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2010

Application of fragment-based drug discovery to membrane proteins: Identification of ligands of the integral membrane enzyme DsbB

Früh, V., Zhou, Y., Chen, D., Loch, C., Ab, E., Grinkova, Y. N., Verheij, H., Sligar, S. G., Bushweller, J. H. & Siegal, G., Aug 27 2010, In : Chemistry and Biology. 17, 8, p. 881-891 11 p.

Research output: Contribution to journalArticle

Drug Discovery
Membrane Proteins
Ligands
Membranes
Enzymes

Electron transfer in the complex of membrane-bound human cytochrome P450 3A4 with the flavin domain of P450BM-3: The effect of oligomerization of the heme protein and intermittent modulation of the spin equilibrium

Davydov, D. R., Sineva, E. V., Sistla, S., Davydova, N. Y., Frank, D. J., Sligar, S. & Halpert, J. R., Mar 1 2010, In : Biochimica et Biophysica Acta - Bioenergetics. 1797, 3, p. 378-390 13 p.

Research output: Contribution to journalArticle

Hemeproteins
Cytochrome P-450 CYP3A
Oligomerization
Modulation
Electrons

Elliptical structure of phospholipid bilayer nanodiscs encapsulated by scaffold proteins: Casting the roles of the lipids and the protein

Skar-Gislinge, N., Simonsen, J. B., Mortensen, K., Feidenhans'l, R., Sligar, S., Lindberg Møller, B., Bjørnholm, T. & Arleth, L., Oct 6 2010, In : Journal of the American Chemical Society. 132, 39, p. 13713-13722 10 p.

Research output: Contribution to journalArticle

Phospholipids
Scaffolds (biology)
phospholipid
Scaffolds
Lipids

Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers

Grinkova, Y. V., Denisov, I. G. & Sligar, S., Nov 1 2010, In : Protein Engineering, Design and Selection. 23, 11, p. 843-848 6 p.

Research output: Contribution to journalArticle

Lipid bilayers
Apolipoprotein A-I
Lipid Bilayers
Scaffolds (biology)
Scaffolds

Functional reconstitution of monomeric CYP3A4 with multiple cytochrome P450 reductase molecules in Nanodiscs

Grinkova, Y. V., Denisov, I. G. & Sligar, S., Jul 1 2010, In : Biochemical and Biophysical Research Communications. 398, 2, p. 194-198 5 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
NADPH-Ferrihemoprotein Reductase
Cardiopulmonary Resuscitation
Molecules
Cytochrome P-450 Enzyme System

Glimpsing the critical intermediate in cytochrome P450 oxidations

Sligar, S., Nov 12 2010, In : Science. 330, 6006, p. 924-925 2 p.

Research output: Contribution to journalShort survey

Cytochrome P-450 Enzyme System
Hydrogen
Carbon

Lipid-protein correlations in nanoscale phospholipid bilayers determined by solid-state nuclear magnetic resonance

Kijac, A., Shih, A. Y., Nieuwkoop, A. J., Schulten, K., Sligar, S. G. & Rienstra, C. M., Nov 2 2010, In : Biochemistry. 49, 43, p. 9190-9198 9 p.

Research output: Contribution to journalArticle

Phospholipids
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Lipids
Membrane Proteins

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

Grashoff, C., Hoffman, B. D., Brenner, M. D., Zhou, R., Parsons, M., Yang, M. T., McLean, M. A., Sligar, S. G., Chen, C. S., Ha, T. & Schwartz, M. A., Jul 8 2010, In : Nature. 466, 7303, p. 263-266 4 p.

Research output: Contribution to journalArticle

Vinculin
Focal Adhesions
Biosensing Techniques
Cellular Structures
Actin Cytoskeleton

Membrane protein assembly into Nanodiscs

Bayburt, T. H. & Sligar, S., May 1 2010, In : FEBS Letters. 584, 9, p. 1721-1727 7 p.

Research output: Contribution to journalReview article

Membrane Proteins
Phospholipids
Micelles
Liposomes
Detergents

Nanomechanical detection of cholera toxin using microcantilevers functionalized with ganglioside nanodiscs

Tark, S. H., Das, A., Sligar, S. & Dravid, V. P., Oct 29 2010, In : Nanotechnology. 21, 43, 435502.

