2-Keto-4-(methylthio)butyric acid (keto analog of methionine) is a safe and efficacious precursor of L-methionine in chicks

Ryan Neil Dilger, Christoph Kobler, Christoph Weckbecker, Dirk Hoehler, David H. Baker

Research output: Contribution to journalArticle

Abstract

Relative bioefficacy and toxicity of Met precursor compounds were investigated in young chicks. The effectiveness of DL-Met and 2-keto-4-(methylthio)butyric acid (Keto-Met) to serve as L-Met precursors was quantified using Met-deficient diets of differing composition. Efficacy was based on slope-ratio and standard-curve methodology. Using L-Met as a standard Met source added to a purified diet, DL-Met and Keto-Met were assigned relative bioefficacy values of 98.5 and 92.5%, respectively, based on weight gain. Relative bioefficacy values of 98.5 and 89.3% were assigned to DL-Met and Keto-Met, respectively, when chicks were fed a Met-deficient, corn-soybean meal-peanut meal diet. Thus, both DL-Met and Keto-Met are effective Met precursor compounds in chicks. Additionally, growth-depressing effects of L-Met, DL-Met, and Keto-Met were compared using a nutritionally adequate corn-soybean meal diet supplemented with 15 or 30 g/kg of each compound. Similar reductions in weight gain, food intake, and gain:food ratio were observed for each compound. Subjective spleen color scores, indicative of splenic hemosiderosis, increased linearly (P < 0.01) with increasing intakes of each compound, suggesting a similarity in overall toxicity among these compounds. Because conversion of Keto-Met to L-Met in vivo merely requires transamination, Keto-Met may prove to be a useful supplement not only in food animal production, but also as a component of enteral and parenteral formulas for humans suffering from renal insufficiency.

Original languageEnglish (US)
Pages (from-to)1868-1873
Number of pages6
JournalJournal of Nutrition
Volume137
Issue number8
StatePublished - Aug 1 2007

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Butyric Acid
Methionine
Diet
Meals
Soybeans
Zea mays
Weight Gain
Hemosiderosis
Food
Small Intestine
Renal Insufficiency
Spleen
Color
Eating
Growth

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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2-Keto-4-(methylthio)butyric acid (keto analog of methionine) is a safe and efficacious precursor of L-methionine in chicks. / Dilger, Ryan Neil; Kobler, Christoph; Weckbecker, Christoph; Hoehler, Dirk; Baker, David H.

In: Journal of Nutrition, Vol. 137, No. 8, 01.08.2007, p. 1868-1873.

Research output: Contribution to journalArticle

Dilger, Ryan Neil ; Kobler, Christoph ; Weckbecker, Christoph ; Hoehler, Dirk ; Baker, David H. / 2-Keto-4-(methylthio)butyric acid (keto analog of methionine) is a safe and efficacious precursor of L-methionine in chicks. In: Journal of Nutrition. 2007 ; Vol. 137, No. 8. pp. 1868-1873.
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abstract = "Relative bioefficacy and toxicity of Met precursor compounds were investigated in young chicks. The effectiveness of DL-Met and 2-keto-4-(methylthio)butyric acid (Keto-Met) to serve as L-Met precursors was quantified using Met-deficient diets of differing composition. Efficacy was based on slope-ratio and standard-curve methodology. Using L-Met as a standard Met source added to a purified diet, DL-Met and Keto-Met were assigned relative bioefficacy values of 98.5 and 92.5{\%}, respectively, based on weight gain. Relative bioefficacy values of 98.5 and 89.3{\%} were assigned to DL-Met and Keto-Met, respectively, when chicks were fed a Met-deficient, corn-soybean meal-peanut meal diet. Thus, both DL-Met and Keto-Met are effective Met precursor compounds in chicks. Additionally, growth-depressing effects of L-Met, DL-Met, and Keto-Met were compared using a nutritionally adequate corn-soybean meal diet supplemented with 15 or 30 g/kg of each compound. Similar reductions in weight gain, food intake, and gain:food ratio were observed for each compound. Subjective spleen color scores, indicative of splenic hemosiderosis, increased linearly (P < 0.01) with increasing intakes of each compound, suggesting a similarity in overall toxicity among these compounds. Because conversion of Keto-Met to L-Met in vivo merely requires transamination, Keto-Met may prove to be a useful supplement not only in food animal production, but also as a component of enteral and parenteral formulas for humans suffering from renal insufficiency.",
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