17O NMR Spectral Properties of Pyrophosphate, Simple Phosphonates, and Thiophosphate and Phosphonate Analogues of ATP

John A. Gerlt, Mark A. Reynolds, Peter C. Demou, George L. Kenyon

Research output: Contribution to journalArticle

Abstract

The chemical shifts of the 170 resonances associated with the bridging and nonbridging oxygens of pyrophosphate have been unambiguously assigned; this information allows the assignment of the resonances of all of the phosphoryl oxygens of ADP and ATP. The chemical shifts of the phosphoryl oxygens in pyrophosphate and several nucleotide analogues with predictable proton ionization behavior, i.e., AMPS, ATPyS, methylphosphonate, methylenediphosphonate, and the ²,y-methylene analogue of ATP, have been measured as a function of pH; in each case an upfield change in chemical shift is observed upon protonation, with the magnitude of the shift change being approximately 50 ppm. These observations and those previously described (Gerlt, J. A.; Demou, P. C; Mehdi, S. J. Am. Chem. Soc. 1982, 104, 2848) demonstrate that 170 NMR spectroscopy can be used to quantitate the site and degree of charge neutralization in phosphate ester anions and related species.

Original languageEnglish (US)
Pages (from-to)6469-6475
Number of pages7
JournalJournal of the American Chemical Society
Volume105
Issue number21
DOIs
StatePublished - Oct 1983

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Organophosphonates
Adenosinetriphosphate
Chemical shift
nuclear magnetic resonance
Adenosine Triphosphate
Nuclear magnetic resonance
Oxygen
oxygen
Administrative data processing
Protonation
neutralization
Nucleotides
Adenosine Diphosphate
Nuclear magnetic resonance spectroscopy
ester
Ionization
Anions
Protons
anion
Esters

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

17O NMR Spectral Properties of Pyrophosphate, Simple Phosphonates, and Thiophosphate and Phosphonate Analogues of ATP. / Gerlt, John A.; Reynolds, Mark A.; Demou, Peter C.; Kenyon, George L.

In: Journal of the American Chemical Society, Vol. 105, No. 21, 10.1983, p. 6469-6475.

Research output: Contribution to journalArticle

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