[18] Nuclear Magnetic Resonance Studies of Biological and Model Membrane Systems

D. Chapman, E. Oldfield

Research output: Contribution to journalArticlepeer-review

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a technique in which nulcei with spin I ≥ 1/2 absorb radiofrequency energy of a particular frequency, in a magnetic field. For a given external magnetic field strength, different nuclei absorb energy at different frequencies. Thus it is possible to study, for example, carbon-13 or phosphorus-31 nuclei in a complex biochemical system, all other nuclei in the system remaining “transparent.” The shape and width of an NMR absorption line, and the magnitude of its relaxation times, can all give interesting information on the mobility of the group containing the nucleus under observation, and this can lead to a better understanding of molecular mobility in complex systems.

Original languageEnglish (US)
Pages (from-to)198-211
Number of pages14
JournalMethods in enzymology
Volume32
Issue numberC
DOIs
StatePublished - Jan 1 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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