2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer

Christoph Nowak; Thamara Laredo; Jens Gebert; Jacek Lipkowski; Robert B Gennis; Shelagh Ferguson-Miller; Wolfgang Knoll; Renate L C Naumann

doi:10.1039/c0mt00083c

2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer

Christoph Nowak, Thamara Laredo, Jens Gebert, Jacek Lipkowski, Robert B Gennis, Shelagh Ferguson-Miller, Wolfgang Knoll, Renate L C Naumann

Biochemistry

Research output: Contribution to journal › Article

Abstract

Potentiometric titrations of the cytochrome c oxidase (CcO) immobilized in a biomimetic membrane system were followed by two-dimensional surface-enhanced IR absorption spectroscopy (2D SEIRAS) in the ATR-mode. Direct electron transfer was employed to vary the redox state of the enzyme. The CcO was shown to undergo a conformational transition from a non-activated to an activated state after it was allowed to turnover in the presence of oxygen. Differences between the non-activated and activated state were revealed by 2D SEIRA spectra recorded as a function of potential. The activated state was characterized by a higher number of correlated transitions as well as a higher number of amino acids associated with electron transfer.

Original language	English (US)
Pages (from-to)	619-627
Number of pages	9
Journal	Metallomics
Volume	3
Issue number	6
DOIs	https://doi.org/10.1039/c0mt00083c
State	Published - Jun 1 2011

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ASJC Scopus subject areas

Biomaterials
Metals and Alloys
Chemistry (miscellaneous)
Biochemistry
Biophysics

Cite this

Nowak, C., Laredo, T., Gebert, J., Lipkowski, J., Gennis, R. B., Ferguson-Miller, S., Knoll, W., & Naumann, R. L. C. (2011). 2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer. Metallomics, 3(6), 619-627. https://doi.org/10.1039/c0mt00083c

2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer. / Nowak, Christoph; Laredo, Thamara; Gebert, Jens; Lipkowski, Jacek; Gennis, Robert B; Ferguson-Miller, Shelagh; Knoll, Wolfgang; Naumann, Renate L C.

In: Metallomics, Vol. 3, No. 6, 01.06.2011, p. 619-627.

Research output: Contribution to journal › Article

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10.1039/c0mt00083c