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2010

Antibacterial activity of N-pentylpantothenamide is due to inhibition of coenzyme A synthesis

Thomas, J. & Cronan, J. E., Mar 1 2010, In : Antimicrobial Agents and Chemotherapy. 54, 3, p. 1374-1377 4 p.

Research output: Contribution to journalArticle

Acyl Carrier Protein
Coenzyme A
Growth
Proteins
(acyl-carrier-protein) phosphodiesterase

Biotin synthesis begins by hijacking the fatty acid synthetic pathway

Lin, S., Hanson, R. E. & Cronan, J. E., Sep 2010, In : Nature chemical biology. 6, 9, p. 682-688 7 p.

Research output: Contribution to journalArticle

Biotin
Fatty Acids
Acyl Carrier Protein
Pimelic Acids
Esters
Acyl Carrier Protein
Biochemistry
Ligases
butyryl-CoA synthetase

Expression of Vibrio harveyi Acyl-ACP synthetase allows efficient entry of exogenous fatty acids into the Escherichia coli fatty acid and lipid a synthetic pathways

Jiang, Y., Morgan-Kiss, R. M., Campbell, J. W., Chan, C. H. & Cronan, J. E., Feb 2 2010, In : Biochemistry. 49, 4, p. 718-726 9 p.

Research output: Contribution to journalArticle

Vibrio
Escherichia coli
Fatty Acids
Lipids
Nonesterified Fatty Acids

Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function

Volkmann, G., Murphy, P. W., Rowland, E. E., Cronan, J. E., Liu, X. Q., Blouin, C. & Byers, D. M., Mar 19 2010, In : Journal of Biological Chemistry. 285, 12, p. 8605-8614 10 p.

Research output: Contribution to journalArticle

Inteins
Acyl Carrier Protein
Cyclization
Conformations
Protein Unfolding

Lipoic acid synthesis: A new family of octanoyltransferases generally annotated as lipoate protein ligases

Christensen, Q. H. & Cronan, J. E., Nov 23 2010, In : Biochemistry. 49, 46, p. 10024-10036 13 p.

Research output: Contribution to journalArticle

Thioctic Acid
Escherichia coli
Bacillus subtilis
Enzymes
Ligases

Overlapping repressor binding sites result in additive regulation of Escherichia coli FadH by FadR and ArcA

Feng, Y. & Cronan, J. E., Sep 1 2010, In : Journal of bacteriology. 192, 17, p. 4289-4299 11 p.

Research output: Contribution to journalArticle

Cyclic AMP Receptor Protein
Cyclic AMP
Binding Sites
Escherichia coli
Fatty Acids
Transcription
Operon
Biotin
Escherichia coli
Switches

Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis

Wier, A. M., Nyholm, S. V., Mandel, M. J., Massengo-Tiassé, R. P., Schaefer, A. L., Koroleva, I., Splinter-BonDurant, S., Brown, B., Manzella, L., Snir, E., Almabrazi, H., Scheetz, T. E., Bonaldo, M. D. F., Casavant, T. L., Soares, M. B., Cronan, J. E., Reed, J. L., Ruby, E. G. & McFall-Ngai, M. J., Feb 2 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 5, p. 2259-2264 6 p.

Research output: Contribution to journalArticle

Symbiosis
Bacteria
Decapodiformes
Epithelium
Population

Triclosan resistance of Pseudomonas aeruginosa PAO1 is due to FabV, a triclosan-resistant enoyl-acyl carrier protein reductase

Zhu, L., Lin, J., Ma, J., Cronan, J. E. & Wang, H., Feb 1 2010, In : Antimicrobial Agents and Chemotherapy. 54, 2, p. 689-698 10 p.

Research output: Contribution to journalArticle

Triclosan
Acyl Carrier Protein
Pseudomonas aeruginosa
Oxidoreductases
Disinfectants
2011

A complex lipoate utilization pathway in Listeria monocytogenes

Christensen, Q. H., Hagar, J. A., O'Riordan, M. X. D. & Cronan, J. E., Sep 9 2011, In : Journal of Biological Chemistry. 286, 36, p. 31447-31456 10 p.

Research output: Contribution to journalArticle

Listeria
Thioctic Acid
Listeria monocytogenes
Scavenging
Metabolism

A novel amidotransferase required for lipoic acid cofactor assembly in Bacillus subtilis

Christensen, Q. H., Martin, N., Mansilla, M. C., de Mendoza, D. & Cronan, J. E., Apr 1 2011, In : Molecular Microbiology. 80, 2, p. 350-363 14 p.

