1-methylthio-d-xylulose 5-phosphate methylsulfurylase: A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum

Benjamin P. Warlick, Bradley S. Evans, Tobias J. Erb, Udipi A. Ramagopal, Jaya Sriram, Heidi J. Imker, J. Michael Sauder, Jeffrey B. Bonanno, Stephen K. Burley, F. Robert Tabita, Steven C. Almo, Jonathan S. Sweedler, John A. Gerlt

Abstract

Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.

Original languageEnglish (US)
Pages (from-to)8324-8326
Number of pages3
JournalBiochemistry
Volume51
Issue number42
DOIs
StatePublished - Oct 23 2012

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Warlick, B. P., Evans, B. S., Erb, T. J., Ramagopal, U. A., Sriram, J., Imker, H. J., Sauder, J. M., Bonanno, J. B., Burley, S. K., Tabita, F. R., Almo, S. C., Sweedler, J. S., & Gerlt, J. A. (2012). 1-methylthio-d-xylulose 5-phosphate methylsulfurylase: A novel route to 1-deoxy-d-xylulose 5-phosphate in Rhodospirillum rubrum. Biochemistry, 51(42), 8324-8326. https://doi.org/10.1021/bi301215g