7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708

P. B. Hylemon; A. F. Cacciapuoti; B. A. White; T. R. Whitehead; R. J. Fricke

doi:10.1093/ajcn/33.11.2507

7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708

P. B. Hylemon, A. F. Cacciapuoti, B. A. White, T. R. Whitehead, R. J. Fricke

Research output: Contribution to journal › Article › peer-review

Abstract

NADH:flavin oxidoreductase and 7α-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7α-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD⁺ plus FADH₂. The pH optima of 7α-dehydroxylase activity in whole cells and cell extracts were 7.0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7α-dehydroxylation suggest a relationship between 7α-dehydroxylase and NADH:flavin oxidoreductase.

Original language	English (US)
Pages (from-to)	2507-2510
Number of pages	4
Journal	American Journal of Clinical Nutrition
Volume	33
Issue number	11 SUPPL.
DOIs	https://doi.org/10.1093/ajcn/33.11.2507
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Medicine (miscellaneous)
Nutrition and Dietetics

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10.1093/ajcn/33.11.2507

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title = "7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708",

abstract = "NADH:flavin oxidoreductase and 7α-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7α-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD+ plus FADH2. The pH optima of 7α-dehydroxylase activity in whole cells and cell extracts were 7.0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7α-dehydroxylation suggest a relationship between 7α-dehydroxylase and NADH:flavin oxidoreductase.",

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T1 - 7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708

AU - Hylemon, P. B.

AU - Cacciapuoti, A. F.

AU - White, B. A.

AU - Whitehead, T. R.

AU - Fricke, R. J.

PY - 1980

Y1 - 1980

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AB - NADH:flavin oxidoreductase and 7α-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7α-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD+ plus FADH2. The pH optima of 7α-dehydroxylase activity in whole cells and cell extracts were 7.0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7α-dehydroxylation suggest a relationship between 7α-dehydroxylase and NADH:flavin oxidoreductase.

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7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708

Abstract

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