2D-SEIRA spectroscopy to highlight conformational changes of the cytochrome c oxidase induced by direct electron transfer

Christoph Nowak, Thamara Laredo, Jens Gebert, Jacek Lipkowski, Robert B. Gennis, Shelagh Ferguson-Miller, Wolfgang Knoll, Renate L.C. Naumann

Research output: Contribution to journalArticlepeer-review

Abstract

Potentiometric titrations of the cytochrome c oxidase (CcO) immobilized in a biomimetic membrane system were followed by two-dimensional surface-enhanced IR absorption spectroscopy (2D SEIRAS) in the ATR-mode. Direct electron transfer was employed to vary the redox state of the enzyme. The CcO was shown to undergo a conformational transition from a non-activated to an activated state after it was allowed to turnover in the presence of oxygen. Differences between the non-activated and activated state were revealed by 2D SEIRA spectra recorded as a function of potential. The activated state was characterized by a higher number of correlated transitions as well as a higher number of amino acids associated with electron transfer.

Original languageEnglish (US)
Pages (from-to)619-627
Number of pages9
JournalMetallomics
Volume3
Issue number6
DOIs
StatePublished - Jun 2011

ASJC Scopus subject areas

  • General Medicine

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