Research output: Contribution to journalArticle

Gangliosides
Cholera Toxin
Proteins
Light interference
Lipid bilayers

Photoelectrochemical complexes for solar energy conversion that chemically and autonomously regenerate

Ham, M. H., Choi, J. H., Boghossian, A. A., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S. G., Wraight, C. A. & Strano, M. S., Nov 1 2010, In : Nature Chemistry. 2, 11, p. 929-936 8 p.

Research output: Contribution to journalArticle

Energy conversion
Surface-Active Agents
Solar energy
Carbon Nanotubes
Surface active agents

Recreation of the terminal events in physiological integrin activation

Ye, F., Hu, G., Taylor, D., Ratnikov, B., Bobkov, A. A., McLean, M. A., Sligar, S., Taylor, K. A. & Ginsberg, M. H., Dec 11 2010, In : Journal of Cell Biology. 188, 1, p. 157-173 17 p.

Research output: Contribution to journalArticle

Recreation
Integrins
Talin
Extracellular Matrix
Cell Adhesion

Resonance Raman studies on mammalian cytochromes P450

Mak, P. J., Kincaid, J. R., Denisov, I. G., Sligar, S. G., Zhang, H. & Hollenberg, P. F., Dec 14 2010, XXII International Conference on Raman Spectroscopy, ICORS 2010. p. 890-891 2 p. (AIP Conference Proceedings; vol. 1267).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

cytochromes
2011

(609d) Photoelectrochemical complexes for solar energy conversion that dynamically, reversibly and autonomously self-assemble

Boghossian, A. A., Choi, J. H., Ham, M. H., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S., Wraight, C. A. & Strano, M., Jan 1 2011, Nanomaterials for Energy Applications - Topical Conference at the 2011 AIChE Annual Meeting. AIChE, p. 92-93 2 p. (Nanomaterials for Energy Applications - Topical Conference at the 2011 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

solar energy
Energy conversion
Solar energy

Analysis of heterotropic cooperativity in cytochrome P450 3A4 using α-Naphthoflavone and testosterone

Frank, D. J., Denisov, I. G. & Sligar, S. G., Feb 18 2011, In : Journal of Biological Chemistry. 286, 7, p. 5540-5545 6 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
Testosterone
Substrates
Enzyme kinetics
Metabolome

Anomalous transitions of DODAB using fast scanning liquid calorimetry

De La Rama, L. P., Hu, L., Efremov, M. Y., Olson, E. A., Nealey, P. F., McLean, M. A., Sligar, S. & Allen, L. H., Aug 10 2011, In : Thermochimica Acta. 522, 1-2, p. 72-76 5 p.

Research output: Contribution to journalArticle

ammonium bromides
Calorimetry
Calorimeters
heat measurement
Scanning

Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins

Goldsmith, B. R., Mitala, J. J., Josue, J., Castro, A., Lerner, M. B., Bayburt, T. H., Khamis, S. M., Jones, R. A., Brand, J. G., Sligar, S. G., Luetje, C. W., Gelperin, A., Rhodes, P. A., Discher, B. M. & Johnson, A. T. C., Jul 26 2011, In : ACS Nano. 5, 7, p. 5408-5416 9 p.

Research output: Contribution to journalArticle

Odorant Receptors
Nanoelectronics
biomimetics
Biomimetics
G-Protein-Coupled Receptors

Cytochromes P450 in nanodiscs

Denisov, I. G. & Sligar, S., Jan 1 2011, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1814, 1, p. 223-229 7 p.

Research output: Contribution to journalReview article

Cytochrome P-450 Enzyme System
Membranes
Kinetics
Viral Antigens
Biosynthesis

Defining CYP3A4 structural responses to substrate binding. raman spectroscopic studies of a nanodisc-incorporated mammalian cytochrome P450

Mak, P. J., Denisov, I. G., Grinkova, Y. V., Sligar, S. & Kincaid, J. R., Feb 9 2011, In : Journal of the American Chemical Society. 133, 5, p. 1357-1366 10 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
structural response
Bromocriptine
Cytochrome P-450 Enzyme System
cytochrome

Investigation of the low frequency dynamics of heme proteins: Native and mutant cytochrome P450cam and redox partner complexes

Karunakaran, V., Denisov, I., Sligar, S. G. & Champion, P. M., May 12 2011, In : Journal of Physical Chemistry B. 115, 18, p. 5665-5677 13 p.

