[13] Purification of the aa3-Type Cytochrome-c Oxidase from Rhodopseudomonas Sphaeroides

Angelo Azzi, Robert B Gennis

Research output: Contribution to journalArticle

Abstract

This chapter describes the purification of the aa3-type cytochrome-c oxidase from Rhodopseudomonas sphaeroides. The chapter outlines the procedures for the preparation of the bacterial membranes and purification of the oxidase. R. sphaeroides is a nonsulfur bacterium that, when grown anaerobically in the light, develops a photosynthetic apparatus. In the dark and in the presence of oxygen, the photosynthetic apparatus is suppressed and the bacterium utilizes a respiratory chain. The cytochrome-c oxidase from R. sphaeroides can easily and quickly be prepared in small amounts. The enzyme is immunologically related to that found both in yeast mitochondria and P. denitrificans. This oxidase appears to be structurally similar to other bacterial aa3-type oxidases, though does not function as a proton pump. The success of the purification of the oxidase critically depends on the purity of the cytoplasmic membrane preparation.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalMethods in enzymology
Volume126
Issue numberC
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Rhodobacter sphaeroides
Electron Transport Complex IV
Purification
Oxidoreductases
Bacteria
Membranes
Proton Pumps
Mitochondria
Electron Transport
Yeast
Yeasts
Cell Membrane
Oxygen
Light
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

[13] Purification of the aa3-Type Cytochrome-c Oxidase from Rhodopseudomonas Sphaeroides. / Azzi, Angelo; Gennis, Robert B.

In: Methods in enzymology, Vol. 126, No. C, 01.01.1986, p. 138-145.

Research output: Contribution to journalArticle

@article{632d3b19b0dc4561810d7c2a4484a24d,
title = "[13] Purification of the aa3-Type Cytochrome-c Oxidase from Rhodopseudomonas Sphaeroides",
abstract = "This chapter describes the purification of the aa3-type cytochrome-c oxidase from Rhodopseudomonas sphaeroides. The chapter outlines the procedures for the preparation of the bacterial membranes and purification of the oxidase. R. sphaeroides is a nonsulfur bacterium that, when grown anaerobically in the light, develops a photosynthetic apparatus. In the dark and in the presence of oxygen, the photosynthetic apparatus is suppressed and the bacterium utilizes a respiratory chain. The cytochrome-c oxidase from R. sphaeroides can easily and quickly be prepared in small amounts. The enzyme is immunologically related to that found both in yeast mitochondria and P. denitrificans. This oxidase appears to be structurally similar to other bacterial aa3-type oxidases, though does not function as a proton pump. The success of the purification of the oxidase critically depends on the purity of the cytoplasmic membrane preparation.",
author = "Angelo Azzi and Gennis, {Robert B}",
year = "1986",
month = "1",
day = "1",
doi = "10.1016/S0076-6879(86)26015-2",
language = "English (US)",
volume = "126",
pages = "138--145",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "C",

}

TY - JOUR

T1 - [13] Purification of the aa3-Type Cytochrome-c Oxidase from Rhodopseudomonas Sphaeroides

AU - Azzi, Angelo

AU - Gennis, Robert B

PY - 1986/1/1

Y1 - 1986/1/1

N2 - This chapter describes the purification of the aa3-type cytochrome-c oxidase from Rhodopseudomonas sphaeroides. The chapter outlines the procedures for the preparation of the bacterial membranes and purification of the oxidase. R. sphaeroides is a nonsulfur bacterium that, when grown anaerobically in the light, develops a photosynthetic apparatus. In the dark and in the presence of oxygen, the photosynthetic apparatus is suppressed and the bacterium utilizes a respiratory chain. The cytochrome-c oxidase from R. sphaeroides can easily and quickly be prepared in small amounts. The enzyme is immunologically related to that found both in yeast mitochondria and P. denitrificans. This oxidase appears to be structurally similar to other bacterial aa3-type oxidases, though does not function as a proton pump. The success of the purification of the oxidase critically depends on the purity of the cytoplasmic membrane preparation.

AB - This chapter describes the purification of the aa3-type cytochrome-c oxidase from Rhodopseudomonas sphaeroides. The chapter outlines the procedures for the preparation of the bacterial membranes and purification of the oxidase. R. sphaeroides is a nonsulfur bacterium that, when grown anaerobically in the light, develops a photosynthetic apparatus. In the dark and in the presence of oxygen, the photosynthetic apparatus is suppressed and the bacterium utilizes a respiratory chain. The cytochrome-c oxidase from R. sphaeroides can easily and quickly be prepared in small amounts. The enzyme is immunologically related to that found both in yeast mitochondria and P. denitrificans. This oxidase appears to be structurally similar to other bacterial aa3-type oxidases, though does not function as a proton pump. The success of the purification of the oxidase critically depends on the purity of the cytoplasmic membrane preparation.

UR - http://www.scopus.com/inward/record.url?scp=0022822131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022822131&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(86)26015-2

DO - 10.1016/S0076-6879(86)26015-2

M3 - Article

C2 - 2856120

AN - SCOPUS:0022822131

VL - 126

SP - 138

EP - 145

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

IS - C

ER -