TY - JOUR
T1 - 3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response
AU - Badea, Adina
AU - McCracken, Joselle M.
AU - Tillmaand, Emily G.
AU - Kandel, Mikhail E.
AU - Oraham, Aaron W.
AU - Mevis, Molly B.
AU - Rubakhin, Stanislav S.
AU - Popescu, Gabriel
AU - Sweedler, Jonathan V.
AU - Nuzzo, Ralph G.
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/13
Y1 - 2017/9/13
N2 - Understanding and controlling the interactions occurring between cells and engineered materials are central challenges toward progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-d-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system - organotypic primary murine dorsal root ganglion (DRG) - in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. This experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.
AB - Understanding and controlling the interactions occurring between cells and engineered materials are central challenges toward progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-d-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system - organotypic primary murine dorsal root ganglion (DRG) - in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. This experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.
KW - 3D cell culture
KW - 4D printing
KW - biologically compliant materials
KW - dorsal root ganglion (DRG)
KW - functional soft materials
KW - gels
KW - programmable cell-scaffold interactions
UR - http://www.scopus.com/inward/record.url?scp=85029444319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029444319&partnerID=8YFLogxK
U2 - 10.1021/acsami.7b06742
DO - 10.1021/acsami.7b06742
M3 - Article
C2 - 28813592
AN - SCOPUS:85029444319
SN - 1944-8244
VL - 9
SP - 30318
EP - 30328
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 36
ER -