Abstract
Materials chemistries for hydrogel scaffolds that are capable of programming temporal (4D) attributes of cellular decision-making in supported 3D microcultures are described. The scaffolds are fabricated using direct-ink writing (DIW)—a 3D-printing technique using extrusion to pattern scaffolds at biologically relevant diameters (≤ 100 µm). Herein, DIW is exploited to variously incorporate a rheological nanoclay, Laponite XLG (LAP), into 2-hydroxyethyl methacrylate (HEMA)-based hydrogels—printing the LAP–HEMA (LH) composites as functional modifiers within otherwise unmodified 2D and 3D HEMA microstructures. The nanoclay-modified domains, when tested as thin films, require no activating (e.g., protein) treatments to promote robust growth compliances that direct the spatial attachment of fibroblast (3T3) and preosteoblast (E1) cells, fostering for the latter a capacity to direct long-term osteodifferentiation. Cell-to-gel interfacial morphologies and cellular motility are analyzed with spatial light interference microscopy (SLIM). Through combination of HEMA and LH gels, high-resolution DIW of a nanocomposite ink (UniH) that translates organizationally dynamic attributes seen with 2D gels into dentition-mimetic 3D scaffolds is demonstrated. These analyses confirm that the underlying materials chemistry and geometry of hydrogel nanocomposites are capable of directing cellular attachment and temporal development within 3D microcultures—a useful material system for the 4D patterning of hydrogel scaffolds.
| Original language | English (US) |
|---|---|
| Article number | 1800788 |
| Journal | Advanced Healthcare Materials |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 10 2019 |
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Keywords
- 3D printing
- biomineralization
- cellular biocompliance
- direct ink write 4D printing
- hydrogel nanocomposites
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- Pharmaceutical Science
Cite this
3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization. / McCracken, Joselle M.; Rauzan, Brittany M.; Kjellman, Jacob C.E.; Kandel, Mikhail E.; Liu, Yu Hao; Badea, Adina; Miller, Lou Ann; Rogers, Simon A.; Popescu, Gabriel; Nuzzo, Ralph G.
In: Advanced Healthcare Materials, Vol. 8, No. 1, 1800788, 10.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization
AU - McCracken, Joselle M.
AU - Rauzan, Brittany M.
AU - Kjellman, Jacob C.E.
AU - Kandel, Mikhail E.
AU - Liu, Yu Hao
AU - Badea, Adina
AU - Miller, Lou Ann
AU - Rogers, Simon A.
AU - Popescu, Gabriel
AU - Nuzzo, Ralph G.
PY - 2019/1/10
Y1 - 2019/1/10
N2 - Materials chemistries for hydrogel scaffolds that are capable of programming temporal (4D) attributes of cellular decision-making in supported 3D microcultures are described. The scaffolds are fabricated using direct-ink writing (DIW)—a 3D-printing technique using extrusion to pattern scaffolds at biologically relevant diameters (≤ 100 µm). Herein, DIW is exploited to variously incorporate a rheological nanoclay, Laponite XLG (LAP), into 2-hydroxyethyl methacrylate (HEMA)-based hydrogels—printing the LAP–HEMA (LH) composites as functional modifiers within otherwise unmodified 2D and 3D HEMA microstructures. The nanoclay-modified domains, when tested as thin films, require no activating (e.g., protein) treatments to promote robust growth compliances that direct the spatial attachment of fibroblast (3T3) and preosteoblast (E1) cells, fostering for the latter a capacity to direct long-term osteodifferentiation. Cell-to-gel interfacial morphologies and cellular motility are analyzed with spatial light interference microscopy (SLIM). Through combination of HEMA and LH gels, high-resolution DIW of a nanocomposite ink (UniH) that translates organizationally dynamic attributes seen with 2D gels into dentition-mimetic 3D scaffolds is demonstrated. These analyses confirm that the underlying materials chemistry and geometry of hydrogel nanocomposites are capable of directing cellular attachment and temporal development within 3D microcultures—a useful material system for the 4D patterning of hydrogel scaffolds.
AB - Materials chemistries for hydrogel scaffolds that are capable of programming temporal (4D) attributes of cellular decision-making in supported 3D microcultures are described. The scaffolds are fabricated using direct-ink writing (DIW)—a 3D-printing technique using extrusion to pattern scaffolds at biologically relevant diameters (≤ 100 µm). Herein, DIW is exploited to variously incorporate a rheological nanoclay, Laponite XLG (LAP), into 2-hydroxyethyl methacrylate (HEMA)-based hydrogels—printing the LAP–HEMA (LH) composites as functional modifiers within otherwise unmodified 2D and 3D HEMA microstructures. The nanoclay-modified domains, when tested as thin films, require no activating (e.g., protein) treatments to promote robust growth compliances that direct the spatial attachment of fibroblast (3T3) and preosteoblast (E1) cells, fostering for the latter a capacity to direct long-term osteodifferentiation. Cell-to-gel interfacial morphologies and cellular motility are analyzed with spatial light interference microscopy (SLIM). Through combination of HEMA and LH gels, high-resolution DIW of a nanocomposite ink (UniH) that translates organizationally dynamic attributes seen with 2D gels into dentition-mimetic 3D scaffolds is demonstrated. These analyses confirm that the underlying materials chemistry and geometry of hydrogel nanocomposites are capable of directing cellular attachment and temporal development within 3D microcultures—a useful material system for the 4D patterning of hydrogel scaffolds.
KW - 3D printing
KW - biomineralization
KW - cellular biocompliance
KW - direct ink write 4D printing
KW - hydrogel nanocomposites
UR - http://www.scopus.com/inward/record.url?scp=85058860218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85058860218&partnerID=8YFLogxK
U2 - 10.1002/adhm.201800788
DO - 10.1002/adhm.201800788
M3 - Article
C2 - 30565889
AN - SCOPUS:85058860218
VL - 8
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 1
M1 - 1800788
ER -