Research output: Contribution to journalArticle

Camphor 5-Monooxygenase
Hemeproteins
cytochromes
Camphor
camphor

Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding

Bayburt, T. H., Vishnivetskiy, S. A., McLean, M. A., Morizumi, T., Huang, C. C., Tesmer, J. J. G., Ernst, O. P., Sligar, S. & Gurevich, V. V., Jan 14 2011, In : Journal of Biological Chemistry. 286, 2, p. 1420-1428 9 p.

Research output: Contribution to journalArticle

G-Protein-Coupled Receptor Kinase 1
Arrestin
Phosphorylation
Rhodopsin
Transducin

Photoelectrochemical complexes for solar energy conversion that dynamically, reversibly and autonomously self-assemble

Boghossian, A. A., Choi, J. H., Ham, M. H., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S., Wraight, C. A. & Strano, M., Jan 1 2011, Energy and Transport Processes - Core Programming Topic at the 2011 AIChE Annual Meeting. AIChE, p. 436-437 2 p. (Energy and Transport Processes - Core Programming Topic at the 2011 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

solar energy
Energy conversion
Solar energy

Photoelectrochemical complexes for solar energy conversion that dynamically, reversibly and autonomously self-assemble

Boghossian, A. A., Choi, J. H., Ham, M. H., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S., Wraight, C. A. & Strano, M., Dec 1 2011, 11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings. (11AIChE - 2011 AIChE Annual Meeting, Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Single-walled carbon nanotubes (SWCN)
Energy conversion
Solar energy
Surface-Active Agents
Surface active agents

Photoelectrochemical complexes for solar energy conversion that dynamically, reversibly and autonomously self-assemble

Boghossian, A. A., Choi, J. H., Ham, M. H., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S., Wraight, C. A. & Strano, M., Jan 1 2011, Sustainable Engineering Forum: Core Programming Topic at the 2011 AIChE Annual Meeting. AIChE, p. 1161-1162 2 p. (Sustainable Engineering Forum: Core Programming Topic at the 2011 AIChE Annual Meeting; vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

solar energy
Energy conversion
Solar energy

Photoelectrochemical complexes for solar energy conversion that dynamically, reversibly and autonomously self-Assemble

Boghossian, A. A., Choi, J. H., Ham, M. H., Jeng, E. S., Graff, R. A., Heller, D. A., Chang, A. C., Mattis, A., Bayburt, T. H., Grinkova, Y. V., Zeiger, A. S., Van Vliet, K. J., Hobbie, E. K., Sligar, S., Wraight, C. A. & Strano, M., Jan 1 2011, Fuels and Petrochemicals Division - Core Programming Topic at the 2011 AIChE Annual Meeting. AIChE, p. 349-350 2 p. (Fuels and Petrochemicals Division - Core Programming Topic at the 2011 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

solar energy
Energy conversion
Solar energy

Spectroscopic features of cytochrome P450 reaction intermediates

Luthra, A., Denisov, I. G. & Sligar, S., Mar 1 2011, In : Archives of Biochemistry and Biophysics. 507, 1, p. 26-35 10 p.

Research output: Contribution to journalReview article

Reaction intermediates
Heme
Cytochrome P-450 Enzyme System
Enzymes
Isoenzymes

Temperature derivative spectroscopy to monitor the autoxidation decay of cytochromes P450

Luthra, A., Denisov, I. G. & Sligar, S., Jul 1 2011, In : Analytical chemistry. 83, 13, p. 5394-5399 6 p.

Research output: Contribution to journalArticle

Cytochrome P-450 Enzyme System
Spectroscopy
Derivatives
Camphor 5-Monooxygenase
Hemeproteins
2012

A novel type of allosteric regulation: Functional cooperativity in monomeric proteins

Denisov, I. G. & Sligar, S., Mar 15 2012, In : Archives of Biochemistry and Biophysics. 519, 2, p. 91-102 12 p.

Research output: Contribution to journalReview article

Allosteric Regulation
Cytochrome P-450 Enzyme System
Drug interactions
Xenobiotics
Heme

Cryoradiolysis and cryospectroscopy for studies of heme-oxygen intermediates in cytochromes P450

Denisov, I. G., Grinkova, Y. V. & Sligar, S., Jan 1 2012, Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., p. 375-391 17 p. (Methods in Molecular Biology; vol. 875).

Research output: Chapter in Book/Report/Conference proceedingChapter

Heme
Cytochrome P-450 Enzyme System
Oxygen
Peroxidases
Heme Oxygenase (Decyclizing)

Incorporation of NADH-ubiquinone Oxidoreductase (Mitochondrial Complex I) into Lipid Nanodiscs

Fowler, D. J., Sligar, S., Zickermann, V., Brandt, U., Ruiz, T. & Radermacher, M., Jul 2012, In : Microscopy and Microanalysis. 18, S2, p. 88-89 2 p.

