2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2

Jacob Gyore, Archana R. Parameswar, Carleigh F.F. Hebbard, Younghoon Oh, Erfei Bi, Alexei V. Demchenko, Neil P. Price, Peter Orlean

Research output: Contribution to journalArticle

Abstract

Background: Chitin synthases are stimulated by N-acetylglucosamine (GlcNAc). Results: GlcNAc and 2-acylamido analogues of GlcNAc stimulate formation of chitin oligosaccharides by yeast chitin synthase, and GlcNAc is transferred to the 2-acylamido analogues. Conclusion: Chitin synthases use GlcNAc analogues as primers and transfer one GlcNAc at a time. Significance: Results are new insights into polysaccharide synthase mechanism and suggest ways of synthesizing novel modified polysaccharides.

Original languageEnglish (US)
Pages (from-to)12835-12841
Number of pages7
JournalJournal of Biological Chemistry
Volume289
Issue number18
DOIs
StatePublished - Jan 1 2014

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Chitin Synthase
Chitin
Acetylglucosamine
Oligosaccharides
Yeast
Yeasts
Polysaccharides
chitin synthase 2

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2. / Gyore, Jacob; Parameswar, Archana R.; Hebbard, Carleigh F.F.; Oh, Younghoon; Bi, Erfei; Demchenko, Alexei V.; Price, Neil P.; Orlean, Peter.

In: Journal of Biological Chemistry, Vol. 289, No. 18, 01.01.2014, p. 12835-12841.

Research output: Contribution to journalArticle

Gyore, Jacob ; Parameswar, Archana R. ; Hebbard, Carleigh F.F. ; Oh, Younghoon ; Bi, Erfei ; Demchenko, Alexei V. ; Price, Neil P. ; Orlean, Peter. / 2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 18. pp. 12835-12841.
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AU - Oh, Younghoon

AU - Bi, Erfei

AU - Demchenko, Alexei V.

AU - Price, Neil P.

AU - Orlean, Peter

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