17β-estradiol modulates gene expression in the female mouse cerebral cortex

Gwendolyn I. Humphreys, Yvonne S. Ziegler, Ann M. Nardulli

Research output: Contribution to journalArticle

Abstract

17β-estradiol (E2) plays critical roles in a number of target tissues including the mammary gland, reproductive tract, bone, and brain. Although it is clear that E2 reduces inflammation and ischemia-induced damage in the cerebral cortex, the molecular mechanisms mediating the effects of E2 in this brain region are lacking. Thus, we examined the cortical transcriptome using a mouse model system. Female adult mice were ovariectomized and implanted with silastic tubing containing oil or E2. After 7 days, the cerebral cortices were dissected and RNA was isolated and analyzed using RNA-sequencing. Analysis of the transcriptomes of control and E2-treated animals revealed that E2 treatment significantly altered the transcript levels of 88 genes. These genes were associated with long term synaptic potentiation, myelination, phosphoprotein phosphatase activity, mitogen activated protein kinase, and phosphatidylinositol 3-kinase signaling. E2 also altered the expression of genes linked to lipid synthesis and metabolism, vasoconstriction and vasodilation, cell-cell communication, and histone modification. These results demonstrate the far-reaching and diverse effects of E2 in the cerebral cortex and provide valuable insight to begin to understand cortical processes that may fluctuate in a dynamic hormonal environment.

Original languageEnglish (US)
Article numbere111975
JournalPloS one
Volume9
Issue number11
DOIs
StatePublished - Nov 5 2014

Fingerprint

cerebral cortex
Gene expression
Cerebral Cortex
estradiol
Estradiol
Genes
Gene Expression
gene expression
Brain
mice
Histone Code
Phosphatidylinositol 3-Kinase
RNA
myelination
RNA Sequence Analysis
brain
phosphoprotein phosphatase
cell communication
vasoconstriction
vasodilation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

17β-estradiol modulates gene expression in the female mouse cerebral cortex. / Humphreys, Gwendolyn I.; Ziegler, Yvonne S.; Nardulli, Ann M.

In: PloS one, Vol. 9, No. 11, e111975, 05.11.2014.

Research output: Contribution to journalArticle

Humphreys, Gwendolyn I. ; Ziegler, Yvonne S. ; Nardulli, Ann M. / 17β-estradiol modulates gene expression in the female mouse cerebral cortex. In: PloS one. 2014 ; Vol. 9, No. 11.
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