3.5 Quantifying integrin-ligand engagement and cell phenotype in 3d scaffolds

R. J. DeVolder, H. Kong

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The use of cells in clinical therapies holds great promise for the treatment of a wide range of diseases and disorders. However, the fate of cells depends on several soluble and insoluble environmental factors. It is well known that bonds formed between cells and their surrounding environments can direct cellular activities, phenotypes, etc. Therefore, extensive efforts have been made to develop methods to characterize the engagement of integrins with adhesion ligands and their effects on cell adhesion, signaling activation, and phenotypic activities, both in vitro and in vivo. This chapter summarizes methods developed to quantitatively evaluate (1) cell adhesion forces and (2) the number of integrin-ligand bonds formed on two-dimensional (2D) and within 3D platforms, all of which can be examined with respect to cellular phenotypic properties and cellular fates.

Original languageEnglish (US)
Title of host publicationComprehensive Biomaterials II
PublisherElsevier
Pages78-85
Number of pages8
ISBN (Electronic)9780081006924
ISBN (Print)9780081006917
DOIs
StatePublished - Jan 1 2017

Keywords

  • Atomic force microscopy
  • Extracellular matrix
  • Fluorescence resonance energy transfer
  • Hydrogel
  • Immunofluorescence
  • Integrin
  • Ligand
  • Magnetic twisting cytometry
  • RGD peptide
  • Traction force microscopy

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)

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