[13] Purification of the aa3-Type Cytochrome-c Oxidase from Rhodopseudomonas Sphaeroides

Angelo Azzi, Robert B. Gennis

Research output: Contribution to journalArticle

Abstract

This chapter describes the purification of the aa3-type cytochrome-c oxidase from Rhodopseudomonas sphaeroides. The chapter outlines the procedures for the preparation of the bacterial membranes and purification of the oxidase. R. sphaeroides is a nonsulfur bacterium that, when grown anaerobically in the light, develops a photosynthetic apparatus. In the dark and in the presence of oxygen, the photosynthetic apparatus is suppressed and the bacterium utilizes a respiratory chain. The cytochrome-c oxidase from R. sphaeroides can easily and quickly be prepared in small amounts. The enzyme is immunologically related to that found both in yeast mitochondria and P. denitrificans. This oxidase appears to be structurally similar to other bacterial aa3-type oxidases, though does not function as a proton pump. The success of the purification of the oxidase critically depends on the purity of the cytoplasmic membrane preparation.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalMethods in enzymology
Volume126
Issue numberC
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of '[13] Purification of the aa<sub>3</sub>-Type Cytochrome-c Oxidase from Rhodopseudomonas Sphaeroides'. Together they form a unique fingerprint.

  • Cite this