Abstract

Three-dimensional (3D) microperiodic scaffolds of poly(2-hydroxyethyl methacrylate) (pHEMA) have been fabricated by direct-write assembly of a photopolymerizable hydrogel ink. The ink is initially composed of physically entangled pHEMA chains dissolved in a solution of HEMA monomer, comonomer, photoinitiator and water. Upon printing 3D scaffolds of varying architecture, the ink filaments are exposed to UV light, where they are transformed into an interpenetrating hydrogel network of chemically cross-linked and physically entangled pHEMA chains. These 3D microperiodic scaffolds are rendered growth compliant for primary rat hippocampal neurons by absorption of polylysine. Neuronal cells thrive on these scaffolds, forming differentiated, intricately branched networks. Confocal laser scanning microscopy reveals that both cell distribution and extent of neuronal process alignment depend upon scaffold architecture. This work provides an important step forward in the creation of suitable platforms for in vitro study of sensitive cell types. 3D microperiodic hydrogel scaffolds with varied architecture are patterned by direct-write assembly and investigated as culture platforms for primary hippocampal neurons. Neuronal cells thrive on these scaffolds, forming differentiated, intricately branched networks. Confocal laser scanning microscopy reveals that both cell distribution and extent of neuronal process alignment depend upon scaffold architecture.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalAdvanced Functional Materials
Volume21
Issue number1
DOIs
StatePublished - Jan 7 2011

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Hydrogel
Hydrogels
Scaffolds
inks
cells
neurons
platforms
assembly
alignment
Ink
microscopy
scanning
printing
rats
Neurons
lasers
filaments
Microscopic examination
monomers
Scanning

Keywords

  • 3D Cell Culture
  • Direct-Write Assembly
  • Hippocampal Neurons
  • Tissue Engineering

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

3D microperiodic hydrogel scaffolds for robust neuronal cultures. / Hanson Shepherd, Jennifer N.; Parker, Sara T.; Shepherd, Robert F.; Gillette, Martha L; Lewis, Jennifer A.; Nuzzo, Ralph G.

In: Advanced Functional Materials, Vol. 21, No. 1, 07.01.2011, p. 47-54.

Research output: Contribution to journalArticle

Hanson Shepherd, Jennifer N. ; Parker, Sara T. ; Shepherd, Robert F. ; Gillette, Martha L ; Lewis, Jennifer A. ; Nuzzo, Ralph G. / 3D microperiodic hydrogel scaffolds for robust neuronal cultures. In: Advanced Functional Materials. 2011 ; Vol. 21, No. 1. pp. 47-54.
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