2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2

Jacob Gyore, Archana R. Parameswar, Carleigh F.F. Hebbard, Younghoon Oh, Erfei Bi, Alexei V. Demchenko, Neil P. Price, Peter Orlean

Research output: Contribution to journalArticle

Abstract

Background: Chitin synthases are stimulated by N-acetylglucosamine (GlcNAc). Results: GlcNAc and 2-acylamido analogues of GlcNAc stimulate formation of chitin oligosaccharides by yeast chitin synthase, and GlcNAc is transferred to the 2-acylamido analogues. Conclusion: Chitin synthases use GlcNAc analogues as primers and transfer one GlcNAc at a time. Significance: Results are new insights into polysaccharide synthase mechanism and suggest ways of synthesizing novel modified polysaccharides.

Original languageEnglish (US)
Pages (from-to)12835-12841
Number of pages7
JournalJournal of Biological Chemistry
Volume289
Issue number18
DOIs
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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