Research output: Contribution to journalArticle

Thioctic Acid
Bacillus subtilis
Enzymes
Pyruvic Acid
Oxidoreductases

A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis

Martin, N., Christensen, Q. H., Mansilla, M. C., Cronan, J. E. & de Mendoza, D., Apr 1 2011, In : Molecular Microbiology. 80, 2, p. 335-349 15 p.

Research output: Contribution to journalArticle

Thioctic Acid
Bacillus subtilis
Lipoylation
Genes
Ligases

Closing in on complete pathways of biotin biosynthesis

Lin, S. & Cronan, J. E., Jun 1 2011, In : Molecular BioSystems. 7, 6, p. 1811-1821 11 p.

Research output: Contribution to journalReview article

Biotin
Bacillus subtilis
Escherichia coli
Coenzymes
Biosynthetic Pathways

Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters

Feng, Y. & Cronan, J. E., Apr 1 2011, In : Molecular Microbiology. 80, 1, p. 195-218 24 p.

Research output: Contribution to journalArticle

Vibrio cholerae
Unsaturated Fatty Acids
Escherichia coli
Genetic Promoter Regions
Binding Sites

Probing protein dynamics in vivo using backbone cyclization: Bacterial acyl carrier protein as a case study

Volkmann, G., Murphy, P. W., Rowland, E. E., Cronan, J. E. & Byers, D. M., Dec 23 2011, Methods in Protein Biochemistry. Walter de Gruyter GmbH and Co. KG, p. 271-293 23 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Acyl Carrier Protein
acyl carrier protein
Cyclization
case studies
Proteins

Protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism

Hassan, B. H. & Cronan, J. E., Mar 11 2011, In : Journal of Biological Chemistry. 286, 10, p. 8263-8276 14 p.

Research output: Contribution to journalArticle

Keto Acids
Thioctic Acid
Metabolism
Oxidoreductases
Ketoglutarate Dehydrogenase Complex

Synthesis of the α,ω-dicarboxylic acid precursor of biotin by the canonical fatty acid biosynthetic pathway

Cronan, J. E. & Lin, S., Jun 1 2011, In : Current Opinion in Chemical Biology. 15, 3, p. 407-413 7 p.

Research output: Contribution to journalReview article

Dicarboxylic Acids
Pimelic Acids
Biosynthetic Pathways
Biotin
Fatty Acids

The enzyme function initiative

Gerlt, J. A., Allen, K. N., Almo, S. C., Armstrong, R. N., Babbitt, P. C., Cronan, J. E., Dunaway-Mariano, D., Imker, H. J., Jacobson, M. P., Minor, W., Poulter, C. D., Raushel, F. M., Sali, A., Shoichet, B. K. & Sweedler, J. V., Nov 22 2011, In : Biochemistry. 50, 46, p. 9950-9962 13 p.

Research output: Contribution to journalReview article

Enzymes
Microbiology
Genes
Amidohydrolases
Genome
Vibrio cholerae
Cholera
Glycerol
Phospholipids
Fatty Acids
2012

Altered regulation of escherichia coli biotin biosynthesis in bira superrepressor mutant strains

Chakravartty, V. & Cronan, J. E., Mar 1 2012, In : Journal of bacteriology. 194, 5, p. 1113-1126 14 p.

Research output: Contribution to journalArticle

Biotin
Escherichia coli
Operon
Mutation
Ligases

Crosstalk of Escherichia coli FadR with Global Regulators in Expression of Fatty Acid Transport Genes

Feng, Y. & Cronan, J. E., Sep 28 2012, In : PloS one. 7, 9, e46275.

Research output: Contribution to journalArticle

Crosstalk
Escherichia coli
binding sites
Fatty Acids
Genes

Dimerization of the bacterial biotin carboxylase subunit is required for acetyl coenzyme A carboxylase activity In vivo

Smith, A. C. & Cronan, J. E., Jan 1 2012, In : Journal of bacteriology. 194, 1, p. 72-78 7 p.

Research output: Contribution to journalArticle

biotin carboxylase
Acetyl-CoA Carboxylase
Dimerization
Coenzyme A
Proteins

Evolution of a new function in an esterase: Simple amino acid substitutions enable the activity present in the larger paralog, BioH

Flores, H., Lin, S., Contreras-Ferrat, G., Cronan, J. E. & Morett, E., Aug 1 2012, In : Protein Engineering, Design and Selection. 25, 8, p. 387-395 9 p.

