3D laser optoacoustic ultrasonic imaging system for research in mice (LOUIS-3DM)

Sergey A. Ermilov, Richard Su, Andre Conjusteau, Vassili Ivanov, Vyacheslav Nadvoretskiy, Tanmayi Oruganti, Pratik Talole, Fatima Anis, Mark A. Anastasio, Alexander A. Oraevsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution


In this work we introduce an improved prototype of three-dimensional imaging system that combines optoacoustic tomography (OAT) and laser ultrasound tomography (LUT) to obtain coregistered maps of tissue optical absorption and speed of sound (SoS). The OAT scan is performed by a 360 degree rotation of a mouse with respect to an arc-shaped array of ultrasonic transducers. A Q-switched laser system is used to establish optoacoustic illumination pattern appropriate for deep tissue imaging with a tunable (730-840 nm) output wavelengths operated at 10 Hz pulse repetition rate. A 532 nm wavelength output, being mostly absorbed within a narrow superficial layer of skin, is used to outline the visualized biological object. Broadband laser ultrasound emitters are arranged in another arc pattern and are positioned opposite and orthogonal to the array of transducers. This imaging geometry allows reconstruction of volumes that depict SoS distributions from the measured time of flight data. The reconstructed LUT images can subsequently be employed by an optoacoustic reconstruction algorithm to compensate for acoustic wavefield aberration and thereby improve accuracy of the reconstructed images of the absorbed optical energy. The coregistered OAT-LUT imaging is validated in a phantom and live mouse using a single-slice system prototype.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2014
ISBN (Print)9780819498564
StatePublished - Jan 1 2014
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2014 - San Francisco, CA, United States
Duration: Feb 2 2014Feb 5 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotons Plus Ultrasound: Imaging and Sensing 2014
Country/TerritoryUnited States
CitySan Francisco, CA


  • Laser ultrasound
  • Optical absorption
  • Photoacoustic tomography
  • Speed of sound tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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