2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer

Christoph Nowak, Thamara Laredo, Jens Gebert, Jacek Lipkowski, Robert B Gennis, Shelagh Ferguson-Miller, Wolfgang Knoll, Renate L C Naumann

Research output: Contribution to journalArticle

Abstract

Potentiometric titrations of the cytochrome c oxidase (CcO) immobilized in a biomimetic membrane system were followed by two-dimensional surface-enhanced IR absorption spectroscopy (2D SEIRAS) in the ATR-mode. Direct electron transfer was employed to vary the redox state of the enzyme. The CcO was shown to undergo a conformational transition from a non-activated to an activated state after it was allowed to turnover in the presence of oxygen. Differences between the non-activated and activated state were revealed by 2D SEIRA spectra recorded as a function of potential. The activated state was characterized by a higher number of correlated transitions as well as a higher number of amino acids associated with electron transfer.

Original languageEnglish (US)
Pages (from-to)619-627
Number of pages9
JournalMetallomics
Volume3
Issue number6
DOIs
StatePublished - Jun 1 2011

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Electron Transport Complex IV
Spectrum Analysis
Spectroscopy
Electrons
Biomimetics
Titration
Absorption spectroscopy
Oxidation-Reduction
Amino acids
Infrared spectroscopy
Enzymes
Oxygen
Membranes
Amino Acids

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics

Cite this

2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer. / Nowak, Christoph; Laredo, Thamara; Gebert, Jens; Lipkowski, Jacek; Gennis, Robert B; Ferguson-Miller, Shelagh; Knoll, Wolfgang; Naumann, Renate L C.

In: Metallomics, Vol. 3, No. 6, 01.06.2011, p. 619-627.

Research output: Contribution to journalArticle

Nowak, C, Laredo, T, Gebert, J, Lipkowski, J, Gennis, RB, Ferguson-Miller, S, Knoll, W & Naumann, RLC 2011, '2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer', Metallomics, vol. 3, no. 6, pp. 619-627. https://doi.org/10.1039/c0mt00083c
Nowak, Christoph ; Laredo, Thamara ; Gebert, Jens ; Lipkowski, Jacek ; Gennis, Robert B ; Ferguson-Miller, Shelagh ; Knoll, Wolfgang ; Naumann, Renate L C. / 2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer. In: Metallomics. 2011 ; Vol. 3, No. 6. pp. 619-627.
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