Nuclear magnetic resonance (NMR) spectroscopy is a technique in which nulcei with spin I ≥ 1/2 absorb radiofrequency energy of a particular frequency, in a magnetic field. For a given external magnetic field strength, different nuclei absorb energy at different frequencies. Thus it is possible to study, for example, carbon-13 or phosphorus-31 nuclei in a complex biochemical system, all other nuclei in the system remaining “transparent.” The shape and width of an NMR absorption line, and the magnitude of its relaxation times, can all give interesting information on the mobility of the group containing the nucleus under observation, and this can lead to a better understanding of molecular mobility in complex systems.
ASJC Scopus subject areas
- Molecular Biology