TY - JOUR
T1 - 2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2
AU - Gyore, Jacob
AU - Parameswar, Archana R.
AU - Hebbard, Carleigh F.F.
AU - Oh, Younghoon
AU - Bi, Erfei
AU - Demchenko, Alexei V.
AU - Price, Neil P.
AU - Orlean, Peter
PY - 2014
Y1 - 2014
N2 - Background: Chitin synthases are stimulated by N-acetylglucosamine (GlcNAc). Results: GlcNAc and 2-acylamido analogues of GlcNAc stimulate formation of chitin oligosaccharides by yeast chitin synthase, and GlcNAc is transferred to the 2-acylamido analogues. Conclusion: Chitin synthases use GlcNAc analogues as primers and transfer one GlcNAc at a time. Significance: Results are new insights into polysaccharide synthase mechanism and suggest ways of synthesizing novel modified polysaccharides.
AB - Background: Chitin synthases are stimulated by N-acetylglucosamine (GlcNAc). Results: GlcNAc and 2-acylamido analogues of GlcNAc stimulate formation of chitin oligosaccharides by yeast chitin synthase, and GlcNAc is transferred to the 2-acylamido analogues. Conclusion: Chitin synthases use GlcNAc analogues as primers and transfer one GlcNAc at a time. Significance: Results are new insights into polysaccharide synthase mechanism and suggest ways of synthesizing novel modified polysaccharides.
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U2 - 10.1074/jbc.M114.550749
DO - 10.1074/jbc.M114.550749
M3 - Article
C2 - 24619411
AN - SCOPUS:84899718033
SN - 0021-9258
VL - 289
SP - 12835
EP - 12841
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 18
ER -