazocoupling and Enzymatic Reduction to Efficiently Synthesize Enantioenriched 2-Substituted Succinate Derivatives

Wang, Y., Bartlett, M. J., Denard, C. A., Hartwig, J. F. & Zhao, H., Apr 7 2017, In : ACS Catalysis. 7, 4, p. 2548-2552 5 p.

Research output: Contribution to journalArticle

CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Zhang, M. M., Wong, F. T., Wang, Y., Luo, S., Lim, Y. H., Heng, E., Yeo, W. L., Cobb, R. E., Enghiad, B., Ang, E. L. & Zhao, H., Jun 1 2017, In : Nature chemical biology. 13, 6, p. 607-609 3 p.

Research output: Contribution to journalArticle

Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae

Shi, S., Choi, Y. W., Zhao, H., Tan, M. H. & Ang, E. L., Dec 2017, In : Bioresource Technology. 245, p. 1343-1351 9 p.

Research output: Contribution to journalArticle

Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring

Freestone, T. S., Ju, K. S., Wang, B. & Zhao, H., Feb 17 2017, In : ACS synthetic biology. 6, 2, p. 217-223 7 p.

Research output: Contribution to journalArticle

Engineering biological systems using automated biofoundries

Chao, R., Mishra, S., Si, T. & Zhao, H., Jul 2017, In : Metabolic Engineering. 42, p. 98-108 11 p.

Research output: Contribution to journalReview article

Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry

Chao, R., Liang, J., Tasan, I., Si, T., Ju, L. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 678-685 8 p.

Research output: Contribution to journalArticle

Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

Abil, Z., Gumy, L. F., Zhao, H. & Hoogenraad, C. C., Jun 16 2017, In : ACS synthetic biology. 6, 6, p. 950-956 7 p.

Research output: Contribution to journalArticle

Metabolic engineering of oleaginous yeasts for production of fuels and chemicals

Shi, S. & Zhao, H., Nov 8 2017, In : Frontiers in Microbiology. 8, NOV, 2185.

Research output: Contribution to journalReview article

Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators

Bao, Z., Jain, S., Jaroenpuntaruk, V. & Zhao, H., Apr 21 2017, In : ACS synthetic biology. 6, 4, p. 686-693 8 p.

Research output: Contribution to journalArticle

Probing the molecular determinants of fluorinase specificity

Yeo, W. L., Chew, X., Smith, D. J., Chan, K. P., Sun, H., Zhao, H., Lim, Y. H. & Ang, E. L., Jan 1 2017, In : Chemical Communications. 53, 17, p. 2559-2562 4 p.

Research output: Contribution to journalArticle

Programmable DNA-Guided Artificial Restriction Enzymes

Enghiad, B. & Zhao, H., May 19 2017, In : ACS synthetic biology. 6, 5, p. 752-757 6 p.

Research output: Contribution to journalArticle

Strain development by whole-cell directed evolution

Si, T., Lian, J. & Zhao, H., Jan 1 2017, Directed Enzyme Evolution: Advances and Applications. Springer International Publishing, p. 173-200 28 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

SynV and SynX: A story more than DNA synthesis

Ren, H. & Zhao, H., May 1 2017, In : Science China Life Sciences. 60, 5, p. 558-560 3 p.

Research output: Contribution to journalArticle

Targeting Specificity of the CRISPR/Cas9 System

Tasan, I. & Zhao, H., Sep 15 2017, In : ACS synthetic biology. 6, 9, p. 1609-1613 5 p.

