1-methylthio-d-xylulose 5-phosphate methylsulfurylase: A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum

Benjamin P. Warlick, Bradley S. Evans, Tobias J. Erb, Udipi A. Ramagopal, Jaya Sriram, Heidi J Imker, J. Michael Sauder, Jeffrey B. Bonanno, Stephen K. Burley, F. Robert Tabita, Steven C. Almo, Jonathan V Sweedler, John Alan Gerlt

Research output: Contribution to journalArticle

Abstract

Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.

Original languageEnglish (US)
Pages (from-to)8324-8326
Number of pages3
JournalBiochemistry
Volume51
Issue number42
DOIs
StatePublished - Oct 23 2012

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Rhodospirillum rubrum
Salvaging
S-Adenosylmethionine
Biosynthesis
Terpenes
Polyamines
Adenine
Methionine
xylulose-5-phosphate
Catalytic Domain

ASJC Scopus subject areas

  • Biochemistry

Cite this

1-methylthio-d-xylulose 5-phosphate methylsulfurylase : A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum. / Warlick, Benjamin P.; Evans, Bradley S.; Erb, Tobias J.; Ramagopal, Udipi A.; Sriram, Jaya; Imker, Heidi J; Sauder, J. Michael; Bonanno, Jeffrey B.; Burley, Stephen K.; Tabita, F. Robert; Almo, Steven C.; Sweedler, Jonathan V; Gerlt, John Alan.

In: Biochemistry, Vol. 51, No. 42, 23.10.2012, p. 8324-8326.

Research output: Contribution to journalArticle

Warlick, BP, Evans, BS, Erb, TJ, Ramagopal, UA, Sriram, J, Imker, HJ, Sauder, JM, Bonanno, JB, Burley, SK, Tabita, FR, Almo, SC, Sweedler, JV & Gerlt, JA 2012, '1-methylthio-d-xylulose 5-phosphate methylsulfurylase: A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum', Biochemistry, vol. 51, no. 42, pp. 8324-8326. https://doi.org/10.1021/bi301215g
Warlick, Benjamin P. ; Evans, Bradley S. ; Erb, Tobias J. ; Ramagopal, Udipi A. ; Sriram, Jaya ; Imker, Heidi J ; Sauder, J. Michael ; Bonanno, Jeffrey B. ; Burley, Stephen K. ; Tabita, F. Robert ; Almo, Steven C. ; Sweedler, Jonathan V ; Gerlt, John Alan. / 1-methylthio-d-xylulose 5-phosphate methylsulfurylase : A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum. In: Biochemistry. 2012 ; Vol. 51, No. 42. pp. 8324-8326.
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AU - Gerlt, John Alan

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