TY - JOUR
T1 - 3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response
AU - Badea, Adina
AU - McCracken, Joselle M.
AU - Tillmaand, Emily G.
AU - Kandel, Mikhail E.
AU - Oraham, Aaron W.
AU - Mevis, Molly B.
AU - Rubakhin, Stanislav S.
AU - Popescu, Gabriel
AU - Sweedler, Jonathan V.
AU - Nuzzo, Ralph G.
N1 - Funding Information:
The authors would like to thank George A. Ibrahim for helping with cell tracking and SLIM data processing, Julio Soares (Laser and Spectroscopy Facility, Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana−Champaign) for assistance and advice regarding confocal fluorescence microscopy, and Professor Prashant Jain’s research group (Chemistry Department, University of Illinois at Urbana−Champaign) for use of their UV−vis spectrometer. The support of the materials development efforts in the work (J.M.M., R.G.N.) by the U.S. Department of Energy, Division of Materials Sciences under Award DE-FG02-07ER46471, through the Frederick Seitz Materials Research Laboratory at the University of Illinois at Urbana−Champaign, and of the functional printing methods (A.B., A.W.O., M.B.M.) by the Army Research Office (Award W911NF-13-0489), are gratefully acknowledged. The support of the National Institute on Drug Abuse through award P30 DA018310 facilitating studies of neuronal cultures (E.G.T., S.S.R., J.V.S.) is also gratefully acknowledged. M.E.K. and G.P. were supported by the National Science Foundation (NSF) Grants CBET-0939511 STC, DBI 14-50962 EAGER, and IIP-1353368.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/13
Y1 - 2017/9/13
N2 - Understanding and controlling the interactions occurring between cells and engineered materials are central challenges toward progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-d-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system - organotypic primary murine dorsal root ganglion (DRG) - in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. This experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.
AB - Understanding and controlling the interactions occurring between cells and engineered materials are central challenges toward progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-d-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system - organotypic primary murine dorsal root ganglion (DRG) - in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. This experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.
KW - 3D cell culture
KW - 4D printing
KW - biologically compliant materials
KW - dorsal root ganglion (DRG)
KW - functional soft materials
KW - gels
KW - programmable cell-scaffold interactions
UR - http://www.scopus.com/inward/record.url?scp=85029444319&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029444319&partnerID=8YFLogxK
U2 - 10.1021/acsami.7b06742
DO - 10.1021/acsami.7b06742
M3 - Article
C2 - 28813592
AN - SCOPUS:85029444319
SN - 1944-8244
VL - 9
SP - 30318
EP - 30328
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 36
ER -