7α-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708

P. B. Hylemon, A. F. Cacciapuoti, B. A. White, T. R. Whitehead, R. J. Fricke

Research output: Contribution to journalArticlepeer-review

Abstract

NADH:flavin oxidoreductase and 7α-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7α-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD+ plus FADH2. The pH optima of 7α-dehydroxylase activity in whole cells and cell extracts were 7.0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7α-dehydroxylation suggest a relationship between 7α-dehydroxylase and NADH:flavin oxidoreductase.

Original languageEnglish (US)
Pages (from-to)2507-2510
Number of pages4
JournalUnknown Journal
Volume33
Issue number11 SUPPL.
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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