Research output: Contribution to journalArticle

Lipids
lipids
Oxidoreductases
Ubiquinone

Native mass spectrometry characterization of intact nanodisc lipoprotein complexes

Marty, M. T., Zhang, H., Cui, W., Blankenship, R. E., Gross, M. L. & Sligar, S., Nov 6 2012, In : Analytical chemistry. 84, 21, p. 8957-8960 4 p.

Research output: Contribution to journalArticle

Lipoproteins
Mass spectrometry
Lipid bilayers
Lipids
Membrane Proteins

Nonlinear analyte concentration gradients for one-step kinetic analysis employing optical microring resonators

Marty, M. T., Sloan, C. D. K., Bailey, R. C. & Sligar, S., Jul 3 2012, In : Analytical chemistry. 84, 13, p. 5556-5564 9 p.

Research output: Contribution to journalArticle

Optical resonators
Kinetics
Association reactions
Gradient methods
Silicon

Reconstitution of respiratory oxidases in membrane nanodiscs for investigation of proton-coupled electron transfer

Näsvik Öjemyr, L., Von Ballmoos, C., Gennis, R. B., Sligar, S. & Brzezinski, P., Mar 9 2012, In : FEBS Letters. 586, 5, p. 640-645 6 p.

Research output: Contribution to journalReview article

Liposomes
Protons
Oxidoreductases
Electrons
Membranes

Structural differences between soluble and membrane bound cytochrome P450s

Denisov, I. G., Shih, A. Y. & Sligar, S., Mar 1 2012, In : Journal of Inorganic Biochemistry. 108, p. 150-158 9 p.

Research output: Contribution to journalArticle

Cytochromes
Membranes
Heme
Substrates
Propionates

Tissue factor/factor VIIa complex: Role of the membrane surface

Morrissey, J. H., Tajkhorshid, E., Sligar, S. G. & Rienstra, C. M., May 1 2012, In : Thrombosis Research. 129, SUPPL. 2

Research output: Contribution to journalArticle

Factor VIIa
Thromboplastin
Membranes
Factor X
Blood Coagulation

Two copies of the SecY channel and acidic lipids are necessary to activate the SecA translocation ATPase

Dalal, K., Chan, C. S., Sligar, S. G. & Duong, F., Mar 13 2012, In : Proceedings of the National Academy of Sciences of the United States of America. 109, 11, p. 4104-4109 6 p.

Research output: Contribution to journalArticle

Adenosine Triphosphatases
Lipids
Membranes

Ultra-thin layer MALDI mass spectrometry of membrane proteins in nanodiscs

Marty, M. T., Das, A. & Sligar, S., Jan 1 2012, In : Analytical and bioanalytical chemistry. 402, 2, p. 721-729 9 p.

Research output: Contribution to journalArticle

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass spectrometry
Mass Spectrometry
Membrane Proteins
Scaffolds
2013

Characterizing the membrane-bound state of cytochrome P450 3A4: Structure, depth of insertion, and orientation

Baylon, J. L., Lenov, I. L., Sligar, S. G. & Tajkhorshid, E., Jun 12 2013, In : Journal of the American Chemical Society. 135, 23, p. 8542-8551 10 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
cytochrome
membrane
Membranes
Enzymes

Constitutively active rhodopsin mutants causing night blindness are effectively phosphorylated by GRKs but differ in arrestin-1 binding

Vishnivetskiy, S. A., Ostermaier, M. K., Singhal, A., Panneels, V., Homan, K. T., Glukhova, A., Sligar, S., Tesmer, J. J. G., Schertler, G. F. X., Standfuss, J. & Gurevich, V. V., Nov 1 2013, In : Cellular Signalling. 25, 11, p. 2155-2162 8 p.

Research output: Contribution to journalArticle

Night Blindness
Arrestin
Rhodopsin
Opsins
G-Protein-Coupled Receptor Kinase 1

Differential hydrogen bonding in human CYP17 dictates hydroxylation versus lyase chemistry

Gregory, M., Mak, P. J., Sligar, S. G. & Kincaid, J. R., May 10 2013, In : Angewandte Chemie - International Edition. 52, 20, p. 5342-5345 4 p.

