7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708

P. B. Hylemon, A. F. Cacciapuoti, Bryan A White, T. R. Whitehead, R. J. Fricke

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Abstract

NADH:flavin oxidoreductase and 7α-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7α-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD + plus FADH 2. The pH optima of 7α-dehydroxylase activity in whole cells and cell extracts were 7.0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7α-dehydroxylation suggest a relationship between 7α-dehydroxylase and NADH:flavin oxidoreductase.

Original languageEnglish (US)
Pages (from-to)2507-2510
Number of pages4
JournalAmerican Journal of Clinical Nutrition
Volume33
Issue number11 SUPPL.
StatePublished - Dec 1 1980

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ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Hylemon, P. B., Cacciapuoti, A. F., White, B. A., Whitehead, T. R., & Fricke, R. J. (1980). 7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708. American Journal of Clinical Nutrition, 33(11 SUPPL.), 2507-2510.