Research output: Contribution to journalReview article

Twin-primer non-enzymatic DNA assembly: An efficient and accurate multi-part DNA assembly method

Liang, J., Liu, Z., Low, X. Z., Ang, E. L. & Zhao, H., Jun 1 2017, In : Nucleic acids research. 45, 11, p. e94

Research output: Contribution to journalArticle

Twin-primer non-enzymatic DNA assembly: An efficient and accurate multi-part DNA assembly method

Liang, J., Liu, Z., Ang, E. L. & Zhao, H., Jan 1 2017, Synthetic Biology Conference, SEED 2017: Engineering, Evolution, and Design. AIChE, p. 45-46 2 p. (Synthetic Biology Conference, SEED 2017: Engineering, Evolution, and Design).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Using natural products for drug discovery: the impact of the genomics era

Zhang, M. M., Qiao, Y., Ang, E. L. & Zhao, H., May 4 2017, In : Expert Opinion on Drug Discovery. 12, 5, p. 475-487 13 p.

Research output: Contribution to journalReview article

2018

A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

Sun, H., Zhao, H. & Ang, E. L., Jan 1 2018, In : Chemical Communications. 54, 68, p. 9458-9461 4 p.

Research output: Contribution to journalArticle

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Lian, J., HamediRad, M. & Zhao, H., Sep 2018, In : Biotechnology Journal. 13, 9, 1700601.

Research output: Contribution to journalReview article

Applying advanced dna assembly methods to generate pathway libraries

Eriksen, D. T., Chao, R. & Zhao, H., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 331-347 17 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR-Cas9 Activation

Lim, Y. H., Wong, F. T., Yeo, W. L., Ching, K. C., Lim, Y. W., Heng, E., Chen, S., Tsai, D. J., Lauderdale, T. L., Shia, K. S., Ho, Y. S., Hoon, S., Ang, E. L., Zhang, M. M. & Zhao, H., Aug 16 2018, In : ChemBioChem. 19, 16, p. 1716-1719 4 p.

Research output: Contribution to journalArticle

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates

Sun, H., Zhang, H., Ang, E. L. & Zhao, H., Apr 1 2018, In : Bioorganic and Medicinal Chemistry. 26, 7, p. 1275-1284 10 p.

Research output: Contribution to journalReview article

Cooperative asymmetric reactions combining photocatalysis and enzymatic catalysis

Litman, Z. C., Wang, Y., Zhao, H. & Hartwig, J. F., Aug 16 2018, In : Nature. 560, 7718, p. 355-359 5 p.

Research output: Contribution to journalArticle

CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci

Tasan, I., Sustackova, G., Zhang, L., Kim, J., Sivaguru, M., HamediRad, M., Wang, Y., Genova, J., Ma, J., Belmont, A. S. & Zhao, H., Jan 1 2018, In : Nucleic acids research. 46, 17, e100.

Research output: Contribution to journalArticle

Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface

Limsakul, P., Peng, Q., Wu, Y., Allen, M. E., Liang, J., Remacle, A. G., Lopez, T., Ge, X., Kay, B. K., Zhao, H., Strongin, A. Y., Yang, X. L., Lu, S. & Wang, Y., Apr 19 2018, In : Cell chemical biology. 25, 4, p. 370-379.e4

Research output: Contribution to journalArticle

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains

Lian, J., Bao, Z., Hu, S. & Zhao, H., Jun 2018, In : Biotechnology and bioengineering. 115, 6, p. 1630-1635 6 p.

Research output: Contribution to journalArticle

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J. & Zhao, H., Jul 1 2018, In : Nature Biotechnology. 36, 6, p. 505-508 4 p.

Research output: Contribution to journalArticle

Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes

Tapsin, S., Sun, M., Shen, Y., Zhang, H., Lim, X. N., Susanto, T. T., Yang, S. L., Zeng, G. S., Lee, J., Lezhava, A., Ang, E. L., Zhang, L. H., Wang, Y., Zhao, H., Nagarajan, N. & Wan, Y., Dec 1 2018, In : Nature communications. 9, 1, 1289.

Research output: Contribution to journalArticle

Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A. N., Liu, Y., Ang, E. L., Zhao, S., Zhao, H. & Zhang, Y., Dec 1 2018, In : Nature communications. 9, 1, 4224.