Research output: Contribution to journalArticle

Esterases
Amino Acid Substitution
Amino acids
Substitution reactions
Biotin

Only one of the five Ralstonia solanacearum long-chain 3-ketoacyl-acyl carrier protein synthase homologues functions in fatty acid synthesis

Cheng, J., Ma, J., Lin, J., Fan, Z. C., Cronan, J. E. & Wang, H., Mar 1 2012, In : Applied and environmental microbiology. 78, 5, p. 1563-1573 11 p.

Research output: Contribution to journalArticle

holo-(acyl-carrier-protein) synthase
Ralstonia solanacearum
acyl carrier protein
Fatty Acids
fatty acid

Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin

Shapiro, M. M., Chakravartty, V. & Cronan, J. E., Nov 9 2012, In : PloS one. 7, 11, e49440.

Research output: Contribution to journalArticle

biotin
Biotin
synthesis
Enzymes
enzymes
Acyl Carrier Protein
Biotin
Esters
Gatekeeping
Enzymes
Acyl Carrier Protein
Esterification
Methyltransferases
Biotin
Malonyl Coenzyme A

The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities

Bi, H., Christensen, Q. H., Feng, Y., Wang, H. & Cronan, J. E., Feb 1 2012, In : Molecular Microbiology. 83, 4, p. 840-855 16 p.

Research output: Contribution to journalArticle

Burkholderia cenocepacia
Enoyl-CoA Hydratase
Acyl Carrier Protein
Hydro-Lyases
Quorum Sensing
2013

Discovery of a cAMP deaminase that quenches cyclic AMP-dependent regulation

Goble, A. M., Feng, Y., Raushel, F. M. & Cronan, J. E., Dec 20 2013, In : ACS chemical biology. 8, 12, p. 2622-2629 8 p.

Research output: Contribution to journalArticle

Adenosine Deaminase
Adenosine Monophosphate
Cyclic AMP
Inosine Monophosphate
Genes

Discovery of new enzymes and metabolic pathways by using structure and genome context

Zhao, S., Kumar, R., Sakai, A., Vetting, M. W., Wood, B. M. K., Brown, S., Bonanno, J. B., Hillerich, B. S., Seidel, R. D., Babbitt, P. C., Almo, S. C., Sweedler, J. V., Gerlt, J. A., Cronan, J. E. & Jacobson, M. P., Sep 24 2013, In : Nature. 502, 7473, p. 698-702 5 p.

Research output: Contribution to journalArticle

Metabolic Networks and Pathways
Genome
Multigene Family
Substrate Specificity
Enzymes

FabQ, a dual-function dehydratase/isomerase, circumvents the last step of the classical fatty acid synthesis cycle

Bi, H., Wang, H. & Cronan, J. E., Sep 19 2013, In : Chemistry and Biology. 20, 9, p. 1157-1167 11 p.

Research output: Contribution to journalArticle

Hydro-Lyases
Isomerases
Unsaturated Fatty Acids
Fatty Acids
Escherichia coli
Poisons
Bacteriophages
Plasmids
Escherichia coli
Gene Expression

Profligate biotin synthesis in α-proteobacteria - a developing or degenerating regulatory system?

Feng, Y., Zhang, H. & Cronan, J. E., Apr 1 2013, In : Molecular Microbiology. 88, 1, p. 77-92 16 p.

Research output: Contribution to journalArticle

Proteobacteria
Biotin
Ligases
Bacteria
Proteins

Proofreading of noncognate Acyl adenylates by an acyl-coenzyme a ligase

Manandhar, M. & Cronan, J. E., Dec 19 2013, In : Chemistry and Biology. 20, 12, p. 1441-1446 6 p.

Research output: Contribution to journalArticle

Amino Acyl-tRNA Synthetases
Coenzymes
Ligases
Dicarboxylic Acids
Substrates

The role of the Saccharomyces cerevisiae lipoate protein ligase homologue, Lip3, in lipoic acid synthesis

Hermes, F. A. & Cronan, J. E., Oct 1 2013, In : Yeast. 30, 10, p. 415-427 13 p.

Research output: Contribution to journalArticle

Saccharomyces cerevisiae Proteins
Thioctic Acid
Yeast
Amino acids
Enzymes

The two functional enoyl-acyl carrier protein reductases of Enterococcus faecalis do not mediate triclosan resistance

Zhu, L., Bi, H., Ma, J., Hu, Z., Zhang, W., Cronan, J. E. & Wang, H., Oct 1 2013, In : mBio. 4, 5, e00613-13.

