3D super-resolution imaging with blinking quantum dots

Yong Wang, Gilbert Fruhwirth, En Cai, Tony Ng, Paul R. Selvin

Research output: Contribution to journalArticlepeer-review

Abstract

Quantum dots are promising candidates for single molecule imaging due to their exceptional photophysical properties, including their intense brightness and resistance to photobleaching. They are also notorious for their blinking. Here we report a novel way to take advantage of quantum dot blinking to develop an imaging technique in three-dimensions with nanometric resolution. We first applied this method to simulated images of quantum dots and then to quantum dots immobilized on microspheres. We achieved imaging resolutions (fwhm) of 8-17 nm in the x-y plane and 58 nm (on coverslip) or 81 nm (deep in solution) in the z-direction, approximately 3-7 times better than what has been achieved previously with quantum dots. This approach was applied to resolve the 3D distribution of epidermal growth factor receptor (EGFR) molecules at, and inside of, the plasma membrane of resting basal breast cancer cells.

Original languageEnglish (US)
Pages (from-to)5233-5241
Number of pages9
JournalNano letters
Volume13
Issue number11
DOIs
StatePublished - Nov 13 2013

Keywords

  • 3D super-resolution
  • EGFR
  • blinking
  • gSHRImP
  • quantum dots

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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