Research output: Contribution to journalArticle

Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction

Singh, R. K., Singh, R., Sivakumar, D., Kondaveeti, S., Kim, T., Li, J., Sung, B. H., Cho, B. K., Kim, D. R., Kim, S. C., Kalia, V. C., Zhang, Y. H. P. J., Zhao, H., Kang, Y. C. & Lee, J. K., Dec 7 2018, In : ACS Catalysis. 8, 12, p. 11085-11093 9 p.

Research output: Contribution to journalArticle

In vivo biosensors: mechanisms, development, and applications

Shi, S., Ang, E. L. & Zhao, H., Jul 1 2018, In : Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 491-516 26 p.

Research output: Contribution to journalArticle

Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion

Schultz, C., Lian, J. & Zhao, H., Jan 1 2018, Methods in Enzymology. Scrutton, N. (ed.). Academic Press Inc., p. 265-276 12 p. (Methods in Enzymology; vol. 608).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pathway design, engineering, and optimization

Garcia-Ruiz, E., HamediRad, M. & Zhao, H., Jan 1 2018, Advances in Biochemical Engineering/Biotechnology. Springer Science and Business Media Deutschland GmbH, (Advances in Biochemical Engineering/Biotechnology; vol. 162).

Research output: Chapter in Book/Report/Conference proceedingChapter

Quantifying the effects of pollen nutrition on honey bee queen egg laying with a new laboratory system

Fine, J. D., Shpigler, H. Y., Ray, A. M., Beach, N. J., Sankey, A. L., Cash-Ahmed, A., Huang, Z. Y., Astrauskaite, I., Chao, R., Zhao, H. & Robinson, G. E., Sep 2018, In : PloS one. 13, 9, e0203444.

Research output: Contribution to journalArticle

Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli

Ren, H., Biswas, S., Ho, S., Van Der Donk, W. A. & Zhao, H., Oct 19 2018, In : ACS chemical biology. 13, 10, p. 2966-2972 7 p.

Research output: Contribution to journalArticle

Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli

Si, T., Tian, Q., Min, Y., Zhang, L., Sweedler, J. V., Van Der Donk, W. A. & Zhao, H., Sep 26 2018, In : Journal of the American Chemical Society. 140, 38, p. 11884-11888 5 p.

Research output: Contribution to journalArticle

Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications

Lian, J., Mishra, S. & Zhao, H., Nov 2018, In : Metabolic Engineering. 50, p. 85-108 24 p.

Research output: Contribution to journalReview article

Recent developments in the application of P450 based biocatalysts

Wei, Y., Ang, E. L. & Zhao, H., Apr 2018, In : Current Opinion in Chemical Biology. 43, p. 1-7 7 p.

Research output: Contribution to journalReview article

RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

HamediRad, M., Lian, J., Li, H. & Zhao, H., Jun 2018, In : Biotechnology and bioengineering. 115, 6, p. 1552-1560 9 p.

Research output: Contribution to journalArticle

Synthetic biology advances and applications in the biotechnology industry: a perspective

Katz, L., Chen, Y. Y., Gonzalez, R., Peterson, T. C., Zhao, H. & Baltz, R. H., Jul 1 2018, In : Journal of Industrial Microbiology and Biotechnology. 45, 7, p. 449-461 13 p.

Research output: Contribution to journalArticle

Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding

Wang, P., Liang, J., Shi, L. Z., Wang, Y., Zhang, P., Ouyang, M., Preece, D., Peng, Q., Shao, L., Fan, J., Sun, J., Li, S. S., Berns, M. W., Zhao, H. & Wang, Y., Sep 19 2018, In : ACS Photonics. 5, 9, p. 3565-3574 10 p.

Research output: Contribution to journalArticle

2019

A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

Dinh, H. V., Suthers, P. F., Chan, S. H. J., Shen, Y., Xiao, T., Deewan, A., Jagtap, S. S., Zhao, H., Rao, C. V., Rabinowitz, J. D. & Maranas, C. D., Dec 2019, In : Metabolic Engineering Communications. 9, e00101.

Research output: Contribution to journalArticle

Open Access