Research output: Contribution to journalArticle

Steroid 17-alpha-Hydroxylase
Hydroxylation
Lyases
Hydrogen bonds
Substrates

Interfacing lipid bilayer nanodiscs and silicon photonic sensor arrays for multiplexed protein-lipid and protein-membrane protein interaction screening

Sloan, C. D. K., Marty, M. T., Sligar, S. & Bailey, R. C., Mar 5 2013, In : Analytical chemistry. 85, 5, p. 2970-2976 7 p.

Research output: Contribution to journalArticle

Lipid bilayers
Sensor arrays
Silicon
Photonics
Lipids

Investigations of heme ligation and ligand switching in cytochromes P450 and P420

Sun, Y., Zeng, W., Benabbas, A., Ye, X., Denisov, I., Sligar, S. G., Du, J., Dawson, J. H. & Champion, P. M., Aug 27 2013, In : Biochemistry. 52, 34, p. 5941-5951 11 p.

Research output: Contribution to journalArticle

Heme
Histidine
Carbon Monoxide
Cytochrome P-450 Enzyme System
Ligation

Kinetic solvent isotope effect in human P450 CYP17A1-mediated androgen formation: Evidence for a reactive peroxoanion intermediate

Gregory, M. C., Denisov, I. G., Grinkova, Y. V., Khatri, Y. & Sligar, S. G., Nov 6 2013, In : Journal of the American Chemical Society. 135, 44, p. 16245-16247 3 p.

Research output: Contribution to journalArticle

androgen
Isotopes
Androgens
isotope
kinetics

Nanodiscs as a new tool to examine lipid-protein interactions

Schuler, M. A., Denisov, I. G. & Sligar, S., 2013, Lipid-Protein Interactions: Methods and Protocols. Humana Press Inc., p. 415-433 19 p. (Methods in Molecular Biology; vol. 974).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lipids
Membrane Proteins
Lipid Bilayers
Proteins
Membranes

Nanodiscs as a therapeutic delivery agent: Inhibition of respiratory syncytial virus infection in the lung

Numata, M., Grinkova, Y. V., Mitchell, J. R., Chu, H. W., Sligar, S. G. & Voelker, D. R., Apr 12 2013, In : International journal of nanomedicine. 8, p. 1417-1427 11 p.

Research output: Contribution to journalArticle

Respiratory Syncytial Virus Infections
Apolipoprotein A-I
Phospholipids
Virus Diseases
Viruses

Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes

Luthra, A., Gregory, M., Grinkova, Y. V., Denisov, I. G. & Sligar, S., 2013, Cytochrome P450 Protocols. Humana Press Inc., p. 115-127 13 p. (Methods in Molecular Biology; vol. 987).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cytochrome P-450 Enzyme System
NADPH-Ferrihemoprotein Reductase
Membranes
Cytochrome P-450 CYP3A
Lipid Bilayers

Nanodisc-solubilized membrane protein library reflects the membrane proteome

Marty, M. T., Wilcox, K. C., Klein, W. L. & Sligar, S., Feb 12 2013, In : Analytical and Bioanalytical Chemistry. 405, 12, p. 4009-4016 8 p.

Research output: Contribution to journalArticle

Proteome
Membrane Proteins
Membranes
Libraries
Proteins

Oxidase uncoupling in heme monooxygenases: Human cytochrome P450 CYP3A4 in Nanodiscs

Grinkova, Y. V., Denisov, I. G., McLean, M. A. & Sligar, S., Jan 25 2013, In : Biochemical and Biophysical Research Communications. 430, 4, p. 1223-1227 5 p.

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
Mixed Function Oxygenases
Heme
Cytochrome P-450 Enzyme System
Oxidoreductases
2014

Active site proton delivery and the lyase activity of human CYP17A1

Khatri, Y., Gregory, M. C., Grinkova, Y. V., Denisov, I. G. & Sligar, S., Jan 3 2014, In : Biochemical and Biophysical Research Communications. 443, 1, p. 179-184 6 p.

Research output: Contribution to journalArticle

Lyases
Human Activities
Protons
Catalytic Domain
Carbon

Assembly of an activated rhodopsin-transducin complex in nanoscale lipid bilayers

D'Antona, A. M., Xie, G., Sligar, S. & Oprian, D. D., Jan 14 2014, In : Biochemistry. 53, 1, p. 127-134 8 p.

Research output: Contribution to journalArticle

Transducin
Lipid bilayers
Rhodopsin
Lipid Bilayers
Stoichiometry