Research output: Contribution to journalArticle

Triclosan
Acyl Carrier Protein
Enterococcus faecalis
Oxidoreductases
Fatty Acids

The wing of a winged helix-turn-helix transcription factor organizes the active site of BirA, a bifunctional repressor/ligase

Chakravartty, V. & Cronan, J. E., Dec 13 2013, In : Journal of Biological Chemistry. 288, 50, p. 36029-36039 11 p.

Research output: Contribution to journalArticle

Ligases
Biotin
Catalytic Domain
Transcription Factors
Proteins
2014

A Francisella virulence factor catalyses an essential reaction of biotin synthesis

Feng, Y., Napier, B. A., Manandhar, M., Henke, S. K., Weiss, D. S. & Cronan, J. E., Jan 1 2014, In : Molecular Microbiology. 91, 2, p. 300-314 15 p.

Research output: Contribution to journalArticle

Francisella
Virulence Factors
Biotin
Virulence
Escherichia
Fatty Acid Synthesis Inhibitors
Fatty Acids
Bacteria
Anhydrides
Infection
Succinic Acid
Escherichia coli
Growth
Succinate Dehydrogenase
Ligases

Biotin and lipoic acid: Synthesis, attachment, and regulation

Cronan, J. E., Jan 1 2014, In : EcoSal Plus. 6, 1, p. 1-39 39 p.

Research output: Contribution to journalArticle

Thioctic Acid
Biotin
Coenzymes
Synthetic Genes
Proteins

Evidence against translational repression by the carboxyltransferase component of Escherichia coli acetyl coenzyme a carboxylase

Smith, A. C. & Cronan, J. E., Jan 1 2014, In : Journal of bacteriology. 196, 21, p. 3768-3775 8 p.

Research output: Contribution to journalArticle

Carboxyl and Carbamoyl Transferases
Coenzymes
Escherichia coli
Messenger RNA
Acetyl-CoA Carboxylase

Inefficient translation renders the enterococcus faecalis fabk enoyl- acyl carrier protein reductase phenotypically cryptic

Bi, H., Zhu, L., Wang, H. & Cronan, J. E., Jan 1 2014, In : Journal of bacteriology. 196, 1, p. 170-179 10 p.

Research output: Contribution to journalArticle

Acyl Carrier Protein
Enterococcus faecalis
Oxidoreductases
Growth
Mutation

PdhR, the pyruvate dehydrogenase repressor, does not regulate lipoic acid synthesis

Feng, Y. & Cronan, J. E., Jul 2014, In : Research in Microbiology. 165, 6, p. 429-438 10 p.

Research output: Contribution to journalArticle

Thioctic Acid
Pyruvic Acid
Oxidoreductases
Binding Sites
Galactosidases

Prediction and biochemical demonstration of a catabolic pathway for the osmoprotectant proline betaine

Kumar, R., Zhao, S., Vetting, M. W., Wood, B. M. K., Sakai, A., Cho, K., Solbiati, J., Almo, S. C., Sweedler, J. V., Jacobson, M. P., Gerlt, J. A. & Cronan, J. E., Feb 11 2014, In : mBio. 5, 1, e00933-13.

Research output: Contribution to journalArticle

Betaine
Paracoccus denitrificans
Metabolomics
Hydroxyproline
Rhodobacter sphaeroides

Roles of small laccases from streptomyces in lignin degradation

Majumdar, S., Lukk, T., Solbiati, J. O., Bauer, S., Nair, S. K., Cronan, J. E. & Gerlt, J. A., Jun 24 2014, In : Biochemistry. 53, 24, p. 4047-4058 12 p.

Research output: Contribution to journalArticle

Laccase
Lignin
Streptomyces
Degradation
Streptomyces coelicolor

Successful conversion of the Bacillus subtilis BirA group II biotin protein ligase into a group I ligase

Henke, S. K. & Cronan, J. E., May 9 2014, In : PloS one. 9, 5, e96757.

Research output: Contribution to journalArticle

Bacilli
Ligases
Biotin
Bacillus subtilis
ligases

The chain-flipping mechanism of ACP (acyl carrier protein)-dependent enzymes appears universal

Cronan, J. E., Jun 1 2014, In : Biochemical Journal. 460, 2, p. 157-163 7 p.

Research output: Contribution to journalReview article

Acyl Carrier Protein
Enzymes
Fatty Acids
Polyketides
Proteins
Biosynthetic Pathways
Sulfur
Thioctic Acid
Fatty Acid Synthases
Enzymes